Revealing bacterial cell biology using cryo-electron tomography

A protease-mediated switch regulates the growth of magnetosome organelles in Magnetospirillum magneticum

(2022) Juan Wan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Multi-particle cryo-EM refinement with M visualizes ribosome-antibiotic complex at 3.5 Å in cells

(2021) Dimitry Tegunov et al.   NATURE METHODS

FtsZ treadmilling is essential for Z-ring condensation and septal constriction initiation in Bacillus subtilis cell division

(2021) Kevin D. Whitley et al.   Nature Communications

Studying bacterial chemosensory array with CryoEM

(2021) Zhuan Qin et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Architecture of cell–cell junctions in situ reveals a mechanism for bacterial biofilm inhibition

(2021) Charlotte E. Melia et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Asymmetric localization of the cell division machinery during Bacillus subtilis sporulation

(2021) Kanika Khanna et al.   eLife

In situ imaging of bacterial outer membrane projections and associated protein complexes using electron cryo-tomography

(2021) Mohammed Kaplan et al.   eLife

Automated cryo-lamella preparation for high-throughput in-situ structural biology

(2020) Genevieve Buckley et al.   JOURNAL OF STRUCTURAL BIOLOGY

Complete structure of the chemosensory array core signalling unit in an E. coli minicell strain

(2020) Alister Burt et al.   Nature Communications

Preparing samples from whole cells using focused-ion-beam milling for cryo-electron tomography

(2020) Felix R. Wagner et al.   Nature Protocols

Fully automated, sequential focused ion beam milling for cryo-electron tomography

(2020) Tobias Zachs et al.   eLife

Charting the native architecture of Chlamydomonas thylakoid membranes with single-molecule precision

(2020) Wojciech Wietrzynski et al.   eLife

Evolutionary Remodelling of the Cell Envelope in Bacteria of the Planctomycetes Phylum

(2020) Mayank Mahajan et al.   Genome Biology and Evolution

The versatile Pseudomonas aeruginosa biofilm matrix protein CdrA promotes aggregation through different extracellular EPS interactions

(2020) Courtney Reichhardt et al.   JOURNAL OF BACTERIOLOGY

Formation and function of bacterial organelles

(2020) Chris Greening et al.   NATURE REVIEWS MICROBIOLOGY

Cryogenic single-molecule fluorescence annotations for electron tomography reveal in situ organization of key proteins in Caulobacter

(2020) Peter D. Dahlberg et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

In-cell architecture of an actively transcribing-translating expressome

(2020) Francis J. O’Reilly et al.   SCIENCE

Cryo-Electron Tomography Reveals the Complex Ultrastructural Organization of Multicellular Filamentous Chloroflexota (Chloroflexi) Bacteria

(2020) Vasil A. Gaisin et al.   Frontiers in Microbiology

Exploring the bacterial nano-universe

(2020) Niklas Söderholm et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Molecular mechanism for rotational switching of the bacterial flagellar motor

(2020) Yunjie Chang et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Structure of the Bacterial Cellulose Ribbon and Its Assembly-Guiding Cytoskeleton by Electron Cryotomography

(2020) William J. Nicolas et al.   JOURNAL OF BACTERIOLOGY

FtsZ dynamics in bacterial division: What, how, and why?

(2020) Jordan M. Barrows et al.   CURRENT OPINION IN CELL BIOLOGY

Molecular Logic of Prokaryotic Surface Layer Structures

(2020) Tanmay A.M. Bharat et al.   TRENDS IN MICROBIOLOGY

In situ and high‐resolution cryo‐EM structure of a bacterial type VI secretion system membrane complex

(2019) Chiara Rapisarda et al.   EMBO JOURNAL

Viral Capsid Trafficking along Treadmilling Tubulin Filaments in Bacteria

(2019) Vorrapon Chaikeeratisak et al.   CELL

Structure and Function of a Bacterial Gap Junction Analog

(2019) Gregor L. Weiss et al.   CELL

A cryo-FIB lift-out technique enables molecular-resolution cryo-ET within native Caenorhabditis elegans tissue

(2019) Miroslava Schaffer et al.   NATURE METHODS

Bidirectional contraction of a type six secretion system

(2019) Piotr Szwedziak et al.   Nature Communications

In situ structure and assembly of the multidrug efflux pump AcrAB-TolC

(2019) Xiaodong Shi et al.   Nature Communications

Structural insights into flagellar stator–rotor interactions

(2019) Yunjie Chang et al.   eLife

PIE-scope, integrated cryo-correlative light and FIB/SEM microscopy

(2019) Sergey Gorelick et al.   eLife

The molecular architecture of engulfment during Bacillus subtilis sporulation

(2019) Kanika Khanna et al.   eLife

Diversity of Bacterial Chemosensory Arrays

(2019) Wen Yang et al.   TRENDS IN MICROBIOLOGY

Toward high-resolution in situ structural biology with cryo-electron tomography and subtomogram averaging

(2019) Florian KM Schur   CURRENT OPINION IN STRUCTURAL BIOLOGY

A bacteriophage nucleus-like compartment shields DNA from CRISPR nucleases

(2019) Senén D. Mendoza et al.   NATURE

In situ Microfluidic Cryofixation for Cryo Focused Ion Beam Milling and Cryo Electron Tomography

(2019) Marie Fuest et al.   Scientific Reports

Locating macromolecules and determining structures inside bacterial cells using electron cryotomography

(2018) Charlotte E. Melia et al.   BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS

Imaging bacteria inside their host by cryo-focused ion beam milling and electron cryotomography

(2018) João M Medeiros et al.   CURRENT OPINION IN MICROBIOLOGY

Connectivity of Centermost Chromatophores in Rhodobacter sphaeroides Bacteria

(2018) Jade M. Noble et al.   MOLECULAR MICROBIOLOGY

Prokaryotic cytoskeletons: protein filaments organizing small cells

(2018) James Wagstaff et al.   NATURE REVIEWS MICROBIOLOGY

CryoEM of bacterial secretion systems

(2018) Romain Kooger et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

Visualizing Individual RuBisCO and Its Assembly into Carboxysomes in Marine Cyanobacteria by Cryo-Electron Tomography

(2018) Wei Dai et al.   JOURNAL OF MOLECULAR BIOLOGY

Visualization of the type III secretion mediated Salmonella–host cell interface using cryo-electron tomography

(2018) Donghyun Park et al.   eLife

Ultrastructural analysis of bacteriophage Φ29 during infection of Bacillus subtilis

(2017) Madeline M. Farley et al.   JOURNAL OF STRUCTURAL BIOLOGY

Assembly of a nucleus-like structure during viral replication in bacteria

(2017) Vorrapon Chaikeeratisak et al.   SCIENCE

Quantitative Analysis of Filament Branch Orientation in Listeria Actin Comet Tails

(2016) Marion Jasnin et al.   BIOPHYSICAL JOURNAL

Biofilms: an emergent form of bacterial life

(2016) Hans-Curt Flemming et al.   NATURE REVIEWS MICROBIOLOGY

A new view into prokaryotic cell biology from electron cryotomography

(2016) Catherine M. Oikonomou et al.   NATURE REVIEWS MICROBIOLOGY

Architecture of the type IVa pilus machine

(2016) Yi-Wei Chang et al.   SCIENCE

Bacterial cellulose biosynthesis: diversity of operons, subunits, products, and functions

(2015) Ute Römling et al.   TRENDS IN MICROBIOLOGY

Structure of a type IV pilus machinery in the open and closed state

(2015) Vicki AM Gold et al.   eLife

Fluorescence Imaging for Bacterial Cell Biology: From Localization to Dynamics, From Ensembles to Single Molecules

(2014) Zhizhong Yao et al.   Annual Review of Microbiology

Visualization of channels connecting cells in filamentous nitrogen-fixing cyanobacteria

(2014) Amin Omairi-Nasser et al.   FASEB JOURNAL

Bacterial solutions to multicellularity: a tale of biofilms, filaments and fruiting bodies

(2014) Dennis Claessen et al.   NATURE REVIEWS MICROBIOLOGY

High throughput 3D super-resolution microscopy reveals Caulobacter crescentus in vivo Z-ring organization

(2014) S. J. Holden et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Controlled Bacterial Lysis for Electron Tomography of Native Cell Membranes

(2014) Xiaofeng Fu et al.   STRUCTURE

Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell division

(2014) Piotr Szwedziak et al.   eLife

A Phage Tubulin Assembles Dynamic Filaments by an Atypical Mechanism to Center Viral DNA within the Host Cell

(2012) James A. Kraemer et al.   CELL

Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins

(2012) A. Briegel et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A Ferritin-Based Label for Cellular Electron Cryotomography

(2011) Qing Wang et al.   STRUCTURE

CaulobacterPopZ forms a polar subdomain dictating sequential changes in pole composition and function

(2010) Grant R. Bowman et al.   MOLECULAR MICROBIOLOGY

Direct Visualization of the Outer Membrane of Mycobacteria and Corynebacteria in Their Native State

(2008) B. Zuber et al.   JOURNAL OF BACTERIOLOGY