Insect Cells for High-Yield Production of SARS-CoV-2 Spike Protein: Building a Virosome-Based COVID-19 Vaccine Candidate

A SARS-CoV-2 Wuhan spike virosome vaccine induces superior neutralization breadth compared to one using the Beta spike

(2022) Yme U. van der Velden et al.   Scientific Reports

Production of high‐quality SARS‐CoV‐2 antigens: impact of bioprocess and storage on glycosylation, biophysical attributes, and ELISA serologic tests performance

(2021) Rute Castro et al.   BIOTECHNOLOGY AND BIOENGINEERING

Virus vaccines: proteins prefer prolines

(2021) Rogier W. Sanders et al.   Cell Host & Microbe

Covid-19: Novavax vaccine efficacy is 86% against UK variant and 60% against South African variant

(2021) Elisabeth Mahase   BMJ-British Medical Journal

Glycosylation is a key in SARS-CoV-2 infection

(2021) Celso A. Reis et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Two-Component Nanoparticle Vaccine Displaying Glycosylated Spike S1 Domain Induces Neutralizing Antibody Response against SARS-CoV-2 Variants

(2021) Linda van Oosten et al.   mBio

A Novel Coronavirus from Patients with Pneumonia in China, 2019

(2020) Na Zhu et al.   NEW ENGLAND JOURNAL OF MEDICINE

Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation

(2020) Daniel Wrapp et al.   SCIENCE

Functional assessment of cell entry and receptor usage for SARS-CoV-2 and other lineage B betacoronaviruses

(2020) Michael Letko et al.   Nature Microbiology

Vaccination with virosomally formulated recombinant CyRPA elicits protective antibodies against Plasmodium falciparum parasites in preclinical in vitro and in vivo models

(2020) Marco Tamborrini et al.   npj Vaccines

A serological assay to detect SARS-CoV-2 seroconversion in humans

(2020) Fatima Amanat et al.   NATURE MEDICINE

Site-specific glycan analysis of the SARS-CoV-2 spike

(2020) Yasunori Watanabe et al.   SCIENCE

A highly conserved cryptic epitope in the receptor binding domains of SARS-CoV-2 and SARS-CoV

(2020) Meng Yuan et al.   SCIENCE

New GMP manufacturing processes to obtain thermostable HIV-1 gp41 virosomes under solid forms for various mucosal vaccination routes

(2020) Mario Amacker et al.   npj Vaccines

A thermostable, closed SARS-CoV-2 spike protein trimer

(2020) Xiaoli Xiong et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Potent neutralizing antibodies from COVID-19 patients define multiple targets of vulnerability

(2020) Philip J. M. Brouwer et al.   SCIENCE

Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays

(2020) Dominic Esposito et al.   PROTEIN EXPRESSION AND PURIFICATION

Structural analysis of full-length SARS-CoV-2 spike protein from an advanced vaccine candidate

(2020) Sandhya Bangaru et al.   SCIENCE

Improving Influenza HA-Vlps Production in Insect High Five Cells via Adaptive Laboratory Evolution

(2020) Ricardo Correia et al.   Vaccines

Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells

(2020) Matthew Stuible et al.   JOURNAL OF BIOTECHNOLOGY

COVID-19: Coronavirus Vaccine Development Updates

(2020) Jing Zhao et al.   Frontiers in Immunology

Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis

(2020) Natalia G. Herrera et al.   ACS Omega

Enabling PAT in insect cell bioprocesses: In situ monitoring of recombinant adeno‐associated virus production by fluorescence spectroscopy

(2019) Daniel A. M. Pais et al.   BIOTECHNOLOGY AND BIOENGINEERING

Immunogenicity assessment of biotherapeutic products: An overview of assays and their utility

(2015) Meenu Wadhwa et al.   BIOLOGICALS

Formulation screening by differential scanning fluorimetry: how often does it work?

(2015) Marko Ristic et al.   Acta Crystallographica Section F-Structural Biology Communications

Thirty years of baculovirus-insect cell protein expression: from dark horse to mainstream technology

(2014) M. M. van Oers et al.   JOURNAL OF GENERAL VIROLOGY

Technology transfer and scale-up of the Flublok® recombinant hemagglutinin (HA) influenza vaccine manufacturing process

(2014) Barry Buckland et al.   VACCINE

Influenza virosomes as vaccine adjuvant and carrier system

(2013) Christian Moser et al.   Expert Review of Vaccines

Effects of sugars on the thermal stability of a protein

(2013) Hiraku Oshima et al.   JOURNAL OF CHEMICAL PHYSICS

Strategies for the Assessment of Protein Aggregates in Pharmaceutical Biotech Product Development

(2010) John den Engelsman et al.   PHARMACEUTICAL RESEARCH

Mechanisms of Protein Stabilization and Prevention of Protein Aggregation by Glycerol

(2009) Vincent Vagenende et al.   BIOCHEMISTRY

Error assessment in recombinant baculovirus titration: Evaluation of different methods

(2009) António Roldão et al.   JOURNAL OF VIROLOGICAL METHODS

Eleven years of Inflexal® V—a virosomal adjuvanted influenza vaccine

(2009) Christian Herzog et al.   VACCINE

Epaxal®: a virosomal vaccine to prevent hepatitis A infection

(2008) Patrick A Bovier   Expert Review of Vaccines