CRISPR‐based point‐of‐care diagnostics incorporating Cas9, Cas12, and Cas13 enzymes advanced for SARS‐CoV‐2 detection

A Scalable, Easy-to-Deploy, Protocol for Cas13-Based Detection of SARS-CoV-2 Genetic Material

(2021) Jennifer N. Rauch et al.   JOURNAL OF CLINICAL MICROBIOLOGY

CRISPR/Cas technology as a promising weapon to combat viral infections

(2021) Carmen Escalona‐Noguero et al.   BIOESSAYS

Open-source RNA extraction and RT-qPCR methods for SARS-CoV-2 detection

(2021) Thomas G. W. Graham et al.   PLoS One

CRISPR-based detection of SARS-CoV-2: A review from sample to result

(2021) Reza Nouri et al.   BIOSENSORS & BIOELECTRONICS

Rapid and accurate nucleobase detection using FnCas9 and its application in COVID-19 diagnosis

(2021) Mohd. Azhar et al.   BIOSENSORS & BIOELECTRONICS

FnCas9-based CRISPR diagnostic for rapid and accurate detection of major SARS-CoV-2 variants on a paper strip

(2021) Manoj Kumar et al.   eLife

Virus against virus: a potential treatment for 2019-nCov (SARS-CoV-2) and other RNA viruses

(2020) Tuan M. Nguyen et al.   CELL RESEARCH

A new coronavirus associated with human respiratory disease in China

(2020) Fan Wu et al.   NATURE

A Novel Coronavirus Emerging in China — Key Questions for Impact Assessment

(2020) Vincent J. Munster et al.   NEW ENGLAND JOURNAL OF MEDICINE

U1 snRNP regulates cancer cell migration and invasion in vitro

(2020) Jung-Min Oh et al.   Nature Communications

Endogenous Type I CRISPR-Cas: From Foreign DNA Defense to Prokaryotic Engineering

(2020) Yanli Zheng et al.   Frontiers in Bioengineering and Biotechnology

Ultra-sensitive and high-throughput CRISPR-Powered COVID-19 diagnosis

(2020) Zhen Huang et al.   BIOSENSORS & BIOELECTRONICS

Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza

(2020) Timothy R. Abbott et al.   CELL

SARS-CoV-2 RNA polymerase as target for antiviral therapy

(2020) Luigi Buonaguro et al.   Journal of Translational Medicine

Massively multiplexed nucleic acid detection with Cas13

(2020) Cheri M. Ackerman et al.   NATURE

CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Nucleic Acid Detection Using CRISPR/Cas Biosensing Technologies

(2020) Rashid Aman et al.   ACS Synthetic Biology

The expanded development and application of CRISPR system for sensitive nucleotide detection

(2020) Fengjing Jia et al.   Protein & Cell

Rapid and sensitive detection of COVID-19 using CRISPR/Cas12a-based detection with naked eye readout, CRISPR/Cas12a-NER

(2020) Xinjie Wang et al.   Science Bulletin

Assay Techniques and Test Development for COVID-19 Diagnosis

(2020) Linda J. Carter et al.   ACS Central Science

Sequence-Specific Recognition of HIV-1 DNA with Solid-State CRISPR-Cas12a-Assisted Nanopores (SCAN)

(2020) Reza Nouri et al.   ACS Sensors

Distribution of SARS-CoV-2 PCR Cycle Threshold Values Provide Practical Insight Into Overall and Target-Specific Sensitivity Among Symptomatic Patients

(2020) Blake W Buchan et al.   AMERICAN JOURNAL OF CLINICAL PATHOLOGY

COVID-19 in allogeneic stem cell transplant: high false-negative probability and role of CRISPR and convalescent plasma

(2020) Alex Niu et al.   BONE MARROW TRANSPLANTATION

Pathophysiology, Transmission, Diagnosis, and Treatment of Coronavirus Disease 2019 (COVID-19)

(2020) W. Joost Wiersinga et al.   JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION

A Narrative Systematic Review of the Clinical Utility of Cycle Threshold Values in the Context of COVID-19

(2020) Sonia N. Rao et al.   Infectious Diseases and Therapy

Diagnostic methods and potential portable biosensors for coronavirus disease 2019

(2020) Feiyun Cui et al.   BIOSENSORS & BIOELECTRONICS

Pioneers of revolutionary CRISPR gene editing win chemistry Nobel

(2020) Heidi Ledford et al.   NATURE

Detection of SARS-CoV-2 with SHERLOCK One-Pot Testing

(2020) Julia Joung et al.   NEW ENGLAND JOURNAL OF MEDICINE

Development and evaluation of a rapid CRISPR-based diagnostic for COVID-19

(2020) Tieying Hou et al.   PLoS Pathogens

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

(2020) Maturada Patchsung et al.   Nature Biomedical Engineering

Streamlined inactivation, amplification, and Cas13-based detection of SARS-CoV-2

(2020) Jon Arizti-Sanz et al.   Nature Communications

Imaging Flies by Fluorescence Microscopy: Principles, Technologies, and Applications

(2019) Sebastian Dunst et al.   GENETICS

CRISPR/Cas Systems towards Next-Generation Biosensing

(2019) Yi Li et al.   TRENDS IN BIOTECHNOLOGY

Delivery Aspects of CRISPR/Cas for in Vivo Genome Editing

(2019) Danny Wilbie et al.   ACCOUNTS OF CHEMICAL RESEARCH

SHERLOCK: nucleic acid detection with CRISPR nucleases

(2019) Max J. Kellner et al.   Nature Protocols

HOLMESv2: A CRISPR-Cas12b-Assisted Platform for Nucleic Acid Detection and DNA Methylation Quantitation

(2019) Linxian Li et al.   ACS Synthetic Biology

Transcriptome Engineering with RNA-Targeting Type VI-D CRISPR Effectors

(2018) Silvana Konermann et al.   CELL

Delivering CRISPR: a review of the challenges and approaches

(2018) Christopher A. Lino et al.   DRUG DELIVERY

Polymerase chain reaction (PCR)-based methods: Promising molecular tools in dentistry

(2018) Shahriar Shahi et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

History of CRISPR-Cas from Encounter with a Mysterious Repeated Sequence to Genome Editing Technology

(2018) Yoshizumi Ishino et al.   JOURNAL OF BACTERIOLOGY

Cas13d Is a Compact RNA-Targeting Type VI CRISPR Effector Positively Modulated by a WYL-Domain-Containing Accessory Protein

(2018) Winston X. Yan et al.   MOLECULAR CELL

Field-deployable viral diagnostics using CRISPR-Cas13

(2018) Cameron Myhrvold et al.   SCIENCE

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Next-generation diagnostics with CRISPR

(2018) Daniel S. Chertow   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

Applications of gold nanoparticles in virus detection

(2018) Mohamed Shehata Draz et al.   Theranostics

Structural Basis for the RNA-Guided Ribonuclease Activity of CRISPR-Cas13d

(2018) Cheng Zhang et al.   CELL

RNA Targeting by Functionally Orthogonal Type VI-A CRISPR-Cas Enzymes

(2017) Alexandra East-Seletsky et al.   MOLECULAR CELL

RNA targeting with CRISPR–Cas13

(2017) Omar O. Abudayyeh et al.   NATURE

RNA editing with CRISPR-Cas13

(2017) David B. T. Cox et al.   SCIENCE

CRISPR-Cas: Adapting to change

(2017) Simon A. Jackson et al.   SCIENCE

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Endogenous RNase inhibitor contributes to stability of RNA in crude cell lysates: Applicability to RT-qPCR

(2016) Xiao Wang et al.   ANALYTICAL BIOCHEMISTRY

Structure and Engineering of Francisella novicida Cas9

(2016) Hisato Hirano et al.   CELL

Rapid, Low-Cost Detection of Zika Virus Using Programmable Biomolecular Components

(2016) Keith Pardee et al.   CELL

Two distinct RNase activities of CRISPR-C2c2 enable guide-RNA processing and RNA detection

(2016) Alexandra East-Seletsky et al.   NATURE

C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

(2016) Omar O. Abudayyeh et al.   SCIENCE

Major bacterial lineages are essentially devoid of CRISPR-Cas viral defence systems

(2016) David Burstein et al.   Nature Communications

Development and Evaluation of Reverse Transcription-Loop-Mediated Isothermal Amplification (RT-LAMP) Assay Coupled with a Portable Device for Rapid Diagnosis of Ebola Virus Disease in Guinea

(2016) Yohei Kurosaki et al.   PLoS Neglected Tropical Diseases

Ultrasensitive, rapid and inexpensive detection of DNA using paper based lateral flow assay

(2016) Miriam Jauset-Rubio et al.   Scientific Reports

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

Isothermal Amplification of Nucleic Acids

(2015) Yongxi Zhao et al.   CHEMICAL REVIEWS

Cas9 function and host genome sampling in Type II-A CRISPR–Cas adaptation

(2015) Yunzhou Wei et al.   GENES & DEVELOPMENT

Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

(2015) Sergey Shmakov et al.   MOLECULAR CELL

An updated evolutionary classification of CRISPR–Cas systems

(2015) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

CRISPR-Cas Systems: Prokaryotes Upgrade to Adaptive Immunity

(2014) Rodolphe Barrangou et al.   MOLECULAR CELL

Dumbbell-Shaped DNA Analytes Amplified by Polymerase Chain Reaction for Robust Single-Nucleotide Polymorphism Genotyping by Affinity Capillary Electrophoresis

(2013) Hideaki Shibata et al.   ANALYTICAL CHEMISTRY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Cas3 is a single-stranded DNA nuclease and ATP-dependent helicase in the CRISPR/Cas immune system

(2011) Tomas Sinkunas et al.   EMBO JOURNAL

CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III

(2011) Elitza Deltcheva et al.   NATURE

Interference by clustered regularly interspaced short palindromic repeat (CRISPR) RNA is governed by a seed sequence

(2011) E. Semenova et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Applications of Loop-Mediated Isothermal DNA Amplification

(2010) Shijun Fu et al.   APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

CRISPR interference: RNA-directed adaptive immunity in bacteria and archaea

(2010) Luciano A. Marraffini et al.   NATURE REVIEWS GENETICS

Chitosan-Coated Silica as a Solid Phase for RNA Purification in a Microfluidic Device

(2009) Kristin A. Hagan et al.   ANALYTICAL CHEMISTRY

Optimal Probe Length Varies for Targets with High Sequence Variation: Implications for Probe Library Design for Resequencing Highly Variable Genes

(2008) Niall J. Haslam et al.   PLoS One