CRISPR/Cas-mediated genome editing in the rat via direct injection of one-cell embryos

CasOT: a genome-wide Cas9/gRNA off-target searching tool

(2014) A. Xiao et al.   BIOINFORMATICS

Improving CRISPR-Cas nuclease specificity using truncated guide RNAs

(2014) Yanfang Fu et al.   NATURE BIOTECHNOLOGY

One-Step Generation of Mice Carrying Mutations in Multiple Genes by CRISPR/Cas-Mediated Genome Engineering

(2013) Haoyi Wang et al.   CELL

One-Step Generation of Mice Carrying Reporter and Conditional Alleles by CRISPR/Cas-Mediated Genome Engineering

(2013) Hui Yang et al.   CELL

Double Nicking by RNA-Guided CRISPR Cas9 for Enhanced Genome Editing Specificity

(2013) F. Ann Ran et al.   CELL

Generating rats with conditional alleles using CRISPR/Cas9

(2013) Yuanwu Ma et al.   CELL RESEARCH

Generation of gene-modified mice via Cas9/RNA-mediated gene targeting

(2013) Bin Shen et al.   CELL RESEARCH

Heritable gene-targeting with gRNA/Cas9 in rats

(2013) Xinli Hu et al.   CELL RESEARCH

Effect of Leukemia Inhibitory Factor and Forskolin on Establishment of Rat Embryonic Stem Cell Lines

(2013) Masumi HIRABAYASHI et al.   JOURNAL OF REPRODUCTION AND DEVELOPMENT

DNA targeting specificity of RNA-guided Cas9 nucleases

(2013) Patrick D Hsu et al.   NATURE BIOTECHNOLOGY

Targeted genome engineering in human cells with the Cas9 RNA-guided endonuclease

(2013) Seung Woo Cho et al.   NATURE BIOTECHNOLOGY

Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems

(2013) Wei Li et al.   NATURE BIOTECHNOLOGY

Knockout mice created by TALEN-mediated gene targeting

(2013) Young Hoon Sung et al.   NATURE BIOTECHNOLOGY

Efficient genome editing in zebrafish using a CRISPR-Cas system

(2013) Woong Y Hwang et al.   NATURE BIOTECHNOLOGY

High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

(2013) Vikram Pattanayak et al.   NATURE BIOTECHNOLOGY

Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

(2013) Dali Li et al.   NATURE BIOTECHNOLOGY

Whole-rat conditional gene knockout via genome editing

(2013) Andrew J Brown et al.   NATURE METHODS

Cas9 as a versatile tool for engineering biology

(2013) Prashant Mali et al.   NATURE METHODS

Generation of targeted mouse mutants by embryo microinjection of TALEN mRNA

(2013) Benedikt Wefers et al.   Nature Protocols

Genome engineering using the CRISPR-Cas9 system

(2013) F Ann Ran et al.   Nature Protocols

High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases

(2013) Zhongwei Qiu et al.   NUCLEIC ACIDS RESEARCH

Derivation of a Germline Competent Transgenic Fischer 344 Embryonic Stem Cell Line

(2013) Hongsheng Men et al.   PLoS One

Heritable and Precise Zebrafish Genome Editing Using a CRISPR-Cas System

(2013) Woong Y. Hwang et al.   PLoS One

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Generation of mutant mice by pronuclear injection of circular plasmid expressing Cas9 and single guided RNA

(2013) Daisuke Mashiko et al.   Scientific Reports

Transposon-mediated transgenesis, transgenic rescue, and tissue-specific gene expression in rodents and rabbits

(2012) Katharina Katter et al.   FASEB JOURNAL

Generation of Rag1-knockout immunodeficient rats and mice using engineered meganucleases

(2012) Séverine Ménoret et al.   FASEB JOURNAL

Precision genome engineering with programmable DNA-nicking enzymes

(2012) E. Kim et al.   GENOME RESEARCH

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

Germline Transmission of a Novel Rat Embryonic Stem Cell Line Derived from Transgenic Rats

(2012) Hongsheng Men et al.   STEM CELLS AND DEVELOPMENT

Knockout rats generated by embryo microinjection of TALENs

(2011) Laurent Tesson et al.   NATURE BIOTECHNOLOGY

High-frequency genome editing using ssDNA oligonucleotides with zinc-finger nucleases

(2011) Fuqiang Chen et al.   NATURE METHODS

Generating gene knockout rats by homologous recombination in embryonic stem cells

(2011) Chang Tong et al.   Nature Protocols

Evolution and classification of the CRISPR–Cas systems

(2011) Kira S. Makarova et al.   NATURE REVIEWS MICROBIOLOGY

The Mechanism of Double-Strand DNA Break Repair by the Nonhomologous DNA End-Joining Pathway

(2010) Michael R. Lieber   Annual Review of Biochemistry

Overlap extension PCR cloning: a simple and reliable way to create recombinant plasmids

(2010) Anton Bryksin et al.   BIOTECHNIQUES

Rat transgenesis via embryonic stem cells electroporated with the Kusabira-orange gene

(2010) Masumi Hirabayashi et al.   MOLECULAR REPRODUCTION AND DEVELOPMENT

Production of p53 gene knockout rats by homologous recombination in embryonic stem cells

(2010) Chang Tong et al.   NATURE

The CRISPR/Cas bacterial immune system cleaves bacteriophage and plasmid DNA

(2010) Josiane E. Garneau et al.   NATURE

Self versus non-self discrimination during CRISPR RNA-directed immunity

(2010) Luciano A. Marraffini et al.   NATURE

Targeted integration in rat and mouse embryos with zinc-finger nucleases

(2010) Xiaoxia Cui et al.   NATURE BIOTECHNOLOGY

Generation of Knockout Rats with X-Linked Severe Combined Immunodeficiency (X-SCID) Using Zinc-Finger Nucleases

(2010) Tomoji Mashimo et al.   PLoS One

Gene targeting in the rat: advances and opportunities

(2010) Howard J. Jacob et al.   TRENDS IN GENETICS

Breaking the Code of DNA Binding Specificity of TAL-Type III Effectors

(2009) J. Boch et al.   SCIENCE

A Simple Cipher Governs DNA Recognition by TAL Effectors

(2009) M. J. Moscou et al.   SCIENCE

Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases

(2009) Aron M. Geurts et al.   SCIENCE

A caveat in mouse genetic engineering: ectopic gene targeting in ES cells by bidirectional extension of the homology arms of a gene replacement vector carrying human PARP-1

(2008) Aswin Mangerich et al.   TRANSGENIC RESEARCH