High level production of diacetylchitobiose deacetylase by refactoring genetic elements and cellular metabolism

Engineering diacetylchitobiose deacetylase from Pyrococcus horikoshii towards an efficient glucosamine production

(2021) Ziyang Huang et al.   BIORESOURCE TECHNOLOGY

Synergistic improvement of N-acetylglucosamine production by engineering transcription factors and balancing redox cofactors

(2021) Chen Deng et al.   METABOLIC ENGINEERING

CAMERS‐B: CRISPR/Cpf1 Assisted Multiple‐genes Editing and Regulation System for Bacillus subtilis

(2020) Yaokang Wu et al.   BIOTECHNOLOGY AND BIOENGINEERING

The elucidation of phosphosugar stress response in Bacillus subtilis guides strain engineering for high N ‐acetylglucosamine production

(2020) Tengfei Niu et al.   BIOTECHNOLOGY AND BIOENGINEERING

Construction of second generation protease‐deficient hosts of Bacillus subtilis for secretion of foreign proteins

(2019) Leizhen Zhao et al.   BIOTECHNOLOGY AND BIOENGINEERING

Secretory expression fine-tuning and directed evolution of diacetylchitobiose deacetylase by Bacillus subtilis

(2019) Zhu Jiang et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Engineering a highly efficient expression system to produce BcaPRO protease in Bacillus subtilis by an optimized promoter and signal peptide

(2019) Yihan Liu et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Bacillus strains as human probiotics: characterization, safety, microbiome, and probiotic carrier

(2019) Na-Kyoung Lee et al.   FOOD SCIENCE AND BIOTECHNOLOGY

Construction of a highly active secretory expression system in Bacillus subtilis of a recombinant amidase by promoter and signal peptide engineering

(2019) Xue-Mei Kang et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

High-yield and plasmid-free biocatalytic production of 5-methylpyrazine-2-carboxylic acid by combinatorial genetic elements engineering and genome engineering of Escherichia coli

(2019) Liuyan Gu et al.   ENZYME AND MICROBIAL TECHNOLOGY

Glucosamine promotes osteoblast proliferation by modulating autophagy via the mammalian target of rapamycin pathway

(2018) Chen Lv et al.   BIOMEDICINE & PHARMACOTHERAPY

Advances and prospects of Bacillus subtilis cellular factories: From rational design to industrial applications

(2018) Yang Gu et al.   METABOLIC ENGINEERING

Evaluation of the anti-inflammatory actions of various functional food materials including glucosamine on synovial cells

(2017) Yoshie Yamagishi et al.   Molecular Medicine Reports

Anti-proliferative potential of Glucosamine in renal cancer cells via inducing cell cycle arrest at G0/G1 phase

(2017) Long-sheng Wang et al.   BMC Urology

Significantly improving the yield of recombinant proteins in Bacillus subtilis by a novel powerful mutagenesis tool (ARTP): Alkaline α-amylase as a case study

(2015) Yingfang Ma et al.   PROTEIN EXPRESSION AND PURIFICATION

Introducing a salt bridge into the lipase of Stenotrophomonas maltophilia results in a very large increase in thermal stability

(2014) Jian-Ping Wu et al.   BIOTECHNOLOGY LETTERS

Expression from engineeredEscherichia colichromosome and crystallographic study of archaealN,N′-diacetylchitobiose deacetylase

(2014) Shouhei Mine et al.   FEBS Journal

Developing Bacillus spp. as a cell factory for production of microbial enzymes and industrially important biochemicals in the context of systems and synthetic biology

(2013) Long Liu et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Construction of a 5′-controllable stabilizing element (CoSE) for over-production of heterologous proteins at high levels in Bacillus subtilis

(2013) Trang Thi Phuong Phan et al.   JOURNAL OF BIOTECHNOLOGY

Extracytoplasmic Proteases Determining the Cleavage and Release of Secreted Proteins, Lipoproteins, and Membrane Proteins in Bacillus subtilis

(2013) Laxmi Krishnappa et al.   JOURNAL OF PROTEOME RESEARCH

Expression, refolding, and purification of active diacetylchitobiose deacetylase from Pyrococcus horikoshii

(2012) Shouhei Mine et al.   PROTEIN EXPRESSION AND PURIFICATION

Simple Cloning via Direct Transformation of PCR Product (DNA Multimer) to Escherichia coli and Bacillus subtilis

(2011) Chun You et al.   APPLIED AND ENVIRONMENTAL MICROBIOLOGY

Improved glucosamine and N-acetylglucosamine production by an engineered Escherichia coli via step-wise regulation of dissolved oxygen level

(2011) Xin Chen et al.   BIORESOURCE TECHNOLOGY

Simple, fast and high-efficiency transformation system for directed evolution of cellulase in Bacillus subtilis

(2010) Xiao-Zhou Zhang et al.   Microbial Biotechnology

Automated design of synthetic ribosome binding sites to control protein expression

(2009) Howard M Salis et al.   NATURE BIOTECHNOLOGY

High-Level Secretion of a Chimeric Thermostable Lichenase from Bacillus subtilis by Screening of Site-Mutated Signal Peptides with Structural Alterations

(2008) Ling-lin Fu et al.   CURRENT MICROBIOLOGY