An analysis of possible off target effects following CAS9/CRISPR targeted deletions of neuropeptide gene enhancers from the mouse genome

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Title
An analysis of possible off target effects following CAS9/CRISPR targeted deletions of neuropeptide gene enhancers from the mouse genome
Authors
Keywords
Neuropeptide, enhancer, CAS9/CRISPR, Off-target effects, Transgenic mouse, CAS9 mRNA, gRNA, 1-cell mouse embryo, Cytoplasmic microinjection, CRISPR design tool, UCSC browser BLAT, PCR, Sanger sequencing
Journal
NEUROPEPTIDES
Volume 64, Issue -, Pages 101-107
Publisher
Elsevier BV
Online
2016-11-04
DOI
10.1016/j.npep.2016.11.003

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