Rapid, high-yield production of full-length SARS-CoV-2 spike ectodomain by transient gene expression in CHO cells

Cryo-EM structure of the 2019-nCoV spike in the prefusion conformation

(2020) Daniel Wrapp et al.   SCIENCE

Site-specific glycan analysis of the SARS-CoV-2 spike

(2020) Yasunori Watanabe et al.   SCIENCE

A thermostable, closed SARS-CoV-2 spike protein trimer

(2020) Xiaoli Xiong et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Optimizing high-yield production of SARS-CoV-2 soluble spike trimers for serology assays

(2020) Dominic Esposito et al.   PROTEIN EXPRESSION AND PURIFICATION

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

(2020) Alexandra C. Walls et al.   CELL

Effect of Fc Fusion on Folding and Immunogenicity of Middle East Respiratory Syndrome Coronavirus Spike Protein

(2019) Jungmin Chun et al.   JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY

Structural basis for human coronavirus attachment to sialic acid receptors

(2019) M. Alejandra Tortorici et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Optimization of a high-cell-density polyethylenimine transfection method for rapid protein production in CHO-EBNA1 cells

(2018) Matthew Stuible et al.   JOURNAL OF BIOTECHNOLOGY

Beyond preclinical research: production of CHO-derived biotherapeutics for toxicology and early-phase trials by transient gene expression or stable pools

(2018) Matthew Stuible et al.   Current Opinion in Chemical Engineering

Rapid protein production from stable CHO cell pools using plasmid vector and the cumate gene-switch

(2017) Adeline Poulain et al.   JOURNAL OF BIOTECHNOLOGY

Cryo-electron microscopy structures of the SARS-CoV spike glycoprotein reveal a prerequisite conformational state for receptor binding

(2016) Miao Gui et al.   CELL RESEARCH

Human Coronavirus HKU1 Spike Protein UsesO-Acetylated Sialic Acid as an Attachment Receptor Determinant and Employs Hemagglutinin-Esterase Protein as a Receptor-Destroying Enzyme

(2015) Xingchuan Huang et al.   JOURNAL OF VIROLOGY

Purified coronavirus spike protein nanoparticles induce coronavirus neutralizing antibodies in mice

(2014) Christopher M. Coleman et al.   VACCINE

Immunogenicity and Protection Efficacy of Monomeric and Trimeric Recombinant SARS Coronavirus Spike Protein Subunit Vaccine Candidates

(2013) Jie Li et al.   VIRAL IMMUNOLOGY

A 219-mer CHO-Expressing Receptor-Binding Domain of SARS-CoV S Protein Induces Potent Immune Responses and Protective Immunity

(2010) Lanying Du et al.   VIRAL IMMUNOLOGY