Article
Oncology
Veronica L. Nagle, Charli Ann J. Hertz, Kelly E. Henry, Maya S. Graham, Carl Campos, Nagavarakishore Pillarsetty, Andrea Schietinger, Ingo K. Mellinghoff, Jason S. Lewis
Summary: This study explores the use of anti-human-CD4 minibody for antibody-based PET to visualize human CD4(+) T cells. Through in vitro and in vivo experiments, it is found that this method can accurately detect CD4(+) T cells without impacting their abundance, proliferation, and activation state. In humanized mice, this method can also visualize the distribution of CD4(+) T cells in peripheral tissues and brain tumors.
MOLECULAR CANCER THERAPEUTICS
(2022)
Review
Cell Biology
Sofya A. Erokhina, Maria A. Streltsova, Leonid M. Kanevskiy, Maria V. Grechihina, Alexander M. Sapozhnikov, Elena I. Kovalenko
Summary: HLA-DR+ NK cells display characteristics of both NK cells and dendritic cells, and are considered significant players in the innate immune system due to their functional activities in producing cytokines, degranulation, and antigen presentation to T cells.
JOURNAL OF LEUKOCYTE BIOLOGY
(2021)
Review
Immunology
Karolina D. Witt
Summary: MHC class I antigen processing is a less recognized area in nonviral host-pathogen interactions, involving immunology and cell biology. This review focuses on the MHC-I antigen processing pathway and alternative sources of antigens, particularly in the context of Mycobacterium tuberculosis (Mtb) as an intracellular pathogen. It explores how Mtb manipulates host immunity for survival and proposes directions for MHC-I-focused approaches in developing vaccines against tuberculosis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Cell Biology
Leila Fonderflick, Timothee Baudu, Olivier Adotevi, Michael Guittaut, Pascale Adami, Regis Delage-Mourroux
Summary: Vaccine therapy is a promising method of research to promote T cell immune response and to develop novel antitumor immunotherapy protocols. Increasing evidence shows that autophagy plays a role in antigen processing and presentation to T cells.
Article
Immunology
Paula Ruibal, Ian Derksen, Marjolein van Wolfswinkel, Linda Voogd, Kees L. M. C. Franken, Angela F. El Hebieshy, Thorbald van Hall, Tom A. W. Schoufour, Ruud H. Wijdeven, Tom H. M. Ottenhoff, Ferenc A. Scheeren, Simone A. Joosten
Summary: There is growing interest in HLA-E-restricted T-cell responses as a potential vaccination target. This study developed a method for efficient peptide thermal exchange on HLA-E monomers and multimers, allowing high-throughput production. The optimized conditions for detection and analysis of peptide-specific T cells revealed new insights into the interaction between HLA-E and NKG2A/CD94 receptors.
Review
Biochemistry & Molecular Biology
M. L. M. Jongsma, J. Neefjes, R. M. Spaapen
Summary: MHC-I molecules present a blueprint of the intracellular proteome to T cells for immune response, but pathogens and tumor cells can downmodulate MHC-I mediated antigen presentation to evade immune surveillance. While the fundamental rules of antigen presentation are well understood, new modules of regulation in this system continue to be uncovered.
MOLECULAR IMMUNOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yoseph Asmelash Gebru, Haripriya Gupta, Hyeong Seop Kim, Jung A. Eom, Goo Hyun Kwon, Eunju Park, Jin-Ju Jeong, Sung-Min Won, Satya Priya Sharma, Raja Ganesan, Dong Joon Kim, Ki Tae Suk
Summary: NAFLD is characterized by hepatic accumulation of excess lipids, and various T cell subsets and NK cells have been implicated in its progression, with different functions and phenotypes usually leading to proinflammatory effects. The gut microbiota can influence intrahepatic T cell subsets, and NK cells can accumulate in the liver in response to pathogens and tumors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Xiongfei Xu, Haihui Gu, Hongwei Li, Suizhi Gao, Xiaohan Shi, Jing Shen, Bo Li, Huan Wang, Kailian Zheng, Zhuo Shao, Peng Cheng, Zhanshan Cha, Siying Peng, Yongzhan Nie, Zhaoshen Li, Shiwei Guo, Baohua Qian, Gang Jin
Summary: This article presents a feasible large-cohort humanized mouse model for evaluating the efficacy and safety of various cancer immunotherapy approaches. Additionally, this model can mimic the clinical actual setting and aid in the development of more effective combination therapies.
Article
Immunology
Rui Zheng, Yuankun Chen, Yiting Zhang, Sixin Liang, Xiaojuan Zhao, Yiyi Wang, Pengju Wang, Ruotong Meng, Angang Yang, Bo Yan
Summary: Our study explores the effect of low-affinity CARs using humanized scFvs on the function of CAR-T cells. We find that moderately reducing the affinity of CARs can maintain anti-tumor efficacy and improve the safety of CAR therapy both in vitro and in vivo. In addition, T cells expressing the VL domain only antibody show long-lasting tumor elimination capability and lower cytokine levels.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Vita Golubovskaya, Hua Zhou, Feng Li, Michael Valentine, Jinying Sun, Robert Berahovich, Shirley Xu, Milton Quintanilla, Man Cheong Ma, John Sienkiewicz, Yanwei Huang, Lijun Wu
Summary: CAR-T cell therapy, specifically targeting CD19, has been approved by the FDA for leukemia and lymphoma treatment. This study introduces novel CD37 and CD37-CD19 CAR-T cells, demonstrating their specific targeting abilities and laying the foundation for future clinical studies.
Article
Immunology
Jimena Alvarez Freile, Yuzhu Qi, Lisa Jacob, Maria Franceskin Lobo, Harm Jan Lourens, Gerwin Huls, Edwin Bremer
Summary: Investigations into the strength of antigen-specific responses in vitro are crucial for early-phase research of immunotherapeutic approaches. This study presents a rapid luminescence-based method using HPV16 E7(11-20) peptide as a model antigen to evaluate MHC-dependent antigen-specific T cell responses in vitro. The method has important implications for assessing the impact of tumor microenvironment and immune checkpoint inhibitors, as well as accelerating the development of novel immunotherapies.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Muhammad Zaeem Noman, Irene Adelaide Bocci, Manale Karam, Kris Van Moer, Manon Bosseler, Akinchan Kumar, Guy Berchem, Christian Auclair, Bassam Janji
Summary: The study reveals that Harmine, by increasing the expression of MHC-I, enhances the immune features of melanoma cells and inhibits tumor growth. Combination therapy with ACB1801 and anti-PD1 shows improved therapeutic benefits. Clinical data further supports the use of ACB1801 in combination with anti-PD1/PD-L1 therapy to improve survival in cancer patients with a defect in MHC-I expression.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
William H. Palmer, Laura Ann Leaton, Ana Campos Codo, Bergren Crute, James Roest, Shiying Zhu, Jan Petersen, Richard P. Tobin, Patrick S. Hume, Matthew Stone, Adrie van Bokhoven, Mark E. Gerich, Martin D. McCarter, Yuwen Zhu, William J. Janssen, Julian P. Vivian, John Trowsdale, Andrew Getahun, Jamie Rossjohn, John Cambier, Liyen Loh, Paul J. Norman
Summary: Most human killer cell immunoglobulin-like receptors (KIR) are expressed by natural killer (NK) cells and recognize HLA class I molecules as ligands. However, KIR3DL3 is expressed in γδ and CD8(+) T cells and recognizes the B7 family ligand HHLA2, playing a role in immune checkpoint targeting. KIR3DL3(+) T cells in peripheral blood have an activated transitional memory phenotype and are hypofunctional, with biased T cell receptor (TCR) usage. Additionally, KIR3DL3 expression can be influenced by polymorphisms and variants in the proximal promoter and residue 86. These findings have implications for personalized targeting of KIR3DL3/HHLA2 checkpoint inhibition.
SCIENCE IMMUNOLOGY
(2023)
Article
Virology
Leon C. W. Lin, Sarah N. Croft, Nathan P. Croft, Yik Chun Wong, Stewart A. Smith, Swee-Seong Tang, Anthony W. Purcell, David C. Tscharke
Summary: The study investigated the CD8(+) T cell priming pathways for Vaccinia virus (VACV) by engineering the virus to express inhibitors of antigen presentation. While the inhibitors had diverse impacts on CD8(+) T cell responses, unexpectedly, the direct priming of CD8(+) T cells by poxviruses was robust enough to withstand potent viral inhibitors of antigen presentation. This demonstrates the flexibility and robustness of immune processes in activating immune responses required to fight infection.
JOURNAL OF VIROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Takataro Fukuhara, Yoshihiro Ueda, Sung-Il Lee, Tokifumi Odaka, Shinsuke Nakajima, Jun-Ichi Fujisawa, Kazu Okuma, Makoto Naganuma, Kazuichi Okazaki, Naoyuki Kondo, Yuji Kamioka, Mitsuru Matsumoto, Tatsuo Kinashi
Summary: In humanized mice, immunization leads to an increase in HLA-DR+ cells, including CD205(+) dendritic cells, which promotes HLA-restricted thymocyte expansion. This finding offers a unique opportunity to generate humanized mice with ease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
