Article
Plant Sciences
Tomasz Oleszkiewicz, Michal Kruczek, Rafal Baranski
Summary: This study demonstrates that the accumulation of carotenoids in carrot cells is affected by nitrogen concentration and the ratio of nitrate to ammonium, while other components in the media do not significantly impact carotenoid content or callus growth.
Article
Plant Sciences
Chandler M. Meyer, Irwin L. Goldman, Ewa Grzebelus, Patrick J. Krysan
Summary: This study presents a method for efficiently producing transgene-free, gene-edited carrot plants. The approach involves using PEG-mediated transformation and protoplast culture to induce targeted mutations, resulting in a large number of transgene-free, gene-edited carrot plants.
PLANT CELL REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Joo Tae Hwang, Hye Jin Kim, Jin Ah Ryuk, Dong Ho Jung, Byoung Seob Ko
Summary: This study explored the medicinal value of carrot leaves and isolated six flavone glycosides from them. A method using NMR and HPLC/UV was developed for their quantitation. Enzymatic deglycosylation of the flavone glycosides was assessed, and the enzyme-treated carrot leaves showed higher inhibitory effects on TNF-a and IL-2 expression. These findings highlight the importance of carrot leaves and provide baseline data for commercial development.
Article
Plant Sciences
Maria Doroteia Campos, Catarina Campos, Amaia Nogales, Helia Cardoso
Summary: The experiment revealed that DcAOX2a plays a dual role in carrot tap root secondary growth and cold stress response, while DcAOX1 shows less correlation. Cold stress generally up-regulated DcAOX2a without consistent effects on DcAOX1, indicating a potential key role of DcAOX2a in the response to stress. The identification of cis-acting regulatory elements at the promoters of both genes showed putative sequences involved in cold stress responsiveness and growth, with DcAOX2a promoter presenting more CAREs related to hormonal pathways than DcAOX1.
Article
Biotechnology & Applied Microbiology
Abraham Palacios-Velasquez, Violeta Quispe-Coquil, Enzo Martin Casimiro-Soriano, Karla Milagros Tapia-Zarate, Alex Ruben Huaman-de la Cruz
Summary: Bioethanol was successfully obtained from carrot residues using a specific enzymatic hydrolysis and fermentation process. The resulting product exhibited superior ethanol content and net calorific value compared to previous studies.
FERMENTATION-BASEL
(2023)
Article
Horticulture
Arthur Domblides, Elena Domblides
Summary: The biodiversity of carrot is important for human diet as it provides useful and necessary components. Eight carrot genotypes with different root colors were distinguished using amplified fragment length polymorphism (AFLP) markers. Three sets of primer pairs produced 92 loci, including 60 polymorphic ones. The genetic distances between the genotypes were calculated using Nei's coefficient. The AFLP method can be used for genetic diversity assessment in carrot breeding accessions with slight modifications including gel staining.
Article
Chemistry, Applied
Bruno Stefani Esposto, Sabrina Guarda Botelho Pinho, Marcelo Thomazini, Ana Paula Ramos, Delia Rita Tapia-Blacido, Milena Martelli-Tosi
Summary: This study aimed to extract bioactive compounds from carrot by-products and evaluated their chemical stability after encapsulation in chitosan-coated TPP-Ch particles. The results showed that the encapsulation efficiency for carotenoids and total phenolic compounds reached over 75%. Furthermore, the TPP-Ch particles demonstrated better stability for carotenoids in various experimental conditions, making them suitable for application in the food industry and aqueous matrices.
Article
Plant Sciences
Maria Belen Perez, Sofia Carvajal, Vanesa Beretta, Florencia Bannoud, Maria Florencia Fangio, Federico Berli, Ariel Fontana, Maria Victoria Salomon, Roxana Gonzalez, Lucia Valerga, Jorgelina C. Altamirano, Mehtap Yildiz, Massimo Iorizzo, Philipp W. Simon, Pablo F. Cavagnaro
Summary: The present study characterized a diverse collection of purple and non-purple carrot accessions for their concentration of anthocyanins, phenolics, carotenoids, and antioxidant capacity. Significant variation was found among the accessions, with acylated anthocyanins being the most dominant pigment. Purple carrots exhibited higher levels of anthocyanins, phenolics, and antioxidant capacity compared to non-purple carrots. Antioxidant capacity was positively correlated with anthocyanin content and total phenolic content, but not with carotenoids. Certain accessions were identified as potential sources for food dyes or increased nutraceutical value.
Article
Plant Sciences
Petar Mitrovic, Ivica Djalovic, Biljana Kiprovski, Sonja Veljovic Jovanovic, Vojislav Trkulja, Aleksandra Jelusic, Tatjana Popovic
Summary: The study found that infection by Candidatus Phytoplasma solani caused abnormal growth in carrot plants, leading to symptoms such as leaf chlorosis and reddening, affecting antioxidative metabolism and chlorophyll content.
Article
Green & Sustainable Science & Technology
Anna Bonasia, Giulia Conversa, Corrado Lazzizera, Giuseppe Gambacorta, Antonio Elia
Summary: The exploration in the sandy shores of Salterns of Margherita di Savoia revealed the cultivation of local carrot landraces with high nutritional and nutraceutical properties, driven by the pedological conditions of the area. Variability in morpho-qualitative traits among carrot genotypes and the potential of a specific landrace, Carota a punta tonda, as a healthy product rich in phenols and beta-carotene were identified, suggesting the importance of on-farm conservation for biodiversity preservation.
Article
Plant Sciences
Vincenzo D'Amelia, Julien Curaba, Muhammad Ali Abid, Salvatore Esposito, Pablo Cavagnaro, Domenico Carputo, Massimo Iorizzo
Summary: The R2R3 MYB gene DcMYB11 is associated with petiole anthocyanin pigmentation in carrot, and a putative enhancer sequence that increases DcMYB11 activity was discovered. This study provides novel insight into the molecular mechanisms controlling anthocyanin accumulation in carrot and expands our knowledge on cis-regulatory sequences in plants.
Article
Plant Sciences
Josefina Wohlfeiler, Maria Soledad Alessandro, Andres Morales, Pablo Federico Cavagnaro, Claudio Romulo Galmarini
Summary: Carrots have genotype-dependent vernalization requirement, where longer cold exposures lead to increased flowering rates and earlier onset of flowering. However, post-vernalization day length does not significantly affect carrot flowering.
Article
Plant Sciences
Antonio Calabuig-Serna, Ricardo Mir, Paloma Arjona, Jose Maria Segui-Simarro
Summary: In this study, the role of calcium in somatic embryogenesis in carrot was investigated. It was found that high calcium levels and the formation of callose layer are indicators of embryogenesis induction. Ca2+ patterns vary in different embryogenic structures. Modulating intracellular Ca2+ levels can effectively regulate the efficiency of somatic embryogenesis in carrot. Additionally, Ca2+-calmodulin signaling is associated with transcriptional remodeling necessary for embryo progression.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Magdalena Klimek-Chodacka, Miron Gieniec, Rafal Baranski
Summary: The study demonstrates an efficient, recombinant DNA-free, multiplex gene-editing method using gRNA:Cas9 ribonucleoprotein (RNP) complexes delivered directly to plant protoplasts. By forming three RNPs in vitro and delivering them to protoplasts, various editing effects were achieved with high efficiency, showcasing the potential of this method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Genetics & Heredity
Xing Liu, Donghang Zhao, Chenggang Ou, Weidong Hao, Zhiwei Zhao, Feiyun Zhuang
Summary: This study identified and categorized PEBP proteins in carrot, celery, and coriander, and analyzed their evolutionary patterns. The expression analysis in carrot showed broad expression patterns across various tissues, with the FT-like genes upregulated during bolting to flowering. Interestingly, the FT-like genes in carrot displayed different temporal-spatial expression characteristics. This study contributes to our understanding of PEBP proteins and provides a foundation for breeding carrot cultivars with bolting resistance.
FRONTIERS IN GENETICS
(2022)
Article
Horticulture
Dariusz Kadluczka, Malgorzata Czernicka, Elwira Sliwinska, Monika Bieniasz, Katarzyna Mackowska, Anna Kapczynska, Ewa Grzebelus
Summary: The genus Lachenalia J. Jacq. ex Js. Murray (Asparagaceae) consists of 136 species found only in South Africa and Namibia. These plants are being studied for their horticultural potential, with a breeding program focusing on pollen viability and morphology to develop new commercial cultivars. In a comprehensive analysis of selected Lachenalia cultivars, it was found that some showed sterility due to meiotic abnormalities, but three cultivars were identified as suitable pollinizers based on the study results. This research provides valuable information for selecting appropriate donors for cross-pollination in developing new Lachenalia cultivars.
SCIENTIA HORTICULTURAE
(2021)
Article
Biochemistry & Molecular Biology
Monika Halat, Magdalena Klimek-Chodacka, Jagoda Orleanska, Malgorzata Baranska, Rafal Baranski
Summary: The research used electronic circular dichroism spectroscopy to confirm the ribonucleoprotein complex formation of Streptococcus pyogenes Cas9 protein and guide RNA, and determined their characteristic spectra. The study also demonstrated that the spectrum of the complex significantly differed from individual components, and the complex retained biological activity after ECD measurements.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Filip Gazdik, Samuel Magnus, Steven J. Roberts, Rafal Baranski, Jana Cechova, Robert Pokluda, Ales Eichmeier, Dariusz Grzebelus, Miroslav Baranek
Summary: This study aimed to monitor the persistence of Xanthomonas campestris pv. campestris (Xcc) in field soil in central Europe, using different PCR assays. It was found that Xcc can persist in soil for up to two years, and the nested real-time PCR method showed higher sensitivity in detecting Xcc in soil compared to conventional PCR.
Article
Biotechnology & Applied Microbiology
Dariusz Kadluczka, Ewa Grzebelus
Summary: The study revealed the presence of CentDc repeats in the genomes of 26 Daucus species belonging to both Daucus I and II subclades, as well as in one closely related outgroup species. The number of chromosome pairs with CentDc signals varied among different Daucus taxa, but the centromeric localization was conserved. Detailed chromosome measurements in 16 accessions provided insights into the intra- and interspecific karyological relationships among them.
Article
Biochemistry & Molecular Biology
Tomasz Oleszkiewicz, Magdalena Klimek-Chodacka, Michal Kruczek, Kamila Godel-Jedrychowska, Katarzyna Sala, Anna Milewska-Hendel, Maciej Zubko, Ewa Kurczynska, Yiping Qi, Rafal Baranski
Summary: Recent data shows that modifications to the carotenoid biosynthesis pathway in plants can alter the expression of genes that impact the chemical composition of the cell wall. Specifically, the psy2 gene is critical for carotenoid biosynthesis and its knockout blocks carotenogenesis. This study demonstrates that inhibited carotenoid biosynthesis triggers remodeling of the cell wall.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Tomasz Oleszkiewicz, Michal Kruczek, Rafal Baranski
Summary: This study demonstrates that the accumulation of carotenoids in carrot cells is affected by nitrogen concentration and the ratio of nitrate to ammonium, while other components in the media do not significantly impact carotenoid content or callus growth.
Article
Biochemistry & Molecular Biology
Magdalena Klimek-Chodacka, Miron Gieniec, Rafal Baranski
Summary: The study demonstrates an efficient, recombinant DNA-free, multiplex gene-editing method using gRNA:Cas9 ribonucleoprotein (RNP) complexes delivered directly to plant protoplasts. By forming three RNPs in vitro and delivering them to protoplasts, various editing effects were achieved with high efficiency, showcasing the potential of this method.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Iwona Kaminska, Aneta Lukasiewicz, Magdalena Klimek-Chodacka, Olga Dlugosz-Grochowska, Julia Rutkowska, Kamil Szymonik, Rafal Baranski
Summary: This study reveals the salt tolerance mechanisms in carrots, including the physiological response in roots, which involve increased content of osmoprotective substances and antioxidants, as well as enhanced activity of antioxidative enzymes for protection against excessive reactive oxygen species.
SCIENTIFIC REPORTS
(2022)
Article
Plant Sciences
Dariusz Kadluczka, Elwira Sliwinska, Ewa Grzebelus
Summary: This study provides comprehensive knowledge of the pollen morphology and nuclear DNA content in Daucus species, which can contribute to understanding their taxonomic relationships and accurate identification of gene bank accessions.
Article
Agronomy
Dariusz Kadluczka, Ewa Grzebelus
Summary: Fruit morphological and anatomical characteristics are important for the taxonomy of Apiaceae. This study compares the fruit morphology and anatomy of Daucus taxa and closely related non-Daucus species. The results provide valuable insights into the taxonomic relationships among these species.
Article
Biochemistry & Molecular Biology
Kamila Godel-Jedrychowska, Anna Milewska-Hendel, Katarzyna Sala, Rafal Baranski, Ewa Kurczynska
Summary: This study aimed to examine the influence of gold nanoparticles (Au NPs) on somatic embryogenesis (SE) in Arabidopsis thaliana. The results show that Au NPs can alter the direction of cell differentiation and affect the chemical composition of cell walls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Katarzyna Stelmach, Ewa Grzebelus
Summary: In this study, an effective and relatively efficient protocol for protoplast-to-plant regeneration of parsnip via indirect somatic embryogenesis was developed and assessed on various cultivars. The isolation efficiency of protoplasts from source material reached an average of 3.6 x 10(6) cells per g of fresh mass using a mixture of cellulase and pectolyase enzymes. The regenerated plants showed morphological similarity to their donors and no change in ploidy level.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Biotechnology & Applied Microbiology
Magdalena Zaranek, Renee Perez-Perez, Anna Milewska-Hendel, Ewa Grzebelus, Alexander Betekhtin
Summary: By applying a mixture of cellulase, pectolyase, and driselase, a high yield of isolated protoplasts from buckwheat was obtained. Morphogenic callus-derived protoplasts showed a higher yield compared to hypocotyl-derived protoplasts. The protoplasts, embedded in an agarose matrix and cultured in a modified medium, successfully underwent cell cycle re-entry and development, leading to plant regeneration.
PLANT CELL TISSUE AND ORGAN CULTURE
(2023)
Article
Plant Sciences
Magdalena Zaranek, Renee Perez-Perez, Anna Milewska-Hendel, Alexander Betekhtin, Ewa Grzebelus
Summary: This study successfully established a protoplast-to-plant system in Tartary buckwheat, a valuable crop with high nutritional importance. The protoplast isolation approach and supplementation of a culture medium effectively stimulated cell division and further development of protoplast cultures, leading to plant regeneration.
Article
Pharmacology & Pharmacy
Heba Desouky Khlifa, Magdalena Klimek-Chodacka, Rafal Baranski, Michal Combik, Hussein Sayed Taha
BRAZILIAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2020)
