Sequential development of several RT‐qPCR tests using LNA nucleotides and dual probe technology to differentiate SARS‐CoV‐2 from influenza A and B

Two-step strategy for the identification of SARS-CoV-2 variant of concern 202012/01 and other variants with spike deletion H69–V70, France, August to December 2020

(2021) Antonin Bal et al.   Eurosurveillance

Genetic Variants of SARS-CoV-2—What Do They Mean?

(2021) Adam S. Lauring et al.   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

One Year of SARS-CoV-2: How Much Has the Virus Changed?

(2021) Santiago Vilar et al.   Biology-Basel

Self-collected Saline Gargle Samples as an Alternative to Healthcare Worker Collected Nasopharyngeal Swabs for COVID-19 Diagnosis in Outpatients

(2021) David M. Goldfarb et al.   JOURNAL OF CLINICAL MICROBIOLOGY

RT-qPCR Diagnostics: The “Drosten” SARS-CoV-2 Assay Paradigm

(2021) Stephen Bustin et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

A SARS-CoV-2 mutant from B.1.258 lineage with ∆H69/∆V70 deletion in the Spike protein circulating in Central Europe in the fall 2020

(2021) Broňa Brejová et al.   VIRUS GENES

Surveillance of SARS-CoV-2 lineage B.1.1.7 in Slovakia using a novel, multiplexed RT-qPCR assay

(2021) Kristína Boršová et al.   Scientific Reports

Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR

(2020) Victor M Corman et al.   Eurosurveillance

Limits of Detection of 6 Approved RT–PCR Kits for the Novel SARS-Coronavirus-2 (SARS-CoV-2)

(2020) Xueliang Wang et al.   CLINICAL CHEMISTRY

Authors’ response: SARS-CoV-2 detection by real-time RT-PCR

(2020) Victor M Corman et al.   Eurosurveillance

Letter to the editor: SARS-CoV-2 detection by real-time RT-PCR

(2020) Trestan Pillonel et al.   Eurosurveillance

Rates of Co-infection Between SARS-CoV-2 and Other Respiratory Pathogens

(2020) David Kim et al.   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

Comparative Performance of SARS-CoV-2 Detection Assays Using Seven Different Primer-Probe Sets and One Assay Kit

(2020) Arun K. Nalla et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Co-infection of SARS-CoV-2 and Influenza virus in Early Stage of the COVID-19 Epidemic in Wuhan, China

(2020) Xin Zheng et al.   JOURNAL OF INFECTION

SARS-CoV-2 and influenza virus co-infection

(2020) Elena Cuadrado-Payán et al.   LANCET

Development of a Laboratory-safe and Low-cost Detection Protocol for SARS-CoV-2 of the Coronavirus Disease 2019 (COVID-19)

(2020) Joungha Won et al.   Experimental Neurobiology

Sensitivity of different RT-qPCR solutions for SARS-CoV-2 detection

(2020) Julia Alcoba-Florez et al.   INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES

A recurrent mutation at position 26,340 of SARS-CoV-2 is associated with failure of the E-gene qRT-PCR utilized in a commercial dual-target diagnostic assay

(2020) Maria Artesi et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Comparison of seven commercial RT-PCR diagnostic kits for COVID-19

(2020) Puck B. van Kasteren et al.   JOURNAL OF CLINICAL VIROLOGY

COVID-19 diagnostics in context

(2020) Ralph Weissleder et al.   Science Translational Medicine

Pitfalls in SARS‐CoV‐2 PCR diagnostics

(2020) Kerstin Wernike et al.   Transboundary and Emerging Diseases

Comparative analysis of primer-probe sets for RT-qPCR of COVID-19 causative virus (SARS-CoV-2)

(2020) Yujin Jung et al.   ACS Infectious Diseases

Presence of mismatches between diagnostic PCR assays and coronavirus SARS-CoV-2 genome

(2020) Kashif Aziz Khan et al.   Royal Society Open Science

Analytical sensitivity and efficiency comparisons of SARS-CoV-2 RT–qPCR primer–probe sets

(2020) Chantal B. F. Vogels et al.   Nature Microbiology

Performance Assessment of SARS-CoV-2 PCR Assays Developed by WHO Referral Laboratories

(2020) Sibyle Etievant et al.   Journal of Clinical Medicine

Cautionary Note on Contamination of Reagents Used for Molecular Detection of SARS-CoV-2

(2020) Jim F Huggett et al.   CLINICAL CHEMISTRY

Genetics and genomics of SARS-CoV-2: A review of the literature with the special focus on genetic diversity and SARS-CoV-2 genome detection

(2020) Azadeh Rahimi et al.   GENOMICS

Double-quencher probes improve detection sensitivity toward Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in a reverse-transcription polymerase chain reaction (RT-PCR) assay

(2020) Yosuke Hirotsu et al.   JOURNAL OF VIROLOGICAL METHODS

The coronavirus is mutating — does it matter?

(2020) Ewen Callaway   NATURE

Considerations for diagnostic COVID-19 tests

(2020) Olivier Vandenberg et al.   NATURE REVIEWS MICROBIOLOGY

Adjusting RT-qPCR conditions to avoid unspecific amplification in SARS-CoV-2 diagnosis

(2020) Lauren Hubert Jaeger et al.   INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES

Variable sensitivity in molecular detection of SARS-CoV-2 in European Expert Laboratories: External Quality Assessment, June – July 2020

(2020) Carlo Fischer et al.   JOURNAL OF CLINICAL MICROBIOLOGY

Parallelization of MAFFT for large-scale multiple sequence alignments

(2018) Tsukasa Nakamura et al.   BIOINFORMATICS

Nextstrain: real-time tracking of pathogen evolution

(2018) James Hadfield et al.   BIOINFORMATICS

The evolution of seasonal influenza viruses

(2017) Velislava N. Petrova et al.   NATURE REVIEWS MICROBIOLOGY

Evaluation of TaqMan qPCR System Integrating Two Identically Labelled Hydrolysis Probes in Single Assay

(2017) Alexander Nagy et al.   Scientific Reports

RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies

(2014) Alexandros Stamatakis   BIOINFORMATICS

A novel fluorescence-based assay for the rapid detection and quantification of cellular deoxyribonucleoside triphosphates

(2011) Peter M. Wilson et al.   NUCLEIC ACIDS RESEARCH

SeaView Version 4: A Multiplatform Graphical User Interface for Sequence Alignment and Phylogenetic Tree Building

(2009) M. Gouy et al.   MOLECULAR BIOLOGY AND EVOLUTION