CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA

Human cleaving embryos enable efficient mitochondrial base-editing with DdCBE

(2022) Yinghui Wei et al.   Cell Discovery

TALEN outperforms Cas9 in editing heterochromatin target sites

(2021) Surbhi Jain et al.   Nature Communications

The potential of mitochondrial genome engineering

(2021) Pedro Silva-Pinheiro et al.   NATURE REVIEWS GENETICS

The TFAM-to-mtDNA ratio defines inner-cellular nucleoid populations with distinct activity levels

(2021) Christian Brüser et al.   Cell Reports

DdCBE mediates efficient and inheritable modifications in mouse mitochondrial genome

(2021) Jiayin Guo et al.   Molecular Therapy-Nucleic Acids

Evaluation and minimization of Cas9-independent off-target DNA editing by cytosine base editors

(2020) Jordan L. Doman et al.   NATURE BIOTECHNOLOGY

Comprehensive molecular characterization of mitochondrial genomes in human cancers

(2020) Yuan Yuan et al.   NATURE GENETICS

Therapeutic Manipulation of mtDNA Heteroplasmy: A Shifting Perspective

(2020) Christopher B. Jackson et al.   TRENDS IN MOLECULAR MEDICINE

A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing

(2020) Beverly Y. Mok et al.   NATURE

Extreme heterogeneity of human mitochondrial DNA from organelles to populations

(2020) James B. Stewart et al.   NATURE REVIEWS GENETICS

Phage-Assisted Evolution of Bacillus methanolicus Methanol Dehydrogenase 2

(2019) Timothy B. Roth et al.   ACS Synthetic Biology

CRISPResso2 provides accurate and rapid genome editing sequence analysis

(2019) Kendell Clement et al.   NATURE BIOTECHNOLOGY

Continuous evolution of base editors with expanded target compatibility and improved activity

(2019) Benjamin W. Thuronyi et al.   NATURE BIOTECHNOLOGY

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Early loss of mitochondrial complex I and rewiring of glutathione metabolism in renal oncocytoma

(2018) Raj K. Gopal et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mitochondrial Genome Engineering: The Revolution May Not Be CRISPR-Ized

(2018) Payam A. Gammage et al.   TRENDS IN GENETICS

Linear mitochondrial DNA is rapidly degraded by components of the replication machinery

(2018) Viktoriya Peeva et al.   Nature Communications

The mitochondrial DNA polymerase gamma degrades linear DNA fragments precluding the formation of deletions

(2018) Nadee Nissanka et al.   Nature Communications

An APOBEC3A-Cas9 base editor with minimized bystander and off-target activities

(2018) Jason M Gehrke et al.   NATURE BIOTECHNOLOGY

Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage

(2017) Nicole M. Gaudelli et al.   NATURE

Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance

(2016) Ahmed H. Badran et al.   NATURE

Programmable editing of a target base in genomic DNA without double-stranded DNA cleavage

(2016) Alexis C. Komor et al.   NATURE

SeqKit: A Cross-Platform and Ultrafast Toolkit for FASTA/Q File Manipulation

(2016) Wei Shen et al.   PLoS One

Continuous directed evolution of DNA-binding proteins to improve TALEN specificity

(2015) Basil P Hubbard et al.   NATURE METHODS

Direct observation of TALE protein dynamics reveals a two-state search mechanism

(2015) Luke Cuculis et al.   Nature Communications

Development of potent in vivo mutagenesis plasmids with broad mutational spectra

(2015) Ahmed H. Badran et al.   Nature Communications

Mitochondrially targeted ZFNs for selective degradation of pathogenic mitochondrial genomes bearing large-scale deletions or point mutations

(2014) P. A. Gammage et al.   EMBO Molecular Medicine

Negative selection and stringency modulation in phage-assisted continuous evolution

(2014) Jacob C Carlson et al.   Nature Chemical Biology

An online bioinformatics tool predicts zinc finger and TALE nuclease off-target cleavage

(2014) Eli J. Fine et al.   NUCLEIC ACIDS RESEARCH

Specific elimination of mutant mitochondrial genomes in patient-derived cells by mitoTALENs

(2013) Sandra R Bacman et al.   NATURE MEDICINE

Mitochondrial diseases

(2012) Anthony HV Schapira   LANCET

Mitochondrial disorders as windows into an ancient organelle

(2012) Scott B. Vafai et al.   NATURE

FLASH assembly of TALENs for high-throughput genome editing

(2012) Deepak Reyon et al.   NATURE BIOTECHNOLOGY

TAL effector RVD specificities and efficiencies

(2012) Jana Streubel et al.   NATURE BIOTECHNOLOGY

A system for the continuous directed evolution of biomolecules

(2011) Kevin M. Esvelt et al.   NATURE

Super-resolution microscopy reveals that mammalian mitochondrial nucleoids have a uniform size and frequently contain a single copy of mtDNA

(2011) C. Kukat et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA