Single-Neuron RNA Modification Analysis by Mass Spectrometry: Characterizing RNA Modification Patterns and Dynamics with Single-Cell Resolution

Cell culture NAIL-MS allows insight into human tRNA and rRNA modification dynamics in vivo

(2021) Matthias Heiss et al.   Nature Communications

Small RNAs are modified with N-glycans and displayed on the surface of living cells

(2021) Ryan A. Flynn et al.   CELL

Characterization of Neuronal RNA Modifications during Non-associative Learning in Aplysia Reveals Key Roles for tRNAs in Behavioral Sensitization

(2021) Kevin D. Clark et al.   ACS Central Science

Single-cell analysis targeting the proteome

(2020) Mahmoud Labib et al.   Nature Reviews Chemistry

Loss of m1acp3Ψ Ribosomal RNA Modification Is a Major Feature of Cancer

(2020) Artem Babaian et al.   Cell Reports

Enhanced single-cell metabolomics by capillary electrophoresis electrospray ionization-mass spectrometry with field amplified sample injection

(2020) Hsiao-Wei Liao et al.   ANALYTICA CHIMICA ACTA

Biphasic Liquid Microjunction Extraction for Profiling Neuronal RNA Modifications by Liquid Chromatography–Tandem Mass Spectrometry

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Regulation of sleep homeostasis mediator adenosine by basal forebrain glutamatergic neurons

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The RNA degradosome promotes tRNA quality control through clearance of hypomodified tRNA

(2019) Satoshi Kimura et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Lipid Heterogeneity between Astrocytes and Neurons Revealed by Single-Cell MALDI-MS Combined with Immunocytochemical Classification

(2019) Elizabeth K. Neumann et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Single Cell Proteomics by Data-Independent Acquisition To Study Embryonic Asymmetry in Xenopus laevis

(2019) Anumita Saha-Shah et al.   ANALYTICAL CHEMISTRY

The Effects of Ultraviolet Radiation on Nucleoside Modifications in RNA

(2018) Congliang Sun et al.   ACS Chemical Biology

Proteomic Analysis of Single Mammalian Cells Enabled by Microfluidic Nanodroplet Sample Preparation and Ultrasensitive NanoLC-MS

(2018) Ying Zhu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Nano LC-MS using capillary columns enables accurate quantification of modified ribonucleosides at low femtomol levels

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METTL3-mediated m6A modification is required for cerebellar development

(2018) Chen-Xin Wang et al.   PLOS BIOLOGY

Reversible RNA Modification N 1 -methyladenosine (m 1 A) in mRNA and tRNA

(2018) Chi Zhang et al.   GENOMICS PROTEOMICS & BIOINFORMATICS

The Role of m6A/m-RNA Methylation in Stress Response Regulation

(2018) Mareen Engel et al.   NEURON

METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation

(2018) Zeyu Zhang et al.   CELL RESEARCH

m6A facilitates hippocampus-dependent learning and memory through YTHDF1

(2018) Hailing Shi et al.   NATURE

Categorizing Cells on the Basis of their Chemical Profiles: Progress in Single-Cell Mass Spectrometry

(2017) Troy J. Comi et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Detecting RNA modifications in the epitranscriptome: predict and validate

(2017) Mark Helm et al.   NATURE REVIEWS GENETICS

N6-methyladenosine (m6A) recruits and repels proteins to regulate mRNA homeostasis

(2017) Raghu R Edupuganti et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

MODOMICS: a database of RNA modification pathways. 2017 update

(2017) Pietro Boccaletto et al.   NUCLEIC ACIDS RESEARCH

N6-methyladenosine (m6A) recruits and repels proteins to regulate mRNA homeostasis

(2017) Raghu R Edupuganti et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Region-specific RNA m 6 A methylation represents a new layer of control in the gene regulatory network in the mouse brain

(2017) Mengqi Chang et al.   Open Biology

Single Cell Peptide Heterogeneity of Rat Islets of Langerhans

(2016) Erik T. Jansson et al.   ACS Chemical Biology

Determination of DNA and RNA Methylation in Circulating Tumor Cells by Mass Spectrometry

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Experience-Dependent Accumulation of N 6 -Methyladenosine in the Prefrontal Cortex Is Associated with Memory Processes in Mice

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Transcriptome-wide mapping reveals reversible and dynamic N1-methyladenosine methylome

(2016) Xiaoyu Li et al.   Nature Chemical Biology

tRNA base methylation identification and quantification via high-throughput sequencing

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Simultaneous Quantification of Methylated Cytidine and Adenosine in Cellular and Tissue RNA by Nano-Flow Liquid Chromatography–Tandem Mass Spectrometry Coupled with the Stable Isotope-Dilution Method

(2015) Lijuan Fu et al.   ANALYTICAL CHEMISTRY

Attomole quantification and global profile of RNA modifications: Epitranscriptome of human neural stem cells

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Single-cell mass spectrometry reveals small molecules that affect cell fates in the 16-cell embryo

(2015) Rosemary M. Onjiko et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Cytosine methylation of tRNA-Asp by DNMT2 has a role in translation of proteins containing poly-Asp sequences

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Transcriptome in vivo analysis (TIVA) of spatially defined single cells in live tissue

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Aplysia Ganglia Preparation for Electrophysiological and Molecular Analyses of Single Neurons

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Age-associated bidirectional modulation of gene expression in single identified R15 neuron of Aplysia

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tRNA tKUUU, tQUUG, and tEUUC wobble position modifications fine-tune protein translation by promoting ribosome A-site binding

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Single-Cell Metabolomics: Changes in the Metabolome of Freshly Isolated and Cultured Neurons

(2012) Peter Nemes et al.   ACS Chemical Neuroscience

Isotope-Based Analysis of Modified tRNA Nucleosides Correlates Modification Density with Translational Efficiency

(2012) Caterina Brandmayr et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Aplysia

(2011) Leonid L. Moroz   CURRENT BIOLOGY

N6-Methyladenosine in nuclear RNA is a major substrate of the obesity-associated FTO

(2011) Guifang Jia et al.   Nature Chemical Biology

Profiling metabolites and peptides in single cells

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The RNA modification database, RNAMDB: 2011 update

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Do different neurons age differently? Direct genome-wide analysis of aging in single identified cholinergic neurons

(2010) Leonid Moroz   Frontiers in Aging Neuroscience

A Quantitative Systems Approach Reveals Dynamic Control of tRNA Modifications during Cellular Stress

(2010) Clement T. Y. Chan et al.   PLoS Genetics