Circular RNAs: Characterization, cellular roles, and applications

Engineered circular ADAR-recruiting RNAs increase the efficiency and fidelity of RNA editing in vitro and in vivo

(2022) Zongyi Yi et al.   NATURE BIOTECHNOLOGY

Efficient in vitro and in vivo RNA editing via recruitment of endogenous ADARs using circular guide RNAs

(2022) Dhruva Katrekar et al.   NATURE BIOTECHNOLOGY

A bidirectional competitive interaction between circHomer1 and Homer1b within the orbitofrontal cortex regulates reversal learning

(2022) Alexander K. Hafez et al.   Cell Reports

circVAMP3 Drives CAPRIN1 Phase Separation and Inhibits Hepatocellular Carcinoma by Suppressing c‐Myc Translation

(2022) Shuai Chen et al.   Advanced Science

Circular RNA vaccines against SARS-CoV-2 and emerging variants

(2022) Liang Qu et al.   CELL

Circular RNA migration in agarose gel electrophoresis

(2022) Brian T. Abe et al.   MOLECULAR CELL

CRISPR/Cas13 effectors have differing extents of off-target effects that limit their utility in eukaryotic cells

(2022) Yuxi Ai et al.   NUCLEIC ACIDS RESEARCH

isoCirc catalogs full-length circular RNA isoforms in human transcriptomes

(2021) Ruijiao Xin et al.   Nature Communications

The RNA-binding protein SFPQ preserves long-intron splicing and regulates circRNA biogenesis in mammals

(2021) Lotte Victoria Winther Stagsted et al.   eLife

Engineering highly efficient backsplicing and translation of synthetic circRNAs

(2021) Rita M. Meganck et al.   Molecular Therapy-Nucleic Acids

mRNA Vaccines to Prevent COVID-19 Disease and Reported Allergic Reactions: Current Evidence and Suggested Approach

(2021) Aleena Banerji et al.   Journal of Allergy and Clinical Immunology-In Practice

Best practices to ensure robust investigation of circular RNAs: pitfalls and tips

(2021) Samantha Dodbele et al.   EMBO REPORTS

Mapping circular RNA structures in living cells by SHAPE-MaP

(2021) Si-Kun Guo et al.   METHODS

Signal and noise in circRNA translation

(2021) T.B. Hansen   METHODS

Comprehensive profiling of circular RNAs with nanopore sequencing and CIRI-long

(2021) Jinyang Zhang et al.   NATURE BIOTECHNOLOGY

Circular RNA-encoded oncogenic E-cadherin variant promotes glioblastoma tumorigenicity through activation of EGFR–STAT3 signalling

(2021) Xinya Gao et al.   NATURE CELL BIOLOGY

HNRNPL Circularizes ARHGAP35 to Produce an Oncogenic Protein

(2021) Yan Li et al.   Advanced Science

Structured elements drive extensive circular RNA translation

(2021) Chun-Kan Chen et al.   MOLECULAR CELL

NORAD-induced Pumilio phase separation is required for genome stability

(2021) Mahmoud M. Elguindy et al.   NATURE

Long noncoding RNA and protein abundance in lncRNPs

(2021) Man Wu et al.   RNA

lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription

(2021) Man Wu et al.   SCIENCE

Linking circular intronic RNA degradation and function in transcription by RNase H1

(2021) Xiang Li et al.   Science China-Life Sciences

Nanopore sequencing of brain-derived full-length circRNAs reveals circRNA-specific exon usage, intron retention and microexons

(2021) Karim Rahimi et al.   Nature Communications

Nanopore long-read sequencing of circRNAs

(2021) Karim Rahimi et al.   METHODS

Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling

(2021) Yang Gu et al.   Molecular Cancer

RNA circles with minimized immunogenicity as potent PKR inhibitors

(2021) Chu-Xiao Liu et al.   MOLECULAR CELL

Circular RNA ZNF609/CKAP5 mRNA interaction regulates microtubule dynamics and tumorigenicity

(2021) Francesca Rossi et al.   MOLECULAR CELL

Inhibition of SARS-CoV-2 coronavirus proliferation by designer antisense-circRNAs

(2021) Christina Pfafenrot et al.   NUCLEIC ACIDS RESEARCH

m6A-dependent biogenesis of circular RNAs in male germ cells

(2020) Chong Tang et al.   CELL RESEARCH

Biogenesis and Functions of Circular RNAs Come into Focus

(2020) Mei-Sheng Xiao et al.   TRENDS IN CELL BIOLOGY

A Highly Conserved Circular RNA Is Required to Keep Neural Cells in a Progenitor State in the Mammalian Brain

(2020) Christin Suenkel et al.   Cell Reports

Engineered Circular RNA Sponges Act as miRNA Inhibitors to Attenuate Pressure Overload-Induced Cardiac Hypertrophy

(2020) Annadoray Lavenniah et al.   MOLECULAR THERAPY

The expanding regulatory mechanisms and cellular functions of circular RNAs

(2020) Ling-Ling Chen   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Modulation of circRNA Metabolism by m6A Modification

(2020) Gaia Di Timoteo et al.   Cell Reports

An in vivo strategy for knockdown of circular RNAs

(2020) Nagarjuna Reddy Pamudurti et al.   Cell Discovery

A Quantitative Proteome Map of the Human Body

(2020) Lihua Jiang et al.   CELL

Targeting Mitochondria-Located circRNA SCAR Alleviates NASH via Reducing mROS Output

(2020) Qiyi Zhao et al.   CELL

CircZNF609 promotes cell proliferation, migration, invasion, and glycolysis in nasopharyngeal carcinoma through regulating HRAS via miR-338-3p

(2020) Zhonglu Liu et al.   MOLECULAR AND CELLULAR BIOCHEMISTRY

An inducible circular RNA circKcnt2 inhibits ILC3 activation to facilitate colitis resolution

(2020) Benyu Liu et al.   Nature Communications

Characterization of circular RNA transcriptomes in psoriasis and atopic dermatitis reveals disease‐specific expression profiles

(2020) Liviu I. Moldovan et al.   EXPERIMENTAL DERMATOLOGY

Screening for functional circular RNAs using the CRISPR–Cas13 system

(2020) Siqi Li et al.   NATURE METHODS

Gene regulation by long non-coding RNAs and its biological functions

(2020) Luisa Statello et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Safety and Efficacy of the BNT162b2 mRNA Covid-19 Vaccine

(2020) Fernando P. Polack et al.   NEW ENGLAND JOURNAL OF MEDICINE

Design and application of circular RNAs with protein-sponge function

(2020) Silke Schreiner et al.   NUCLEIC ACIDS RESEARCH

AUF1 ligand circPCNX reduces cell proliferation by competing with p21 mRNA to increase p21 production

(2020) Dimitrios Tsitsipatis et al.   NUCLEIC ACIDS RESEARCH

Circ-ZNF609 regulates G1-S progression in rhabdomyosarcoma

(2019) Francesca Rossi et al.   ONCOGENE

Allele-specific binding of RNA-binding proteins reveals functional genetic variants in the RNA

(2019) Ei-Wen Yang et al.   Nature Communications

RNA Circularization Diminishes Immunogenicity and Can Extend Translation Duration In Vivo

(2019) R. Alexander Wesselhoeft et al.   MOLECULAR CELL

The Landscape of Circular RNA in Cancer

(2019) Josh N. Vo et al.   CELL

Structure and Degradation of Circular RNAs Regulate PKR Activation in Innate Immunity

(2019) Chu-Xiao Liu et al.   CELL

Circular RNA F-circSR derived from SLC34A2-ROS1 fusion gene promotes cell migration in non-small cell lung cancer

(2019) Ke Wu et al.   Molecular Cancer

Highly efficient expression of circular RNA aptamers in cells using autocatalytic transcripts

(2019) Jacob L. Litke et al.   NATURE BIOTECHNOLOGY

The Translational Landscape of the Human Heart

(2019) Sebastiaan van Heesch et al.   CELL

CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress

(2019) Qidong Li et al.   Cell Metabolism

Intragenic antagonistic roles of protein and circRNA in tumorigenesis

(2019) Jlenia Guarnerio et al.   CELL RESEARCH

Loss of Super-Enhancer-Regulated circRNA Nfix Induces Cardiac Regeneration After Myocardial Infarction in Adult Mice

(2019) Senlin Huang et al.   CIRCULATION

RNA-based diagnostic and therapeutic strategies for cardiovascular disease

(2019) Dongchao Lu et al.   Nature Reviews Cardiology

An improved method for circular RNA purification using RNase R that efficiently removes linear RNAs containing G-quadruplexes or structured 3′ ends

(2019) Mei-Sheng Xiao et al.   NUCLEIC ACIDS RESEARCH

SplintQuant: A method for accurately quantifying circular RNA transcript abundance without reverse transcription bias

(2019) Vanessa M. Conn et al.   RNA

CircMYBL2, a circRNA from MYBL2, Regulates FLT3 Translation by Recruiting PTBP1 to Promote FLT3-ITD AML Progression

(2019) Yu-Meng Sun et al.   BLOOD

High-throughput RNA sequencing from paired lesional- and non-lesional skin reveals major alterations in the psoriasis circRNAome

(2019) Liviu-Ionut Moldovan et al.   BMC Medical Genomics

R Loops: From Physiological to Pathological Roles

(2019) Tatiana García-Muse et al.   CELL

N6-Methyladenosine Modification Controls Circular RNA Immunity

(2019) Y. Grace Chen et al.   MOLECULAR CELL

An atlas of cortical circular RNA expression in Alzheimer disease brains demonstrates clinical and pathological associations

(2019) Umber Dube et al.   NATURE NEUROSCIENCE

A Network of Noncoding Regulatory RNAs Acts in the Mammalian Brain

(2018) Benjamin Kleaveland et al.   CELL

Inborn Errors of RNA Lariat Metabolism in Humans with Brainstem Viral Infection

(2018) Shen-Ying Zhang et al.   CELL

RNA-Targeted Therapeutics

(2018) Stanley T. Crooke et al.   Cell Metabolism

Comprehensive circular RNA profiles in plasma reveals that circular RNAs can be used as novel biomarkers for systemic lupus erythematosus

(2018) Haixia Li et al.   CLINICA CHIMICA ACTA

A length-dependent evolutionarily conserved pathway controls nuclear export of circular RNAs

(2018) Chuan Huang et al.   GENES & DEVELOPMENT

A Circular RNA Protects Dormant Hematopoietic Stem Cells from DNA Sensor cGAS-Mediated Exhaustion

(2018) Pengyan Xia et al.   IMMUNITY

Circular RNA cSMARCA5 inhibits growth and metastasis in hepatocellular carcinoma

(2018) Jian Yu et al.   JOURNAL OF HEPATOLOGY

Computational Strategies for Exploring Circular RNAs

(2018) Yuan Gao et al.   TRENDS IN GENETICS

Engineering circular RNA for potent and stable translation in eukaryotic cells

(2018) R. Alexander Wesselhoeft et al.   Nature Communications

Enzyme-free digital counting of endogenous circular RNA molecules in B-cell malignancies

(2018) Mette Dahl et al.   LABORATORY INVESTIGATION

The Biogenesis, Functions, and Challenges of Circular RNAs

(2018) Xiang Li et al.   MOLECULAR CELL

Synthetic circular RNA functions as a miR-21 sponge to suppress gastric carcinoma cell proliferation

(2018) Xi Liu et al.   Molecular Therapy-Nucleic Acids

Tissue-Dependent Expression and Translation of Circular RNAs with Recombinant AAV Vectors In Vivo

(2018) Rita M. Meganck et al.   Molecular Therapy-Nucleic Acids

CIRCpedia v2: An Updated Database for Comprehensive Circular RNA Annotation and Expression Comparison

(2018) Rui Dong et al.   GENOMICS PROTEOMICS & BIOINFORMATICS

Plasma circular RNA profiling of patients with gastric cancer and their droplet digital RT-PCR detection

(2017) Tianwen Li et al.   JOURNAL OF MOLECULAR MEDICINE-JMM

Translation of CircRNAs

(2017) Nagarjuna Reddy Pamudurti et al.   MOLECULAR CELL

Circ-ZNF609 Is a Circular RNA that Can Be Translated and Functions in Myogenesis

(2017) Ivano Legnini et al.   MOLECULAR CELL

The Output of Protein-Coding Genes Shifts to Circular RNAs When the Pre-mRNA Processing Machinery Is Limiting

(2017) Dongming Liang et al.   MOLECULAR CELL

Sensing Self and Foreign Circular RNAs by Intron Identity

(2017) Y. Grace Chen et al.   MOLECULAR CELL

Coordinated circRNA Biogenesis and Function with NF90/NF110 in Viral Infection

(2017) Xiang Li et al.   MOLECULAR CELL

DHX9 suppresses RNA processing defects originating from the Alu invasion of the human genome

(2017) Tuğçe Aktaş et al.   NATURE

High-purity circular RNA isolation method (RPAD) reveals vast collection of intronic circRNAs

(2017) Amaresh C. Panda et al.   NUCLEIC ACIDS RESEARCH

Identification of HuR target circular RNAs uncovers suppression of PABPN1 translation by CircPABPN1

(2017) Kotb Abdelmohsen et al.   RNA Biology

Loss of a mammalian circular RNA locus causes miRNA deregulation and affects brain function

(2017) Monika Piwecka et al.   SCIENCE

The circular RNA circBIRC6 participates in the molecular circuitry controlling human pluripotency

(2017) Chun-Ying Yu et al.   Nature Communications

FUS affects circular RNA expression in murine embryonic stem cell-derived motor neurons

(2017) Lorenzo Errichelli et al.   Nature Communications

ciRS-7 exonic sequence is embedded in a long non-coding RNA locus

(2017) Steven P. Barrett et al.   PLoS Genetics

Analysis of aptamer discovery and technology

(2017) Matthew R. Dunn et al.   Nature Reviews Chemistry

Oncogenic Role of Fusion-circRNAs Derived from Cancer-Associated Chromosomal Translocations

(2016) Jlenia Guarnerio et al.   CELL

Noncoding RNA NORAD Regulates Genomic Stability by Sequestering PUMILIO Proteins

(2016) Sungyul Lee et al.   CELL

Diverse alternative back-splicing and alternative splicing landscape of circular RNAs

(2016) Xiao-Ou Zhang et al.   GENOME RESEARCH

Robust transcriptome-wide discovery of RNA-binding protein binding sites with enhanced CLIP (eCLIP)

(2016) Eric L Van Nostrand et al.   NATURE METHODS

Foxo3 circular RNA retards cell cycle progression via forming ternary complexes with p21 and CDK2

(2016) William W. Du et al.   NUCLEIC ACIDS RESEARCH

Increased complexity of circRNA expression during species evolution

(2016) Rui Dong et al.   RNA Biology

Comprehensive identification of internal structure and alternative splicing events in circular RNAs

(2016) Yuan Gao et al.   Nature Communications

A conserved abundant cytoplasmic long noncoding RNA modulates repression by Pumilio proteins in human cells

(2016) Ailone Tichon et al.   Nature Communications

The Biogenesis of Nascent Circular RNAs

(2016) Yang Zhang et al.   Cell Reports

CircRNA-protein complexes: IMP3 protein component defines subfamily of circRNPs

(2016) Tim Schneider et al.   Scientific Reports

The RNA Binding Protein Quaking Regulates Formation of circRNAs

(2015) Simon J. Conn et al.   CELL

A Human Interactome in Three Quantitative Dimensions Organized by Stoichiometries and Abundances

(2015) Marco Y. Hein et al.   CELL

Circular RNA is enriched and stable in exosomes: a promising biomarker for cancer diagnosis

(2015) Yan Li et al.   CELL RESEARCH

In-Gel Imaging of RNA Processing Using Broccoli Reveals Optimal Aptamer Expression Strategies

(2015) Grigory S. Filonov et al.   CHEMISTRY & BIOLOGY

Combinatorial control ofDrosophilacircular RNA expression by intronic repeats, hnRNPs, and SR proteins

(2015) Marianne C. Kramer et al.   GENES & DEVELOPMENT

Exon Skipping Is Correlated with Exon Circularization

(2015) Steven Kelly et al.   JOURNAL OF MOLECULAR BIOLOGY

Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed

(2015) Agnieszka Rybak-Wolf et al.   MOLECULAR CELL

Neural circular RNAs are derived from synaptic genes and regulated by development and plasticity

(2015) Xintian You et al.   NATURE NEUROSCIENCE

Exon-intron circular RNAs regulate transcription in the nucleus

(2015) Zhaoyong Li et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Comparison of circular RNA prediction tools

(2015) Thomas B. Hansen et al.   NUCLEIC ACIDS RESEARCH

Circular RNAs are long-lived and display only minimal early alterations in response to a growth factor

(2015) Yehoshua Enuka et al.   NUCLEIC ACIDS RESEARCH

Transcriptome-wide investigation of circular RNAs in rice

(2015) Tingting Lu et al.   RNA

A Genetic Platform to Model Sarcomagenesis from Primary Adult Mesenchymal Stem Cells

(2015) J. Guarnerio et al.   Cancer Discovery

Exon-intron circular RNAs regulate transcription in the nucleus

(2015) Zhaoyong Li et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Exon Circularization Requires Canonical Splice Signals

(2015) Stefan Starke et al.   Cell Reports

Analysis of Intron Sequences Reveals Hallmarks of Circular RNA Biogenesis in Animals

(2015) Andranik Ivanov et al.   Cell Reports

Statistically based splicing detection reveals neural enrichment and tissue-specific induction of circular RNA during human fetal development

(2015) Linda Szabo et al.   GENOME BIOLOGY

Single-cell RNA-seq transcriptome analysis of linear and circular RNAs in mouse preimplantation embryos

(2015) Xiaoying Fan et al.   GENOME BIOLOGY

CIRI: an efficient and unbiased algorithm for de novo circular RNA identification

(2015) Yuan Gao et al.   GENOME BIOLOGY

Identification and Characterization of Circular RNAs As a New Class of Putative Biomarkers in Human Blood

(2015) Sebastian Memczak et al.   PLoS One

Correlation of circular RNA abundance with proliferation – exemplified with colorectal and ovarian cancer, idiopathic lung fibrosis and normal human tissues

(2015) Anna Bachmayr-Heyda et al.   Scientific Reports

Apoptotic Caspases Prevent the Induction of Type I Interferons by Mitochondrial DNA

(2014) Anthony Rongvaux et al.   CELL

Complementary Sequence-Mediated Exon Circularization

(2014) Xiao-Ou Zhang et al.   CELL

The Landscape of MicroRNA, Piwi-Interacting RNA, and Circular RNA in Human Saliva

(2014) J. H. Bahn et al.   CLINICAL CHEMISTRY

Short intronic repeat sequences facilitate circular RNA production

(2014) Dongming Liang et al.   GENES & DEVELOPMENT

Broccoli: Rapid Selection of an RNA Mimic of Green Fluorescent Protein by Fluorescence-Based Selection and Directed Evolution

(2014) Grigory S. Filonov et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

circRNA Biogenesis Competes with Pre-mRNA Splicing

(2014) Reut Ashwal-Fluss et al.   MOLECULAR CELL

Assessing the ceRNA Hypothesis with Quantitative Measurements of miRNA and Target Abundance

(2014) Rémy Denzler et al.   MOLECULAR CELL

Endogenous miRNA and Target Concentrations Determine Susceptibility to Potential ceRNA Competition

(2014) Andrew D. Bosson et al.   MOLECULAR CELL

Crosslinking-immunoprecipitation (iCLIP) analysis reveals global regulatory roles of hnRNP L

(2014) Oliver Rossbach et al.   RNA Biology

Lariat intronic RNAs in the cytoplasm ofXenopus tropicalisoocytes

(2014) Gaëlle J.S. Talhouarne et al.   RNA

Efficient backsplicing produces translatable circular mRNAs

(2014) Yang Wang et al.   RNA

Genome-wide Analysis of Drosophila Circular RNAs Reveals Their Structural and Sequence Properties and Age-Dependent Neural Accumulation

(2014) Jakub O. Westholm et al.   Cell Reports

Pattern Recognition and Signaling Mechanisms of RIG-I and MDA5

(2014) Stephanie Reikine et al.   Frontiers in Immunology

The “Observer Effect” in Genome-wide Surveys of Protein-RNA Interactions

(2013) Kasandra J. Riley et al.   MOLECULAR CELL

Circular Intronic Long Noncoding RNAs

(2013) Yang Zhang et al.   MOLECULAR CELL

Natural RNA circles function as efficient microRNA sponges

(2013) Thomas B. Hansen et al.   NATURE

Circular RNAs are a large class of animal RNAs with regulatory potency

(2013) Sebastian Memczak et al.   NATURE

Treatment of HCV Infection by Targeting MicroRNA

(2013) Harry L.A. Janssen et al.   NEW ENGLAND JOURNAL OF MEDICINE

Cell-Type Specific Features of Circular RNA Expression

(2013) Julia Salzman et al.   PLoS Genetics

Post-transcriptional control of gene expression by AUF1: Mechanisms, physiological targets, and regulation

(2012) Elizabeth J.F. White et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types

(2012) Julia Salzman et al.   PLoS One

Circular RNAs are abundant, conserved, and associated with ALU repeats

(2012) W. R. Jeck et al.   RNA

Cyclic GMP-AMP Synthase Is a Cytosolic DNA Sensor That Activates the Type I Interferon Pathway

(2012) L. Sun et al.   SCIENCE

Conserved Function of lincRNAs in Vertebrate Embryonic Development despite Rapid Sequence Evolution

(2011) Igor Ulitsky et al.   CELL

A ceRNA Hypothesis: The Rosetta Stone of a Hidden RNA Language?

(2011) Leonardo Salmena et al.   CELL

miRNA-dependent gene silencing involving Ago2-mediated cleavage of a circular antisense RNA

(2011) Thomas B Hansen et al.   EMBO JOURNAL

Genomewide characterization of non-polyadenylated RNAs

(2011) Li Yang et al.   GENOME BIOLOGY

RNA Targeting Therapeutics: Molecular Mechanisms of Antisense Oligonucleotides as a Therapeutic Platform

(2010) C. Frank Bennett et al.   Annual Review of Pharmacology and Toxicology

The Nuclear Envelope

(2010) M. W. Hetzer   Cold Spring Harbor Perspectives in Biology

Selection and characterization of anti-NF- B p65 RNA aptamers

(2008) S. E. Wurster et al.   RNA