PAM-less conditional DNA substrates leverage trans-cleavage of CRISPR-Cas12a for versatile live-cell biosensing

Structure of human telomerase holoenzyme with bound telomeric DNA

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Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level

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Single Virus Tracking with Quantum Dots Packaged into Enveloped Viruses Using CRISPR

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Conditional control of RNA-guided nucleic acid cleavage and gene editing

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CRISPR–Cas12-based detection of SARS-CoV-2

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DNA Functional Materials Assembled from Branched DNA: Design, Synthesis, and Applications

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Very fast CRISPR on demand

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Development of CRISPR-Cas13a-based antimicrobials capable of sequence-specific killing of target bacteria

(2020) Kotaro Kiga et al.   Nature Communications

CRISPR-Cas9 Circular Permutants as Programmable Scaffolds for Genome Modification

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Mechanistic Insights into the cis- and trans-Acting DNase Activities of Cas12a

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Progress and Challenges for Live-cell Imaging of Genomic Loci Using CRISPR-based Platforms

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DNA-based communication in populations of synthetic protocells

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The next generation of CRISPR–Cas technologies and applications

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Switching the activity of Cas12a using guide RNA strand displacement circuits

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Conditional Guide RNAs: Programmable Conditional Regulation of CRISPR/Cas Function in Bacterial and Mammalian Cells via Dynamic RNA Nanotechnology

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Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs

(2019) Xianjun Chen et al.   NATURE BIOTECHNOLOGY

A split CRISPR–Cpf1 platform for inducible genome editing and gene activation

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CRISPR-mediated live imaging of genome editing and transcription

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Exploring the Trans-Cleavage Activity of CRISPR-Cas12a (cpf1) for the Development of a Universal Electrochemical Biosensor

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A CRISPR-Cas12a-derived biosensing platform for the highly sensitive detection of diverse small molecules

(2019) Mindong Liang et al.   Nature Communications

Functional DNA Regulated CRISPR-Cas12a Sensors for Point-of-Care Diagnostics of Non-Nucleic-Acid Targets

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Dynamic Imaging of RNA in Living Cells by CRISPR-Cas13 Systems

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Probing and engineering liquid-phase organelles

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FRET-based nucleic acid probes: Basic designs and applications in bioimaging

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Genomes in Focus: Development and Applications of CRISPR-Cas9 Imaging Technologies

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CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

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Protein Phase Separation: A New Phase in Cell Biology

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Direct observation of DNA target searching and cleavage by CRISPR-Cas12a

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CRISPR-Cas12a-assisted nucleic acid detection

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Direct Visualization of Single-Nucleotide Variation in mtDNA Using a CRISPR/Cas9-Mediated Proximity Ligation Assay

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Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity

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RNA targeting with CRISPR–Cas13

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Structure-guided chemical modification of guide RNA enables potent non-viral in vivo genome editing

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ATP-dependent modulation of MgtE in Mg2+ homeostasis

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Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation

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DNA nanotechnology

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Programmable RNA Tracking in Live Cells with CRISPR/Cas9

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Crystal Structure of Cpf1 in Complex with Guide RNA and Target DNA

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The crystal structure of Cpf1 in complex with CRISPR RNA

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C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector

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DNA-based ATP sensing

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Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

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Optical Control of CRISPR/Cas9 Gene Editing

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Discovery and Functional Characterization of Diverse Class 2 CRISPR-Cas Systems

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DNA nanotechnology from the test tube to the cell

(2015) Yuan-Jyue Chen et al.   Nature Nanotechnology

An updated evolutionary classification of CRISPR–Cas systems

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A Robust Probe for Lighting Up Intracellular Telomerase via Primer Extension To Open a Nicked Molecular Beacon

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Dynamic Imaging of Genomic Loci in Living Human Cells by an Optimized CRISPR/Cas System

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MicroRNA: Function, Detection, and Bioanalysis

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Oligomycin frames a common drug-binding site in the ATP synthase

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Dynamic DNA nanotechnology using strand-displacement reactions

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Going deeper than microscopy: the optical imaging frontier in biology

(2010) Vasilis Ntziachristos   NATURE METHODS

The fluorescent protein palette: tools for cellular imaging

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Small CRISPR RNAs Guide Antiviral Defense in Prokaryotes

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