Article
Reproductive Biology
P. S. P. Gupta, Kalpana Kaushik, P. Johnson, Kavya Krishna, S. Nandi, S. Mondal, J. Nikhil Kumar Tej, B. Somoskoi, S. Cseh
Summary: The viability of preantral follicles was negatively affected by the low concentration of cryoprotectants in vitrification treatment B, while treatment A and C showed no significant difference compared to the control group. The expression of the apoptotic gene BAK was higher in treatment B, while the development competence genes GDF-9 and BMP-15 showed higher expression in treatment A and C, respectively. The NOBOX gene expression was significantly increased in preantral follicles with treatment B.
Article
Agriculture, Dairy & Animal Science
Bence Somoskoi, Lilla Bordas, Fusa Uno, Dora Kispal, Linda Muller, Dora Torok, Sandor Cseh
Summary: The present study aimed to compare the effects of different cryopreservation methods (closed vs open vitrification) on the survival and developmental rate of canine isolated preantral follicles (PAFs). The results showed that the OPS method provided superior survival rate, in vitro growth, and hormonal production compared to the CT method. This is the first study to compare cryopreservation protocols for canine isolated preantral follicles.
ANIMAL REPRODUCTION SCIENCE
(2023)
Article
Biology
Kalpana Kaushik, P. S. P. Gupta, P. Johnson, Kavya Krishna, S. Nandi, S. Mondal, J. Nikhil Kumar Tej, Somoskoi Bence, Sandor Cseh
Summary: Supplementation of retinol in the vitrification solution can enhance the viability of vitrified sheep preantral follicles and affect the expression of apoptosis and development-related genes.
Article
Multidisciplinary Sciences
Conghui Tian, Lingxiao Shen, Chenjia Gong, Yunxia Cao, Qinghua Shi, Gang Zhao
Summary: This study achieved low-concentration cryopreservation of preantral follicles by using microencapsulation and nanowarming. The research found that this method improved the viability of preantral follicles and maintained their ability to form cavities, compared to conventional water bath warming. Furthermore, it showed no significant impact on in vitro fertilization and embryo transfer.
NATURE COMMUNICATIONS
(2022)
Article
Reproductive Biology
I. P. Silva, D. C. C. Brito, T. E. S. Silva, R. F. Silva, M. I. F. Guedes, J. Y. G. Silva, A. P. R. Rodrigues, R. R. Santos, J. R. Figueiredo
Summary: This study evaluated the effects of ZEN and ENL, alone or in combination, on the survival and morphology of in vitro cultured ovarian preantral follicles. The results showed that while ZEN impaired the morphology of follicles, ENL improved the cultured secondary follicles. However, the combination of ENL with ZEN impaired the quality of primary and secondary follicles.
Article
Reproductive Biology
Lilla Bordas, Bence Somoskoi, Dora Torok, Boglarka Nora Vincze, Sandor Cseh
Summary: There is growing interest in the application of ovarian preantral follicles in reproductive science. Cryopreservation and in vitro culture of these follicles can support fertility preservation in animals with high genetic value, endangered or zoo animals, and women before anticancer therapy. However, there is currently no standardized freezing or vitrification protocol available. This study aimed to examine the viability of preantral follicles cryopreserved using cryotube freezing or OPS vitrification protocols.
REPRODUCTIVE BIOLOGY
(2023)
Article
Reproductive Biology
Hongyu Liu, Ayman Mesalam, Myeong-Don Joo, Shimin Zhang, Lianguang Xu, Jun Wang, Kyeong-Lim Lee, Seok-Hwan Song, Yu-Guo Yuan, Wenfa Lu, Il-Keun Kong
Summary: The study demonstrated the importance of fibronectin in bovine preantral follicle development and revealed that fibronectin could neutralize the deleterious consequences of kisspeptin, suggesting a potential role in the regulation of the PI3K/Sirt1 signaling pathway in bovine preantral follicle development.
Article
Reproductive Biology
Juliana Z. Almeida, L. A. Vieira, C. Maside, A. C. A. Ferreira, N. A. R. Sa, H. H. Correia, V. R. Araujo, R. S. Raposo, J. Smitz, C. C. Campello, J. R. Figueiredo, R. B. Oria
Summary: 5FU does not affect follicular morphology but detrimentally affects follicular growth, estradiol production, and oocyte maturation in isolated ovarian follicles. Concentrations of 5FU greater than 3 mM reduce estradiol levels.
Review
Reproductive Biology
Regina Lucia dos Santos Silva, Ricassio de Sousa Barberino, Maria Helena Tavares de Matose
Summary: The in vitro culture of ovarian preantral follicles allows studying follicular and oocyte growth, using immature oocytes as a potential source for fertilization and screening of ovarian toxic substances. However, oxidative stress caused by reactive oxygen species (ROS) accumulation poses a challenge to the success of the culture system. The addition of antioxidant agents to the culture medium can minimize the damage caused by ROS, supporting follicular survival and development, and producing mature oocytes suitable for fertilization.
Article
Oncology
Juliana Z. Almeida, Laritza F. Lima, Luis A. Vieira, Carolina Maside, Anna C. A. Ferreira, Valdevane R. Araujo, Ana B. G. Duarte, Ramon S. Raposo, Sonia N. Bao, Claudio C. Campello, Luiz F. S. Oliveira, Thayse P. Costa, Jose Garcia Abreu, Jose R. Figueiredo, Reinaldo B. Oria
Summary: The study found that 5-FU can adversely affect the ovarian follicle reserve by reducing the survival of preantral follicles, leading to a significantly lower percentage of normal follicles of all types and impacting the survival of cultured normal primordial follicles.
CANCER CHEMOTHERAPY AND PHARMACOLOGY
(2021)
Article
Veterinary Sciences
Duygu Baki Acar, Muhammed Kursad Birdane, Cigdem Tokyol, Nermin Nuket Gocmen Karabekir, Hatice Murvet Hayran, Erhan Ozenc, Fatma Aktepe, Ebubekir Yazici, Ilknur Pir Yagci, Mehmet Ucar
Summary: This study evaluated the quantitative and morphometric analyses of follicles in Anatolian water buffaloes at different stages of the oestrous cycle. The results showed significant differences in the number of follicles and the ultrastructural characteristics between the luteal and follicular stages.
TURKISH JOURNAL OF VETERINARY & ANIMAL SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Tayebeh Dahmardeh, Mohammad Hossein Ghanian, Bita Ebrahimi
Summary: This study evaluated the potential of a hydrogel composed of thiolated hyaluronic acid (HA-SH) for ovine preantral follicle development compared to alginate hydrogel (ALG). The results showed that HA-SH hydrogel provided better support for follicle growth and had advantages in gene expression levels.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Reproductive Biology
S. Kehoe, K. Jewgenow, P. R. Johnston, B. C. Braun
Summary: The study reveals that early ovarian follicles of domestic cats express a variety of sex hormone and gonadotropin receptors, indicating their potential role in in vitro growth processes.
BIOLOGY OF REPRODUCTION
(2022)
Article
Agriculture, Dairy & Animal Science
D. A. Cardone, A. R. R. Caceres, M. A. Sanhueza, F. A. Bruna, M. R. Laconi
Summary: Heat stress has a significant impact on the number and size of bovine primordial follicles, as well as the number of granulosa cells and the rate of follicle degeneration. These findings provide valuable insights into the effects of heat stress on the reproductive performance of cattle.
Article
Andrology
Sanaz Alaee, Raheleh Asadollahpour, Abasalt Hosseinzadeh Colagar, Tahereh Talaei-Khozani
Summary: The study utilized a decellularized ovary scaffold for the encapsulation and development of preantral follicles. The results indicated that follicles cultured in 3D conditions had significantly higher survival rate, antrum formation rate, E2 and P4 concentrations, and oocyte maturation rate compared to 2D conditions, while gene expression levels showed no significant differences across different culture conditions.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2021)