Investigation of Tryptic Protein Digestion in Microdroplets and in Bulk Solution

Optimizing Coaxial Sonic Spray Geometry for Generating Water Microdroplets

(2022) Maria T. Dulay et al.   ANALYTICAL CHEMISTRY

Spontaneous Reduction-Induced Degradation of Viologen Compounds in Water Microdroplets and Its Inhibition by Host–Guest Complexation

(2022) Chu Gong et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Can electric fields drive chemistry for an aqueous microdroplet?

(2022) Hongxia Hao et al.   Nature Communications

Antibodies to watch in 2021

(2021) Hélène Kaplon et al.   mAbs

Microdroplet Ultrafast Reactions Speed Antibody Characterization

(2021) Pengyi Zhao et al.   ANALYTICAL CHEMISTRY

Enzyme-Catalyzed Hydrolysis of Lipids in Immiscible Microdroplets Studied by Contained-Electrospray Ionization

(2021) Benjamin J. Burris et al.   ANALYTICAL CHEMISTRY

Microdroplet Mass Spectrometry Enables Extremely Accelerated Pepsin Digestion of Proteins

(2021) Tobias Rainer et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

In-Source Microdroplet Derivatization Using Coaxial Contained-Electrospray Mass Spectrometry for Enhanced Sensitivity in Saccharide Analysis

(2021) Derik R. Heiss et al.   ANALYTICAL CHEMISTRY

Emerging microdroplet chemistry for synthesis and analysis

(2021) Xin Yan   INTERNATIONAL JOURNAL OF MASS SPECTROMETRY

Oxidation and Deamidation of Monoclonal Antibody Products: Potential Impact on Stability, Biological Activity, and Efficacy

(2021) Surbhi Gupta et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Ultrafast enzymatic digestion of proteins by microdroplet mass spectrometry

(2020) Xiaoqin Zhong et al.   Nature Communications

Accelerated Reaction Kinetics in Microdroplets: Overview and Recent Developments

(2020) Zhenwei Wei et al.   Annual Review of Physical Chemistry

Ultrasonic-Based Filter Aided Sample Preparation as the General Method to Sample Preparation in Proteomics

(2020) Luís B. Carvalho et al.   ANALYTICAL CHEMISTRY

Accelerating Electrochemical Reactions in a Voltage‐Controlled Interfacial Microreactor

(2020) Heyong Cheng et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Novel Combined Microstrip Resonator/Nanospray Ionization Source for Microwave-Assisted Trypsin Digestion of Proteins

(2020) Maria E. Rivera-Albarran et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Nonspecific cleavages arising from reconstitution of trypsin under mildly acidic conditions

(2020) Ben Niu et al.   PLoS One

Incorporating Electro-Epoxidation into Electrospray Ionization Mass Spectrometry for Simultaneous Analysis of Negatively and Positively Charged Unsaturated Glycerophospholipids

(2020) Shuli Tang et al.   JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY

Future perspectives of therapeutic monoclonal antibodies

(2019) Kanta Tsumoto et al.   Immunotherapy

Highly active enzyme–metal nanohybrids synthesized in protein–polymer conjugates

(2019) Xiaoyang Li et al.   Nature Catalysis

Influence of Inlet Capillary Temperature on the Microdroplet Chemistry Studied by Mass Spectrometry

(2019) Shibdas Banerjee et al.   JOURNAL OF PHYSICAL CHEMISTRY A

Development of an LC-MS/MS peptide mapping protocol for the NISTmAb

(2018) Trina Mouchahoir et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Enhancement of reaction rate in small-sized droplets: A combined analytical and simulation study

(2018) Sayantan Mondal et al.   JOURNAL OF CHEMICAL PHYSICS

Fluorescence Polarization Anisotropy in Microdroplets

(2018) Zhenpeng Zhou et al.   Journal of Physical Chemistry Letters

High throughput reaction screening using desorption electrospray ionization mass spectrometry

(2018) Michael Wleklinski et al.   Chemical Science

Non-Enzymatic and Site-Specific Glycan Shedding: A Novel Protein Degradation Pathway Observed in a Stabilized Form of RSV Prefusion F Protein

(2018) Jiang Qian et al.   ANALYTICAL CHEMISTRY

High yield accelerated reactions in nonvolatile microthin films: chemical derivatization for analysis of single-cell intracellular fluid

(2018) Zhenwei Wei et al.   Chemical Science

Extensive Chemical Modifications in the Primary Protein Structure of IgG1 Subvisible Particles Are Necessary for Breaking Immune Tolerance

(2017) Björn Boll et al.   MOLECULAR PHARMACEUTICS

Metallic Nanobrushes Made using Ambient Droplet Sprays

(2016) Depanjan Sarkar et al.   ADVANCED MATERIALS

Organic Reactions in Microdroplets: Reaction Acceleration Revealed by Mass Spectrometry

(2016) Xin Yan et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Analysis of monoclonal antibody oxidation by simple mixed mode chromatography

(2016) Jorge Alexander Pavon et al.   JOURNAL OF CHROMATOGRAPHY A

Polysorbates 20 and 80 Degradation by Group XV Lysosomal Phospholipase A 2 Isomer X1 in Monoclonal Antibody Formulations

(2016) Troii Hall et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Simultaneous monitoring of oxidation, deamidation, isomerization, and glycosylation of monoclonal antibodies by liquid chromatography-mass spectrometry method with ultrafast tryptic digestion

(2016) Yi Wang et al.   mAbs

Controlling nonspecific trypsin cleavages in LC-MS/MS-based shotgun proteomics using optimized experimental conditions

(2015) Pan Fang et al.   ANALYST

Anti–PD-1/PD-L1 therapy of human cancer: past, present, and future

(2015) Lieping Chen et al.   JOURNAL OF CLINICAL INVESTIGATION

Development of a quantitative mass spectrometry multi-attribute method for characterization, quality control testing and disposition of biologics

(2015) Richard S Rogers et al.   mAbs

Antibodies regulate antitumour immunity

(2015) Laurence Zitvogel et al.   NATURE

Microdroplet fusion mass spectrometry for fast reaction kinetics

(2015) Jae Kyoo Lee et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Accelerated Hantzsch electrospray synthesis with temporal control of reaction intermediates

(2015) Ryan M. Bain et al.   Chemical Science

Ester Hydrolysis of Polysorbate 80 in mAb Drug Product: Evidence in Support of the Hypothesized Risk After the Observation of Visible Particulate in mAb Formulations

(2014) Steven R. Labrenz   JOURNAL OF PHARMACEUTICAL SCIENCES

Structure-Based Prediction of Asparagine and Aspartate Degradation Sites in Antibody Variable Regions

(2014) Jasmin F. Sydow et al.   PLoS One

Oncology Meets Immunology: The Cancer-Immunity Cycle

(2013) Daniel S. Chen et al.   IMMUNITY

Effect of Water on the Chemical Stability of Amorphous Pharmaceuticals: I. Small Molecules

(2013) Satoshi Ohtake et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Improving Peptide Identification Sensitivity in Shotgun Proteomics by Stratification of Search Space

(2013) Gelio Alves et al.   JOURNAL OF PROTEOME RESEARCH

In-Source Fragmentation and the Sources of Partially Tryptic Peptides in Shotgun Proteomics

(2012) Jong-Seo Kim et al.   JOURNAL OF PROTEOME RESEARCH

Digestion and depletion of abundant proteins improves proteomic coverage

(2012) Bryan R Fonslow et al.   NATURE METHODS

Rapid Online Nonenzymatic Protein Digestion Combining Microwave Heating Acid Hydrolysis and Electrochemical Oxidation

(2010) Franco Basile et al.   ANALYTICAL CHEMISTRY

Identification and characterization of oxidation and deamidation sites in monoclonal rat/mouse hybrid antibodies

(2010) Vera Timm et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

A Quantitative Study of the Effects of Chaotropic Agents, Surfactants, and Solvents on the Digestion Efficiency of Human Plasma Proteins by Trypsin

(2010) Jennifer L. Proc et al.   JOURNAL OF PROTEOME RESEARCH

Stability of Protein Pharmaceuticals: An Update

(2010) Mark Cornell Manning et al.   PHARMACEUTICAL RESEARCH

Accelerated bimolecular reactions in microdroplets studied by desorption electrospray ionization mass spectrometry

(2010) Marion Girod et al.   Chemical Science

Impact of methionine oxidation on the binding of human IgG1 to FcRn and Fcγ receptors

(2009) Andrea Bertolotti-Ciarlet et al.   MOLECULAR IMMUNOLOGY

Enhanced Protein Digestion through the Confinement of Nanozeolite-Assembled Microchip Reactors

(2008) Ji et al.   ANALYTICAL CHEMISTRY

Heterogeneity of Monoclonal Antibodies Revealed by Charge-Sensitive Methods

(2008) J. Vlasak et al.   CURRENT PHARMACEUTICAL BIOTECHNOLOGY

Peptide mapping of therapeutic monoclonal antibodies: Improvements for increased speed and fewer artifacts

(2008) Lawrence W. Dick et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Post-translational Modifications of Recombinant Proteins: Significance for Biopharmaceuticals

(2008) Nigel Jenkins et al.   MOLECULAR BIOTECHNOLOGY

Heterogeneity of Monoclonal Antibodies

(2007) Hongcheng Liu et al.   JOURNAL OF PHARMACEUTICAL SCIENCES