Article
Plant Sciences
Maria Cecilia Terrile, Nuria Malena Tebez, Silvana Lorena Colman, Julieta Lisa Mateos, Esperanza Morato-Lopez, Nuria Sanchez-Lopez, Alicia Izquierdo-Alvarez, Anabel Marina, Luz Irina A. Calderon Villalobos, Mark Estelle, Antonio Martinez-Ruiz, Diego Fernando Fiol, Claudia Anahi Casalongue, Maria Jose Iglesias
Summary: E3 ubiquitin ligases are crucial in plant biology and play a key role in the perception and signaling of hormones. The redox modification of proteins through S-nitrosation serves as an important regulatory mechanism in hormonal signaling pathways. This study confirmed the S-nitrosation of TIR1 and COI1, highlighting the functional relevance of this modification in the response to auxins and jasmonates.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Albert W. Hinman, Hsin-Yi Yeh, Baptiste Roelens, Kei Yamaya, Alexander Woglar, Henri-Marc G. Bourbon, Peter Chi, Anne M. Villeneuve
Summary: Meiotic recombination plays essential roles in genetic diversity and genome inheritance, with DSB-3 identified as a protein promoting DSB formation in the nematode Caenorhabditis elegans. DSB-3 is interdependent with other DSB proteins and its localization supports its role as a homolog of MEI4 in conserved DSB-promoting complexes. Variations in meiotic programs across diverse organisms may contribute to the diversification of essential meiotic machinery components.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Hamida Laroussi, Ariadna B. Juarez-Martinez, Aline Le Roy, Elisabetta Boeri Erba, Frank Gabel, Bernard de Massy, Jan Kadlec
Summary: Meiotic recombination in mice is initiated by DNA double-strand breaks (DSBs), catalyzed by the TOPOVIL complex. This complex's activity is regulated by meiotic factors such as REC114, MEI4, and IHO1, but the mechanism is not well understood. This study reveals the molecular details of these factors and their interactions, including REC114 forming homodimers, associating with MEI4 as a heterotrimer, and IHO1 forming tetramers. The study also shows that IHO1 directly interacts with REC114 and shares a binding surface with TOPOVIBL and another meiotic factor ANKRD31. These findings suggest the existence of a ternary IHO1-REC114-MEI4 complex and highlight the potential regulatory role of REC114 in mediating interactions with multiple partners.
Editorial Material
Biochemistry & Molecular Biology
Yuan Xie, Minglei Zhao
Summary: Proper regulation of protein degradation is crucial for cell physiology. In the recent issue of Cell, Baek et al. revealed the assembly and disassembly mechanism of a widely present ubiquitin ligase class called CRL, through a key regulatory protein, CAND1.
Article
Multidisciplinary Sciences
Jie Li, Gregory J. Krause, Qi Gui, Susmita Kaushik, Gergely Rona, Qingyue Zhang, Feng-Xia Liang, Avantika Dhabaria, Carlos Anerillas, Jennifer L. Martindale, Nikita Vasilyev, Manor Askenazi, Beatrix Ueberheide, Evgeny Nudler, Myriam Gorospe, Ana Maria Cuervo, Michele Pagano
Summary: This study identifies a new function for mammalian SKP1 in the endolysosomes, which is independent of its established role as a component of ubiquitin ligases. SKP1 is found to be phosphorylated under nutrient-poor conditions, promoting V-ATPase assembly and acidification of late endosomes for degradation. Under nutrient-rich conditions, phosphorylated SKP1 is SUMOylated and allows unconventional secretion through interaction with SEC22B.
Article
Biochemistry & Molecular Biology
Orsolya Bilkei-Gorzo, Tiaan Heunis, Jose Luis Marin-Rubio, Francesca Romana Cianfanelli, Benjamin Bernard Armando Raymond, Joseph Inns, Daniela Fabrikova, Julien Peltier, Fiona Oakley, Ralf Schmid, Anetta Hartlova, Matthias Trost
Summary: This study reveals the importance of phagosomal ubiquitylation and the E3 ubiquitin ligase RNF115 in regulating innate immune functions during bacterial infections.
Review
Cell Biology
Corinne Grey, Bernard de Massy
Summary: The axial element plays a crucial role in establishing sister chromatid cohesion and meiotic recombination during prophase I of meiosis, contributing to the successful outcome of meiosis I.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Multidisciplinary Sciences
Dominic Johnson, Margaret Crawford, Tim Cooper, Corentin Claeys Bouuaert, Scott Keeney, Bertrand Llorente, Valerie Garcia, Matthew J. Neale
Summary: Genetic recombination during meiosis involves the repair of DNA double-strand breaks (DSBs) created by Spo11, with recent findings indicating the existence of multiple Spo11 DSBs termed 'double cuts'. These double cuts vary in length with a periodicity of 10.5 base pairs and play a role in recombination and DNA gap repair during meiosis.
Article
Biochemistry & Molecular Biology
Hyun Mi Kang, Dae Hun Kim, Mijin Kim, Yoohong Min, Bohyeon Jeong, Kyung Hee Noh, Da Yong Lee, Hyun-Soo Cho, Nam-Soon Kim, Cho-Rok Jung, Jung Hwa Lim
Summary: This study discovered that the FBXL17-SPAST pathway is involved in the pathogenicity of hereditary spastic paraplegia (HSP) through loss of function and quantitative regulation. Targeting FBXL17 could provide new insights into HSP therapeutics.
CELL AND BIOSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Christine R. Langlois, Viola Beier, Ozge Karayel, Jakub Chrustowicz, Dawafuti Sherpa, Matthias Mann, Brenda A. Schulman
Summary: Cells remodel their proteomes to adjust to environmental metabolism demands. The GID E3 ligase regulates the transition from gluconeogenesis to glycolysis, while the Gid10 substrate receptor plays a role in responding to changes in temperature, osmolarity, and nutrient availability, regulating plasma membrane quality control.
Article
Cell Biology
Xu Zhou, Kailun Fang, Yanlei Liu, Weidong Li, Yingjin Tan, Jiaming Zhang, Xiaoxia Yu, Guoqiang Wang, Yanan Zhang, Yongliang Shang, Liangran Zhang, Charlie Degui Chen, Shunxin Wang
Summary: This study reveals the role of ZFP541 and KCTD19 in regulating the progression of male meiosis and fertility. Knockout of these genes leads to structural and recombination defects, and they activate gene expression by repressing transcription repressors. Additionally, ZFP541 and KCTD19 also play a role in directly modulating chromatin organization.
CELL PROLIFERATION
(2023)
Article
Biochemistry & Molecular Biology
Talia Hatkevich, Danny E. Miller, Carolyn A. Turcotte, Margaret C. Miller, Jeff Sekelsky
Summary: A novel backup repair mechanism has been identified in fruit fly meiosis, where DSB repair is initiated by homologous recombination but completed by nonhomologous end joining in the absence of the homologous chromosome. This precise repair process is proposed to be conserved and used to overcome challenges in normal meiosis.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Plant Sciences
Kim Osman, Uthman Algopishi, James D. Higgins, Ian R. Henderson, Keith J. Edwards, F. Chris H. Franklin, Eugenio Sanchez-Moran
Summary: Meiotic recombination in wheat exhibits biased distribution of crossovers towards distal regions of chromosomes, creating challenges for plant breeders. Recent advances in wheat genomics and genome engineering, along with well-developed cytogenetics, provide new opportunities for manipulating recombination and unlocking genetic variation in wheat. This study provides a cytogenetic framework for functional studies and ongoing initiatives to manipulate recombination in the wheat genome.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Corentin Claeys Bouuaert, Stephen Pu, Juncheng Wang, Cedric Oger, Dima Daccache, Wei Xie, Dinshaw J. Patel, Scott Keeney
Summary: This study elucidates how Saccharomyces cerevisiae RMM proteins self-assemble on chromosome axes to create centers of DSB activity. Protein-DNA interactions drive condensation and recruitment of Spo11 complexes, highlighting the multilayered control of Spo11 in chromosome segregation.
Article
Biochemistry & Molecular Biology
Amanda B. Chai, Richard Callaghan, Ingrid C. Gelissen
Summary: The study demonstrates that NEDD4-1 regulates the expression and activity of P-gp, thereby facilitating the clearance of Aβ peptides from the brain. This finding offers a potential approach for the treatment of Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Correction
Cell Biology
Manti Guha, Satish Srinivasan, Kip Guja, Edison Mejia, Miguel Garcia-Diaz, F. Brad Johnson, Gordon Ruthel, Brett A. Kaufman, Eric F. Rappaport, M. Rebecca Glineburg, Ji-Kang Fang, Andres J. Klein-Szanto, Hiroshi Nakagawa, Jeelan Basha, Tapas Kundu, Narayan G. Avadhani
Article
Microbiology
Ziying Han, Shantoshini Dash, Cari A. Sagum, Gordon Ruthel, Chaitanya K. Jaladanki, Corbett T. Berry, Michael P. Schwoerer, Nina M. Harty, Bruce D. Freedman, Mark T. Bedford, Hao Fan, Sachdev S. Sidhu, Marius Sudol, Olena Shtanko, Ronald N. Harty
Article
Biochemistry & Molecular Biology
Ziying Han, Gordon Ruthel, Shantoshini Dash, Corbett T. Berry, Bruce D. Freedman, Ronald N. Harty, Olena Shtanko
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Multidisciplinary Sciences
Yongjuan Guan, N. Adrian Leu, Jun Ma, Lukas Chmatal, Gordon Ruthel, Jordana C. Bloom, Michael A. Lampson, John C. Schimenti, Mengcheng Luo, P. Jeremy Wang
Article
Multidisciplinary Sciences
Prasanna M. Chandramouleeswaran, Manti Guha, Masataka Shimonosono, Kelly A. Whelan, Hisatsugu Maekawa, Uma M. Sachdeva, Gordon Ruthel, Sarmistha Mukherjee, Noah Engel, Michael Gonzalez, James Garifallou, Shinya Ohashi, Andres J. Klein-Szanto, Clementina A. Mesaros, Ian A. Blair, Renata Pellegrino da Silva, Hakon Hakonarson, Eishi Noguchi, Joseph A. Baur, Hiroshi Nakagawa
Article
Virology
Jingjing Liang, Gordon Ruthel, Cari A. Sagum, Mark T. Bedford, Sachdev S. Sidhu, Marius Sudol, Chaitanya K. Jaladanki, Hao Fan, Bruce D. Freedman, Ronald N. Harty
Summary: The study identified the host WW-domain-containing protein WWOX as a novel interactor with VP40 and Z proteins, demonstrating that WWOX inhibits budding of VP40/Z VLPs and live virus in a PPxY/WW-domain-dependent manner. These findings expand the repertoire of host interactors regulating PPxY-mediated budding of RNA viruses and highlight competitive interplay and modular virus-host interactions impacting both the virus life cycle and the host cell.
JOURNAL OF VIROLOGY
(2021)
Article
Cell Biology
Laura M. McLane, Shin Foong Ngiow, Zeyu Chen, John Attanasio, Sasikanth Manne, Gordon Ruthel, Jennifer E. Wu, Ryan P. Staupe, Wei Xu, Ravi K. Amaravadi, Xiaowei Xu, Giorgos C. Karakousis, Tara C. Mitchell, Lynn M. Schuchter, Alexander C. Huang, Bruce D. Freedman, Michael R. Betts, E. John Wherry
Summary: The subcellular localization of T-bet and Eomes determines their regulatory activity in exhausted T cells, with Eomes having a dominant effect over T-bet. This mechanism reveals how these transcription factors regulate T cell exhaustion biology through their nuclear localization.
Article
Virology
Jingjing Liang, Gordon Ruthel, Bruce D. Freedman, Ronald N. Harty
Summary: Filoviruses (Ebola virus [EBOV] and Marburg virus [MARV]) are zoonotic, emerging pathogens that cause outbreaks of severe hemorrhagic fever in humans. A fundamental understanding of the virus-host interface is critical for understanding the biology of these viruses and for developing future strategies for therapeutic intervention.
JOURNAL OF VIROLOGY
(2022)
Article
Anatomy & Morphology
Ana Ripolles-Garcia, Gordon Ruthel, Gui-Shuang Ying, Yineng Chen, Nicolas Cuenca, Gustavo D. Aguirre, William A. Beltran
Summary: The study proposed a methodology for quantification of the canine retinal vasculature using OCTA, and validated this approach through comparison with other imaging methods. The results showed that OCTA had better resolution than traditional FA and could detect smaller vessels. Additionally, the study revealed the presence of different vascular plexuses in the canine retina.
FRONTIERS IN NEUROANATOMY
(2021)
Article
Cell Biology
Rong Liu, Seth D. Kasowitz, David Homolka, N. Adrian Leu, Jordan T. Shaked, Gordon Ruthel, Devanshi Jain, Huijuan Lin, Scott Keeney, Mengcheng Luo, Ramesh S. Pillai, P. Jeremy Wang
Summary: YTHDC2 plays a critical role in regulating the pachytene stage of meiosis, with its deficiency leading to cellular dysregulation and alterations in meiotic transcripts.
Article
Biochemistry & Molecular Biology
Swarna Bais, Abigail Norwillo, Gordon Ruthel, De'Broski R. Herbert, Bruce D. Freedman, Robert M. Greenberg
Summary: Schistosomiasis, caused by parasitic flatworms, is a neglected tropical disease. Current treatment options face challenges, highlighting the need for alternative targets. This study identifies the role of TRPML channels in parasite neuromuscular activity and tegumental integrity, making them potential drug targets for combating schistosomiasis.
Article
Immunology
Jessica M. Gilbertie, Thomas P. Schaer, Julie B. Engiles, Gabriela S. Seiler, Bennett L. Deddens, Alicia G. Schubert, Megan E. Jacob, Darko Stefanovski, Gordon Ruthel, Noreen J. Hickok, Devorah M. Stowe, Alexa Frink, Lauren V. Schnabel
Summary: The researchers developed BIO-PLY, a plasma-derived biologic with bactericidal activity against S. aureus biofilm aggregates. In an equine model of infectious arthritis, treatment with AMK+BIO-PLY resulted in reduced bacterial concentrations, decreased inflammation, and protected cartilage from proteoglycan loss.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Oncology
Vaibhav Jain, Sandra L. Harper, Amanda M. Versace, Dylan Fingerman, Gregory Schuyler Brown, Monika Bhardwaj, Mary Ann S. Crissey, Aaron R. Goldman, Gordon Ruthel, Qin Liu, Aleksandra Zivkovic, Holgar Stark, Meenhard Herlyn, Phyllis A. Gimotty, David W. Speicher, Ravi K. Amaravadi
Summary: The inhibition of lysosomal autophagy (LAI) can enhance cancer therapy, but tumor regrowth is common. This study discovered that LAI induced lipid metabolism enzymes in cancer cells, leading to an increase in cholesterol, sphingolipids, and glycosphingolipids. Inhibition of cholesterol/sphingolipid metabolism proteins and targeting UDP-glucose ceramide glucosyltransferase (UGCG) enhanced LAI cytotoxicity. The combination of UGCG inhibition and LAI significantly inhibited tumor growth and improved survival.
Article
Biochemistry & Molecular Biology
Rajesh Angireddy, Anindya Roy Chowdhury, Jacek Zielonka, Gordon Ruthel, Balaraman Kalyanaraman, Narayan G. Avadhani
FREE RADICAL BIOLOGY AND MEDICINE
(2020)