Article
Physiology
Olivia Spitz, Isabelle N. Erenburg, Kerstin Kanonenberg, Sandra Peherstorfer, Michael H. H. Lenders, Jens Reiners, Miao Ma, Ben F. Luisi, Sander H. J. Smits, Lutz Schmitt
Summary: The toxin hemolysin A is secreted by a dedicated secretion machinery in uropathogenic E. coli strains. This machinery, consisting of TolC, HlyD and HlyB proteins, belongs to the Type I secretion system family. Researchers proposed an amphipathic helix in the C-terminal region of the toxin that plays a crucial role in the early steps of secretion.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Julie Ming Liang, Lisa Ann Burdette, Han Teng Wong, Danielle Tullman-Ercek
Summary: Protein production using bacteria is costly and inefficient. Researchers have engineered a bacterial secretion system to improve the production process by directly secreting soluble proteins into the culture media. They explored different activation methods and created strains capable of efficiently producing heterologous proteins. They also discovered a genetic relationship between T3SS regulators, hilE and hilD, further enhancing the industrial competitiveness of protein production strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Applied
Evgeny V. Pospelov, Alexander V. Zhirov, Baglan Kamidolla, Alexey Yu. Sukhorukov
Summary: A protocol was developed for the synthesis of 2,3-dihydrofurans by reductive denitrogenation/ring contraction of cyclic nitronates. Cheap Raney nickel was used as both catalyst and stoichiometric agent, eliminating the need for high-pressure equipment. The products were obtained in high purity and often didn't require column chromatography. Studies on substrate scope, functional group tolerance, and mechanism were conducted. This approach provides a straightforward two-step access to densely substituted 2,3-dihydrofurans from nitrostyrenes and alkenes.
ADVANCED SYNTHESIS & CATALYSIS
(2023)
Review
Microbiology
Anna Shaliutina-Loginova, Olivera Francetic, Pavel Dolezal
Summary: Bacteria have evolved sophisticated protein secretion nanomachines over billions of years to deliver toxins, enzymes, and proteins. The type II secretion system (T2SS) is used by Gram-negative bacteria to export folded proteins across the outer membrane. Recent findings have shown that components of the T2SS can be found in the mitochondria of certain eukaryotic lineages, suggesting the presence of a mitochondrial T2SS-derived system (miT2SS). This review discusses recent advances in the field and raises questions about the function and evolution of miT2SSs.
Article
Microbiology
Steven J. Hersch, Linh Lam, Tao G. Dong
Summary: Genetic editing has been revolutionized by delivering Cre recombinase as a protein through engineered bacterial type six secretion system (T6SS). The versatile T6SS system can also deliver other exogenous effectors, like TseC, enhancing its efficacy against specific pathogens. This engineered system shows promise in microbiome manipulation and as a next-generation antimicrobial tool.
Article
Biotechnology & Applied Microbiology
Zohreh N. Pourhassan, Haiyang Cui, Neele Muckhoff, Mehdi D. Davari, Sander H. J. Smits, Ulrich Schwaneberg, Lutz Schmitt
Summary: Protein secretion is advantageous for recombinant protein production. Type 1 secretion systems (T1SS) have a simple architecture and are attractive for biotechnological applications. The hemolysin A type 1 secretion system (HlyA T1SS) from Escherichia coli has been used for secretion of heterologous proteins, but its commercial utility is limited by low secretion titers. To improve this, we engineered the inner membrane complex of the system using KnowVolution strategy, resulting in a novel HlyB variant with improved secretion for two hydrolases.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Patricia Bernal, R. Christopher D. Furniss, Selina Fecht, Rhoda C. Y. Leung, Livia Spiga, Despoina A. Mavridou, Alain Filloux
Summary: The T6SS is a nanomachine primarily involved in interbacterial competition, with TssA being a key component for its assembly. Researchers have found unexpected diversity in TssA proteins, with a short form and a long form existing. The discovery of structural components that interact with short TssA proteins sheds light on the mechanisms for T6SS assembly and functioning.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Microbiology
Kevin Munoz Navarrete, Ladislav Bumba, Tatyana Prudnikova, Ivana Malcova, Tania Romero Allsop, Peter Sebo, Jana Kamanova
Summary: The study reveals that BopN is a gatekeeper of the Bordetella T3SS, responsible for regulating the secretion and translocation of the BteA effector into host cells. It is similar to gatekeepers that control access to the T3SS channel from the bacterial cytoplasm. Deletion of the bopN gene in B. bronchiseptica increased secretion of BteA, but decreased the translocation of BteA into host cell cytoplasm.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Nidhi Singh, Thales Kronenberger, Andrea Eipper, Felix Weichel, Mirita Franz-Wachtel, Boris Macek, Samuel Wagner
Summary: The study found that the salt bridges in the assembly of virulence-associated T3SS are not critical, but facilitate the incorporation of the next subunit. The conserved charged residues are crucial for type III-dependent secretion and play a key role in the function of T3SS.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Stephan Wimmi, Alexander Balinovic, Hannah Jeckel, Lisa Selinger, Dimitrios Lampaki, Emma Eisemann, Ina Meuskens, Dirk Linke, Knut Drescher, Ulrike Endesfelder, Andreas Diepold
Summary: Current research shows that in gastrointestinal pathogens, the external pH plays a crucial role in regulating the assembly of T3SS cytosolic components, preventing T3SS activity in the stomach and facilitating its reactivation in the intestine.
NATURE COMMUNICATIONS
(2021)
Article
Biotechnology & Applied Microbiology
Sun-Young Kim, Jennifer K. K. Parker, Monica Gonzalez-Magaldi, Mady S. S. Telford, Daniel J. J. Leahy, Bryan W. W. Davies
Summary: Small proteins with various functions can be secreted by Gram-negative bacteria through a specific type I secretion system. This study shows that the microcin V type I system can secrete a wide range of natural and synthetic small proteins, regardless of their chemical properties, as long as the protein length is within the system's capacity. These findings have implications for small-protein research and delivery in Gram-negative bacteria.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)
Review
Microbiology
Lisa Bowman, Tracy Palmer
Summary: The T7SS of Staphylococcus aureus plays a crucial role in virulence in disease models and intraspecies competition, with its genes located at the ess locus encoding multiple substrate recognition proteins. T7SS is widely conserved across staphylococci, encoding various toxin and immunity genes, while genomic islands encoding multiple immunity proteins in species lacking T7SS suggest a significant role for the secretion system in bacterial antagonism.
ANNUAL REVIEW OF MICROBIOLOGY, VOL 75, 2021
(2021)
Article
Microbiology
N. Zohreh Pourhassan, Eymen Hachani, Olivia Spitz, Sander H. J. Smits, Lutz Schmitt
Summary: This study investigates the role of the ABC transporter hemolysin B (HlyB) in the HlyA secretion system. The results reveal that the HlyA T1SS is composed of multiple proteins, and the GG repeats and amphipathic helix are crucial for efficient secretion.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Fisheries
Zhen Tao, Haoda Ye, Chaozheng Zhang, Suming Zhou, Kequan Wang, Mingming Zhang, Jianjun Xie, Hetron Mweemba Munang'andu, Cheng Xu, Pengcheng Wang, Xiaojun Yan
Summary: In this study, a novel two-component system PvgAS has been identified in P. plecoglossicida, which regulates the expression of T6SS-1 and plays a crucial role in bacterial pathogenicity. This finding provides important insights into the understanding of the pathogenic mechanisms of P. plecoglossicida and potential therapeutic interventions against this bacterium.
Article
Microbiology
Chih-Feng Wu, Alexandra J. Weisberg, Edward W. Davis, Lin Chou, Surtaz Khan, Erh-Min Lai, Chih-Horng Kuo, Jeff H. Chang
Summary: The T6SS is used by many Gram-negative bacteria for interbacterial competition, with diverse strains of agrobacteria showing different patterns of T6SS expression and enrichment in specific sets of T6SS loci. Diversification is driven by the reshuffling of entire loci and fragments, but constrained by the maintenance of specific gene subtypes.
Article
Biochemistry & Molecular Biology
Patrick J. Lariviere, Piotr Szwedziak, Christopher R. Mahone, Jan Loewe, Erin D. Goley
MOLECULAR MICROBIOLOGY
(2018)
Article
Biology
Saman Hussain, Carl N. Wivagg, Piotr Szwedziak, Felix Wong, Kaitlin Schaefer, Thierry Izore, Lars D. Renner, Matthew J. Holmes, Yingjie Sun, Alexandre W. Bisson-Filho, Suzanne Walker, Ariel Amir, Jan Lowe, Ethan C. Garner
Article
Biochemistry & Molecular Biology
Romain Kooger, Piotr Szwedziak, Desiree Bock, Martin Pilhofer
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Piotr Szwedziak, Qing Wang, Stefan M. V. Freund, Jan Loewe
Article
Biology
Piotr Szwedziak, Qing Wang, Tanmay A. M. Bharat, Matthew Tsim, Jan Loewe
Article
Chemistry, Multidisciplinary
Yao Tian, Ruina Liang, Amit Kumar, Piotr Szwedziak, John H. Viles
Summary: Aβ assemblies have been shown to bind to lipid bilayers, disrupting membrane integrity and potentially leading to Alzheimer's disease. Different forms of Aβ interact with membranes in varying ways, with oligomers and curvilinear protofibrils forming networks on the upper-leaflet of the bilayer, while monomeric and fibrillar Aβ have less impact. Incorporation of Aβ oligomers and protofibrils into lipid bilayers suggests a mechanism for membrane integrity loss.