Article
Environmental Sciences
Eugene P. Sokolov, Linda Adzigbli, Stephanie Markert, Amanda Bundgaard, Angela Fago, Doerte Becher, Claudia Hirschfeld, Inna M. Sokolova
Summary: The study found that the mitochondria of mussels exhibit increased respiration flux, reduced oxidative stress, and mitochondrial damage under H/R stress through intrinsic mechanisms such as reversible protein phosphorylation, contributing to their hypoxia-tolerant mitochondrial phenotype.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Olivier Lurette, Hala Guedouari, Jordan L. Morris, Rebeca Martin-Jimenez, Julie-Pier Robichaud, Genevieve Hamel-Cote, Mehtab Khan, Nicholas Dauphinee, Nicolas Pichaud, Julien Prudent, Etienne Hebert-Chatelain
Summary: The architecture of mitochondria is regulated by the tyrosine kinase Src, which affects mitochondrial morphology independently of mitochondrial size, cellular respiration, or ATP levels. This study highlights a novel function for Src in the control of mitochondrial dynamics.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Erika Fernandez-Vizarra, Sandra Lopez-Calcerrada, Luke E. Formosa, Rafael Perez-Perez, Shujing Ding, Ian M. Fearnley, Joaquin Arenas, Miguel A. Martin, Massimo Zeviani, Michael T. Ryan, Cristina Ugalde
Summary: The study of mitochondrial respiratory chain function in relation to its structural organization is essential for understanding eukaryotic cell metabolism. The complexome profiling technique has provided valuable information on the composition and assembly of MRC complexes, including larger supercomplexes and respirasomes. SCAFI plays a role in connecting individual MRC complexes III and IV, but is not the main player in respirasome formation as previously thought.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2021)
Article
Biochemistry & Molecular Biology
Maria Maldonado, Kaitlyn Madison Abe, James Anthony Letts
Summary: This study uses the structures of plant respiratory complexes to analyze the impact of RNA editing on amino acid changes, providing a molecular explanation for the phenotypes of RNA editing mutants. The research bridges the gap between sequence and phenotype and offers a framework for further analysis of RNA editing mutants in plant mitochondria and chloroplasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biology
Ahmed-Noor A. Agip, Injae Chung, Alvaro Sanchez-Martinez, Alexander J. Whitworth, Judy Hirst
Summary: Respiratory complex I in Drosophila melanogaster has a 43-subunit structure with high homology to the mammalian counterpart. It is in a mammalian-type active resting state, with a fully ordered and enclosed ubiquinone-binding site, but with subtle conformational changes. The deactive resting state observed in mammals is not seen, but minor states with a deactive-type pi-bulge in subunit ND6-TMH3 are present.
Article
Microbiology
Deyamira Matuz-Mares, Oscar Flores-Herrera, Guadalupe Guerra-Sanchez, Lucero Romero-Aguilar, Hector Vazquez-Meza, Genaro Matus-Ortega, Federico Martinez, Juan Pablo Pardo
Summary: This study investigated the organization of mitochondrial respiratory supercomplexes in U. maydis under diverse energy conditions. The supercomplexes were found to have consistent composition and distribution regardless of growth conditions, with no evidence of association between complex II and the alternative NADH dehydrogenases with other respiratory complexes.
Review
Plant Sciences
Anthony Gandin, Pierre Dizengremel, Yves Jolivet
Summary: Ozone as a secondary air pollutant can cause oxidative stress in plants by producing ROS, which have dual roles as signaling molecules and inducers of oxidative damage. Mitochondria play a crucial role in coping with ozone-induced ROS production and the mitochondrial fate involves three steps including initial responses, detoxifying processes, and accelerated senescence leading to programmed cell death.
PLANT PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biology
Sven Dennerlein, Sabine Poerschke, Silke Oeljeklaus, Cong Wang, Ricarda Richter-Dennerlein, Johannes Sattmann, Diana Bauermeister, Elisa Hanitsch, Stefan Stoldt, Thomas Langer, Stefan Jakobs, Bettina Warscheid, Peter Rehling
Summary: TMEM223 and SMIM4 were identified as ribosome-associated proteins involved in complex IV and III biogenesis, respectively. They stimulate mRNA translation and support early assembly steps, linking translation machinery to the biogenesis of OXPHOS complexes.
Article
Multidisciplinary Sciences
Catherine A. Makarewich, Amir Z. Munir, Svetlana Bezprozvannaya, Aaron M. Gibson, Soo Young Kim, Misty S. Martin-Sandoval, Thomas P. Mathews, Luke I. Szweda, Rhonda Bassel-Duby, Eric N. Olson
Summary: New evidence suggests that certain noncoding RNA molecules actually code for microproteins. By using comparative genomics, researchers identified a microprotein called Mtlbn that is abundantly expressed in the heart. Mtlbn localizes to the inner mitochondrial membrane and interacts with subunits of the electron transport chain and respiratory supercomplexes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biology
Margherita Protasoni, Jan-Willem Taanman
Summary: Mitochondrial defects are often the cause of neurological diseases. However, when skin cells are fed with glucose, they rely less on mitochondria to generate energy, making it difficult to study these diseases. In this study, researchers investigated the use of galactose and fructose as alternative sugars to feed skin cells, and found that they can stimulate mitochondria to generate energy. They recommend using fructose as the preferred sugar for studying mitochondrial defects in skin cells.
Article
Oncology
Arvinder Kapur, Pooja Mehta, Aaron D. Simmons, Spencer S. Ericksen, Geeta Mehta, Sean P. Palecek, Mildred Felder, Zach Stenerson, Amruta Nayak, Jose Maria Ayuso Dominguez, Manish Patankar, Lisa M. Barroilhet
Summary: The Warburg effect, characterized by high glucose uptake and lactate release, is a hallmark of most cancers. Atovaquone, a mitochondrial complex III inhibitor, has been found to slow ovarian cancer growth and inhibit the proliferation of cancer stem cells both in vitro and in vivo. The drug is able to inhibit oxygen consumption and ATP production, and it alters several metabolic pathways. These findings provide mechanistic insights and preclinical data to support further investigation of atovaquone as a potential therapy for gynecologic cancers.
Review
Cell Biology
Kunwar Somesh Vikramdeo, Sarabjeet Kour Sudan, Ajay P. Singh, Seema Singh, Santanu Dasgupta
Summary: Mitochondria play a crucial role in cellular energy production and signaling pathways, and alterations in respiratory complexes can lead to mitochondrial disorders and cancer development.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Christopher F. Bennett, Katherine E. O'Malley, Elizabeth A. Perry, Eduardo Balsa, Pedro Latorre-Muro, Christopher L. Riley, Chi Luo, Mark Jedrychowski, Steven P. Gygi, Pere Puigserver
Summary: The protein complexes of the mitochondrial electron transport chain exist in isolation and in higher order assemblies known as supercomplexes or respirasomes. In a study, it was found that inhibiting the de novo pyrimidine synthesis enzyme DHODH can increase respirasome assembly and activity, while bypassing this inhibition through uridine supplementation decreases respirasome assembly. These findings demonstrate a link between nucleotide pools, peroxisomes, mitochondrial phospholipids, and cell growth rates.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Zhexun Huang, Qiao Su, Wuguo Li, Hui Ren, Huiqiang Huang, Anxun Wang
Summary: Anlotinib demonstrates promising anticancer activity in oral squamous cell carcinoma by suppressing mitochondrial respiration, inducing redox imbalance through NOX5, and inhibiting the AKT/eIF2α pathway.
JOURNAL OF GENETICS AND GENOMICS
(2021)
Review
Cell Biology
Katarzyna Glombik, Jan Detka, Boguslawa Budziszewska
Summary: Hormones play a crucial role in regulating neuronal and synaptic plasticity, neurogenesis, and metabolic activity in the brain, ensuring proper communication between the brain and the body. The influence of hormones on energy metabolism and mitochondrial function in the brain has gained significant attention, as excess or deficiency of hormones is associated with cell damage and loss of function in mitochondria.
Article
Biochemistry & Molecular Biology
Maria Maldonado, Kaitlyn Madison Abe, James Anthony Letts
Summary: This study uses the structures of plant respiratory complexes to analyze the impact of RNA editing on amino acid changes, providing a molecular explanation for the phenotypes of RNA editing mutants. The research bridges the gap between sequence and phenotype and offers a framework for further analysis of RNA editing mutants in plant mitochondria and chloroplasts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Abhilash Padavannil, Maria G. Ayala-Hernandez, Eimy A. Castellanos-Silva, James A. Letts
Summary: This review explores the possible functions of accessory subunits in mitochondrial Complex I (CI) and proposes a framework of experimentally testable hypotheses. Accessory subunits play important roles in CI assembly and function, and amino acid substitutions in these subunits may lead to CI deficiencies.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Multidisciplinary Sciences
Long Zhou, Maria Maldonado, Abhilash Padavannil, Fei Guo, James A. Letts
Summary: This study reveals the functional and structural diversity of eukaryotic respiration through investigating the respiratory chain of the ciliate Tetrahymena thermophila. It expands our understanding of core metabolism by identifying divergence in key components of eukaryotic respiration.
Article
Multidisciplinary Sciences
Liufeng Dai, Baojie Wang, Ting Wang, Etienne H. Meyer, Valentin Kettel, Natalie Hoffmann, Heather E. McFarlane, Shalan Li, Xuna Wu, Kelsey L. Picard, Patrick Giavalisco, Staffan Persson, Yi Zhang
Summary: Energy is crucial for cellular functions, but how actin dynamics are regulated by ATP levels in plant cells is not well understood. This study reveals an important role for TORC1 in coordinating ATP homeostasis and actin dynamics in plant cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Plant Sciences
Helene Roehricht, Jonathan Przybyla-Toscano, Joachim Forner, Clement Boussardon, Olivier Keech, Nicolas Rouhier, Etienne H. Meyer
Summary: This study identified an atypical mitochondrial ferredoxin (mFDX-like) that plays a crucial role in the assembly of complex I and formation of complex I-containing supercomplexes.
Article
Biology
Abhilash Padavannil, Anjaneyulu Murari, Shauna-Kay Rhooms, Edward Owusu-Ansah, James A. Letts
Summary: Respiratory complex I plays a crucial role in bioenergetic metabolism and shows differences in behavior between Protostomia and Deuterostomia. The structure and conformational states of Drosophila melanogaster complex I provide insight into its functions and mechanisms in different organisms.
Article
Plant Sciences
Julia Smirnova, Justus Loerke, Gunnar Kleinau, Andrea Schmidt, Joerg Buerger, Etienne H. Meyer, Thorsten Mielke, Patrick Scheerer, Ralph Bock, Christian M. T. Spahn, Reimo Zoschke
Summary: Translation in plant cells occurs in three compartments, and this study focuses on the structure of the 80S ribosomes in the cytosol. By using cryo-electron microscopy, the researchers solved the structure of translating tobacco ribosomes and gained insights into the molecular underpinnings of cytosolic translation in plants.
Correction
Plant Sciences
M. Maldonado, Z. Fan, K. M. Abe, J. A. Letts
Article
Plant Sciences
M. Maldonado, Z. Fan, K. M. Abe, J. A. Letts
Summary: The last steps of cellular respiration in plants are carried out by mitochondrial oxidative phosphorylation, which involves multi-subunit membrane protein complexes known as supercomplexes. However, the functions and structures of these supercomplexes remain largely unknown. In this study, the cryogenic electron microscopy structure of the supercomplex I + III2 from mung bean was determined, providing insights into the composition, differences, and behavior of complex I and supercomplex I + III2. Further studies are needed to understand the universal and clade-specific mechanisms of respiration.