Article
Multidisciplinary Sciences
Haidai Hu, Philipp F. Popp, Monica Santiveri, Aritz Roa-Eguiara, Yumeng Yan, Freddie J. O. Martin, Zheyi Liu, Navish Wadhwa, Yong Wang, Marc Erhardt, Nicholas M. I. Taylor
Summary: In this study, the structure of Vibrio PomAB was determined by cryo-electron microscopy, revealing sodium binding sites, and a mechanism for ion translocation and selectivity was proposed through functional experiments and molecular dynamics simulations. The involvement of a dynamic helical motif in PomA was suggested to regulate the distance between PomA subunit cytoplasmic domains, stator unit activation, and torque transmission. Overall, this study provides mechanistic insights into ion selectivity and rotor incorporation of the stator unit of the bacterial flagellum.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Pratibha Kumari, Dhiman Ghosh, Agathe Vanas, Yanick Fleischmann, Thomas Wiegand, Gunnar Jeschke, Roland Riek, Cedric Eichmann
Summary: This study investigated the interaction between monomeric alpha-Syn and its fibrillar form using NMR and electron paramagnetic resonance spectroscopy, revealing that intermolecular interactions reduce intramolecular contacts in monomeric alpha-Syn, leading to further unfolding of its intrinsically disordered states and critically contributing to the aggregation kinetics of alpha-Syn during secondary nucleation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Engineering, Aerospace
Yun Zheng, Qingzhe Gao, Hui Yang
Summary: This study investigates the forced response of a compressor blade induced by upstream and downstream stator disturbances as well as rotor-stator interactions using decoupled and coupled configurations. The results show that the dominant excitation for the R1 response induced by upstream disturbances is the wake from the inlet guide vane (IGV), while the cut-on S1-R1 interaction mode is dominant for the response induced by downstream disturbances. The use of the decoupled method without considering the influence of downstream disturbances leads to inaccurate predictions. The formation process of rotor-stator interactions determines whether the decoupled or coupled method should be used, highlighting the importance of including downstream stator vanes.
Article
Chemistry, Medicinal
Daniela Trisciuzzi, Lydia Siragusa, Massimo Baroni, Ida Autiero, Orazio Nicolotti, Gabriele Cruciani
Summary: Peptide-protein interactions play a key role in the onset of diseases. However, the molecular details behind these interactions are still challenging to understand. In this study, a comprehensive in silico study was conducted to investigate the morphology and energetics of peptide binding sites from both the peptide and protein perspectives. The findings reveal physicochemical and structural correlations in peptide-protein interactions.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2022)
Review
Biochemistry & Molecular Biology
Hong-Yan Lin, Jin Dong, Jiangqing Dong, Wen-Chao Yang, Guang-Fu Yang
Summary: 4-Hydroxyphenylpyruvate dioxygenase (HPPD) is a crucial enzyme in tyrosine metabolism and a potential target for herbicide and drug discovery. This study summarizes the structures of HPPD complexed with various molecules and analyzes the inhibitory mechanisms of different inhibitors. By identifying key structural factors, the study provides insights into the design of new inhibitors with enhanced potency and selectivity.
TRENDS IN BIOCHEMICAL SCIENCES
(2023)
Article
Biology
Amandeep S. Arora, Hsiang-Ling Huang, Ramanpreet Singh, Yoshie Narui, Andrejus Suchenko, Tomoyuki Hatano, Sarah M. Heissler, Mohan K. Balasubramanian, Krishna Chinthalapudi, Pekka Lappalainen
Summary: The structures of actin isoforms have been determined at high resolution, revealing their specific conformations and isoform-specific interfaces. These findings provide important insights into the function of actin isoforms and contribute to our overall understanding of cytoskeletal physiology.
Article
Biochemistry & Molecular Biology
Weiyan Sun, Fuchen Luan, Jiajia Wang, Lin Ma, Xiuxiu Li, Gongxian Yang, Chenyang Hao, Xiaochun Qin, Shishang Dong
Summary: The interactions between the VP30 C-terminal domain (CTD) of Lloviu virus (LLOV) and its nucleoprotein (NP) fragment were confirmed, and the crystal structure of the chimeric dimeric LLOV NP-VP30 CTD was determined. This provides a preliminary model for investigating interactions between LLOV VP30 and NP and suggests a potential target for anti-filovirus drug development.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Shivam Tiwari, Vasista Adupa, Dhanesh Sing Das, K. Anki Reddy, Tadikonda Venkata Bharat
Summary: This study provides atomistic insights into the interaction between the spike protein (S) of SARS-CoV-2 and a Montmorillonite (MMT) nano clay surface. The findings highlight the role of electrostatic and hydrophobic interactions in governing this interaction. The study also identifies specific residues and glycans involved in cell entry and immune evasion, which could have implications for future therapeutic techniques.
Article
Multidisciplinary Sciences
Ruben Perez-Carrasco, Maria-Jose Franco-Onate, Jean-Charles Walter, Jerome Dorignac, Fred Geniet, John Palmeri, Andrea Parmeggiani, Nils-Ole Walliser, Ashley L. Nord
Summary: This article focuses on the dynamics of bacterial flagellar motors, revealing a stoichiometry-dependent asymmetry in stator remodeling kinetics and proposing a two-state catch-bond mechanism to explain the observed asymmetry.
Article
Multidisciplinary Sciences
Wentong Jiang, Sanduo Zheng
Summary: This study reports the cryoelectron microscopy structures of the GALR1-G(o) and GALR2-G(q) complexes bound to the endogenous ligand galanin or spexin, revealing the structural determinants of GALRs and highlighting the potential role of zinc ion as a modulator of GALR1.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Review
Immunology
Sreekanth Rajan, Ho Sup Yoon
Summary: Malaria, a prevalent infectious disease, poses a serious challenge due to drug-resistant strains. Adopting a chemo-genomics approach can uncover novel targets and mechanisms of action for better drug targeting. Immunosuppressive drugs have been found to inhibit the growth of the malarial parasite, with peptidyl prolyl cis/trans isomerases (PPIases) as the specific targets. This review summarizes the structural insights on Plasmodium PPIases, their inhibitor complexes, and perspectives on drug discovery.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Hiroyuki Terashima, Seiji Kojima, Michio Hommaa
Summary: The study directly demonstrates the physical interaction between specific residues in FliG and PomA/MotA using site-directed photo-cross-linking and disulfide cross-linking methods. The results provide insight into how stator units assemble around the rotor and generate torque in the bacterial flagellar motor.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jana Omar, Efrat Rosenbaum, Adi Efergan, Bayan Abu Sneineh, Adva Yeheskel, Yuto Maruta, Mitsunori Fukuda, Ronit Sagi-Eisenberg
Summary: This study reveals that Rab12 negatively regulates MC exocytosis by interacting with RILP family effectors. By investigating the interactions between Rab12 and RILP, RILP-L1, and RILP-L2, the researchers proposed a model for the Rab12-RILP complex, providing insights into the regulation of MC secretory granule transport and offering potential tools for understanding Rab12 functions.
SCIENTIFIC REPORTS
(2021)
Review
Biochemistry & Molecular Biology
A. Manuel Liaci, Friedrich Forster
Summary: Cleavable and non-cleavable signal sequences target a quarter of the human proteome to the ER. Interaction between these signal sequences and ER targeting machinery determines protein location and translocation fidelity, as discussed in recent insights from structural biology.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Thermodynamics
Gustavo J. R. Otero, Stephan H. H. J. Smit, Rene Pecnik
Summary: Organic Rankine cycle (ORC) systems have low thermal performance due to the low temperature of heat sources and the inefficiency of the expanders used. Designing turboexpanders is challenging because of the highly supersonic and unsteady flow field.
Article
Microbiology
James P. Phelan, Aurelie Kern, Meghan E. Ramsey, Maureen E. Lundt, Bijaya Sharma, Tao Lin, Lihui Gao, Steven J. Norris, Jenny A. Hyde, Jon T. Skare, Linden T. Hu
Editorial Material
Microbiology
Steven J. Norris
JOURNAL OF BACTERIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Hui Xu, Jun He, Jun Liu, Md A. Motaleb
MOLECULAR MICROBIOLOGY
(2020)
Article
Microbiology
Diane G. Edmondson, Gary P. Wormser, Steven J. Norris
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2020)
Article
Microbiology
Beth Hahn, Phillip Anderson, Zouyan Lu, Rebecca Danner, Zhipeng Zhou, Noorie Hyun, Lihui Gao, Tao Lin, Steven J. Norris, Jenifer Coburn
Article
Biochemistry & Molecular Biology
Yunjie Chang, Kai Zhang, Brittany L. Carroll, Xiaowei Zhao, Nyles W. Charon, Steven J. Norris, Md A. Motaleb, Chunhao Li, Jun Liu
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Microbiology
Yan Zhang, Tong Chen, Sajith Raghunandanan, Xuwu Xiang, Jing Yang, Qiang Liu, Diane G. Edmondson, Steven J. Norris, X. Frank Yang, Yongliang Lou
Article
Microbiology
Diane G. Edmondson, Bridget D. DeLay, Lindsay E. Kowis, Steven J. Norris
Summary: The bacterium that causes syphilis, Treponema pallidum subsp. pallidum, has been successfully cultured in vitro for over 3 years using a system of coculture with Sf1Ep rabbit epithelial cells in TpCM-2 medium and a low-oxygen environment. Specific requirements for sustained growth and multiplication, such as the presence of Sf1Ep cells and certain basal media, have been identified.
Article
Biochemistry & Molecular Biology
Nadja Leimer, Xiaoqian Wu, Yu Imai, Madeleine Morrissette, Norman Pitt, Quentin Favre-Godal, Akira Iinishi, Samta Jain, Mariaelena Caboni, Inga V. Leus, Vincent Bonifay, Samantha Niles, Rachel Bargabos, Meghan Ghiglieri, Rachel Corsetti, Megan Krumpoch, Gabriel Fox, Sangkeun Son, Dorota Klepacki, Yury S. Polikanov, Cecily A. Freliech, Julie E. McCarthy, Diane G. Edmondson, Steven J. Norris, Anthony D'Onofrio, Linden T. Hu, Helen I. Zgurskaya, Kim Lewis
Summary: The research found that Hygromycin A can selectively eliminate B. burgdorferi, curing infections in mice, and causing less disruption to the host microbiome.
Article
Microbiology
Bridget D. De Lay, Todd A. Cameron, Nicholas R. De Lay, Steven J. Norris, Diane G. Edmondson
Summary: Comparing the RNA transcript profiles of Treponema pallidum in in vitro culture and rabbit infection, it was found that overall gene expression patterns were similar, but there were significant differences in the expression levels of some genes between these two environments.
Article
Microbiology
Hui Xu, Bo Hu, David A. Flesher, Jun Liu, Md A. Motaleb
Summary: This study investigates the assembly process of bacterial flagellar components and the functions of related proteins in various bacteria, highlighting the crucial role of BB0259 in spirochetes and the essentiality of residue E580 for its PG-hydrolyzing activity.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Shuaiqi Guo, Hui Xu, Yunjie Chang, Md A. Motaleb, Jun Liu
Summary: This study reveals the interaction between the stator complex and the FliL ring in bacterial flagellar motors. The research shows that upon recruitment of stator units, FliL forms a full ring and stabilizes the conformation of the stator complex through its interaction with MotB, leading to increased torque generation. Additionally, the study provides evidence that FliL can regulate the assembly of stator complexes, thereby influencing stator and motor function.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Microbiology
Irene N. Kasumba, Kit Tilly, Tao Lin, Steven J. Norris, Patricia A. Rosa
Summary: The main finding of this study challenges the assumption that a unique gene present in the Lyme disease spirochete, Borrelia burgdorferi, plays a critical role in the murine host or tick vector. The study suggests that the current laboratory model of the infectious cycle of the Lyme disease spirochete is inadequate. The study also highlights the importance of complementation in genetic studies of Borrelia burgdorferi to accurately interpret mutant phenotypes.
MICROBIOLOGY SPECTRUM
(2023)