Article
Food Science & Technology
Mark M. Skinner, Jared T. Seale, Maranda S. Cantrell, Joseph M. Collins, Matthew W. Turner, Owen M. McDougal
Summary: This experimental study aimed to detect acrylamide in French fries using methods suitable for the food processing industry, comparing the pros and cons of each method to provide decision-making suggestions for food processors.
Article
Chemistry, Applied
Clemente Ortiz-Romero, Rocio Rios-Reina, Diego L. Garcia-Gonzalez, Maria Jose Cardador, Raquel M. Callejon, Lourdes Arce
Summary: This study aimed to evaluate the potential of analytical techniques for classifying and predicting different categories of olive oil. The results showed that infrared spectroscopy (MIR and NIR) and head-space gas chromatography coupled to an ion mobility spectrometer (HS-GC-IMS) had high classification success rates, with HS-GC-IMS showing greater potential.
Article
Food Science & Technology
Sha Yan, Xuan Wang, Minghui Sun, Wenqian Wang, Liming Wu, Xiaofeng Xue
Summary: Royal jelly is a popular nutraceutical with unique lipid composition, including phospholipids and trans-10-hydroxy-2-decenoic acid. This study found that the lipid composition of royal jelly is influenced by its botanical origin.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Biology
Dinesh Kumar, Pawan Kumar, Kumar Vikram, Himmat Singh
Summary: The study investigates the bio-efficacy of Pimenta dioica leaf derived silver nanoparticles (Pd@AgNPs) against malaria, filarial, and dengue vectors. The synthesized Pd@AgNPs showed larvicidal activity against the larvae of Aedes aegypti, Anopheles stephensi, and Culex quinquefasciatus without affecting non-targeted organisms. The green synthesis of AgNPs offers an effective technique for mosquito control and other therapeutic applications.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Kangyi Zhang, Lingling Gao, Can Zhang, Tao Feng, Haining Zhuang
Summary: A rapid and accurate method for detecting volatile components of corn was established using GC-IMS and GC-MS. The fingerprints of corns under different treatments were analyzed, and key flavor compounds were identified. The corns were classified into four categories, consistent with the results of sensory evaluation. Steamed, boiled, and fried corns were found to be more popular.
FRONTIERS IN CHEMISTRY
(2022)
Article
Food Science & Technology
Shi Nie, Laihao Li, Yueqi Wang, Yanyan Wu, Chunsheng Li, Shengjun Chen, Yongqiang Zhao, Di Wang, Huan Xiang, Ya Wei
Summary: This study identified and quantified volatile organic compounds (VOCs) in fermented sea bass using gas chromatography-ion mobility mass spectrometry (GC-IMS) and gas chromatography-mass spectrometry (GC-MS). Aldehydes were found to be the principal contributors to the overall flavor of fermented sea bass, and several characteristic VOCs were identified. GC-IMS was demonstrated to rapidly detect VOCs, providing a theoretical basis for studying flavor formation in fermented sea bass.
Article
Food Science & Technology
Zewei Zhang, Hongwu Ji, Di Zhang, Shucheng Liu, Xiaoshan Zheng
Summary: In this study, the role of amino acids in the formation of volatile substances during the drying process of Penaeus vannamei was investigated. The results showed that the type and content of volatile components significantly increased in samples with moisture contents between 30% and 5%, and basic amino acids (Arg, Lys, and His) had a strong correlation with the formation of pyrazines.
Article
Chemistry, Analytical
Travis M. Falconer, Birgit Schneider, Carsten Baessmann, Karin Wendt, Artem Filipenko
Summary: The use of trapped ion mobility spectrometry (TIMS) in conjunction with liquid chromatography (LC) and electrospray ionization tandem mass spectrometry (MS/MS) was explored for the analysis of PDE-5 inhibitor analogs. Six out of eight compounds were resolved by LC and TIMS, while the remaining two isomers were distinguished by unique product ions in the MS/MS spectrum. The LC separation corresponded to differences in substitution on the piperazine moiety of the PDE-5 inhibitors, while the TIMS separation corresponded to the position of a nitrogen atom in the fused ring region of the molecules.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Food Science & Technology
Haiyan Yu, Wei Guo, Tong Xie, Lianzhong Ai, Huaixiang Tian, Chen Chen
Summary: The study indicates that traditional Huangjiu produced around Winter Solstice contains more flavor volatiles and has better aroma quality compared to those produced during other periods, making it more popular among consumers.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Keabetswe Masike, Maria A. Stander, Andre de Villiers
Summary: Ion mobility spectrometry (IMS) plays a significant role in natural product analysis by enhancing the quality of mass spectral data, discriminating structurally similar secondary metabolites, and serving as an additional identification criterion.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Multidisciplinary
Emeline Hanozin, Benoit Mignolet, Jonathan Martens, Giel Berden, Damien Sluysmans, Anne-Sophie Duwez, J. Fraser Stoddart, Gauthier Eppe, Jos Oomens, Edwin De Pauw, Denis Morsa
Summary: The text discusses the use of non-dissociative electron transfer as a reductive stimulus in the gas phase to study the conformational transitions of a donor-acceptor oligorotaxane foldamer. By comparing collision cross section distributions and infrared absorption bands of closed-shell and radical molecular ions, the study sheds light on their formation energetics and changes in intramolecular organization as the foldamer becomes more compact, suggesting the emergence of radical-pairing interactions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Agriculture, Multidisciplinary
Johannes Wellmann, Conny Traenkner, Nicolas Dostert, Esther-Corinna Schwarze, Silke Hillebrand, Jakob P. Ley, Peter Winterhalter
Summary: A workflow for comprehensive semi-polar metabolite profiling was developed and validated using liquid chromatography coupled with electrospray ionization ion mobility quadrupole time-of-flight mass spectrometry. This workflow has the capability to distinguish different cultivars of Hydrangea macrophylla ssp. serrata and obtain a list of metabolites responsible for the differentiation. Suspect screening of phenyldihydroisocoumarins provides valuable information for the biosynthesis of phyllodulcin.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jake Breen, Mahin Hashemihedeshi, Roshanak Amiri, Frank L. Dorman, Karl J. Jobst
Summary: Gas chromatography multiplexed with cyclic ion mobility mass spectrometry is a comprehensive two-dimensional separation technique that can resolve compounds that would otherwise coelute in a single-dimension separation. The present study demonstrates a method to mitigate wrap-around in cyclic ion mobility, allowing for non-targeted interpretation of experiments while maintaining the benefits of peak capacities.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Karolina Matyjaszczyk-Gwarda, Agnieszka Kij, Mariola Olkowicz, Benedikt Fels, Kristina Kusche-Vihrog, Maria Walczak, Stefan Chlopicki
Summary: A novel LC-SRM/MS-based method was developed for accurate profiling of glycosaminoglycans (GAGs), providing important information on the status of the endothelial glycocalyx in cell culture and in vivo conditions.
Article
Biochemical Research Methods
Aleksandra Antevska, Connor C. Long, Samuel D. Dupuy, J. Jason Collier, Michael D. Karlstad, Thanh D. Do
Summary: We used liquid chromatography coupled with ion mobility spectrometry-mass spectrometry to investigate the effects of corticosterone on mouse pancreatic islet hormones. Our results show that insulin 1 (Ins1) and insulin 2 (Ins2) have distinct structures and degradation rates. Ins2 may exist in both T and R states, while Ins1 may not. The differences in structure and degradation account for their unique characteristics and the diabetes risk associated with Ins1. Additionally, we observed an increase in the ratio of amylin to Ins1 in corticosterone-treated mice, which correlated with beta-cell proliferation.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Anna Olerinyova, Adar Sonn-Segev, Joseph Gault, Cedric Eichmann, Johannes Schimpf, Adrian H. Kopf, Lucas S. P. Rudden, Dzmitry Ashkinadze, Radoslaw Bomba, Lukas Frey, Jason Greenwald, Matteo T. Degiacomi, Ralf Steinhilper, J. Antoinette Killian, Thorsten Friedrich, Roland Riek, Weston B. Struwe, Philipp Kukura
Summary: This study explores the application of mass photometry (MP) to Integral membrane proteins (IMPs) and membrane-mimetic systems at the single-particle level. By applying MP to amphipathic vehicles and lipid nanodiscs, the research characterizes particle size, sample purity, and heterogeneity, enabling high-resolution studies of membrane-protein structure and interactions. Evidence suggests that using native styrenemaleic acid nanodiscs could reveal new insights into the structure of membrane proteins compared to conventional detergent purification methods.
Article
Chemistry, Physical
Maike Lettow, Kim Greis, Marko Grabarics, Jan Horlebein, Rebecca L. Miller, Gerard Meijer, Gert von Helden, Kevin Pagel
Summary: Glycosaminoglycans (GAGs) play vital roles in biological processes and their characterization, particularly chondroitin and dermatan sulfate, poses challenges due to their varying sulfation patterns. Combining mass spectrometry and cryogenic infrared spectroscopy, researchers have identified that the degree and position of sulfate groups impact the conformational landscape in gas phase, providing insights for future sequencing approaches.
JOURNAL OF PHYSICAL CHEMISTRY A
(2021)
Article
Chemistry, Multidisciplinary
Juliane Brun, Snezana Vasiljevic, Bevin Gangadharan, Mario Hensen, Anu Chandran, Michelle L. Hill, J. L. Kiappes, Raymond A. Dwek, Dominic S. Alonzi, Weston B. Struwe, Nicole Zitzmann
Summary: The study demonstrates the differences in site-specific glycosylation between virus-derived spikes, wild-type non-stabilized spikes, and recombinant engineered spike glycoproteins. Additionally, it shows that their unique cellular secretion pathways lead to distinct protein glycosylation and secretion patterns, including shedding of spike monomeric subunits for the non-stabilized wild-type spike, potentially impacting the resulting immune response and vaccine design.
ACS CENTRAL SCIENCE
(2021)
Article
Biochemical Research Methods
David J. Harvey, Weston B. Struwe, Anna-Janina Behrens, Snezana Vasiljevic, Max Crispin
Summary: This paper discusses the structural determination of N-glycans using negative ion collision-induced dissociation, exploring the formation and fragmentation of different types of multiply charged ions with particular emphasis on the presence of diagnostic fragments in the CID spectra and how these ions can be used to characterize these glycans.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Siyun Chen, Di Wu, Carol Robinson, Weston B. Struwe
Summary: The heterogeneity of glycoproteins poses a major challenge in structural biology, but the combination of native mass spectrometry with glycan exoglycosidase sequencing offers remarkable details of glycoprotein structures. This approach significantly improves the ability to characterize protein glycosylation and address the need for structural biology, opening up new avenues for understanding glycoprotein function.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Richard Karlsson, Pradeep Chopra, Apoorva Joshi, Zhang Yang, Sergey Y. Vakhrushev, Thomas Mandel Clausen, Chelsea D. Painter, Gergo P. Szekeres, Yen-Hsi Chen, Daniel R. Sandoval, Lars Hansen, Jeffrey D. Esko, Kevin Pagel, Douglas P. Dyer, Jeremy E. Turnbull, Henrik Clausen, Geert-Jan Boons, Rebecca L. Miller
Summary: The study integrated various approaches to reveal differences in 3-O-sulfation profiles and their impact on cellular functions, providing new insights for tailored synthesis of next-generation heparins.
Article
Biochemistry & Molecular Biology
Yao Meng, Sami Sanlidag, Sacha A. Jensen, Sean A. Burnap, Weston B. Struwe, Andreas H. Larsen Section, Xinyi Feng, Shruti Mittal, Mark S. P. Sansom, Cecilia Sahlgren, Penny A. Handford
Summary: The extracellular C2 domain of the Jagged/Serrate and Delta families of transmembrane ligands is important for optimal activation of the Notch receptor. Mutations affecting an N-glycosylation site in the C2 domain of JAGGED1 have been found in tumors, reducing the ligand's ability to activate Notch. Site-specific glycan analysis showed that a complex-type or high-mannose N-glycan at this site is required for full Notch activation. The presence of the N-glycan limits the orientation of JAG1 relative to the membrane, facilitating Notch binding.
Article
Multidisciplinary Sciences
Nikolas Hundt, Daniel Cole, Max F. Hantke, Jack J. Miller, Weston B. Struwe, Philipp Kukura
Summary: This study uses mass photometry to investigate the polymerization process of actin and finds that the traditional nucleation-based models cannot explain the observed distribution of actin oligomers. Instead, the key step of filament formation is a slow transition between different states of an actin filament mediated by cation exchange or ATP hydrolysis. These findings have important implications for understanding the mechanism of actin nucleation and studying protein assembly at the molecular level.
Article
Chemistry, Multidisciplinary
Tayyaba Younas, Chang Liu, Weston B. Struwe, Philipp Kukura, Lizhong He
Summary: Researchers have engineered light-responsive RNA-protein nanowires that exhibit non-covalent light-switching ability. By utilizing the high affinity between RNA and protein, they replaced green fluorescence protein with an optogenetic protein pair system, allowing blue light-controlled photo-switching. These RNA-protein structures can serve as biocompatible frameworks for incorporating versatile elements such as RNA, DNA, and enzymes.
Article
Chemistry, Multidisciplinary
Tarick J. El-Baba, Corinne A. Lutomski, Sean A. Burnap, Jani R. Bolla, Lindsay A. Baker, Andrew J. Baldwin, Weston B. Struwe, Carol V. Robinson
Summary: In this study, the impact of glycans on mediating ACE2 dimerization and interactions with Spike was investigated. The researchers found that glycans play a regulatory role in ACE2 dimerization and that positive cooperativity drives ACE2 to complex with multiple Spike trimers. These findings are important for developing strategies to neutralize the virus.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Sean A. Burnap, Weston B. Struwe
Summary: This study demonstrates how proline substitutions used for spike stabilization in SARS-CoV-2 vaccine design directly impact ACE2 receptor interactions. Conformational changes and ACE2 binding are influenced by spike variants and temperature, but are independent of site-specific N-glycosylation.
CHEMICAL COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Fabian Soltermann, Weston B. Struwe, Philipp Kukura
Summary: This paper reviews recent advances in optical technologies for label-free in vitro measurements of protein-protein interactions, including an overview and comparison of existing techniques, as well as discussions on their advantages, limitations, and recent applications.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Chemistry, Physical
Gergo Peter Szekeres, Szilvia Krekic, Rebecca L. Miller, Mark Mero, Kevin Pagel, Zsuzsanna Heiner
Summary: The study revealed that in the presence of Ca2+ ions, chondroitin sulfate interacts with dipalmitoyl phosphatidylcholine mainly affecting the head groups and head-group-bound water molecules, while the organization of phospholipid tails remains mostly unchanged. The potential of VSFG in studying biomolecular interactions at low physiological concentrations was exemplified by observations at a CS concentration below 200 nM. The VSFG spectra imply that CS molecules are organized into ordered macromolecular superstructures with a chiral secondary structure.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
