A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy
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Title
A genome-scale gain-of-function CRISPR screen in CD8 T cells identifies proline metabolism as a means to enhance CAR-T therapy
Authors
Keywords
CAR-T, PRODH2, T cell GOF screen, T cell CRISPR activation screen, metabolism, proline metabolism, dead-guide RNA, antitumor efficacy, mitochondria
Journal
Cell Metabolism
Volume 34, Issue 4, Pages 595-614.e14
Publisher
Elsevier BV
Online
2022-03-10
DOI
10.1016/j.cmet.2022.02.009
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Note: Only part of the references are listed.- Genome-wide CRISPR Screens in T Helper Cells Reveal Pervasive Crosstalk between Activation and Differentiation
- (2019) Johan Henriksson et al. CELL
- De novo design of potent and selective mimics of IL-2 and IL-15
- (2019) Daniel-Adriano Silva et al. NATURE
- Turning the corner on therapeutic cancer vaccines
- (2019) Robert E. Hollingsworth et al. npj Vaccines
- A safe and potent anti-CD19 CAR T cell therapy
- (2019) Zhitao Ying et al. NATURE MEDICINE
- The global pipeline of cell therapies for cancer
- (2019) Jia Xin Yu et al. NATURE REVIEWS DRUG DISCOVERY
- A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system
- (2019) Martin W. LaFleur et al. Nature Communications
- Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells
- (2019) Matthew B. Dong et al. CELL
- Proline oxidation fuels mitochondrial respiration during dark-induced leaf senescence in Arabidopsis thaliana
- (2019) Alban Launay et al. JOURNAL OF EXPERIMENTAL BOTANY
- In vivo CRISPR screening in CD8 T cells with AAV–Sleeping Beauty hybrid vectors identifies membrane targets for improving immunotherapy for glioblastoma
- (2019) Lupeng Ye et al. NATURE BIOTECHNOLOGY
- Enhanced CAR T cell expansion and prolonged persistence in pediatric patients with ALL treated with a low-affinity CD19 CAR
- (2019) Sara Ghorashian et al. NATURE MEDICINE
- Clinical lessons learned from the first leg of the CAR T cell journey
- (2019) Robbie G. Majzner et al. NATURE MEDICINE
- CRISPRa-mediated FOXP3 gene upregulation in mammalian cells
- (2019) Vida Forstnerič et al. Cell and Bioscience
- c-Jun overexpression in CAR T cells induces exhaustion resistance
- (2019) Rachel C. Lynn et al. NATURE
- Targeting REGNASE-1 programs long-lived effector T cells for cancer therapy
- (2019) Jun Wei et al. NATURE
- Unraveling the Complex Interplay Between T Cell Metabolism and Function
- (2018) Ramon I. Klein Geltink et al. Annual Review of Immunology
- Universal Chimeric Antigen Receptors for Multiplexed and Logical Control of T Cell Responses
- (2018) Jang Hwan Cho et al. CELL
- MetaboAnalyst 4.0: towards more transparent and integrative metabolomics analysis
- (2018) Jasmine Chong et al. NUCLEIC ACIDS RESEARCH
- CAR T cell immunotherapy for human cancer
- (2018) Carl H. June et al. SCIENCE
- Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function
- (2018) Eric Shifrut et al. CELL
- Impact of Genetic Polymorphisms on Human Immune Cell Gene Expression
- (2018) Benjamin J. Schmiedel et al. CELL
- Guide Swap enables genome-scale pooled CRISPR–Cas9 screening in human primary cells
- (2018) Pamela Y. Ting et al. NATURE METHODS
- Trends in the global immuno-oncology landscape
- (2018) Jun Tang et al. NATURE REVIEWS DRUG DISCOVERY
- Tisagenlecleucel in Adult Relapsed or Refractory Diffuse Large B-Cell Lymphoma
- (2018) Stephen J. Schuster et al. NEW ENGLAND JOURNAL OF MEDICINE
- Collagen prolyl 4-hydroxylase 1 is essential for HIF-1α stabilization and TNBC chemoresistance
- (2018) Gaofeng Xiong et al. Nature Communications
- Integrative Proteomics and Phosphoproteomics Profiling Reveals Dynamic Signaling Networks and Bioenergetics Pathways Underlying T Cell Activation
- (2017) Haiyan Tan et al. IMMUNITY
- Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection
- (2017) Justin Eyquem et al. NATURE
- Discovery of stimulation-responsive immune enhancers with CRISPR activation
- (2017) Dimitre R. Simeonov et al. NATURE
- Therapeutic T cell engineering
- (2017) Michel Sadelain et al. NATURE
- Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood
- (2017) Julia Joung et al. NATURE
- Enhancer connectome in primary human cells identifies target genes of disease-associated DNA elements
- (2017) Maxwell R Mumbach et al. NATURE GENETICS
- Differential analysis of RNA-seq incorporating quantification uncertainty
- (2017) Harold Pimentel et al. NATURE METHODS
- A subcellular map of the human proteome
- (2017) Peter J. Thul et al. SCIENCE
- Mitochondrial Dynamics Controls T Cell Fate through Metabolic Programming
- (2016) Michael D. Buck et al. CELL
- L-Arginine Modulates T Cell Metabolism and Enhances Survival and Anti-tumor Activity
- (2016) Roger Geiger et al. CELL
- Distinct Signaling of Coreceptors Regulates Specific Metabolism Pathways and Impacts Memory Development in CAR T Cells
- (2016) Omkar U. Kawalekar et al. IMMUNITY
- The Tumor Microenvironment Represses T Cell Mitochondrial Biogenesis to Drive Intratumoral T Cell Metabolic Insufficiency and Dysfunction
- (2016) Nicole E. Scharping et al. IMMUNITY
- Bioenergetic Insufficiencies Due to Metabolic Alterations Regulated by the Inhibitory Receptor PD-1 Are an Early Driver of CD8 + T Cell Exhaustion
- (2016) Bertram Bengsch et al. IMMUNITY
- Near-optimal probabilistic RNA-seq quantification
- (2016) Nicolas L Bray et al. NATURE BIOTECHNOLOGY
- Emerging concepts of T cell metabolism as a target of immunotherapy
- (2016) Chih-Hao Chang et al. NATURE IMMUNOLOGY
- A CRISPR-Based Toolbox for Studying T Cell Signal Transduction
- (2016) Shen Chi et al. Biomed Research International
- Proline dehydrogenase 2 (PRODH2) is a hydroxyproline dehydrogenase (HYPDH) and molecular target for treating primary hyperoxaluria
- (2015) Candice B. Summitt et al. BIOCHEMICAL JOURNAL
- Metabolic Competition in the Tumor Microenvironment Is a Driver of Cancer Progression
- (2015) Chih-Hao Chang et al. CELL
- Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex
- (2015) Silvana Konermann et al. NATURE
- Orthogonal gene knockout and activation with a catalytically active Cas9 nuclease
- (2015) James E Dahlman et al. NATURE BIOTECHNOLOGY
- Cas9 gRNA engineering for genome editing, activation and repression
- (2015) Samira Kiani et al. NATURE METHODS
- High-throughput functional genomics using CRISPR–Cas9
- (2015) Ophir Shalem et al. NATURE REVIEWS GENETICS
- MetaboAnalyst 3.0—making metabolomics more meaningful
- (2015) Jianguo Xia et al. NUCLEIC ACIDS RESEARCH
- Adoptive cell transfer as personalized immunotherapy for human cancer
- (2015) S. A. Rosenberg et al. SCIENCE
- Tissue-based map of the human proteome
- (2015) M. Uhlen et al. SCIENCE
- Prolyl-4-hydroxylase α subunit 2 promotes breast cancer progression and metastasis by regulating collagen deposition
- (2014) Gaofeng Xiong et al. BMC CANCER
- CRISPR-Cas9 Knockin Mice for Genome Editing and Cancer Modeling
- (2014) Randall J. Platt et al. CELL
- Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation
- (2014) Luke A. Gilbert et al. CELL
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- Molecular regulation of effector and memory T cell differentiation
- (2014) John T Chang et al. NATURE IMMUNOLOGY
- Metabolic Regulation of T Lymphocytes
- (2013) Nancie J. MacIver et al. Annual Review of Immunology
- Posttranscriptional Control of T Cell Effector Function by Aerobic Glycolysis
- (2013) Chih-Hao Chang et al. CELL
- The TNFRs OX40, 4-1BB, and CD40 as targets for cancer immunotherapy
- (2013) Amy E Moran et al. CURRENT OPINION IN IMMUNOLOGY
- Active STAT5 Regulates T-bet and Eomesodermin Expression in CD8 T Cells and Imprints a T-bet-Dependent Tc1 Program with Repressed IL-6/TGF- 1 Signaling
- (2013) M. Grange et al. JOURNAL OF IMMUNOLOGY
- The molecular basis of T cell acute lymphoblastic leukemia
- (2012) Pieter Van Vlierberghe et al. JOURNAL OF CLINICAL INVESTIGATION
- A positive/negative ion–switching, targeted mass spectrometry–based metabolomics platform for bodily fluids, cells, and fresh and fixed tissue
- (2012) Min Yuan et al. Nature Protocols
- Tumor Immunotherapy Directed at PD-1
- (2012) Antoni Ribas NEW ENGLAND JOURNAL OF MEDICINE
- Metabolism of [13C5]hydroxyproline in vitro and in vivo: implications for primary hyperoxaluria
- (2011) Juquan Jiang et al. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
- Activated STAT5 Promotes Long-Lived Cytotoxic CD8+ T Cells That Induce Regression of Autochthonous Melanoma
- (2011) M. Grange et al. CANCER RESEARCH
- Mitochondrial Respiratory Capacity Is a Critical Regulator of CD8+ T Cell Memory Development
- (2011) Gerritje J.W. van der Windt et al. IMMUNITY
- CD28 costimulation improves expansion and persistence of chimeric antigen receptor–modified T cells in lymphoma patients
- (2011) Barbara Savoldo et al. JOURNAL OF CLINICAL INVESTIGATION
- Proline Metabolism and Microenvironmental Stress
- (2010) James M. Phang et al. Annual Review of Nutrition
- Non-redundant functions of two proline dehydrogenase isoforms in Arabidopsis
- (2010) Dietmar Funck et al. BMC PLANT BIOLOGY
- Effectors and memories: Bcl-6 and Blimp-1 in T and B lymphocyte differentiation
- (2010) Shane Crotty et al. NATURE IMMUNOLOGY
- edgeR: a Bioconductor package for differential expression analysis of digital gene expression data
- (2009) M. D. Robinson et al. BIOINFORMATICS
- The primary hyperoxalurias
- (2009) Bernd Hoppe et al. KIDNEY INTERNATIONAL
- Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources
- (2009) Da Wei Huang et al. Nature Protocols
- Pscan: finding over-represented transcription factor binding site motifs in sequences from co-regulated or co-expressed genes
- (2009) Federico Zambelli et al. NUCLEIC ACIDS RESEARCH
- MetaboAnalyst: a web server for metabolomic data analysis and interpretation
- (2009) J. Xia et al. NUCLEIC ACIDS RESEARCH
- Increased protein intake on controlled oxalate diets does not increase urinary oxalate excretion
- (2009) John Knight et al. UROLOGICAL RESEARCH
- Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
- (2009) Ben Langmead et al. GENOME BIOLOGY
- A Novel Function for Hydroxyproline Oxidase in Apoptosis through Generation of Reactive Oxygen Species
- (2008) Sandra K. Cooper et al. JOURNAL OF BIOLOGICAL CHEMISTRY
- The Immunological Genome Project: networks of gene expression in immune cells
- (2008) Tracy S P Heng et al. NATURE IMMUNOLOGY
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