CRISPR‐based platform for rapid, sensitive and field‐deployable detection of scale drop disease virus in Asian sea bass ( Lates calcarifer )

Machine Learning-Driven and Smartphone-Based Fluorescence Detection for CRISPR Diagnostic of SARS-CoV-2

(2021) Aubin Samacoits et al.   ACS Omega

An engineered CRISPR-Cas12a variant and DNA-RNA hybrid guides enable robust and rapid COVID-19 testing

(2021) Kean Hean Ooi et al.   Nature Communications

Development of cross‐priming amplification (CPA) combined with colorimetric and lateral flow dipstick visualization for scale drop disease virus (SDDV) detection

(2021) Terawut Prasitporn et al.   JOURNAL OF FISH DISEASES

An enhanced method for nucleic acid detection with CRISPR-Cas12a using phosphorothioate modified primers and optimized gold-nanopaticle strip

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A sensitive and specific SYBR Green-based qPCR assay for detecting scale drop disease virus (SDDV) in Asian sea bass

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An isothermal method for sensitive detection of Mycobacterium tuberculosis complex using CRISPR/Cas12a cis- and trans-cleavage

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CRISPR–Cas12-based detection of SARS-CoV-2

(2020) James P. Broughton et al.   NATURE BIOTECHNOLOGY

Draft genome sequence of scale drop disease virus (SDDV) retrieved from metagenomic investigation of infected barramundi, Lates calcarifer (Bloch, 1790)

(2020) Pattanapon Kayansamruaj et al.   JOURNAL OF FISH DISEASES

Rapid (30-second), equipment-free purification of nucleic acids using easy-to-make dipsticks

(2020) Michael G. Mason et al.   Nature Protocols

Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA

(2020) Maturada Patchsung et al.   Nature Biomedical Engineering

Potential application of CRISPR-Cas12a fluorescence assay coupled with rapid nucleic acid amplification for detection of white spot syndrome virus in shrimp

(2019) Thawatchai Chaijarasphong et al.   AQUACULTURE

A validated semi-nested PCR for rapid detection of scale drop disease virus (SDDV) in Asian sea bass (Lates calcarifer)

(2019) Onanong Charoenwai et al.   JOURNAL OF VIROLOGICAL METHODS

Cas12aVDet: A CRISPR/Cas12a-Based Platform for Rapid and Visual Nucleic Acid Detection

(2019) Bei Wang et al.   ANALYTICAL CHEMISTRY

Colorimetric detection of scale drop disease virus in Asian sea bass using loop-mediated isothermal amplification with xylenol orange

(2019) Sirintip Dangtip et al.   AQUACULTURE

Rapid, CRISPR-Based, Field-Deployable Detection Of White Spot Syndrome Virus In Shrimp

(2019) Timothy J. Sullivan et al.   Scientific Reports

Cas12a-Based On-Site and Rapid Nucleic Acid Detection of African Swine Fever

(2019) Jing Bai et al.   Frontiers in Microbiology

CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity

(2018) Janice S. Chen et al.   SCIENCE

Multiplexed and portable nucleic acid detection platform with Cas13, Cas12a, and Csm6

(2018) Jonathan S. Gootenberg et al.   SCIENCE

CRISPR-Cas12a-assisted nucleic acid detection

(2018) Shi-Yuan Li et al.   Cell Discovery

Mortality from scale drop disease in farmed Lates calcarifer in Southeast Asia

(2018) Saengchan Senapin et al.   JOURNAL OF FISH DISEASES

Nucleic acid detection with CRISPR-Cas13a/C2c2

(2017) Jonathan S. Gootenberg et al.   SCIENCE

Nucleic acid purification from plants, animals and microbes in under 30 seconds

(2017) Yiping Zou et al.   PLOS BIOLOGY

A Novel Virus Causes Scale Drop Disease in Lates calcarifer

(2015) Ad de Groof et al.   PLoS Pathogens

Equipment-Free Incubation of Recombinase Polymerase Amplification Reactions Using Body Heat

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The pathology of ‘scale drop syndrome’ in Asian seabass, Lates calcarifer Bloch, a first description

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NUPACK: Analysis and design of nucleic acid systems

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