Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning

Genetic engineering in organoids

(2021) Isaree Teriyapirom et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

New Insights and Enhanced Human Norovirus Cultivation in Human Intestinal Enteroids

(2021) Khalil Ettayebi et al.   mSphere

The Organoid Platform: Promises and Challenges as Tools in the Fight against COVID-19

(2021) Maarten H. Geurts et al.   Stem Cell Reports

Fast and efficient generation of knock-in human organoids using homology-independent CRISPR–Cas9 precision genome editing

(2020) Benedetta Artegiani et al.   NATURE CELL BIOLOGY

Bile acids and ceramide overcome the entry restriction for GII.3 human norovirus replication in human intestinal enteroids

(2020) Kosuke Murakami et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Universal and Efficient Electroporation Protocol for Genetic Engineering of Gastrointestinal Organoids

(2020) Anne-Marlen Gaebler et al.   Jove-Journal of Visualized Experiments

COVID-19: organoids go viral

(2020) Hans Clevers   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Genetic Manipulation of Human Intestinal Enteroids Demonstrates the Necessity of a Functional Fucosyltransferase 2 Gene for Secretor-Dependent Human Norovirus Infection

(2020) Kei Haga et al.   mBio

Stem cell‐derived enteroid cultures as a tool for dissecting host‐parasite interactions in the small intestinal epithelium

(2020) Miriam F. Hares et al.   PARASITE IMMUNOLOGY

Human norovirus exhibits strain-specific sensitivity to host interferon pathways in human intestinal enteroids

(2020) Shih-Ching Lin et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rotavirus induces intercellular calcium waves through ADP signaling

(2020) Alexandra L. Chang-Graham et al.   SCIENCE

L-WRN conditioned medium for gastrointestinal epithelial stem cell culture shows replicable batch-to-batch activity levels across multiple research teams

(2019) Kelli L. VanDussen et al.   Stem Cell Research

Human colon organoids reveal distinct physiologic and oncogenic Wnt responses

(2019) Birgitta E. Michels et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Drug Inducible CRISPR/Cas Systems

(2019) Jingfang Zhang et al.   Computational and Structural Biotechnology Journal

Novel Segment- and Host-Specific Patterns of Enteroaggregative Escherichia coli Adherence to Human Intestinal Enteroids

(2018) Anubama Rajan et al.   mBio

Modelling Cryptosporidium infection in human small intestinal and lung organoids

(2018) Inha Heo et al.   Nature Microbiology

Visualization and targeting of LGR5+ human colon cancer stem cells

(2017) Mariko Shimokawa et al.   NATURE

Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening

(2017) Julia Joung et al.   Nature Protocols

An analysis of possible off target effects following CAS9/CRISPR targeted deletions of neuropeptide gene enhancers from the mouse genome

(2017) Elizabeth Anne Hay et al.   NEUROPEPTIDES

A Protocol for Multiple Gene Knockout in Mouse Small Intestinal Organoids Using a CRISPR-concatemer

(2017) Alessandra Merenda et al.   Jove-Journal of Visualized Experiments

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Human mini-guts: new insights into intestinal physiology and host–pathogen interactions

(2016) Julie G. In et al.   Nature Reviews Gastroenterology & Hepatology

Replication of human noroviruses in stem cell-derived human enteroids

(2016) K. Ettayebi et al.   SCIENCE

Engineering Human Stem Cell Lines with Inducible Gene Knockout using CRISPR/Cas9

(2015) Yuejun Chen et al.   Cell Stem Cell

Human Enteroids/Colonoids and Intestinal Organoids Functionally Recapitulate Normal Intestinal Physiology and Pathophysiology

(2015) Nicholas C. Zachos et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Human Intestinal Enteroids: a New Model To Study Human Rotavirus Infection, Host Restriction, and Pathophysiology

(2015) Kapil Saxena et al.   JOURNAL OF VIROLOGY

Modeling colorectal cancer using CRISPR-Cas9–mediated engineering of human intestinal organoids

(2015) Mami Matano et al.   NATURE MEDICINE

Efficient genetic engineering of human intestinal organoids using electroporation

(2015) Masayuki Fujii et al.   Nature Protocols

Multiplex CRISPR/Cas9-based genome editing for correction of dystrophin mutations that cause Duchenne muscular dystrophy

(2015) David G. Ousterout et al.   Nature Communications

A Protocol for Lentiviral Transduction and Downstream Analysis of Intestinal Organoids

(2015) Jooske F. Van Lidth de Jeude et al.   Jove-Journal of Visualized Experiments

Human enteroids as an ex-vivo model of host–pathogen interactions in the gastrointestinal tract

(2014) Jennifer Foulke-Abel et al.   EXPERIMENTAL BIOLOGY AND MEDICINE

Universal Real-Time PCR-Based Assay for Lentiviral Titration

(2014) Wojciech Barczak et al.   MOLECULAR BIOTECHNOLOGY

The new frontier of genome engineering with CRISPR-Cas9

(2014) J. A. Doudna et al.   SCIENCE

Adult Stem Cells in the Small Intestine Are Intrinsically Programmed with Their Location-Specific Function

(2014) Sabine Middendorp et al.   STEM CELLS

Functional Repair of CFTR by CRISPR/Cas9 in Intestinal Stem Cell Organoids of Cystic Fibrosis Patients

(2013) Gerald Schwank et al.   Cell Stem Cell

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

In vitro expansion and genetic modification of gastrointestinal stem cells in spheroid culture

(2013) Hiroyuki Miyoshi et al.   Nature Protocols

Genome-Scale CRISPR-Cas9 Knockout Screening in Human Cells

(2013) Ophir Shalem et al.   SCIENCE

Genetic Screens in Human Cells Using the CRISPR-Cas9 System

(2013) Tim Wang et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Long-term Expansion of Epithelial Organoids From Human Colon, Adenoma, Adenocarcinoma, and Barrett's Epithelium

(2011) Toshiro Sato et al.   GASTROENTEROLOGY

Genome editing with engineered zinc finger nucleases

(2010) Fyodor D. Urnov et al.   NATURE REVIEWS GENETICS