Article
Agriculture, Dairy & Animal Science
Bence Somoskoi, Lilla Bordas, Fusa Uno, Dora Kispal, Linda Muller, Dora Torok, Sandor Cseh
Summary: The present study aimed to compare the effects of different cryopreservation methods (closed vs open vitrification) on the survival and developmental rate of canine isolated preantral follicles (PAFs). The results showed that the OPS method provided superior survival rate, in vitro growth, and hormonal production compared to the CT method. This is the first study to compare cryopreservation protocols for canine isolated preantral follicles.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Reproductive Biology
I. P. Silva, D. C. C. Brito, T. E. S. Silva, R. F. Silva, M. I. F. Guedes, J. Y. G. Silva, A. P. R. Rodrigues, R. R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of ZEN and ENL, alone or in combination, on the survival and morphology of in vitro cultured ovarian preantral follicles. The results showed that while ZEN impaired the morphology of follicles, ENL improved the cultured secondary follicles. However, the combination of ENL with ZEN impaired the quality of primary and secondary follicles.
Article
Agriculture, Dairy & Animal Science
P. S. P. Gupta, P. Johnson, K. Kaushik, K. Krishna, S. Nandi, S. Mondal, J. Nikhil Kumar Tej, B. Somoskoi, S. Cseh
Summary: The study showed that supplementation of retinol in the vitrification solution could improve the viability of buffalo preantral follicles and enhance the expression of key genes. The addition of retinol led to significantly lower expression of apoptosis-related genes in the follicles.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Multidisciplinary Sciences
Conghui Tian, Lingxiao Shen, Chenjia Gong, Yunxia Cao, Qinghua Shi, Gang Zhao
Summary: This study achieved low-concentration cryopreservation of preantral follicles by using microencapsulation and nanowarming. The research found that this method improved the viability of preantral follicles and maintained their ability to form cavities, compared to conventional water bath warming. Furthermore, it showed no significant impact on in vitro fertilization and embryo transfer.
NATURE COMMUNICATIONS
(2022)
Article
Reproductive Biology
Lilla Bordas, Bence Somoskoi, Dora Torok, Boglarka Nora Vincze, Sandor Cseh
Summary: There is growing interest in the application of ovarian preantral follicles in reproductive science. Cryopreservation and in vitro culture of these follicles can support fertility preservation in animals with high genetic value, endangered or zoo animals, and women before anticancer therapy. However, there is currently no standardized freezing or vitrification protocol available. This study aimed to examine the viability of preantral follicles cryopreserved using cryotube freezing or OPS vitrification protocols.
REPRODUCTIVE BIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Daniela Bebbere, Amir Arav, Stefano Mario Nieddu, Giovanni Pietro Burrai, Sara Succu, Pasquale Patrizio, Sergio Ledda
Summary: Freeze-drying is a method for preserving cells and tissues, offering cost savings and improved stability; sheep ovarian tissue survived the process, maintaining its structure and responding to specific genes.
Article
Reproductive Biology
Hongyu Liu, Ayman Mesalam, Myeong-Don Joo, Shimin Zhang, Lianguang Xu, Jun Wang, Kyeong-Lim Lee, Seok-Hwan Song, Yu-Guo Yuan, Wenfa Lu, Il-Keun Kong
Summary: The study demonstrated the importance of fibronectin in bovine preantral follicle development and revealed that fibronectin could neutralize the deleterious consequences of kisspeptin, suggesting a potential role in the regulation of the PI3K/Sirt1 signaling pathway in bovine preantral follicle development.
Article
Reproductive Biology
Juliana Z. Almeida, L. A. Vieira, C. Maside, A. C. A. Ferreira, N. A. R. Sa, H. H. Correia, V. R. Araujo, R. S. Raposo, J. Smitz, C. C. Campello, J. R. Figueiredo, R. B. Oria
Summary: 5FU does not affect follicular morphology but detrimentally affects follicular growth, estradiol production, and oocyte maturation in isolated ovarian follicles. Concentrations of 5FU greater than 3 mM reduce estradiol levels.
Review
Reproductive Biology
Regina Lucia dos Santos Silva, Ricassio de Sousa Barberino, Maria Helena Tavares de Matose
Summary: The in vitro culture of ovarian preantral follicles allows studying follicular and oocyte growth, using immature oocytes as a potential source for fertilization and screening of ovarian toxic substances. However, oxidative stress caused by reactive oxygen species (ROS) accumulation poses a challenge to the success of the culture system. The addition of antioxidant agents to the culture medium can minimize the damage caused by ROS, supporting follicular survival and development, and producing mature oocytes suitable for fertilization.
Article
Biology
Kalpana Kaushik, P. S. P. Gupta, P. Johnson, Kavya Krishna, S. Nandi, S. Mondal, J. Nikhil Kumar Tej, Somoskoi Bence, Sandor Cseh
Summary: Supplementation of retinol in the vitrification solution can enhance the viability of vitrified sheep preantral follicles and affect the expression of apoptosis and development-related genes.
Article
Oncology
Juliana Z. Almeida, Laritza F. Lima, Luis A. Vieira, Carolina Maside, Anna C. A. Ferreira, Valdevane R. Araujo, Ana B. G. Duarte, Ramon S. Raposo, Sonia N. Bao, Claudio C. Campello, Luiz F. S. Oliveira, Thayse P. Costa, Jose Garcia Abreu, Jose R. Figueiredo, Reinaldo B. Oria
Summary: The study found that 5-FU can adversely affect the ovarian follicle reserve by reducing the survival of preantral follicles, leading to a significantly lower percentage of normal follicles of all types and impacting the survival of cultured normal primordial follicles.
CANCER CHEMOTHERAPY AND PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Tayebeh Dahmardeh, Mohammad Hossein Ghanian, Bita Ebrahimi
Summary: This study evaluated the potential of a hydrogel composed of thiolated hyaluronic acid (HA-SH) for ovine preantral follicle development compared to alginate hydrogel (ALG). The results showed that HA-SH hydrogel provided better support for follicle growth and had advantages in gene expression levels.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Reproductive Biology
S. Kehoe, K. Jewgenow, P. R. Johnston, B. C. Braun
Summary: The study reveals that early ovarian follicles of domestic cats express a variety of sex hormone and gonadotropin receptors, indicating their potential role in in vitro growth processes.
BIOLOGY OF REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
D. A. Cardone, A. R. R. Caceres, M. A. Sanhueza, F. A. Bruna, M. R. Laconi
Summary: Heat stress has a significant impact on the number and size of bovine primordial follicles, as well as the number of granulosa cells and the rate of follicle degeneration. These findings provide valuable insights into the effects of heat stress on the reproductive performance of cattle.
Article
Andrology
Sanaz Alaee, Raheleh Asadollahpour, Abasalt Hosseinzadeh Colagar, Tahereh Talaei-Khozani
Summary: The study utilized a decellularized ovary scaffold for the encapsulation and development of preantral follicles. The results indicated that follicles cultured in 3D conditions had significantly higher survival rate, antrum formation rate, E2 and P4 concentrations, and oocyte maturation rate compared to 2D conditions, while gene expression levels showed no significant differences across different culture conditions.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2021)
Article
Reproductive Biology
Haolin Li, Xiaomeng Pei, Hao Yu, Wei Wang, Dagan Mao
Summary: This study investigated the effects of APN/AdipoRon on autophagy and apoptosis in goat luteal cells, and found that autophagy and apoptosis play important roles in the structural regression of goat corpus luteum.
Article
Reproductive Biology
Yan-Qiu Wang, He-Xuan Qu, Yan-Wei Dong, Jia-Jia Qi, Hua-Kai Wei, Hao Sun, Hao Jiang, Jia-Bao Zhang, Bo-Xing Sun, Shuang Liang
Summary: This research reveals the crucial role of Ferroptosis Suppressor Protein 1 (FSP1) in regulating the development and quality of porcine early embryos. Inhibition of FSP1 impairs blastocyst formation, induces glutathione-independent ferroptosis, and leads to oxidative stress due to mitochondrial dysfunction.
Article
Reproductive Biology
Xiong Lai, Ruizhuo Liu, Mengyu Li, Yaochun Fan, Hongxia Li, Guotao Han, Ruijie Guo, Hairui Ma, Huimin Su, Wanjin Xing
Summary: This study identifies WDR54 as a protein involved in sperm-oocyte fertilization and shows that it interacts with IZUMO1 and JUNO. The formation of the JWIJ complex on the oocyte surface suggests its importance in sperm-oocyte adhesion and fusion. This discovery provides new insights into the mechanisms of mammalian sperm-oocyte adhesion and fusion.
Article
Reproductive Biology
S. Nandi, B. Sampath Kumar, P. S. P. Gupta, S. Mondal, V. Girish Kumar
Summary: This study examined the effects of plant bioactive compounds on the survival and growth parameters of ovine preantral follicles granulosa cells (GCs) and the expression of key developmental genes. The results showed that KAE, MYR and QUE had dose-dependent responses on the morphological and functional parameters of the cells, with KAE being the most potent in augmenting ovarian functions.
Article
Reproductive Biology
Francisco E. Martin-Cano, Gemma Gaitskell-Phillips, Laura Becerro-Rey, Eva da Silva, Javier Masot, Eloy Redondo, Antonio Silva-Rodriguez, Cristina Ortega-Ferrusola, Maria Cruz Gil, Fernando J. Pena
Summary: The presence of a more efficient glycolysis mechanism depending on pyruvate in stallion spermatozoa can counteract the detrimental effects of higher glucose concentrations. Incubating spermatozoa with 10 mM pyruvate in a modified Tyrode's media with 67 mM glucose showed increased motility and maintained viability. This improvement is attributed to the conversion of lactate to pyruvate and increased NAD(+) enhancing glycolysis efficiency.
Article
Reproductive Biology
Thanida Sananmuang, Denis Puthier, Catherine Nguyen, Kaj Chokeshaiusaha
Summary: Ongoing progress in mRNA-Sequencing technologies has contributed to the refinement of assisted reproductive technologies. This study utilized mRNA-Seq datasets of various mammalian species to analyze differential transcript usage (DTU) and identified ten cross-species DTU genes. Gene Ontology (GO) term enrichment analysis revealed the involvement of these genes in RNA and cell-cycle control mechanisms, suggesting their importance during oocyte maturation. Further exploration of these genes' transcript isoforms may lead to the discovery of novel markers for reproductive technologies.
Article
Reproductive Biology
Chenglei Song, Zhe Zhang, Yilin Wei, Yaqing Dou, Kunlong Qi, Xiuling Li, Feng Yang, Xinjian Li, Kejun Wang, Ruimin Qiao, Xuelei Han
Summary: In this study, the changes in protein levels in boar sperm with different liquid preservation abilities were analyzed. Key proteins related to sperm liquid preservation ability were identified. The findings provide new insights into the molecular mechanisms of boar sperm liquid preservation ability.
Article
Reproductive Biology
Genevieve M. VanWye, Emily G. Smith, Christine Spinka, Michael F. Smith, Matthew C. Lucy, Jordan M. Thomas
Summary: A series of experiments evaluated treatment schedules for controlling the estrous cycle in beef heifers. The results showed that the duration of progestin treatment and the timing of prostaglandin administration had an impact on estrus expression and pregnancy rates.
Article
Reproductive Biology
Hongwei Duan, Shuai Yang, Longfei Xiao, Shanshan Yang, Zhenxing Yan, Fang Wang, Xiaofei Ma, Lihong Zhang, Yong Zhang, Junjie Hu, Xingxu Zhao
Summary: This study first elucidated the mechanism by which melatonin regulates progesterone secretion in the corpus luteum of sheep, namely by promoting progesterone secretion through the regulation of autophagy. This has important implications for further exploring the role of melatonin in regulating sheep ovarian function.
Article
Reproductive Biology
Zhijie Zheng, Yingfang Guo, Yonghui Zheng, Haichong Wu
Summary: This study demonstrates that miR-92b plays a protective role in LTA-induced endometritis by targeting FZD10 and reducing the production of pro-inflammatory cytokines, thus alleviating uterine injury.
Article
Reproductive Biology
Areeg Almubarak, Sanghoon Lee, Il-Jeoung Yu, Yubyeol Jeon
Summary: This study found that Nobiletin (NOB) can promote the cryosurvival and embryo development of porcine sperm after in vitro fertilization (IVF). The addition of NOB improves sperm motility, acrosome integrity, viability, and mitochondrial membrane potential, reduces lipid peroxidation, and increases cleavage and blastocyst formation rates. Furthermore, NOB treatment upregulates the expression of genes related to pluripotency and cell differentiation in the resulting embryos.
Article
Reproductive Biology
Yuan Li, Lishu Li, Wenjie Xiong, Xing Duan, Huaming Xi
Summary: This study investigated the cytotoxicity and toxicological mechanisms of fluorochloridone (FLC) on cultured goat Sertoli cells. The results showed that FLC exposure induced oxidative stress, promoted apoptosis and autophagy, inhibited lysosomal biogenesis, and blocked autophagic flux in goat Sertoli cells. FLC also caused mitochondrial dysfunction and disturbed mitophagy, leading to cell apoptosis. The antioxidant N-acetyl-L-cysteine (NAC) reduced FLC-induced ROS accumulation and reversed the disorder of autophagy levels.
Article
Reproductive Biology
Gabriela Ramos Leal, Thais de Almeida Oliveira, Mariana Pedrosa de Paula Guimaraes, Lucas Francisco Leodido Correia, Erlandia Marcia Vasconcelos, Joanna Maria Goncalves Souza-Fabjan
Summary: This study investigated the time course of lipid accumulation during IVM and the effects of lipid modulators on cat oocytes. The results showed that adding lipid modulators can reduce lipid content and improve viability after cryopreservation.
Article
Reproductive Biology
Isabella Rio Feltrin, Amanda Guimaraes da Silva, Cecilia Constantino Rocha, Priscila Assis Ferraz, Paola Maria da Silva Rosa, Thiago Martins, Juliano Coelho da Silveira, Milena Lopes Oliveira, Mario Binelli, Guilherme Pugliesi, Claudia Maria Bertan Membrive
Summary: The study evaluated the effects of E2 treatment on Nelore heifers on day 15 of the estrous cycle, finding that E2 promoted an increase in PGFM concentrations and accelerated functional and structural luteolysis by upregulating the expression of PGR and OXTR. This suggests that within 3 hours after E2 stimulus, the expression of these receptors is associated with triggering luteolysis in cattle.
Article
Reproductive Biology
Aline Matos Arrais, Angelo Jose Burla Dias, Claudio Luiz Melo de Souza, Alinne Gloria Curcio, Marco Roberto Bourg de Mello
Summary: This study evaluated the effect of phospholipase C (PLC) on the capacitation of cryopreserved ovine semen. The results showed that the inhibitor U73122 can prevent premature capacitation and acrosome reaction induced by the freezing procedure, without affecting the kinetics and integrity of the sperm membranes.