Determination of intracellular protein–ligand binding affinity by competition binding in-cell NMR

Protein in-cell NMR spectroscopy at 1.2 GHz

(2021) Enrico Luchinat et al.   JOURNAL OF BIOMOLECULAR NMR

Drug Screening in Human Cells by NMR Spectroscopy Allows the Early Assessment of Drug Potency

(2020) Enrico Luchinat et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A Phase 1 Study of SLC-0111, a Novel Inhibitor of Carbonic Anhydrase IX, in Patients With Advanced Solid Tumors

(2020) Paul C. McDonald et al.   AMERICAN JOURNAL OF CLINICAL ONCOLOGY-CANCER CLINICAL TRIALS

Real-Time Quantitative In-Cell NMR: Ligand Binding and Protein Oxidation Monitored in Human Cells Using Multivariate Curve Resolution

(2020) Enrico Luchinat et al.   ANALYTICAL CHEMISTRY

Intracellular Binding/Unbinding Kinetics of Approved Drugs to Carbonic Anhydrase II Observed by in-Cell NMR

(2020) Enrico Luchinat et al.   ACS Chemical Biology

In‐cell NMR of functional riboswitch aptamers in eukaryotic cells

(2020) P. Broft et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Carbonic Anhydrase Inhibitors Targeting Metabolism and Tumor Microenvironment

(2020) Andrea Angeli et al.   Metabolites

Nanoscale Ion Emitters in Native Mass Spectrometry for Measuring Ligand–Protein Binding Affinities

(2019) Giang T. H. Nguyen et al.   ACS Central Science

Cadmium effects on superoxide dismutase 1 in human cells revealed by NMR

(2019) Panagis Polykretis et al.   Redox Biology

Fast NMR methods for measuring in direct and/or competition mode the dissociation binding constants of chemical fragments interacting with a receptor

(2019) Alexey Rak et al.   ChemMedChem

Assessment of a Large Enzyme-Drug Complex by Proton-Detected Solid-State NMR Spectroscopy without Deuteration

(2019) Suresh K. Vasa et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

High-Resolution Protein 3D Structure Determination in Living Eukaryotic Cells

(2019) Takashi Tanaka et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Improving the sensitivity of FT-NMR spectroscopy by apodization weighted sampling

(2019) Bernd Simon et al.   JOURNAL OF BIOMOLECULAR NMR

Cells, drugs and NMR

(2019) Gregg Siegal et al.   JOURNAL OF MAGNETIC RESONANCE

Advances in the structural annotation of human carbonic anhydrases and impact on future drug discovery

(2019) Alessio Nocentini et al.   Expert Opinion on Drug Discovery

Monitoring DNA–Ligand Interactions in Living Human Cells Using NMR Spectroscopy

(2019) Michaela Krafcikova et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

In-Cell NMR in Human Cells: Direct Protein Expression Allows Structural Studies of Protein Folding and Maturation

(2018) Enrico Luchinat et al.   ACCOUNTS OF CHEMICAL RESEARCH

Potent Inhibitors of Mycobacterium tuberculosis Growth Identified by Using in-Cell NMR-based Screening

(2018) Christopher M. DeMott et al.   ACS Chemical Biology

Evaluation of the Stability of DNA i-Motifs in the Nuclei of Living Mammalian Cells

(2018) Simon Dzatko et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Real-Time In-Cell Nuclear Magnetic Resonance: Ribosome-Targeted Antibiotics Modulate Quinary Protein Interactions

(2018) Leonard Breindel et al.   BIOCHEMISTRY

Introduction of Intrinsic Kinetics of Protein–Ligand Interactions and Their Implications for Drug Design

(2018) Vaida Linkuvienė et al.   JOURNAL OF MEDICINAL CHEMISTRY

The cysteine-reactive small molecule ebselen facilitates effective SOD1 maturation

(2018) Michael J. Capper et al.   Nature Communications

Probing the interaction of a quercetin bioconjugate with Bcl-2 in living human cancer cells with in-cell NMR spectroscopy

(2018) Alexandra Primikyri et al.   FEBS LETTERS

Target Engagement and Binding Mode of an Antituberculosis Drug to Its Bacterial Target Deciphered in Whole Living Cells by NMR

(2018) Guillaume Bouvier et al.   BIOCHEMISTRY

High-Resolution Solid-State NMR Characterization of Ligand Binding to a Protein Immobilized in a Silica Matrix

(2017) Linda Cerofolini et al.   JOURNAL OF PHYSICAL CHEMISTRY B

A molecular chaperone activity of CCS restores the maturation of SOD1 fALS mutants

(2017) Enrico Luchinat et al.   Scientific Reports

Structure and function of carbonic anhydrases

(2016) C. T. Supuran   BIOCHEMICAL JOURNAL

Structural disorder of monomeric α-synuclein persists in mammalian cells

(2016) Francois-Xavier Theillet et al.   NATURE

Characterization of proteins by in-cell NMR spectroscopy in cultured mammalian cells

(2016) Letizia Barbieri et al.   Nature Protocols

In-cell thermodynamics and a new role for protein surfaces

(2016) Austin E. Smith et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Probing Protein Quinary Interactions by In-Cell Nuclear Magnetic Resonance Spectroscopy

(2015) Subhabrata Majumder et al.   BIOCHEMISTRY

MCR-ALS GUI 2.0: New features and applications

(2015) Joaquim Jaumot et al.   CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS

The drug–target residence time model: a 10-year retrospective

(2015) Robert A. Copeland   NATURE REVIEWS DRUG DISCOVERY

Intrinsic thermodynamics of sulfonamide inhibitor binding to human carbonic anhydrases I and II

(2014) Vaida Morkūnaitė et al.   JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY

High-Resolution Heteronuclear Multidimensional NMR of Proteins in Living Insect Cells Using a Baculovirus Protein Expression System

(2013) Jumpei Hamatsu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Gel-Encapsulated Bioreactor System for NMR Studies of Protein-Protein Interactions in Living Mammalian Cells

(2012) Satoshi Kubo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Cell death assays for drug discovery

(2011) Oliver Kepp et al.   NATURE REVIEWS DRUG DISCOVERY

Principles of early drug discovery

(2010) JP Hughes et al.   BRITISH JOURNAL OF PHARMACOLOGY

Measurement of Nanomolar Dissociation Constants by Titration Calorimetry and Thermal Shift Assay – Radicicol Binding to Hsp90 and Ethoxzolamide Binding to CAII

(2009) Asta Zubrienė et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Observation of NMR Signals from Proteins Introduced into Living Mammalian Cells by Reversible Membrane Permeabilization Using a Pore-Forming Toxin, Streptolysin O

(2009) Shinji Ogino et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Protein structure determination in living cells by in-cell NMR spectroscopy

(2009) Daisuke Sakakibara et al.   NATURE

High-resolution multi-dimensional NMR spectroscopy of proteins in human cells

(2009) Kohsuke Inomata et al.   NATURE

Carbonic anhydrases: novel therapeutic applications for inhibitors and activators

(2008) Claudiu T. Supuran   NATURE REVIEWS DRUG DISCOVERY