Which Factors Affect the Occurrence of Off-Target Effects Caused by the Use of CRISPR/Cas: A Systematic Review in Plants

DNA-Free Genome Editing: Past, Present and Future

(2019) Janina Metje-Sprink et al.   Frontiers in Plant Science

Deciphering Off-Target Effects in CRISPR-Cas9 through Accelerated Molecular Dynamics

(2019) Clarisse G. Ricci et al.   ACS Central Science

Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering

(2019) Oleg Raitskin et al.   PLoS One

Single and multiple gene knockouts by CRISPR–Cas9 in maize

(2019) Nicolas M. Doll et al.   PLANT CELL REPORTS

Genome Editing in Agriculture: Technical and Practical Considerations

(2019) Julia Jansing et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Corrigendum: An EU Perspective on Biosafety Considerations for Plants Developed by Genome Editing and Other New Genetic Modification Techniques (nGMs)

(2019) Michael F. Eckerstorfer et al.   Frontiers in Bioengineering and Biotechnology

High-Resolution Analysis of the Efficiency, Heritability, and Editing Outcomes of CRISPR/Cas9-Induced Modifications of NCED4 in Lettuce ( Lactuca sativa )

(2018) Lien D. Bertier et al.   G3-Genes Genomes Genetics

Functional analysis of the TM6 MADS-box gene in the octoploid strawberry by CRISPR/Cas9-directed mutagenesis

(2018) Carmen Martín-Pizarro et al.   JOURNAL OF EXPERIMENTAL BOTANY

CRISPR/Cas precision: do we need to worry about off-targeting in plants?

(2018) Florian Hahn et al.   PLANT CELL REPORTS

Assessment of genomic changes in a CRISPR/Cas9 Phaeodactylum tricornutum mutant through whole genome resequencing

(2018) Monia Teresa Russo et al.   PeerJ

CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants

(2017) Hao Liu et al.   Molecular Plant

Enhanced proofreading governs CRISPR–Cas9 targeting accuracy

(2017) Janice S. Chen et al.   NATURE

Significant enhancement of fatty acid composition in seeds of the allohexaploid, Camelina sativa , using CRISPR/Cas9 gene editing

(2017) Wen Zhi Jiang et al.   PLANT BIOTECHNOLOGY JOURNAL

Increased efficiency of targeted mutagenesis by CRISPR/Cas9 in plants using heat stress

(2017) Chantal LeBlanc et al.   PLANT JOURNAL

Generation of a Collection of Mutant Tomato Lines Using Pooled CRISPR Libraries

(2017) Thomas B. Jacobs et al.   PLANT PHYSIOLOGY

Characteristics of Genome Editing Mutations in Cereal Crops

(2017) Changfu Zhu et al.   TRENDS IN PLANT SCIENCE

Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes

(2017) Zhen Liang et al.   Nature Communications

CRISPR/Cas9-induced Targeted Mutagenesis and Gene Replacement to Generate Long-shelf Life Tomato Lines

(2017) Qing-hui Yu et al.   Scientific Reports

Methods for Optimizing CRISPR-Cas9 Genome Editing Specificity

(2016) Josh Tycko et al.   MOLECULAR CELL

CRISPR-Cas9-mediated efficient directed mutagenesis and RAD51-dependent and RAD51-independent gene targeting in the mossPhyscomitrella patens

(2016) Cécile Collonnier et al.   PLANT BIOTECHNOLOGY JOURNAL

Regulatory hurdles for genome editing: process- vs. product-based approaches in different regulatory contexts

(2016) Thorben Sprink et al.   PLANT CELL REPORTS

Genome editing in maize directed by CRISPR–Cas9 ribonucleoprotein complexes

(2016) Sergei Svitashev et al.   Nature Communications

Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA

(2016) Yi Zhang et al.   Nature Communications

Highly specific targeted mutagenesis in plants using Staphylococcus aureus Cas9

(2016) Hidetaka Kaya et al.   Scientific Reports

Nucleosomes Inhibit Cas9 Endonuclease Activity in Vitro

(2015) John M. Hinz et al.   BIOCHEMISTRY

Targeted genome modifications in soybean with CRISPR/Cas9

(2015) Thomas B Jacobs et al.   BMC BIOTECHNOLOGY

DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

(2015) Je Wook Woo et al.   NATURE BIOTECHNOLOGY

Designed nucleases for targeted genome editing

(2015) Junwon Lee et al.   PLANT BIOTECHNOLOGY JOURNAL

A Cas9-guide RNA complex preorganized for target DNA recognition

(2015) F. Jiang et al.   SCIENCE

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool

(2015) Manuel Stemmer et al.   PLoS One

CRISPR-P: A Web Tool for Synthetic Single-Guide RNA Design of CRISPR-System in Plants

(2014) Yang Lei et al.   Molecular Plant

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing

(2014) Tessa G. Montague et al.   NUCLEIC ACIDS RESEARCH

Multigene Knockout Utilizing Off-Target Mutations of the CRISPR/Cas9 System in Rice

(2014) Masaki Endo et al.   PLANT AND CELL PHYSIOLOGY

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

(2013) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

Synthetic nucleases for genome engineering in plants: prospects for a bright future

(2013) Holger Puchta et al.   PLANT JOURNAL

Molecular Spectrum of Somaclonal Variation in Regenerated Rice Revealed by Whole-Genome Sequencing

(2011) Akio Miyao et al.   PLANT AND CELL PHYSIOLOGY

The Rate and Molecular Spectrum of Spontaneous Mutations in Arabidopsis thaliana

(2010) S. Ossowski et al.   SCIENCE