Article
Virology
Yakup Artik, Alp B. Cosgun, Nevra P. Cesur, Nedret Hizel, Yavuz Uyar, Haydar Sur, Alp Ayan
Summary: The present study evaluates the effectiveness of LAMP method for rapid screening diagnostic test of COVID-19 and compares it with the gold standard q-RT-PCR. The results show that LAMP method performs similarly to q-RT-PCR in some cases and offers the advantages of speed and cost-effectiveness.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Asmaa M. Altamimi, Dalia A. Obeid, Taghreed A. Alaifan, Moroje T. Taha, Marwa T. Alhothali, Fahad A. Alzahrani, Ahmad M. Albarrag
Summary: The study focuses on the clinical and analytical performance of SARS-CoV-2 (RT-PCR) commercial kits, with four kits showing a sensitivity of 100% and all tested kits scoring above 95% in specificity. Most of the commercially available RT-PCR kits in the study can be used for routine diagnosis of patients with SARS-CoV-2.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Virology
Carolina Hernandez, Carolina Florez, Sergio Castaneda, Nathalia Ballesteros, David Martinez, Adriana Castillo, Marina Munoz, Sergio Gomez, Angelica Rico, Liseth Pardo, Alberto Paniz-Mondolfi, Juan David Ramirez
Summary: The study evaluated the diagnostic performance of nine commercial reverse-transcription polymerase chain reaction kits for SARS-CoV-2 testing, showing optimal performance and high discriminatory power. Variability in sensitivity, specificity, and E gene Ct Values was observed among the kits, potentially influenced by genetic variability of the virus.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Public, Environmental & Occupational Health
Sanchita Roy Pradhan, M. H. Yashavarddhan, Ashish Gupta, Pramod Kumar, Anuj Kumar, Nazneen Arif, Usha Agrawal, R. Suresh Kumar, Shalini Singh
Summary: The Indian Council of Medical Research (ICMR) established a high-throughput viral diagnostic laboratory (HTVDL) during the COVID-19 pandemic to expand testing capacity and adopt rapid testing procedures. The HTVDL provided testing support in two regions of India with a capacity of 6000 tests per day. This article shares the experience of establishing a high-throughput laboratory in developing countries like India and is helpful globally for setting up HTVDLs.
FRONTIERS IN PUBLIC HEALTH
(2023)
Article
Microbiology
Rifat Tasnim Juthi, Saiful Arefeen Sazed, Md Fahad Zamil, Mohammad Shafiul Alam
Summary: This study evaluated and compared the performance of three commercially available COVID-19 RT-PCR kits, with the Aridia (R) COVID-19 Real-Time PCR Test showing the best performance among them. However, the Novel Coronavirus (2019-nCoV) Nucleic Acid Detection Kit performed the worst among the three kits.
Article
Biochemical Research Methods
Ozlem Akgun Dogan, Betsi Kose, Nihat Bugra Agaoglu, Jale Yildiz, Gizem Alkurt, Yasemin Kendir Demirkol, Arzu Irvem, Gizem Dinler Doganay, Levent Doganay
Summary: A study compared RT-PCR results in nasopharyngeal, oro-nasopharyngeal, and saliva samples of COVID-19 patients, finding that saliva sampling did not increase test sensitivity in early infection, but viral RNA detection rates in saliva were similar to other samples by the 5th day.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Virology
Selen Zeliha Mart Komurcu, Yakup Artik, Nevra Pelin Cesur, Arzu Tanriverdi, Derya Cakir Erdogan, Sule Celik, Elif Yilmaz Gulec
Summary: Rapid and reliable detection of SARS-CoV-2 mutations is crucial in controlling virus transmission. Study found N501Y mutation in positive patients, providing important information for public health efforts.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Construction & Building Technology
Jostein Gohli, Ane Marie Anderson, Arne Broch Brantsaeter, Kari Oline Boifot, Carola Grub, Cathrine Lund Hadley, Andreas Lind, Ellen Susanne Pettersen, Arne Vasli Lund Soraas, Marius Dybwad
Summary: In this study, the quantitative analysis of SARS-CoV-2 in the air surrounding infected individuals with mild symptoms was conducted. The results showed a significant correlation between viral concentration in the air samples collected at one-meter distance with viral loads in the upper airways, mild coughing, and fever. The findings have potential implications for SARS-CoV-2 detection, monitoring, and understanding of transmission dynamics.
Article
Medicine, General & Internal
Nahal Eshghifar, Ali Busheri, Rojeet Shrestha, Safedin Beqaj
Summary: This study evaluated the analytical performance of rapid antigen tests used for detecting SARS-CoV-2 viral antigen compared to RT-PCR molecular assay. Results showed that rapid antigen tests are less sensitive than RT-PCR tests and are not reliable for asymptomatic patients with low viral load.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2021)
Article
Microbiology
Ranran Cao, Lirong Bao, Ming Pan, Cheng Zhang, Hongyu Liao, Li Liu, Yan Li, Mingyuan Li
Summary: This study analyzed the detection rates of SARS-CoV-2 in COVID-19 patients' fecal samples using four real-time RT-PCR kits and two pretreatment methods. The results showed that the Trizol pretreatment group had a slightly higher detection rate, and the DAAN kit had the highest NAT positive rate, which was related to the severity of the disease.
Article
Multidisciplinary Sciences
Marco Favaro, Walter Mattina, Enrico Salvatore Pistoia, Roberta Gaziano, Paolo Di Francesco, Simon Middleton, Silvia D'Angelo, Tullio Altarozzi, Carla Fontana
Summary: A new qualitative RT-PCR probe has been developed by the research team for detecting COVID-19 infections, with a coincidence rate of 0.96 and LoD of 140cp/reaction for N and 14cp/reaction for RdRp and E. The kit is designed to be open and can be distributed in large quantities to any microbiological laboratory worldwide.
SCIENTIFIC REPORTS
(2021)
Article
Virology
Noah Reich, Christopher F. Lowe, David Puddicombe, Nancy Matic, Jesse Greiner, Janet Simons, Victor Leung, Terry Chu, Hiten Naik, Nick Myles, Laura Burns, Marc G. Romney, Gordon Ritchie, Sylvie Champagne, Kent Dooley, Inna Sekirov, Aleksandra Stefanovic
Summary: The study shows that the discordance between initial NP PCR and repeat NP sampling PCR and/or serology is a rare occurrence, with only 5 cases (3.1%) reported.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Medical Laboratory Technology
Mei Yang, Shuang Cao, Yong Liu, Zhijie Zhang, Rui Zheng, Yuzhong Li, Jie Zhou, Chengguo Zong, Desheng Cao, Xiaosong Qin
Summary: This study evaluated the performance of five commercial RT-qPCR diagnostic kits for SARS-CoV-2 used in China. The results showed that all kits passed the performance verification, except for one. Da An and Liferiver demonstrated excellent clinical diagnostic specificity and sensitivity. This study can guide the selection or optimization of RT-qPCR diagnostic test kits for SARS-CoV-2.
CLINICA CHIMICA ACTA
(2022)
Article
Infectious Diseases
Moris Sangineto, Fabio Arena, Rosella De Nittis, Rosanna Villani, Crescenzio Gallo, Gaetano Serviddio
Summary: This study investigated the temporal profile of SARS-CoV-2 viral load in the region of Capitanata, Apulia, Italy, and found that viral load progressively decreased along the outbreak course; during the first epidemic peak, patients over 80 years old had significantly higher viral loads than younger subjects, but this age difference disappeared in May; it is suggested that there may be an attenuation of viral transmission or pathogenicity during the epidemic course.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Microbiology
Riffat Munir, Lesley Erica Scott, Lara Dominique Noble, Kim Steegen, Lucia Hans, Wendy Susan Stevens
Summary: Laboratory diagnosis is crucial in controlling infection transmission and reducing delays in clinical and public health responses. Alternatives to the current standard of care assays for SARS-CoV-2 are important due to global demands and supply shortages. This study evaluated the analytical performance of four molecular assays designed for SARS-CoV-2 detection, and found them to be suitable alternatives to the current standard of care assays.
MICROBIOLOGY SPECTRUM
(2023)
