Genome engineering through CRISPR/Cas9 technology in the human germline and pluripotent stem cells

Cas-Designer: a web-based tool for choice of CRISPR-Cas9 target sites

(2015) Jeongbin Park et al.   BIOINFORMATICS

Selective Elimination of Mitochondrial Mutations in the Germline by Genome Editing

(2015) Pradeep Reddy et al.   CELL

Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System

(2015) Bernd Zetsche et al.   CELL

CRISPR-Cas9-Mediated Genetic Screening in Mice with Haploid Embryonic Stem Cells Carrying a Guide RNA Library

(2015) Cuiqing Zhong et al.   Cell Stem Cell

Small Molecules Enhance CRISPR Genome Editing in Pluripotent Stem Cells

(2015) Chen Yu et al.   Cell Stem Cell

Ethics and germline gene editing

(2015) J. Sugarman   EMBO REPORTS

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Stem cells in reproductive medicine: ready for the patient?: Figure 1

(2015) R. Vassena et al.   HUMAN REPRODUCTION

Direct Injection of CRISPR/Cas9-Related mRNA into Cytoplasm of Parthenogenetically Activated Porcine Oocytes Causes Frequent Mosaicism for Indel Mutations

(2015) Masahiro Sato et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Rapid and highly efficient mammalian cell engineering via Cas9 protein transfection

(2015) Xiquan Liang et al.   JOURNAL OF BIOTECHNOLOGY

ASGCT and JSGT Joint Position Statement on Human Genomic Editing

(2015) Theodore Friedmann et al.   MOLECULAR THERAPY

Don’t edit the human germ line

(2015) Edward Lanphier et al.   NATURE

In vivo genome editing using Staphylococcus aureus Cas9

(2015) F. Ann Ran et al.   NATURE

Chemically modified guide RNAs enhance CRISPR-Cas genome editing in human primary cells

(2015) Ayal Hendel et al.   NATURE BIOTECHNOLOGY

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

Highly specific epigenome editing by CRISPR-Cas9 repressors for silencing of distal regulatory elements

(2015) Pratiksha I Thakore et al.   NATURE METHODS

Brave New Genome

(2015) Eric S. Lander   NEW ENGLAND JOURNAL OF MEDICINE

Generation of knock-in primary human T cells using Cas9 ribonucleoproteins

(2015) Kathrin Schumann et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

CASFISH: CRISPR/Cas9-mediated in situ labeling of genomic loci in fixed cells

(2015) Wulan Deng et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Embryo engineering study splits scientific community

(2015) J. Kaiser et al.   SCIENCE

Rationally engineered Cas9 nucleases with improved specificity

(2015) I. M. Slaymaker et al.   SCIENCE

Postnatal genome editing partially restores dystrophin expression in a mouse model of muscular dystrophy

(2015) C. Long et al.   SCIENCE

In vivo gene editing in dystrophic mouse muscle and muscle stem cells

(2015) M. Tabebordbar et al.   SCIENCE

CRISPR/Cas9 Nuclease-Mediated Gene Knock-In in Bovine-Induced Pluripotent Cells

(2015) Young Tae Heo et al.   STEM CELLS AND DEVELOPMENT

Generation of gene-target dogs using CRISPR/Cas9 system

(2015) Qingjian Zou et al.   Journal of Molecular Cell Biology

Eliminate mitochondrial diseases by gene editing in germ-line cells and embryos

(2015) Si Wang et al.   Protein & Cell

CRISPR/Cas9-mediated gene editing in human tripronuclear zygotes

(2015) Puping Liang et al.   Protein & Cell

Low-Dose Irradiation Enhances Gene Targeting in Human Pluripotent Stem Cells

(2015) Seigo Hatada et al.   Stem Cells Translational Medicine

Efficient CRISPR/Cas9-Mediated Genome Editing in Mice by Zygote Electroporation of Nuclease

(2015) Wenning Qin et al.   GENETICS

Correction of a genetic disease by CRISPR-Cas9-mediated gene editing in mouse spermatogonial stem cells

(2014) Yuxuan Wu et al.   CELL RESEARCH

Efficient Ablation of Genes in Human Hematopoietic Stem and Effector Cells using CRISPR/Cas9

(2014) Pankaj K. Mandal et al.   Cell Stem Cell

Somatic mosaicism and allele complexity induced by CRISPR/Cas9 RNA injections in mouse zygotes

(2014) Shuo-Ting Yen et al.   DEVELOPMENTAL BIOLOGY

Genetic and epigenetic stability of human spermatogonial stem cells during long-term culture

(2014) Bita Nickkholgh et al.   FERTILITY AND STERILITY

Applications of TALENs and CRISPR/Cas9 in Human Cells and Their Potentials for Gene Therapy

(2014) Jingwen Niu et al.   MOLECULAR BIOTECHNOLOGY

A molecular basis for classic blond hair color in Europeans

(2014) Catherine A Guenther et al.   NATURE GENETICS

CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos

(2014) Yanjiao Shao et al.   Nature Protocols

Multi-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells

(2014) Susan M. Byrne et al.   NUCLEIC ACIDS RESEARCH

A Modified TALEN-Based Strategy for Rapidly and Efficiently Generating Knockout Mice for Kidney Development Studies

(2014) Yunhong Liu et al.   PLoS One

International regulatory landscape and integration of corrective genome editing into in vitro fertilization

(2014) Motoko Araki et al.   Reproductive Biology and Endocrinology

Allele-specific genome editing and correction of disease-associated phenotypes in rats using the CRISPR–Cas platform

(2014) K. Yoshimi et al.   Nature Communications

Engineering human tumour-associated chromosomal translocations with the RNA-guided CRISPR–Cas9 system

(2014) R. Torres et al.   Nature Communications

Two is better than one: A case of homozygous myotonic dystrophy type 1

(2013) Jillian M. Carroll et al.   AMERICAN JOURNAL OF MEDICAL GENETICS PART A

Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression

(2013) Lei S. Qi et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Correction of a Genetic Disease in Mouse via Use of CRISPR-Cas9

(2013) Yuxuan Wu et al.   Cell Stem Cell

CAS9 transcriptional activators for target specificity screening and paired nickases for cooperative genome engineering

(2013) Prashant Mali et al.   NATURE BIOTECHNOLOGY

Programmable repression and activation of bacterial gene expression using an engineered CRISPR-Cas system

(2013) David Bikard et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Offspring from Oocytes Derived from in Vitro Primordial Germ Cell-like Cells in Mice

(2012) K. Hayashi et al.   SCIENCE

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Double-Strand Break End Resection and Repair Pathway Choice

(2011) Lorraine S. Symington et al.   Annual Review of Genetics

Reconstitution of the Mouse Germ Cell Specification Pathway in Culture by Pluripotent Stem Cells

(2011) Katsuhiko Hayashi et al.   CELL

Innovative reproductive technologies: risks and responsibilities

(2011) W. Dondorp et al.   HUMAN REPRODUCTION

In vivo genome editing restores haemostasis in a mouse model of haemophilia

(2011) Hojun Li et al.   NATURE

Epigenetic Control of Retrotransposon Expression in Human Embryonic Stem Cells

(2010) A. Macia et al.   MOLECULAR AND CELLULAR BIOLOGY

A TALE nuclease architecture for efficient genome editing

(2010) Jeffrey C Miller et al.   NATURE BIOTECHNOLOGY