Controlling nonspecific trypsin cleavages in LC-MS/MS-based shotgun proteomics using optimized experimental conditions

A protease inhibition strategy based on acceleration of autolysis

(2015) Yan Lv et al.   CHEMICAL COMMUNICATIONS

In-depth Characterization of the Cerebrospinal Fluid (CSF) Proteome Displayed Through the CSF Proteome Resource (CSF-PR)

(2014) Astrid Guldbrandsen et al.   MOLECULAR & CELLULAR PROTEOMICS

Why less is more when generating tryptic peptides in bottom-up proteomics

(2014) Siri Hildonen et al.   PROTEOMICS

Quantitative Bottom-Up Proteomics Depends on Digestion Conditions

(2013) Mark S. Lowenthal et al.   ANALYTICAL CHEMISTRY

N-linked glycoproteome profiling of human serum using tandem enrichment and multiple fraction concatenation

(2013) Cheng Ma et al.   ELECTROPHORESIS

Comprehensive Analysis of Protein Digestion Using Six Trypsins Reveals the Origin of Trypsin As a Significant Source of Variability in Proteomics

(2013) Scott J. Walmsley et al.   JOURNAL OF PROTEOME RESEARCH

Understanding the Role of Proteolytic Digestion on Discovery and Targeted Proteomic Measurements Using Liquid Chromatography Tandem Mass Spectrometry and Design of Experiments

(2013) Philip L. Loziuk et al.   JOURNAL OF PROTEOME RESEARCH

Improving Peptide Identification Sensitivity in Shotgun Proteomics by Stratification of Search Space

(2013) Gelio Alves et al.   JOURNAL OF PROTEOME RESEARCH

Cutting Edge Proteomics: Benchmarking of Six Commercial Trypsins

(2013) Jakob Bunkenborg et al.   JOURNAL OF PROTEOME RESEARCH

Protein Digestion: An Overview of the Available Techniques and Recent Developments

(2013) Linda Switzar et al.   JOURNAL OF PROTEOME RESEARCH

In-Source Fragmentation and the Sources of Partially Tryptic Peptides in Shotgun Proteomics

(2012) Jong-Seo Kim et al.   JOURNAL OF PROTEOME RESEARCH

Data Analysis Strategy for Maximizing High-Confidence Protein Identifications in Complex Proteomes Such as Human Tumor Secretomes and Human Serum

(2011) Huan Wang et al.   JOURNAL OF PROTEOME RESEARCH

Microscale affinity purification of trypsin reduces background peptides in matrix-assisted laser desorption/ionization mass spectrometry of protein digests

(2011) Ivo Chamrád et al.   Journal of Proteomics

Systematic and quantitative comparison of digest efficiency and specificity reveals the impact of trypsin quality on MS-based proteomics

(2011) Julia Maria Burkhart et al.   Journal of Proteomics

Taking Advantage of Nonspecific Trypsin Cleavages for the Identification of Seed Storage Proteins in Cereals

(2009) Kjell Sergeant et al.   JOURNAL OF PROTEOME RESEARCH

Improved visualization of protein consensus sequences by iceLogo

(2009) Niklaas Colaert et al.   NATURE METHODS