State-of-the-Art Native Mass Spectrometry and Ion Mobility Methods to Monitor Homogeneous Site-Specific Antibody-Drug Conjugates Synthesis

Middle level IM-MS and CIU experiments for improved therapeutic immunoglobulin subclass fingerprinting

(2020) Thomas Botzanowski et al.   ANALYTICAL CHEMISTRY

Antibody–Drug Conjugates: The Last Decade

(2020) Nicolas Joubert et al.   Pharmaceuticals

Cutting-edge multi-level analytical and structural characterization of antibody-drug conjugates: present and future

(2019) Alain Beck et al.   Expert Review of Proteomics

CIUSuite 2: Next-Generation Software for the Analysis of Gas-Phase Protein Unfolding Data

(2019) Daniel A. Polasky et al.   ANALYTICAL CHEMISTRY

An Industry Perspective on Forced Degradation Studies of Biopharmaceuticals: Survey Outcome and Recommendations

(2019) Jennifer Halley et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Native size-exclusion chromatography-mass spectrometry: suitability for antibody–drug conjugate drug-to-antibody ratio quantitation across a range of chemotypes and drug-loading levels

(2019) Jay Jones et al.   mAbs

Collision induced unfolding of isolated proteins in the gas phase: past, present, and future

(2018) Sugyan M Dixit et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Hyphenation of size exclusion chromatography to native ion mobility mass spectrometry for the analytical characterization of therapeutic antibodies and related products

(2018) Anthony Ehkirch et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Stability assessment of antibody-drug conjugate Trastuzumab emtansine in comparison to parent monoclonal antibody using orthogonal testing protocol

(2018) Hoda E. Mohamed et al.   JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

Characterization of in vivo biotransformations for trastuzumab emtansine by high-resolution accurate-mass mass spectrometry

(2018) Jintang He et al.   mAbs

Practical approaches for overcoming challenges in heightened characterization of antibody-drug conjugates with new methodologies and ultrahigh-resolution mass spectrometry

(2018) Olga V. Friese et al.   mAbs

Is hydrophobic interaction chromatography the most suitable technique to characterize site-specific antibody-drug conjugates?

(2018) Valentina D’Atri et al.   JOURNAL OF CHROMATOGRAPHY A

Quantitative collision-induced unfolding differentiates model antibody-drug conjugates

(2018) Yuwei Tian et al.   PROTEIN SCIENCE

Forced degradation of recombinant monoclonal antibodies: A practical guide

(2017) Christine Nowak et al.   mAbs

Insights from native mass spectrometry approaches for top- and middle- level characterization of site-specific antibody-drug conjugates

(2017) Thomas Botzanowski et al.   mAbs

Strategies and challenges for the next generation of antibody–drug conjugates

(2017) Alain Beck et al.   NATURE REVIEWS DRUG DISCOVERY

Cutting-edge mass spectrometry methods for the multi-level structural characterization of antibody-drug conjugates

(2016) Alain Beck et al.   Expert Review of Proteomics

Antibody-drug conjugate characterization by chromatographic and electrophoretic techniques

(2016) Tao Chen et al.   JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES

Physical and Chemical Stability of Antibody Drug Conjugates: Current Status

(2016) Philip L. Ross et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Effects of antibody, drug and linker on the preclinical and clinical toxicities of antibody-drug conjugates

(2016) Heather Donaghy   mAbs

Collision Induced Unfolding of Intact Antibodies: Rapid Characterization of Disulfide Bonding Patterns, Glycosylation, and Structures

(2015) Yuwei Tian et al.   ANALYTICAL CHEMISTRY

Chemoenzymatic Conjugation of Toxic Payloads to the Globally Conserved N-Glycan of Native mAbs Provides Homogeneous and Highly Efficacious Antibody–Drug Conjugates

(2015) Remon van Geel et al.   BIOCONJUGATE CHEMISTRY

MAPN: First-in-Class Reagent for Kinetically Resolved Thiol-to-Thiol Conjugation

(2015) Oleksandr Koniev et al.   BIOCONJUGATE CHEMISTRY

CBTF: New Amine-to-Thiol Coupling Reagent for Preparation of Antibody Conjugates with Increased Plasma Stability

(2015) Sergii Kolodych et al.   BIOCONJUGATE CHEMISTRY

Glycans of Antibodies as a Specific Site for Drug Conjugation Using Glycosyltransferases

(2015) Pradman K. Qasba   BIOCONJUGATE CHEMISTRY

Rapid characterization of biotherapeutic proteins by size-exclusion chromatography coupled to native mass spectrometry

(2015) Markus Haberger et al.   mAbs

Reducing hydrophobicity of homogeneous antibody-drug conjugates improves pharmacokinetics and therapeutic index

(2015) Robert P Lyon et al.   NATURE BIOTECHNOLOGY

Native mass spectrometry and ion mobility characterization of trastuzumab emtansine, a lysine-linked antibody drug conjugate

(2015) Julien Marcoux et al.   PROTEIN SCIENCE

Innovative Native MS Methodologies for Antibody Drug Conjugate Characterization: High Resolution Native MS and IM-MS for Average DAR and DAR Distribution Assessment

(2014) François Debaene et al.   ANALYTICAL CHEMISTRY

Measurement of in Vivo Drug Load Distribution of Cysteine-Linked Antibody–Drug Conjugates Using Microscale Liquid Chromatography Mass Spectrometry

(2014) Shawna Mae Hengel et al.   ANALYTICAL CHEMISTRY

Transglutaminase-Based Chemo-Enzymatic Conjugation Approach Yields Homogeneous Antibody–Drug Conjugates

(2014) Patrick Dennler et al.   BIOCONJUGATE CHEMISTRY

Chemoenzymatic Fc Glycosylation via Engineered Aldehyde Tags

(2014) Elizabeth L. Smith et al.   BIOCONJUGATE CHEMISTRY

High-Throughput Biophysical Analysis of Protein Therapeutics to Examine Interrelationships Between Aggregate Formation and Conformational Stability

(2013) Rajoshi Chaudhuri et al.   AAPS Journal

Development of a Native Nanoelectrospray Mass Spectrometry Method for Determination of the Drug-to-Antibody Ratio of Antibody–Drug Conjugates

(2013) Jia Chen et al.   ANALYTICAL CHEMISTRY

Investigation into Temperature-Induced Aggregation of an Antibody Drug Conjugate

(2013) Nia S. Beckley et al.   BIOCONJUGATE CHEMISTRY

Mass Spectrometric Characterization of Transglutaminase Based Site-Specific Antibody–Drug Conjugates

(2013) Santiago E. Farias et al.   BIOCONJUGATE CHEMISTRY

SGN-CD33A: a novel CD33-targeting antibody-drug conjugate using a pyrrolobenzodiazepine dimer is active in models of drug-resistant AML

(2013) M. S. Kung Sutherland et al.   BLOOD

Location Matters: Site of Conjugation Modulates Stability and Pharmacokinetics of Antibody Drug Conjugates

(2013) Pavel Strop et al.   CHEMISTRY & BIOLOGY

Site-specific antibody drug conjugates for cancer therapy

(2013) Siler Panowski et al.   mAbs

Native Intact Mass Determination of Antibodies Conjugated with Monomethyl Auristatin E and F at Interchain Cysteine Residues

(2012) John F. Valliere-Douglass et al.   ANALYTICAL CHEMISTRY

Synthesis of site-specific antibody-drug conjugates using unnatural amino acids

(2012) J. Y. Axup et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Breaking the Light and Heavy Chain Linkage of Human Immunoglobulin G1 (IgG1) by Radical Reactions

(2011) Boxu Yan et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Fragmentation of monoclonal antibodies

(2011) Josef Vlasak et al.   mAbs

Collision Cross Sections of Proteins and Their Complexes: A Calibration Framework and Database for Gas-Phase Structural Biology

(2010) Matthew F. Bush et al.   ANALYTICAL CHEMISTRY

Physicochemical Stability of the Antibody−Drug Conjugate Trastuzumab-DM1: Changes due to Modification and Conjugation Processes

(2010) Aditya A. Wakankar et al.   BIOCONJUGATE CHEMISTRY

Histidine Residue Mediates Radical-induced Hinge Cleavage of Human IgG1

(2010) Zac Yates et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Site-specific conjugation of a cytotoxic drug to an antibody improves the therapeutic index

(2008) Jagath R Junutula et al.   NATURE BIOTECHNOLOGY

Ion mobility–mass spectrometry analysis of large protein complexes

(2008) Brandon T Ruotolo et al.   Nature Protocols