Efficient production of transgene-free, gene-edited carrot plants via protoplast transformation

Advances in Genome Editing With CRISPR Systems and Transformation Technologies for Plant DNA Manipulation

(2021) Satya Swathi Nadakuduti et al.   Frontiers in Plant Science

Inhibition of Carotenoid Biosynthesis by CRISPR/Cas9 Triggers Cell Wall Remodelling in Carrot

(2021) Tomasz Oleszkiewicz et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Base editing with high efficiency in allotetraploid oilseed rape by A3A‐PBE system

(2020) Hongtao Cheng et al.   PLANT BIOTECHNOLOGY JOURNAL

Establishment of a PEG-mediated protoplast transformation system based on DNA and CRISPR/Cas9 ribonucleoprotein complexes for banana

(2020) Shaoping Wu et al.   BMC PLANT BIOLOGY

Simultaneous targeting of duplicated genes in Petunia protoplasts for flower color modification via CRISPR-Cas9 ribonucleoproteins

(2020) Jihyeon Yu et al.   PLANT CELL REPORTS

Which Factors Affect the Occurrence of Off-Target Effects Caused by the Use of CRISPR/Cas: A Systematic Review in Plants

(2020) Dominik Modrzejewski et al.   Frontiers in Plant Science

CRISPR/Cas9-Mediated Multiply Targeted Mutagenesis in Orange and Purple Carrot Plants

(2019) Zhi-Sheng Xu et al.   MOLECULAR BIOTECHNOLOGY

Regeneration of Solanum tuberosum plants from protoplasts induces widespread genome instability

(2019) Michelle Fossi et al.   PLANT PHYSIOLOGY

CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination

(2019) Teng-Kuei Huang et al.   PLANT CELL REPORTS

Changing Carrot Color: Insertions in DcMYB7 Alter the Regulation of Anthocyanin Biosynthesis and Modification

(2019) Zhi-Sheng Xu et al.   PLANT PHYSIOLOGY

Application of Cas12a and nCas9-activation-induced cytidine deaminase for genome editing and as a non-sexual strategy to generate homozygous/multiplex edited plants in the allotetraploid genome of tobacco

(2019) Chen-Tran Hsu et al.   PLANT MOLECULAR BIOLOGY

Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single-cell mutation detection to mutant plant regeneration

(2018) Choun-Sea Lin et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient CRISPR/Cas9-based genome editing in carrot cells

(2018) Magdalena Klimek-Chodacka et al.   PLANT CELL REPORTS

Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

(2018) Yuan Zong et al.   NATURE BIOTECHNOLOGY

CRISPR–Cas9-mediated genome editing in apple and grapevine

(2018) Yuriko Osakabe et al.   Nature Protocols

Base editing: precision chemistry on the genome and transcriptome of living cells

(2018) Holly A. Rees et al.   NATURE REVIEWS GENETICS

Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing

(2018) Xu Tang et al.   PLANT BIOTECHNOLOGY JOURNAL

A FRET sensor for live-cell imaging of MAP kinase activity in Arabidopsis

(2018) Najia Zaman et al.   PLANT JOURNAL

Generation of Targeted Point Mutations in Rice by a Modified CRISPR/Cas9 System

(2017) Jingying Li et al.   Molecular Plant

Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts

(2016) Mariette Andersson et al.   PLANT CELL REPORTS

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

Improving cold storage and processing traits in potato through targeted gene knockout

(2015) Benjamin M. Clasen et al.   PLANT BIOTECHNOLOGY JOURNAL

Point Mutations in Centromeric Histone Induce Post-zygotic Incompatibility and Uniparental Inheritance

(2015) Sundaram Kuppu et al.   PLoS Genetics

Overexpression of a tobacco osmotin gene in carrot (Daucus carota L.) enhances drought tolerance

(2014) Ali Annon et al.   IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

(2013) Vladimir Nekrasov et al.   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Response of carrot protoplasts and protoplast-derived aggregates to selection using a fungal culture filtrate of Alternaria radicina

(2013) Ewa Grzebelus et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Progress in plant protoplast research

(2013) Tom Eeckhaut et al.   PLANTA

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Production of ketocarotenoids in transgenic carrot plants with an enhanced level of β-carotene

(2011) Mi-Jeong Ahn et al.   Plant Biotechnology Reports

An improved protocol for plant regeneration from leaf- and hypocotyl-derived protoplasts of carrot

(2011) Ewa Grzebelus et al.   PLANT CELL TISSUE AND ORGAN CULTURE

Enhanced disease resistance in transgenic carrot (Daucus carota L.) plants over-expressing a rice cationic peroxidase

(2010) O. Wally et al.   PLANTA

Expression of rabies virus G protein in carrots (Daucus carota)

(2009) Edith Rojas-Anaya et al.   TRANSGENIC RESEARCH