Article
Agronomy
Lea Rako, Arati Agarwal, Linda Semeraro, Adam Broadley, Brendan C. Rodoni, Mark J. Blacket
Summary: The novel Khapra and 18S LAMP assays are able to amplify target beetle DNA quickly and sensitively, even from partially degraded samples, improving speed, accuracy, and confidence of detection at incursion points. The assays are portable and easy to implement in field conditions with limited resources, aiding rapid biosecurity responses in countries affected by Khapra beetle infestations.
PEST MANAGEMENT SCIENCE
(2021)
Review
Materials Science, Multidisciplinary
Nazente Atceken, Muhammad Munzer Alseed, Sajjad Rahmani Dabbagh, Ali K. Yetisen, Savas Tasoglu
Summary: LAMP technology is a fast, efficient, and low-cost diagnostic method for pathogen detection, which can be applied to various bacteria, viruses, and parasitic pathogens. In addition, LAMP technology can also be used for SNP genotyping and mutation detection.
ADVANCED ENGINEERING MATERIALS
(2023)
Article
Environmental Sciences
Qiqi Xue, Lingwei Sheng, Junjie Guo, Minghui Zou, Qiao Teng, Sijia Xu, Yuanyuan Li, Jinhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) method was successfully established for the detection of the Dermatophagoides farinae allergen. This method showed higher sensitivity, specificity, and efficiency compared to conventional PCR. It did not cross-react with other arthropod species commonly found in indoor dust. This study provides a potential model for the rapid detection of allergens produced by other house dust mites in the future.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biophysics
Zhiheng Xu, Kun Yin, Xiong Ding, Ziyue Li, Xuanhao Sun, Baikun Li, Rajesh V. Lalla, Robert Gross, Changchun Liu
Summary: A simple, disposable, and integrated electronic-tube cap (E-tube cap) for DNA detection at the point-of-care was developed and successfully demonstrated the presence of 100 copies of λDNA in saliva samples. The device showed fast, accurate, and cost-effective diagnostic capabilities, making it particularly suitable for point-of-care molecular diagnostics of infectious diseases.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biotechnology & Applied Microbiology
Lauren Waller, Zhilin Guo, Rui Tang, Zunming Zhang, Edward Wang, Jarred Yasuhara-Bell, Louise Laurent, Yu-Hwa Lo
Summary: This study demonstrates a rapid, scalable, high-throughput multiplex diagnostic method capable of detecting multiple gene targets of the SARS-CoV-2 virus in non-laboratory settings.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Wenjuan Wu, Congcong Yin, Aiqin Yue, Jingping Niu, Weijun Du, Dingbin Liu, Jinzhong Zhao
Summary: In this study, a loop-mediated isothermal amplification (LAMP) assay was developed for visual and rapid detection of the major prevalent SMV strain in China. The assay showed high analytical performance and potential for early detection of SMV-SC7-associated plant diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Plant Sciences
Huiying Sun, Lin Sun, Liuliu Yang, Zehao Wang, Zihao Xia, Xinyu Yang, Zhiwei Jiao, Jie Feng, Yue Liang
Summary: A rapid and sensitive detection method based on LAMP technology was successfully developed in this study for accurate detection of the causal agent of sunflower black stem. This method not only has the characteristics of rapid and sensitive detection, but also can be applied to different types of infected tissues and seed samples, which is of great significance for assessing the prevalence and implementing sustainable management of the disease.
Article
Entomology
Wenzhao Yang, Yun Su, Lingyu Zeng, Yue Zhang, Farman Ullah, Xiaoliang Wang, Xiaonan Li, Xiaodong Feng, Zhihong Li
Summary: Bactrocera tsuneonis and Bactrocera minax are damaging pests that have a significant impact on citrus in China, causing financial losses and reducing citrus production. It is difficult to differentiate these two species based on morphological characteristics, but we have developed a rapid and accurate diagnostic tool using DNA barcoding region and LAMP technology to distinguish them.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Food Science & Technology
Nor Asmara Tasrip, Mohd Nasir Mohd Desa, Nur Fadhilah Khairil Mokhtar, Nurhayatie Sajali, Amalia Mohd Hashim, Md. Eaqub Ali, Cheah Yoke Kqueen
Summary: The study successfully detected porcine DNA in gelatin products using porcine-specific LAMP technology, demonstrating excellent specificity, sensitivity, and rapidity.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2021)
Article
Biochemistry & Molecular Biology
Aurore C. Poirier, Dai Kuang, Bianca S. Siedler, Khushboo Borah, Jai W. Mehat, Jialin Liu, Cui Tai, Xiaoli Wang, Arnoud H. M. van Vliet, Wei Ma, David R. Jenkins, John Clark, Roberto M. La Ragione, Jieming Qu, Johnjoe McFadden
Summary: We developed highly sensitive and specific LAMP assays for the clinical identification of K. pneumoniae and detection of carbapenem resistance.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Virology
Eline Meyers, Jeongho Park, Anja Coen, Leen Raman, Stefan Heytens, Joowon Rhee, Elizaveta Padalko, Piet Cools
Summary: During the SARS-CoV-2 pandemic, rapid and sensitive detection of the virus is crucial. A new point-of-care test system based on LAMP technology was evaluated, and it showed high clinical sensitivity and specificity for SARS-CoV-2 detection. The system's easy operation and ready-to-use reagents make it suitable for various settings, contributing to more efficient outbreak control and quarantine policies.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Environmental Sciences
Xiaotian Han, Ting Zhao, Tian Yan, Rencheng Yu
Summary: A LAMP method coupled with a LFD was developed for detecting harmful algae Karenia mikimotoi, showing high sensitivity and specificity, indicating a promising approach for detecting and monitoring harmful algal blooms.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2022)
Article
Multidisciplinary Sciences
Gaolian Xu, Julien Reboud, Yunfei Guo, Hao Yang, Hongchen Gu, Chunhai Fan, Xiaohua Qian, Jonathan M. Cooper
Summary: Accelerating the design of nucleic acid amplification methods is challenging, but necessary for diagnosing diseases. This study presents a generalizable isothermal nucleic acid amplification methodology that can be applied to clinical settings, allowing for the detection of short miRNAs and difficult pathogenic targets.
NATURE COMMUNICATIONS
(2022)
Review
Chemistry, Analytical
Debayan Das, Manaswini Masetty, Aashish Priye
Summary: Paper-based diagnostics offer a cost-effective and portable alternative to traditional diagnostic methods. However, current paper-based serology tests lack the accuracy and sensitivity of molecular diagnostic tests. Loop-mediated isothermal amplification (LAMP) provides PCR-like performance while simplifying the assay complexity. This review discusses the integration of paper microfluidics with LAMP for improved accuracy and versatility in point-of-care testing.
Article
Chemistry, Analytical
Anto J. U. K. John, Peijun J. W. He, Ioannis N. Katis, P. P. Galanis, Alice H. Iles, Robert W. Eason, Collin L. Sones
Summary: With the ongoing SARS-CoV-2 pandemic, the development of rapid and cost-effective diagnostic methods has become crucial. A testing strategy utilizing a sensitive RT-LAMP assay in commercially available glass capillaries has been developed, providing results in a short timeframe and reducing the burden on testing supply chains.
ANALYTICA CHIMICA ACTA
(2021)
Article
Infectious Diseases
Yuttapong Thawornwattana, Ha Thanh Dong, Kornsunee Phiwsaiya, Pakkakul Sangsuriya, Saengchan Senapin, Pakorn Aiewsakun
Summary: Tilapia lake virus (TiLV) is an emerging virus that rapidly spread worldwide over the past 6 years, its origin and epidemiology characteristics are still largely unknown.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Fisheries
Naruporn Rungrueng, Watcharachai Meemetta, Kornsunee Phiwsaiya, Ha Thanh Dong, Wattana Panphut, Saengchan Senapin
Summary: Accuracy is key in PCR diagnostics, with inconsistent results observed when changing Taq DNA polymerase brands. Testing of 6 primer sets for aquaculture viruses showed differences in specificity and sensitivity, highlighting the potential improvement of detection sensitivity by adding ammonium sulfate to reaction buffers.
AQUACULTURE INTERNATIONAL
(2021)
Article
Fisheries
Nguyen Dinh-Hung, Pattiya Sangpo, Thanapong Kruangkum, Pattanapon Kayansamruaj, Tilladit Rung-ruangkijkrai, Saengchan Senapin, Channarong Rodkhum, Ha Thanh Dong
Summary: This study identified the distribution of TiLV in the entire brain of experimentally infected Nile tilapia, showing that the virus was broadly distributed throughout the forebrain and hindbrain. The virus may enter the brain through the circulatory system and induce brain dysfunction. Understanding the pattern of viral localization in the brain could help elucidate the neurological disorders of the diseased fish.
JOURNAL OF FISH DISEASES
(2021)
Article
Zoology
Nukul Saengphan, Bhinyo Panijpan, Saengchan Senapin, Parames Laosinchai, Auaree Suksomnit, Kornsunee Phiwsaiya
Summary: A new species of small freshwater prawn was discovered in the upper southern peninsula of Thailand. This new species, M. prachuapense sp. nov., has distinct characteristics that differentiate it from closely related species, and phylogenetic analysis confirms its unique status.
Article
Fisheries
Terawut Prasitporn, Saengchan Senapin, Akapon Vaniksampanna, Siwaporn Longyant, Parin Chaivisuthangkura
Summary: The study introduced a new method for detecting SDDV virus, utilizing UDG-assisted CPA combined with HNB and LFD technologies, which proved to be rapid, simple, and effective.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Le Thanh Dien, Nguyen Vu Linh, Pattiya Sangpo, Saengchan Senapin, Sophie St-Hilaire, Channarong Rodkhum, Ha Thanh Dong
Summary: The research demonstrated that using NB-O-3 is a safe and effective method to control bacterial diseases in aquaculture, and it significantly improves the survival rate of Nile tilapia against a lethal pathogen challenge.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Thao Thu Mai, Pattanapon Kayansamruaj, Suwimon Taengphu, Saengchan Senapin, Janina Z. Costa, Jorge del-Pozo, Kim D. Thompson, Channarong Rodkhum, Ha Thanh Dong
Summary: The study developed heat-killed and formalin-killed vaccines for prevention of Tilapia tilapinevirus disease, with both vaccines showing significant protection and different gene expressions in the immune response.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Jantana Kampeera, Sirintip Dangtip, Rapheephat Suvannakad, Pakapreud Khumwan, Saengchan Senapin, Wansika Kiatpathomchai
Summary: Tilapia is a globally significant aquaculture species facing mortality issues due to infectious pathogens like TiLV. A novel colorimetric assay based on RT-LAMP and AuNP has been developed for rapid onsite detection, showing high agreement with conventional PCR methods recommended by OIE but requiring significantly less time to perform.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Jerome Delamare-Deboutteville, Suwimon Taengphu, Han Ming Gan, Pattanapon Kayansamruaj, Partho Pratim Debnath, Andrew Barnes, Shaun Wilkinson, Minami Kawasaki, Chadag Vishnumurthy Mohan, Saengchan Senapin, Ha Thanh Dong
Summary: This study successfully demonstrated a rapid diagnostic and genotyping method for tilapia lake virus using a combination of specific PCR assay and ONT-based amplicon sequencing, with high accuracy and operational feasibility. The approach is suitable for deployment in regional aquatic animal health diagnostic laboratories in low- and medium-income countries, allowing for fast identification and genotyping of emerging infectious pathogens from field samples within a single day.
JOURNAL OF FISH DISEASES
(2021)
Article
Fisheries
Thanwarat Sukonta, Saengchan Senapin, Watcharachai Meemetta, Thawatchai Chaijarasphong
Summary: A novel detection platform for SDDV based on CRISPR-Cas12a and recombinase polymerase amplification technology has been developed, showing high sensitivity and specificity for rapid and accurate diagnosis of SDDV. This user-friendly method can be completed within an hour at room temperature, potentially serving as a valuable tool for combating large-scale epidemics in fish farming communities.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Partho Pratim Debnath, Nguyen Dinh-Hung, Suwimon Taengphu, Vuong Viet Nguyen, Jerome Delamare-Deboutteville, Saengchan Senapin, Chadag Vishnumurthy Mohan, Ha Thanh Dong, Channarong Rodkhum
Summary: Sixteen countries have reported the presence of tilapia lake virus (TiLV), an emerging pathogen affecting tilapia. In Bangladesh, 20% of farms tested positive for TiLV in tilapia, while co-cultivated fish species and invertebrates did not show signs of TiLV infection. Experimental infection only showed clinical signs in Nile tilapia, suggesting that species in polycultures may not be susceptible to TiLV.
JOURNAL OF FISH DISEASES
(2022)
Article
Fisheries
Ha Thanh Dong, Pattiya Sangpo, Le Thanh Dien, Thao Thu Mai, Nguyen Vu Linh, Jorge del-Pozo, Krishna R. Salin, Saengchan Senapin
Summary: CAIB in the pancreas of adult Nile tilapia infected with TiPV serves as a diagnostic feature, indicating exclusive replication of TiPV in pancreatic tissues, often accompanied by co-infection with Streptococcus agalactiae and secondary granulomas.
JOURNAL OF FISH DISEASES
(2022)
Review
Fisheries
Ha Thanh Dong, Thawatchai Chaijarasphong, Andrew C. Barnes, Jerome Delamare-Deboutteville, Peiyu Alison Lee, Saengchan Senapin, Chadag Vishnumurthy Mohan, Kathy F. J. Tang, Sharon E. McGladdery, Melba G. Bondad-Reantaso
Summary: The article "Diagnostics for diseases of tilapia: From basic to molecular diagnosis" explores the importance of the three levels of diagnostics for diseases of tilapia, particularly the frequently overlooked basic procedures. The article also covers current and emerging molecular diagnostic technologies for tilapia pathogens, and discusses future developments for transferring these technologies to farmers and stakeholders for a sustainable aquatic food system transformation.
REVIEWS IN AQUACULTURE
(2023)
Article
Fisheries
Ramida Phasunon, Suwimon Taengphu, Wattana Panphut, Satid Chatchaiphan, Ha Thanh Dong, Saengchan Senapin
Summary: A newly developed probe-based qPCR method for the detection of S. iniae was reported in this study. The method demonstrated high specificity and sensitivity, and can be applied for the diagnosis and surveillance of S. iniae infections in fish populations.
JOURNAL OF FISH DISEASES
(2023)
Article
Fisheries
Nguyen Vu Linh, Nareerat Khongcharoen, Dinh-Hung Nguyen, Le Thanh Dien, Naruporn Rungrueng, Chayuda Jhunkeaw, Pattiya Sangpo, Saengchan Senapin, Toungporn Uttarotai, Wattana Panphut, Sophie St-Hilaire, Hien Van Doan, Ha Thanh Dong
Summary: This study investigated the effects of oxygen nanobubble treatment on Nile tilapia. The results showed that both short-term and long-term exposure to high oxygen levels did not have obvious adverse effects on the fish, although there were some changes in the gut microbiome.
FISH & SHELLFISH IMMUNOLOGY
(2023)
Article
Fisheries
Hunter S. Bailey, Ashley N. Fincannon, Lee A. Fuiman
Summary: This study investigated the transfer of fatty acids from broodstock diets to eggs and the time needed for the eggs to equilibrate to the diet in Southern flounder. The findings indicate that different diet treatments significantly affect the fatty acid composition of eggs, and it takes 8-16 weeks for the eggs to adapt to changes in the adult diet. These results are important for improving broodstock diets and feeding protocols in stock-enhancement programs.
Article
Fisheries
Yu Liu, Mingtao Lei, Hector Victor, Yan Wang
Summary: This study confirms the feasibility of replacing raw fish diet with a formulated diet in commercial farming of large yellow croaker, which can increase fish survival and yield, and reduce feed cost and body lipid deposition.
Article
Fisheries
Xiaoyan Zhang, Lize San, Yucong Yang, Yuehong Tao, Jiangong Ren, Yufeng Liu, Zhongwei He, Jiashuo Di, Ziteng Pei, Guixing Wang, Jilun Hou
Summary: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. This study successfully induced gynogenetic families in Thamnaconus modestus and established a complete method for gynogenesis induction.
Article
Fisheries
Julieta C. Martinelli, Megan Considine, Helen R. Casendino, Carolyn M. Tarpey, Isadora Jimenez-Hidalgo, Jacqueline L. Padilla-Gamino, Teri L. King, Lorenz Hauser, Steven Rumrill, Chelsea L. Wood
Summary: Shell-boring polychaetes have caused significant damage to mariculture operations worldwide, particularly in the US Pacific region. This study provides a comprehensive dataset on the prevalence, species distribution, and environmental associations of these parasites, highlighting the impact of season, culturing methods, and environmental factors on infestation.
Article
Fisheries
Juan Gao, Xueshan Li, Kangle Lu, Kai Song, Jian Zhang, Ling Wang, Chunxiao Zhang
Summary: This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog tadpoles. The optimal dietary protein level for bullfrog tadpoles was estimated to be 42.49%-46.50% of the diet.
Article
Fisheries
Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang, Jiong Chen
Summary: This study developed a centrifugal microfluidic chip with on-chip RPA to detect five pathogenic microorganisms. The chip enabled the parallel analysis of six genetic markers from a single sample and allowed for the highly automated detection of multiple samples. Compared with PCR and DNA sequencing, the on-chip RPA assay showed high sensitivity and specificity for detecting multiple pathogens in shrimp aquaculture.
Article
Fisheries
Xingchen Huo, Pengxu Wang, Fengxia Zhao, Qian Liu, Qingqing Tian, Lingjie Tang, Maolin Lv, Zhaohui Wei, Chunrong Yang, Jianguo Su
Summary: Bacterial diseases in aquaculture ponds have negative effects on sustainable development. The use of composite I20 biotherapy has been shown to effectively treat bacterial diseases in bullfrog ponds, providing a new strategy for controlling bacterial diseases in aquaculture.
Article
Fisheries
Peng Yin, Takaya Saito, Per Gunnar Fjelldal, Bjorn Tharandur Bjornsson, Sofie Charlotte Remo, Sandeep Sharma, Rolf Erik Olsen, Kristin Hamre
Summary: This study aims to investigate the effect of seasonal changes in temperature and photoperiod on the redox metabolism of Atlantic salmon at the molecular level. The research findings suggest that the changing photoperiod and temperature influenced the redox metabolism of Atlantic salmon, highlighting the importance of oxidative status in the ecological implications.
Article
Fisheries
Nicholas Oppong Mensah, Jeffery Kofi Asare, Emmanuel Tetteh-Doku Mensah, Ernest Christlieb Amrago, Frank Osei Tutu, Anthony Donkor
Summary: This study investigates aquaculture farmers' preference for climate-smart aquaculture insurance products, the challenges they face, and their preferred insurance coverage. The results show that farmers prefer Climate-Induced Aquaculture Stock Mortality Insurance and the most significant constraint is the delay in claim settlement.
Article
Fisheries
Qiu-Ping Chai, Pei Wu, Wei-Dan Jiang, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Lin Feng, Xiao-Qiu Zhou
Summary: The study found that appropriate levels of potassium diformate (KDF) can enhance the immune defense and mediate the inflammatory process in fish, possibly through the regulation of T cell differentiation via JAK-STAT and NF-kappa B signaling pathways.
Article
Fisheries
Tian Zhu, Haomin Jia, Haopeng Zhang, Yujing Xiao, Cui Han, Jiaxin Yang
Summary: Chicken manure has significant effects on the cultivation of Chlorella and rotifers. The optimal amount of chicken manure extract for continuous cultivation is 10 mLL-1. Chicken manure can promote the growth of Chlorella and improve the fatty acid composition, indirectly enhancing the growth condition and fatty acid content of rotifers.
Article
Fisheries
Zheng Luo, Yang Yu, Zhenning Bao, Fuhua Li
Summary: This study analyzed the heritability and genetic correlation of two growth traits in Pacific white shrimp and evaluated the genomic prediction using different genomic selection models. The results showed that the NeuralNet model had the highest prediction accuracy and better prospects for predicting shrimp growth traits.
Article
Fisheries
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: Supplementing diets with a combination of spice oleoresins can reduce lipid accumulation and inflammation in farmed fish. The combination of spice oleoresins also has an immunomodulatory effect on the fish's intestinal immune system, potentially offering anti-inflammatory benefits.
Article
Fisheries
Beibei Zhao, Renhui Mei, Le Li, Di Hu, Lei Li
Summary: This study reveals that JfCXCL8_L1a and JfCXCL8_L1b have different immune pathways, and JfCXCL8_L1b plays a significant role in enhancing the adaptive immunity of T cell-dependent antigen.
Article
Fisheries
Yuhang Liu, Danying Cao, Nan Wu, Xuyang Zhao, Qingsong Zhu, Lian Su, Fatima Altaf, Qianqian Zhang, Haokun Liu, Yongming Li, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Aihua Li, Yaping Wang, Xiao-Qin Xia
Summary: Based on previous research, sinomenine has been found to protect mucosal immunity in farmed fish species by preventing intestinal pathological changes and regulating gene expression related to inflammation. It also enhances immune homeostasis and controls the growth of pathogenic bacteria.