Article
Multidisciplinary Sciences
Takeru Ishizaki, Yasuto Takeuchi, Kojiro Ishibashi, Noriko Gotoh, Eishu Hirata, Kosuke Kuroda
Summary: Cryopreservation of tissues is a difficult challenge. Vitrification has recently emerged as a suitable method for tissue cryopreservation, while slow-freezing also shows potential. In this study, an aqueous solution containing a novel cryoprotectant, combined with DMSO, improved the cryopreservation of cell spheroids compared to a commercial cryoprotectant. The optimized solution composition was 10 wt% zwitterion, 15 wt% DMSO, and 75 wt% water.
SCIENTIFIC REPORTS
(2023)
Article
Reproductive Biology
P. S. P. Gupta, Kalpana Kaushik, P. Johnson, Kavya Krishna, S. Nandi, S. Mondal, J. Nikhil Kumar Tej, B. Somoskoi, S. Cseh
Summary: The viability of preantral follicles was negatively affected by the low concentration of cryoprotectants in vitrification treatment B, while treatment A and C showed no significant difference compared to the control group. The expression of the apoptotic gene BAK was higher in treatment B, while the development competence genes GDF-9 and BMP-15 showed higher expression in treatment A and C, respectively. The NOBOX gene expression was significantly increased in preantral follicles with treatment B.
Article
Agriculture, Dairy & Animal Science
Bence Somoskoi, Lilla Bordas, Fusa Uno, Dora Kispal, Linda Muller, Dora Torok, Sandor Cseh
Summary: The present study aimed to compare the effects of different cryopreservation methods (closed vs open vitrification) on the survival and developmental rate of canine isolated preantral follicles (PAFs). The results showed that the OPS method provided superior survival rate, in vitro growth, and hormonal production compared to the CT method. This is the first study to compare cryopreservation protocols for canine isolated preantral follicles.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Multidisciplinary Sciences
Takashi Yoshino, Takahiro Suzuki, Go Nagamatsu, Haruka Yabukami, Mika Ikegaya, Mami Kishima, Haruka Kita, Takuya Imamura, Kinichi Nakashima, Ryuichi Nishinakamura, Makoto Tachibana, Miki Inoue, Yuichi Shima, Ken-ichirou Morohashi, Katsuhiko Hayashi
Summary: Novel methods have been developed to generate functional mouse oocytes from pluripotent stem cell-derived primordial germ cell-like cells (PGCLCs) and fetal ovarian somatic cell-like cells (FOSLCs). When FOSLCs are aggregated with PGCLCs, they enter meiosis and produce oocytes capable of fertilization and development into live offspring, providing a method for in vitro oocyte production and follicle generation for a better understanding of mammalian reproduction.
Review
Obstetrics & Gynecology
Marisa Kometas, Gregory M. Christman, Joseph Kramer, Alice Rhoton-Vlasak
Summary: The effectiveness of vitrification compared to slow freezing for preserving ovarian tissue remains controversial, with limited clinical data and inconsistent findings from biochemical studies. While early clinical outcomes suggest favorability towards vitrification, further evaluation is needed to determine its long-term efficacy. Despite potential lack of difference in outcomes between the two methods, the cost-effectiveness and efficiency of vitrification make it a more accessible option.
REPRODUCTIVE SCIENCES
(2021)
Article
Andrology
Dai Zhou, Xing-Ming Wang, Rui-Xue Li, Yi-Ze Wang, Yuan-Chi Chao, Zhi-Zhong Liu, Zeng-Hui Huang, Hong-Chuan Nie, Wen-Bing Zhu, Yue-Qiu Tan, Li-Qing Fan
Summary: A modified vitrification method for native spermatozoa was developed in this study, showing better protective effects on several sperm parameters compared with slow freezing. Vitrification achieved higher recovery rates, motility, morphology, and curve line velocity, as well as decreased DNA fragmentation and better acrosome protection in spermatozoa.
ASIAN JOURNAL OF ANDROLOGY
(2021)
Review
Cell Biology
Amir Arav
Summary: This review discusses the current status and key factors of cryopreservation of large tissues and organs, introducing the principles of directional freezing and vitrification, and summarizing the ways in which cryopreservation affects large tissues and organs.
Article
Reproductive Biology
M. Umair, M. Beitsma, M. de Ruijter-Villani, C. Deelen, C. Herrera, T. A. E. Stout, A. Claes
Summary: The cryotolerance of equine blastocysts larger than 300 mu m can be improved by aspirating blastocoele fluid prior to vitrification; however, it is not known whether blastocoele aspiration also enables successful slow-freezing. The aim of this study was therefore to determine whether slow-freezing of expanded equine embryos following blastocoele collapse was more or less damaging than vitrification.
Article
Veterinary Sciences
Martina Colombo, Maria Giorgia Morselli, Jennifer Zahmel, Gaia Cecilia Luvoni
Summary: The aim of this study was to evaluate the morphofunctional integrity of cat epididymal spermatozoa ultra-rapid frozen in different extenders and to test whether spermatozoa preserved by the best combination were able to fertilize oocytes and produce embryos in vitro by intracytoplasmic sperm injection (ICSI). The results showed that cat epididymal spermatozoa better maintained their morphofunctional features after ultra-rapid freezing with a specific extender and could successfully produce embryos in vitro after ICSI.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Conghui Tian, Lingxiao Shen, Chenjia Gong, Yunxia Cao, Qinghua Shi, Gang Zhao
Summary: This study achieved low-concentration cryopreservation of preantral follicles by using microencapsulation and nanowarming. The research found that this method improved the viability of preantral follicles and maintained their ability to form cavities, compared to conventional water bath warming. Furthermore, it showed no significant impact on in vitro fertilization and embryo transfer.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
A. Kocabay, A. C. Taskin
Summary: This study investigates the development and quality of single-cell mouse embryos in a boric acid-added culture medium after cryopreservation using the solid-surface vitrification method. The results suggest that the addition of boric acid to the culture media increased the development rate of the embryos that were vitrified using the SSV method.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Obstetrics & Gynecology
Barbara Rodrigues Nascimento, Danielle Storino de Freitas, Julia Meireles Nogueira, Clara Carvalho e Souza, Rayan Silva de Paula, Jousie Michel Pereira, Ana Paula Madureira, Luciola Silva Barcelos, Erika Cristina Jorge, Paulo Henrique Almeida Campos-Junior
Summary: Ovarian cryopreservation is a viable option for fertility preservation and subcutaneous transplantation is a promising site. This study aimed to evaluate the effect of subcutaneous autotransplantation and vitrification on follicular growth and atresia, as well as their relationship with vascular perfusion and gene expression. The results showed that the first day after subcutaneous transplantation is critical for follicular survival, with intense follicular atresia independent of Bax upregulation.
REPRODUCTIVE SCIENCES
(2023)
Article
Cell Biology
Longping Liu, Ming Hao, Jianyun Zhang, Ziqi Chen, Jiaqi Zhou, Chao Wang, Hua Zhang, Jianbin Wang
Summary: Using a high-throughput molecular pathology strategy, we have characterized the transcriptional atlas of mouse follicular granulosa cells under physiological conditions and identified the molecular mechanisms behind follicle selection and atresia. Our findings suggest that hypoxia-inducible factor 1 (HIF1) is a major downstream effector of follicle-stimulating hormone (FSH), essential for follicle growth. Energy shortage leads to prevalent activation of AMP-activated protein kinase (AMPK) and drives follicular atresia. FSHR-mTOR-HIF1 signaling helps follicles escape from atresia fate under persistent energy stress.
Article
Obstetrics & Gynecology
Yodo Sugishita, Lingbo Meng, Yuki Suzuki-Takahashi, Sandy Nishimura, Sayako Furuyama, Atsushi Uekawa, Akiko Tozawa-Ono, Junko Migitaka-Igarashi, Tomoe Koizumi, Hibiki Seino, Yasunori Natsuki, Manabu Kubota, Junki Koike, Keisuke Edashige, Nao Suzuki
Summary: After thawing/warming, at least 60 minutes of diffusion washing in media was necessary to significantly reduce the residual cryoprotectants in ovarian tissues frozen by slow freezing or vitrification.
HUMAN REPRODUCTION
(2022)
Article
Biochemistry & Molecular Biology
Mengying Wang, Plamen Todorov, Wanxue Wang, Evgenia Isachenko, Gohar Rahimi, Peter Mallmann, Vladimir Isachenko
Summary: This study found that freezing spermatozoa leads to more down-regulation of genes, which is related to apoptosis changes and immune response. Vitrification induces minor biological changes in human spermatozoa compared to conventional freezing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Reproductive Biology
Patrycja Kurowska, Ewa Mlyczynska, Julia Wajda, Konrad Krol, Karolina Pich, Patrycja Guzman, Aleksandra Greggio, Oliwia Szkraba, Malgorzata Opydo, Joelle Dupont, Agnieszka Rak
Summary: This study investigated the expression of PNX-14 and GPR173 in ovarian tissue and the effects of PNX-14 on granulosa cell function. The results showed that PNX-14 can influence ovarian follicle growth by promoting granulosa cell proliferation, hormone secretion, and cell cycle progression.
REPRODUCTIVE BIOLOGY
(2024)
Article
Reproductive Biology
Sonia Srivastava, Swarnima Mishra, Gyan Babu, Banalata Mohanty
Summary: This study aimed to investigate the efficacy of PD149163 in preventing/ameliorating LPS-induced inflammation in the female reproductive system of mice. The results showed that PD149163 reduced/prevented the inflammation in the ovary and uterine horn, restored the balance of inflammatory cytokines and oxidative stress, and recovered the levels of reproductive hormones and corticosterone.
REPRODUCTIVE BIOLOGY
(2024)
Article
Reproductive Biology
Chen Zhang, Hongyan Cheng, Xue Ye, Heng Cui, Yi Li, Honglan Zhu, Xiaohong Chang
Summary: Extracellular matrix protein 1 (ECM1) is significantly upregulated in endometriosis tissues. Blocking ECM1 attenuates cell migration and invasion. ECM1 regulates cell migration, invasion, and cytoskeleton reorganization via the RhoC/ROCK1 signaling pathway.
REPRODUCTIVE BIOLOGY
(2024)
