Therapeutic mRNA Engineering from Head to Tail

Biological implications of decapping: beyond bulk mRNA decay

(2021) Fivos Borbolis et al.   FEBS Journal

The Limitless Future of RNA Therapeutics

(2021) Tulsi Ram Damase et al.   Frontiers in Bioengineering and Biotechnology

High-throughput 5′ UTR engineering for enhanced protein production in non-viral gene therapies

(2021) Jicong Cao et al.   Nature Communications

Stop codon context influences genome-wide stimulation of termination codon readthrough by aminoglycosides

(2020) Jamie R Wangen et al.   eLife

Pseudouridine-mediated stop codon read-through in S. cerevisiae is sequence context-independent

(2020) Hironori Adachi et al.   RNA

Codon Usage and Splicing Jointly Influence mRNA Localization

(2020) Christine Mordstein et al.   Cell Systems

Decoding mRNA translatability and stability from the 5′ UTR

(2020) Longfei Jia et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

RNA Circularization Diminishes Immunogenicity and Can Extend Translation Duration In Vivo

(2019) R. Alexander Wesselhoeft et al.   MOLECULAR CELL

Quality and quantity control of gene expression by nonsense-mediated mRNA decay

(2019) Tatsuaki Kurosaki et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Emerging Evidence of Translational Control by AU-Rich Element-Binding Proteins

(2019) Hiroshi Otsuka et al.   Frontiers in Genetics

Human 5′ UTR design and variant effect prediction from a massively parallel translation assay

(2019) Paul J. Sample et al.   NATURE BIOTECHNOLOGY

Codon bias confers stability to human mRNA s

(2019) Fabian Hia et al.   EMBO REPORTS

Programmable RNA N6-methyladenosine editing by CRISPR-Cas9 conjugates

(2019) Xiao-Min Liu et al.   Nature Chemical Biology

N6-Methyladenosine Modification Controls Circular RNA Immunity

(2019) Y. Grace Chen et al.   MOLECULAR CELL

mRNA structure regulates protein expression through changes in functional half-life

(2019) David M. Mauger et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Mixed tailing by TENT4A and TENT4B shields mRNA from rapid deadenylation

(2018) Jaechul Lim et al.   SCIENCE

Translation Termination and Ribosome Recycling in Eukaryotes

(2018) Christopher U.T. Hellen   Cold Spring Harbor Perspectives in Biology

Translation Elongation and Recoding in Eukaryotes

(2018) Thomas E. Dever et al.   Cold Spring Harbor Perspectives in Biology

Engineering circular RNA for potent and stable translation in eukaryotic cells

(2018) R. Alexander Wesselhoeft et al.   Nature Communications

Translation initiation by cap-dependent ribosome recruitment: Recent insights and open questions

(2018) Nikolay E. Shirokikh et al.   Wiley Interdisciplinary Reviews-RNA

Uridine Depletion and Chemical Modification Increase Cas9 mRNA Activity and Reduce Immunogenicity without HPLC Purification

(2018) Sriram Vaidyanathan et al.   Molecular Therapy-Nucleic Acids

Differential m6A, m6Am, and m1A Demethylation Mediated by FTO in the Cell Nucleus and Cytoplasm

(2018) Jiangbo Wei et al.   MOLECULAR CELL

Author Correction: Functional 5′ UTR mRNA structures in eukaryotic translation regulation and how to find them

(2018) Kathrin Leppek et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

mRNA cap regulation in mammalian cell function and fate

(2018) Alison Galloway et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Improving mRNA-Based Therapeutic Gene Delivery by Expression-Augmenting 3′ UTRs Identified by Cellular Library Screening

(2018) Alexandra G. Orlandini von Niessen et al.   MOLECULAR THERAPY

Circ-ZNF609 Is a Circular RNA that Can Be Translated and Functions in Myogenesis

(2017) Ivano Legnini et al.   MOLECULAR CELL

Ribosome Collision Is Critical for Quality Control during No-Go Decay

(2017) Carrie L. Simms et al.   MOLECULAR CELL

Short poly(A) tails are a conserved feature of highly expressed genes

(2017) Sarah Azoubel Lima et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Short poly(A) tails are a conserved feature of highly expressed genes

(2017) Sarah Azoubel Lima et al.   NATURE STRUCTURAL & MOLECULAR BIOLOGY

Adjacent Codons Act in Concert to Modulate Translation Efficiency in Yeast

(2016) Caitlin E. Gamble et al.   CELL

Structural basis for m7G recognition and 2′-O-methyl discrimination in capped RNAs by the innate immune receptor RIG-I

(2016) Swapnil C. Devarkar et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Rules of UGA-N decoding by near-cognate tRNAs and analysis of readthrough on short uORFs in yeast

(2016) Petra Beznosková et al.   RNA

RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling

(2016) Ann Fiegen Durbin et al.   mBio

pEVL: A Linear Plasmid for Generating mRNA IVT Templates With Extended Encoded Poly(A) Sequences

(2016) Alexandra E Grier et al.   Molecular Therapy-Nucleic Acids

The race to decipher the top secrets of TOP mRNAs

(2015) Oded Meyuhas et al.   Biochimica et Biophysica Acta-Gene Regulatory Mechanisms

Codon Optimality Is a Major Determinant of mRNA Stability

(2015) Vladimir Presnyak et al.   CELL

N 1 -methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice

(2015) Oliwia Andries et al.   JOURNAL OF CONTROLLED RELEASE

Sequence-engineered mRNA Without Chemical Nucleoside Modifications Enables an Effective Protein Therapy in Large Animals

(2015) Andreas Thess et al.   MOLECULAR THERAPY

eIF3 targets cell-proliferation messenger RNAs for translational activation or repression

(2015) Amy S. Y. Lee et al.   NATURE

RNA circularization strategies in vivo and in vitro

(2015) Sonja Petkovic et al.   NUCLEIC ACIDS RESEARCH

Codon-optimized Human Sodium Iodide Symporter (opt-hNIS) as a Sensitive Reporter and Efficient Therapeutic Gene

(2015) Young-Hwa Kim et al.   Theranostics

Quantitative analysis of mammalian translation initiation sites by FACS-seq

(2014) W. L. Noderer et al.   Molecular Systems Biology

Massively parallel functional annotation of 3′ untranslated regions

(2014) Wenxue Zhao et al.   NATURE BIOTECHNOLOGY

Design of an Optimized Wilms’ Tumor 1 (WT1) mRNA Construct for Enhanced WT1 Expression and Improved Immunogenicity In Vitro and In Vivo

(2013) Daphné Benteyn et al.   Molecular Therapy-Nucleic Acids

Statistical Analysis of Readthrough Levels for Nonsense Mutations in Mammalian Cells Reveals a Major Determinant of Response to Gentamicin

(2012) Célia Floquet et al.   PLoS Genetics

Expression of therapeutic proteins after delivery of chemically modified mRNA in mice

(2011) Michael S D Kormann et al.   NATURE BIOTECHNOLOGY

Ribose 2′-O-methylation provides a molecular signature for the distinction of self and non-self mRNA dependent on the RNA sensor Mda5

(2011) Roland Züst et al.   NATURE IMMUNOLOGY

Translation, stability, and resistance to decapping of mRNAs containing caps substituted in the triphosphate chain with BH3, Se, and NH

(2011) W. Su et al.   RNA

Phosphorothioate cap analogs increase stability and translational efficiency of RNA vaccines in immature dendritic cells and induce superior immune responses in vivo

(2010) A N Kuhn et al.   GENE THERAPY

Tandem Stop Codons in Ciliates That Reassign Stop Codons

(2009) Marie Adachi et al.   JOURNAL OF MOLECULAR EVOLUTION

Locked Nucleic Acid (LNA)-Modified Dinucleotide mRNA Cap Analogue: Synthesis, Enzymatic Incorporation, and Utilization

(2009) Anilkumar R. Kore et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Reprogramming of 3′ Untranslated Regions of mRNAs by Alternative Polyadenylation in Generation of Pluripotent Stem Cells from Different Cell Types

(2009) Zhe Ji et al.   PLoS One

Most mammalian mRNAs are conserved targets of microRNAs

(2008) R. C. Friedman et al.   GENOME RESEARCH

Incorporation of Pseudouridine Into mRNA Yields Superior Nonimmunogenic Vector With Increased Translational Capacity and Biological Stability

(2008) Katalin Karikó et al.   MOLECULAR THERAPY