CRISPR/Cas9 in lepidopteran insects: Progress, application and prospects

Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Protein 9 Mediated Knockout Reveals Functions of the yellow-y Gene in Spodoptera litura

(2021) Xiao-Long Liu et al.   Frontiers in Physiology

20E-mediated regulation of BmKr-h1 by BmKRP promotes oocyte maturation

(2021) Zidan Zhu et al.   BMC BIOLOGY

Genome editing of the SfABCC2 gene confers resistance to Cry1F toxin from Bacillus thuringiensis in Spodoptera frugiperda

(2021) Ming-hui JIN et al.   Journal of Integrative Agriculture

Deep Sequencing Reveals the Comprehensive CRISPR-Cas9 Editing Spectrum in Bombyx mori

(2021) Sanyuan Ma et al.   CRISPR Journal

Functional validation of nicotinic acetylcholine receptor (nAChR) α6 as a target of spinosyns in Spodoptera exigua utilizing the CRISPR/Cas9 system

(2020) Yayun Zuo et al.   PEST MANAGEMENT SCIENCE

CRISPR/Cas9-Mediated Vitellogenin Receptor Knockout Leads to Functional Deficiency in the Reproductive Development of Plutella xylostella

(2020) Lu Peng et al.   Frontiers in Physiology

CRISPR/Cas9-induced vitellogenin knockout lead to incomplete embryonic development in Plutella xylostella

(2020) Ming-Min Zou et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

CRISPR ‐mediated gene knockout reveals nicotinic acetylcholine receptor ( nAChR ) subunit α6 as a target of spinosyns in Helicoverpa armigera

(2020) Jing Wang et al.   PEST MANAGEMENT SCIENCE

miR-34 regulates larval growth and wing morphogenesis by directly modulating ecdysone signalling and cuticle protein in Bombyx mori

(2020) Zulian Liu et al.   RNA Biology

Functional redundancy of two ABC transporter proteins in mediating toxicity of Bacillus thuringiensis to cotton bollworm

(2020) Jing Wang et al.   PLoS Pathogens

CRISPR-Mediated Knockout of the ABCC2 Gene in Ostrinia furnacalis Confers High-Level Resistance to the Bacillus thuringiensis Cry1Fa Toxin

(2020) Xingliang Wang et al.   Toxins

An Orphan Pheromone Receptor Affects the Mating Behavior of Helicoverpa armigera

(2020) Song Cao et al.   Frontiers in Physiology

Genome editing in the fall armyworm, Spodoptera frugiperda: Multiple sgRNA/Cas9 method for identification of knockouts in one generation

(2020) Guan-Heng Zhu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Down-regulation of lysosomal protein ABCB6 increases gossypol susceptibility in Helicoverpa armigera

(2020) Minghui Jin et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

A single ortholog of teashirt and tiptop regulates larval pigmentation and adult appendage patterning in Bombyx mori

(2020) Ru Zhang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Evaluation of five candidate receptors for three Bt toxins in the beet armyworm using CRISPR-mediated gene knockouts

(2020) Jianlei Huang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Resistance to Bacillus thuringiensis Cry1Ac toxin requires mutations in two Plutella xylostella ATP-binding cassette transporter paralogs

(2020) Zhaoxia Liu et al.   PLoS Pathogens

The Cadherin Protein Is Not Involved in Susceptibility to Bacillus thuringiensis Cry1Ab or Cry1Fa Toxins in Spodoptera frugiperda

(2020) Jianfeng Zhang et al.   Toxins

CRISPR/Cas9‐based functional analysis of yellow gene in the diamondback moth, Plutella xylostella

(2020) Yajun Wang et al.   Insect Science

ABC transporter mutations in C ry 1F ‐resistant fall armyworm ( Spodoptera frugiperda ) do not result in altered susceptibility to selected small molecule pesticides

(2020) Heba Abdelgaffar et al.   PEST MANAGEMENT SCIENCE

Directed Evolution of CRISPR/Cas Systems for Precise Gene Editing

(2020) Rongming Liu et al.   TRENDS IN BIOTECHNOLOGY

Ommochrome pathway genes kynurenine 3-hydroxylase and cardinal participate in eye pigmentation in Plutella xylostella

(2020) Xuejiao Xu et al.   BMC Molecular and Cell Biology

Functional conservation and diversification of yellow-y in lepidopteran insects

(2020) Yu Shirai et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

The receptor tyrosine kinase torso regulates ecdysone homeostasis to control developmental timing in Bombyx mori

(2020) Zhong‐Jie Zhang et al.   Insect Science

Gene editing the BmNPV inhibitor of apoptosis protein 2 (iap2) as an antiviral strategy in transgenic silkworm

(2020) Liang Huang et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Two ABC transporters are differentially involved in the toxicity of two Bacillus thuringiensis Cry1 toxins to the invasive crop‐pest Spodoptera frugiperda (J. E. Smith)

(2020) Minghui Jin et al.   PEST MANAGEMENT SCIENCE

RNAi and CRISPR/Cas9 as Functional Genomics Tools in the Neotropical Stink Bug, Euschistus heros

(2020) Deise Cagliari et al.   Insects

A Δ9 desaturase (SlitDes11) is associated with the biosynthesis of ester sex pheromone components in Spodoptera litura

(2019) Ya-Nan Zhang et al.   PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY

CRISPR/Cas9-mediated ebony knockout results in puparium melanism in Spodoptera litura

(2019) Hong-Lun Bi et al.   Insect Science

CRISPR/Cas9 Initiated Transgenic Silkworms as a Natural Spinner of Spider Silk

(2019) Xiaoli Zhang et al.   BIOMACROMOLECULES

CRISPR/Cas9-mediated knockout of both the PxABCC2 and PxABCC3 genes confers high-level resistance to Bacillus thuringiensis Cry1Ac toxin in the diamondback moth, Plutella xylostella (L.)

(2019) Zhaojiang Guo et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

microRNA‐2738 regulates gene expression in the sex determination pathway in Bombyx mori

(2019) Zu‐Lian Liu et al.   Insect Science

Maelstrom regulates spermatogenesis of the silkworm, Bombyx mori

(2019) Kai Chen et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

BmSd gene regulates the silkworm wing size by affecting the Hippo pathway

(2019) Jin Yin et al.   Insect Science

Mutation of the seminal protease gene, serine protease 2, results in male sterility in diverse lepidopterans

(2019) Xia Xu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Mutation of doublesex induces sex-specific sterility of the diamondback moth Plutella xylostella

(2019) Yaohui Wang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

CRISPR Disruption of BmOvo Resulted in the Failure of Emergence and Affected the Wing and Gonad Development in the Silkworm Bombyx mori

(2019) Honglun Bi et al.   Insects

Construction and application of an HSP70 promoter-inducible genome editing system in transgenic silkworm to induce resistance to Nosema bombycis

(2019) Zhanqi Dong et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

The silkworm (Bombyx mori) neuropeptide orcokinin is involved in the regulation of pigmentation

(2019) Pingyang Wang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Disruption of the ovarian serine protease ( Osp ) gene causes female sterility in Bombyx mori and Spodoptera litura

(2019) Xia Xu et al.   PEST MANAGEMENT SCIENCE

Knockout of the HaREase Gene Improves the Stability of dsRNA and Increases the Sensitivity of Helicoverpa armigera to Bacillus thuringiensis Toxin

(2019) Ruobing Guan et al.   Frontiers in Physiology

Interplay between Developmental Flexibility and Determinism in the Evolution of Mimetic Heliconius Wing Patterns

(2019) Carolina Concha et al.   CURRENT BIOLOGY

CRISPR/Cas9 mediated gene knockout reveals a more important role of PBP1 than PBP2 in the perception of female sex pheromone components in Spodoptera litura

(2019) Guan-Heng Zhu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Disruption of nicotinic acetylcholine receptor α6 mediated by CRISPR/Cas9 confers resistance to spinosyns in Plutella xylostella

(2019) Xingliang Wang et al.   PEST MANAGEMENT SCIENCE

An insulin-like growth factor-like peptide promotes ovarian development in the silkmoth Bombyx mori

(2019) Daiki Fujinaga et al.   Scientific Reports

Identification of yellow gene family in Agrotis ipsilon and functional analysis of Aiyellow-y by CRISPR/Cas9

(2018) Xi'en Chen et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

MicroRNA-14 regulates larval development time in Bombyx mori

(2018) Zulian Liu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Genome editing in Bombyx mori : New opportunities for silkworm functional genomics and the sericulture industry

(2018) San-Yuan Ma et al.   Insect Science

BmHpo mutation induces smaller body size and late stage larval lethality in the silkworm, Bombyx mori

(2018) Xia Xu et al.   Insect Science

A palmitoyltransferase Approximated gene Bm-app regulates wing development in Bombyx mori

(2018) Ye Yu et al.   Insect Science

Identification and functional characterization of doublesex gene in the testis of Spodoptera litura

(2018) Qian Du et al.   Insect Science

A highly specific SpCas9 variant is identified by in vivo screening in yeast

(2018) Antonio Casini et al.   NATURE BIOTECHNOLOGY

RNA interference technology in crop protection against arthropod pests, pathogens and nematodes

(2018) Moises Zotti et al.   PEST MANAGEMENT SCIENCE

Bombyx mori histone methyltransferase BmAsh2 is essential for silkworm piRNA-mediated sex determination

(2018) Zhiqian Li et al.   PLoS Genetics

Disruption of sex-specific doublesex exons results in male- and female-specific defects in the black cutworm, Agrotis ipsilon

(2018) Xien Chen et al.   PEST MANAGEMENT SCIENCE

CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in fall armyworm moth (Spodoptera frugiperda)

(2018) Ke Wu et al.   PLoS One

Body Shape and Coloration of Silkworm Larvae Are Influenced by a Novel Cuticular Protein

(2017) Gao Xiong et al.   GENETICS

Genetic Basis of Melanin Pigmentation in Butterfly Wings

(2017) Linlin Zhang et al.   GENETICS

CRISPR/Cas9 mediated G4946E substitution in the ryanodine receptor of Spodoptera exigua confers high levels of resistance to diamide insecticides

(2017) Yayun Zuo et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Depletion of juvenile hormone esterase extends larval growth in Bombyx mori

(2017) Zhongjie Zhang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Deletion of the Bombyx mori odorant receptor co-receptor ( BmOrco ) impairs olfactory sensitivity in silkworms

(2017) Qun Liu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Sexually dimorphic traits in the silkworm, Bombyx mori , are regulated by doublesex

(2017) Jun Xu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

CRISPR/Cas9 mediated genome editing of Helicoverpa armigera with mutations of an ABC transporter gene HaABCA2 confers resistance to Bacillus thuringiensis Cry2A toxins

(2017) Jing Wang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Knockout of a P-glycoprotein gene increases susceptibility to abamectin and emamectin benzoate in Spodoptera exigua

(2017) Y.-Y. Zuo et al.   INSECT MOLECULAR BIOLOGY

The FOXO transcription factor controls insect growth and development by regulating juvenile hormone degradation in the silkworm,Bombyx mori

(2017) Baosheng Zeng et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

CRISPR/Cas9 Editing of the Codling Moth (Lepidoptera: Tortricidae) CpomOR1 Gene Affects Egg Production and Viability

(2017) Stephen F. Garczynski et al.   JOURNAL OF ECONOMIC ENTOMOLOGY

Tissue-specific genome editing of laminA/C in the posterior silk glands of Bombyx mori

(2017) Yuanyuan Liu et al.   Journal of Genetics and Genomics

CRISPR/Cas9 in insects: Applications, best practices and biosafety concerns

(2017) Clauvis Nji Tizi Taning et al.   JOURNAL OF INSECT PHYSIOLOGY

CRISPR/Cas9 mediated BLOS2 knockout resulting in disappearance of yellow strips and white spots on the larval integument in Spodoptera litura

(2017) Guan-Heng Zhu et al.   JOURNAL OF INSECT PHYSIOLOGY

Transgenic Clustered Regularly Interspaced Short Palindromic Repeat/Cas9-Mediated Viral Gene Targeting for Antiviral Therapy of Bombyx mori Nucleopolyhedrovirus

(2017) Shuqing Chen et al.   JOURNAL OF VIROLOGY

Bombyx mori P-element Somatic Inhibitor (BmPSI) Is a Key Auxiliary Factor for Silkworm Male Sex Determination

(2017) Jun Xu et al.   PLoS Genetics

Body Shape and Coloration of Silkworm Larvae Are Influenced by a Novel Cuticular Protein

(2017) Gao Xiong et al.   GENETICS

Genetic Basis of Melanin Pigmentation in Butterfly Wings

(2017) Linlin Zhang et al.   GENETICS

Establishment of a highly efficient virus-inducible CRISPR/Cas9 system in insect cells

(2016) Zhan-Qi Dong et al.   ANTIVIRAL RESEARCH

Functional characterization of SlitPBP3 in Spodoptera litura by CRISPR/Cas9 mediated genome editing

(2016) Guan-Heng Zhu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Identification of the silkworm quail gene reveals a crucial role of a receptor guanylyl cyclase in larval pigmentation

(2016) Masashi Yuasa et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

Functional validation of cadherin as a receptor of Bt toxin Cry1Ac in Helicoverpa armigera utilizing the CRISPR/Cas9 system

(2016) Jing Wang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in the global pest, diamondback moth (Plutella xylostella)

(2016) Yuping Huang et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

CRISPR/Cas9-mediated targeted gene mutagenesis inSpodoptera litura

(2016) Hong-Lun Bi et al.   Insect Science

20-Hydroxyecdysone (20E) Primary Response GeneE75Isoforms Mediate Steroidogenesis Autoregulation and Regulate Developmental Timing inBombyx

(2016) Kang Li et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Molecular logic behind the three-way stochastic choices that expand butterfly colour vision

(2016) Michael Perry et al.   NATURE

Heritable genome editing with CRISPR/Cas9 induces anosmia in a crop pest moth

(2016) Fotini A. Koutroumpa et al.   Scientific Reports

Functional analysis of Bombyx Wnt1 during embryogenesis using the CRISPR/Cas9 system

(2015) Zhongjie Zhang et al.   JOURNAL OF INSECT PHYSIOLOGY

MiR-2 family targetsawdandfngto regulate wing morphogenesis inBombyx mori

(2015) Lin Ling et al.   RNA Biology

Genome-Scale CRISPR-Mediated Control of Gene Repression and Activation

(2014) Luke A. Gilbert et al.   CELL

Highly efficient multiplex targeted mutagenesis and genomic structure variation in Bombyx mori cells using CRISPR/Cas9

(2014) Yuanyuan Liu et al.   INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY

CRISPR-Cas systems for editing, regulating and targeting genomes

(2014) Jeffry D Sander et al.   NATURE BIOTECHNOLOGY

Efficient chromosomal gene modification with CRISPR/cas9 and PCR-based homologous recombination donors in cultured Drosophila cells

(2014) R. Bottcher et al.   NUCLEIC ACIDS RESEARCH

The CRISPR/Cas System mediates efficient genome engineering in Bombyx mori

(2013) Yueqiang Wang et al.   CELL RESEARCH

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

RNA-guided complex from a bacterial immune system enhances target recognition through seed sequence interactions

(2011) B. Wiedenheft et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design

(2010) Olle Terenius et al.   JOURNAL OF INSECT PHYSIOLOGY

Sequence- and Structure-Specific RNA Processing by a CRISPR Endonuclease

(2010) Rachel E. Haurwitz et al.   SCIENCE

CRISPR/Cas, the Immune System of Bacteria and Archaea

(2010) P. Horvath et al.   SCIENCE

Structural Basis for DNase Activity of a Conserved Protein Implicated in CRISPR-Mediated Genome Defense

(2009) Blake Wiedenheft et al.   STRUCTURE

A Novel Family of Sequence-specific Endoribonucleases Associated with the Clustered Regularly Interspaced Short Palindromic Repeats

(2008) Natalia Beloglazova et al.   JOURNAL OF BIOLOGICAL CHEMISTRY