Purine biosynthetic enzymes assemble into liquid-like condensates dependent on the activity of chaperone protein HSP90

Oncogenic HSP90 facilitates metabolic alterations in aggressive B-cell lymphomas

(2021) M. Nieves Calvo-Vidal et al.   CANCER RESEARCH

Metabolic channeling: predictions, deductions, and evidence

(2021) Vidhi Pareek et al.   MOLECULAR CELL

Phase Separation and Cytotoxicity of Tau are Modulated by Protein Disulfide Isomerase and S-nitrosylation of this Molecular Chaperone

(2020) Kan Wang et al.   JOURNAL OF MOLECULAR BIOLOGY

DNA repair by Rad52 liquid droplets

(2020) Roxanne Oshidari et al.   Nature Communications

Hypoxia Drives the Assembly of the Multi-Enzyme Purinosome Complex

(2020) Cyrielle Doigneaux et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Hsp27 chaperones FUS phase separation under the modulation of stress-induced phosphorylation

(2020) Zhenying Liu et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Metabolomics and mass spectrometry imaging reveal channeled de novo purine synthesis in cells

(2020) Vidhi Pareek et al.   SCIENCE

Multiplexed Optical Sensors in Arrayed Islands of Cells for multimodal recordings of cellular physiology

(2020) Christopher A. Werley et al.   Nature Communications

Nwd1 Regulates Neuronal Differentiation and Migration through Purinosome Formation in the Developing Cerebral Cortex

(2020) Seiya Yamada et al.   iScience

Considerations and Challenges in Studying Liquid-Liquid Phase Separation and Biomolecular Condensates

(2019) Simon Alberti et al.   CELL

Regulation of Transmembrane Signaling by Phase Separation

(2019) Lindsay B. Case et al.   Annual Review of Biophysics

Phase separation of 53 BP 1 determines liquid‐like behavior of DNA repair compartments

(2019) Sinan Kilic et al.   EMBO JOURNAL

Intrinsic Disorder-Based Emergence in Cellular Biology: Physiological and Pathological Liquid-Liquid Phase Transitions in Cells

(2019) April L. Darling et al.   Polymers

RNA Granules Hitchhike on Lysosomes for Long-Distance Transport, Using Annexin A11 as a Molecular Tether

(2019) Ya-Cheng Liao et al.   CELL

Low-complexity domain of U1-70K modulates phase separation and aggregation through distinctive basic-acidic motifs

(2019) Song Xue et al.   Science Advances

Probing and engineering liquid-phase organelles

(2019) Dan Bracha et al.   NATURE BIOTECHNOLOGY

Mechanisms for Active Regulation of Biomolecular Condensates

(2019) Johannes Söding et al.   TRENDS IN CELL BIOLOGY

Relationship of Sequence and Phase Separation in Protein Low-Complexity Regions

(2018) Erik W. Martin et al.   BIOCHEMISTRY

Role of HSP90 in the Regulation of de Novo Purine Biosynthesis

(2018) Anthony M. Pedley et al.   BIOCHEMISTRY

FUS Phase Separation Is Modulated by a Molecular Chaperone and Methylation of Arginine Cation-π Interactions

(2018) Seema Qamar et al.   CELL

IUPred2A: context-dependent prediction of protein disorder as a function of redox state and protein binding

(2018) Bálint Mészáros et al.   NUCLEIC ACIDS RESEARCH

Different Material States of Pub1 Condensates Define Distinct Modes of Stress Adaptation and Recovery

(2018) Sonja Kroschwald et al.   Cell Reports

Kinase-controlled phase transition of membraneless organelles in mitosis

(2018) Arpan Kumar Rai et al.   NATURE

Microtubule-directed transport of purine metabolons drives their cytosolic transit to mitochondria

(2018) Chung Yu Chan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Biomolecular condensates: organizers of cellular biochemistry

(2017) Salman F. Banani et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Liquid behavior of cross-linked actin bundles

(2017) Kimberly L. Weirich et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

A New View into the Regulation of Purine Metabolism: The Purinosome

(2017) Anthony M. Pedley et al.   TRENDS IN BIOCHEMICAL SCIENCES

ATPase-Modulated Stress Granules Contain a Diverse Proteome and Substructure

(2016) Saumya Jain et al.   CELL

CRISPR-Cas9 induced mutations along de novo purine synthesis in HeLa cells result in accumulation of individual enzyme substrates and affect purinosome formation

(2016) Veronika Baresova et al.   MOLECULAR GENETICS AND METABOLISM

Spatial colocalization and functional link of purinosomes with mitochondria

(2016) J. B. French et al.   SCIENCE

A pH-driven transition of the cytoplasm from a fluid- to a solid-like state promotes entry into dormancy

(2016) Matthias Christoph Munder et al.   eLife

Dynamic Architecture of the Purinosome Involved in Human De Novo Purine Biosynthesis

(2015) Minjoung Kyoung et al.   BIOCHEMISTRY

Phase Transition of Spindle-Associated Protein Regulate Spindle Apparatus Assembly

(2015) Hao Jiang et al.   CELL

Quantitative Analysis of Purine Nucleotides Indicates That Purinosomes Increasede NovoPurine Biosynthesis

(2015) Hong Zhao et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

ClearVolume: open-source live 3D visualization for light-sheet microscopy

(2015) Loic A Royer et al.   NATURE METHODS

Purinosome formation as a function of the cell cycle

(2015) Chung Yu Chan et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Liquid demixing of intrinsically disordered proteins is seeded by poly(ADP-ribose)

(2015) Matthias Altmeyer et al.   Nature Communications

Axonal Transport of TDP-43 mRNA Granules Is Impaired by ALS-Causing Mutations

(2014) Nael H. Alami et al.   NEURON

Phase transitions of multivalent proteins can promote clustering of membrane receptors

(2014) Sudeep Banjade et al.   eLife

Dual Specificity Kinase DYRK3 Couples Stress Granule Condensation/Dissolution to mTORC1 Signaling

(2013) Frank Wippich et al.   CELL

Hsp70/Hsp90 chaperone machinery is involved in the assembly of the purinosome

(2013) J. B. French et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cell-free Formation of RNA Granules: Low Complexity Sequence Domains Form Dynamic Fibers within Hydrogels

(2012) Masato Kato et al.   CELL

Mapping Protein-Protein Proximity in the Purinosome

(2012) Yijun Deng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fiji: an open-source platform for biological-image analysis

(2012) Johannes Schindelin et al.   NATURE METHODS

Exploring the sequence determinants of amyloid structure using position-specific scoring matrices

(2010) Sebastian Maurer-Stroh et al.   NATURE METHODS

Reversible Compartmentalization of de Novo Purine Biosynthetic Complexes in Living Cells

(2008) S. An et al.   SCIENCE