Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli

The impact of genetic diversity on gene essentiality within the Escherichia coli species

(2021) François Rousset et al.   Nature Microbiology

On-target activity predictions enable improved CRISPR–dCas9 screens in bacteria

(2020) Alicia Calvo-Villamañán et al.   NUCLEIC ACIDS RESEARCH

Repurposing the Streptococcus mutans CRISPR-Cas9 System to Understand Essential Gene Function

(2020) Robert C. Shields et al.   PLoS Pathogens

High-throughput mapping of the phage resistance landscape in E. coli

(2020) Vivek K. Mutalik et al.   PLOS BIOLOGY

Mismatch-CRISPRi Reveals the Co-varying Expression-Fitness Relationships of Essential Genes in Escherichia coli and Bacillus subtilis

(2020) John S. Hawkins et al.   Cell Systems

A simplified strategy for titrating gene expression reveals new relationships between genotype, environment, and bacterial growth

(2020) Andrew D Mathis et al.   NUCLEIC ACIDS RESEARCH

Multi-omics Analysis of CRISPRi-Knockdowns Identifies Mechanisms that Buffer Decreases of Enzymes in E. coli Metabolism

(2020) Stefano Donati et al.   Cell Systems

Bacterial CRISPR screens for gene function

(2020) Horia Todor et al.   CURRENT OPINION IN MICROBIOLOGY

(p)ppGpp-mediated stress response induced by defects in outer membrane biogenesis and ATP production promotes survival in Escherichia coli

(2019) Mohammad Roghanian et al.   Scientific Reports

Simultaneous repression of multiple bacterial genes using nonrepetitive extra-long sgRNA arrays

(2019) Alexander C. Reis et al.   NATURE BIOTECHNOLOGY

Evolutionary Convergence of Pathway-Specific Enzyme Expression Stoichiometry

(2018) Jean-Benoît Lalanne et al.   CELL

How to Build a Bacterial Cell: MreB as the Foreman of E. coli Construction

(2018) Handuo Shi et al.   CELL

Genomewide phenotypic analysis of growth, cell morphogenesis, and cell cycle events in Escherichia coli

(2018) Manuel Campos et al.   Molecular Systems Biology

Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes

(2018) Antoine Vigouroux et al.   Molecular Systems Biology

Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance

(2018) Tianmin Wang et al.   Nature Communications

A CRISPRi screen in E. coli reveals sequence-specific toxicity of dCas9

(2018) Lun Cui et al.   Nature Communications

Mutant phenotypes for thousands of bacterial genes of unknown function

(2018) Morgan N. Price et al.   NATURE

The outer membrane is an essential load-bearing element in Gram-negative bacteria

(2018) Enrique R. Rojas et al.   NATURE

Genome-wide CRISPR-dCas9 screens in E. coli identify essential genes and phage host factors

(2018) François Rousset et al.   PLoS Genetics

Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi

(2018) Jason M. Peters et al.   Nature Microbiology

High‐throughput CRISPRi phenotyping identifies new essential genes in Streptococcus pneumoniae

(2017) Xue Liu et al.   Molecular Systems Biology

The mecillinam resistome reveals a role for peptidoglycan endopeptidases in stimulating cell wall synthesis in Escherichia coli

(2017) Ghee Chuan Lai et al.   PLoS Genetics

Construction and Analysis of Two Genome-Scale Deletion Libraries for Bacillus subtilis

(2017) Byoung-Mo Koo et al.   Cell Systems

Repression of YdaS Toxin Is Mediated by Transcriptional Repressor RacR in the Cryptic rac Prophage of Escherichia coli K-12

(2017) Revathy Krishnamurthi et al.   mSphere

A Comprehensive, CRISPR-based Functional Analysis of Essential Genes in Bacteria

(2016) Jason M. Peters et al.   CELL

Massively Parallel Interrogation of the Effects of Gene Expression Levels on Fitness

(2016) Leeat Keren et al.   CELL

Interrupting Biosynthesis of O Antigen or the Lipopolysaccharide Core Produces Morphological Defects in Escherichia coli by Sequestering Undecaprenyl Phosphate

(2016) Matthew A. Jorgenson et al.   JOURNAL OF BACTERIOLOGY

Deep Learning Automates the Quantitative Analysis of Individual Cells in Live-Cell Imaging Experiments

(2016) David A. Van Valen et al.   PLoS Computational Biology

Bacterial CRISPR: accomplishments and prospects

(2015) Jason M Peters et al.   CURRENT OPINION IN MICROBIOLOGY

Riboregulation of the bacterial actin-homolog MreB by DsrA small noncoding RNA

(2015) Bastien Cayrol et al.   Integrative Biology

Dead-end intermediates in the enterobacterial common antigen pathway induce morphological defects inEscherichia coliby competing for undecaprenyl phosphate

(2015) Matthew A. Jorgenson et al.   MOLECULAR MICROBIOLOGY

Colony-live —a high-throughput method for measuring microbial colony growth kinetics— reveals diverse growth effects of gene knockouts in Escherichia coli

(2014) Rikiya Takeuchi et al.   BMC MICROBIOLOGY

Systematic Perturbation of Cytoskeletal Function Reveals a Linear Scaling Relationship between Cell Geometry and Fitness

(2014) Russell D. Monds et al.   Cell Reports

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Flavoproteins Are Potential Targets for the Antibiotic Roseoflavin in Escherichia coli

(2013) S. Langer et al.   JOURNAL OF BACTERIOLOGY

CRISPR interference (CRISPRi) for sequence-specific control of gene expression

(2013) Matthew H Larson et al.   Nature Protocols

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

Bacterial stress responses as determinants of antimicrobial resistance

(2012) K. Poole   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Lag Phase Is a Distinct Growth Phase That Prepares Bacteria for Exponential Growth and Involves Transient Metal Accumulation

(2011) M. D. Rolfe et al.   JOURNAL OF BACTERIOLOGY

High-throughput, subpixel precision analysis of bacterial morphogenesis and intracellular spatio-temporal dynamics

(2011) Oleksii Sliusarenko et al.   MOLECULAR MICROBIOLOGY

The bacterial actin MreB rotates, and rotation depends on cell-wall assembly

(2011) S. van Teeffelen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Phenotypic Landscape of a Bacterial Cell

(2010) Robert J. Nichols et al.   CELL

The MIQE Guidelines: Minimum Information for Publication of Quantitative Real-Time PCR Experiments

(2009) S. A. Bustin et al.   CLINICAL CHEMISTRY

BolA Inhibits Cell Elongation and Regulates MreB Expression Levels

(2008) Patrick Freire et al.   JOURNAL OF MOLECULAR BIOLOGY

High-throughput, quantitative analyses of genetic interactions in E. coli

(2008) Athanasios Typas et al.   NATURE METHODS

The Stringent Response and Cell Cycle Arrest in Escherichia coli

(2008) Daniel J. Ferullo et al.   PLoS Genetics