Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas

An expanded CRISPRi toolbox for tunable control of gene expression in Pseudomonas putida

(2020) Christos Batianis et al.   Microbial Biotechnology

Complex photonic response reveals three-dimensional self-organization of structural coloured bacterial colonies

(2020) Lukas Schertel et al.   Journal of the Royal Society Interface

Photoswitchable gRNAs for Spatiotemporally Controlled CRISPR-Cas-Based Genomic Regulation

(2020) Elena V. Moroz-Omori et al.   ACS Central Science

The cell organization underlying structural colour is involved in Flavobacterium IR1 predation

(2020) Raditijo Hamidjaja et al.   ISME Journal

Accelerated genome engineering of Pseudomonas putida by I-Sce I―mediated recombination and CRISPR-Cas9 counterselection

(2019) Nicolas T. Wirth et al.   Microbial Biotechnology

Combination of ssDNA recombineering and CRISPR-Cas9 for Pseudomonas putida KT2440 genome editing

(2019) Zhixin Wu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Adaptation and application of a two-plasmid inducible CRISPR-Cas9 system in Clostridium beijerinckii

(2019) Mamou Diallo et al.   METHODS

Controlling CRISPR-Cas9 with ligand-activated and ligand-deactivated sgRNAs

(2019) Kale Kundert et al.   Nature Communications

RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium

(2019) Inés C. Cañadas et al.   ACS Synthetic Biology

Novel ribozymes: discovery, catalytic mechanisms, and the quest to understand biological function

(2019) Christina E Weinberg et al.   NUCLEIC ACIDS RESEARCH

Pathogen-induced activation of disease-suppressive functions in the endophytic root microbiome

(2019) Víctor J. Carrión et al.   SCIENCE

Catalytic RNA, ribozyme, and its applications in synthetic biology

(2019) Soyeon V. Park et al.   BIOTECHNOLOGY ADVANCES

Genome editing and transcriptional repression in Pseudomonas putida KT2440 via the type II CRISPR system

(2018) Jun Sun et al.   Microbial Cell Factories

Genetic manipulation of structural color in bacterial colonies

(2018) Villads Egede Johansen et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Markerless genome editing in Clostridium beijerinckii using the CRISPR-Cpf1 system

(2018) Jie Zhang et al.   JOURNAL OF BIOTECHNOLOGY

Riboregulated toehold-gated gRNA for programmable CRISPR–Cas9 function

(2018) Ka-Hei Siu et al.   Nature Chemical Biology

A Multiplex Genome Editing Method for Escherichia coli Based on CRISPR-Cas12a

(2018) Xiang Ao et al.   Frontiers in Microbiology

A two-plasmid inducible CRISPR/Cas9 genome editing tool for Clostridium acetobutylicum

(2017) François Wasels et al.   JOURNAL OF MICROBIOLOGICAL METHODS

Aptazyme-embedded guide RNAs enable ligand-responsive genome editing and transcriptional activation

(2017) Weixin Tang et al.   Nature Communications

Characterizing a thermostable Cas9 for bacterial genome editing and silencing

(2017) Ioannis Mougiakos et al.   Nature Communications

CRISPR/Cas9-Based Counterselection Boosts Recombineering Efficiency in Pseudomonas putida

(2017) Tomás Aparicio et al.   Biotechnology Journal

Development of Light-Activated CRISPR Using Guide RNAs with Photocleavable Protectors

(2016) Piyush K. Jain et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9

(2016) Dongdong Zhao et al.   Microbial Cell Factories

A chemical-inducible CRISPR–Cas9 system for rapid control of genome editing

(2016) Kaiwen Ivy Liu et al.   Nature Chemical Biology

Consequences of Cas9 cleavage in the chromosome ofEscherichia coli

(2016) Lun Cui et al.   NUCLEIC ACIDS RESEARCH

Next Generation Prokaryotic Engineering: The CRISPR-Cas Toolkit

(2016) Ioannis Mougiakos et al.   TRENDS IN BIOTECHNOLOGY

Rapid and Efficient One-Step Metabolic Pathway Integration in E. coli

(2016) Marcelo C. Bassalo et al.   ACS Synthetic Biology

Coupling the CRISPR/Cas9 System with Lambda Red Recombineering Enables Simplified Chromosomal Gene Replacement in Escherichia coli

(2015) Michael E. Pyne et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Multigene Editing in the Escherichia coli Genome via the CRISPR-Cas9 System

(2015) Yu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

A split-Cas9 architecture for inducible genome editing and transcription modulation

(2015) Bernd Zetsche et al.   NATURE BIOTECHNOLOGY

Photoactivatable CRISPR-Cas9 for optogenetic genome editing

(2015) Yuta Nihongaki et al.   NATURE BIOTECHNOLOGY

Small molecule–triggered Cas9 protein with improved genome-editing specificity

(2015) Kevin M Davis et al.   Nature Chemical Biology

Development of an intein-mediated split–Cas9 system for gene therapy

(2015) Dong-Jiunn Jeffery Truong et al.   NUCLEIC ACIDS RESEARCH

Development of a Markerless Deletion System for the Fish-Pathogenic Bacterium Flavobacterium psychrophilum

(2015) Esther Gómez et al.   PLoS One

Synthetic riboswitches — A tool comes of age

(2014) Florian Groher et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Bacterial group I introns: mobile RNA catalysts

(2014) Georg Hausner et al.   Mobile DNA

A Decade of Riboswitches

(2013) Alexander Serganov et al.   CELL

RNA-guided editing of bacterial genomes using CRISPR-Cas systems

(2013) Wenyan Jiang et al.   NATURE BIOTECHNOLOGY

Inability of Prevotella bryantii to Form a Functional Shine-Dalgarno Interaction Reflects Unique Evolution of Ribosome Binding Sites in Bacteroidetes

(2011) Tomaž Accetto et al.   PLoS One

Mechanism for gene control by a natural allosteric group I ribozyme

(2011) A. G. Y. Chen et al.   RNA

Group I introns: Moving in new directions

(2010) Henrik Nielsen et al.   RNA Biology

An Allosteric Self-Splicing Ribozyme Triggered by a Bacterial Second Messenger

(2010) E. R. Lee et al.   SCIENCE

Riboswitches and the RNA World

(2010) R. R. Breaker   Cold Spring Harbor Perspectives in Biology

RecBCD Enzyme and the Repair of Double-Stranded DNA Breaks

(2008) M. S. Dillingham et al.   MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS

Novobiocin inhibits the self-splicing of the primary transcripts of T4 phage thymidylate synthase gene

(2008) Wi Su Jung et al.   MOLECULAR AND CELLULAR BIOCHEMISTRY