Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 163, Issue 7, Pages 845-850Publisher
SPRINGER
DOI: 10.1007/s12010-010-9088-8
Keywords
Loop-mediated isothermal amplification; Pathogenic microorganisms; Detection; Application
Funding
- Shandong Science and Technology Development Project of People's Republic of China [2009GG10009001]
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During the last 10 years, with the development of loop-mediated isothermal amplification (LAMP) method, it has been widely applied in nucleic acid analysis because of its simplicity, rapidity, high efficiency, and outstanding specificity. This method employs a DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. Expensive equipment are not necessary to acquire a high level of precision, and there are fewer preparation steps compared to conventional PCR and real-time PCR assays. This paper briefly summarized the applications of LAMP method in pathogenic microorganisms, genetically modified ingredients, tumor detection, and embryo sex identification.
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