Article
Biology
Sriram Srikant, Chantal K. Guegler, Michael T. Laub
Summary: This study reveals the mechanism by which phages overcome bacterial defense systems through experimental evolution. The emergence of one counter-defense mechanism can lead to the loss of other mechanisms, thus constraining the host range of phages.
Article
Multidisciplinary Sciences
Junxia Wang, Liang Li, Yingkun Zhang, Kaifeng Zhao, Xiaofeng Chen, Haicong Shen, Yuanqiang Chen, Jia Song, Yuqiang Ma, Chaoyong Yang, Hongming Ding, Zhi Zhu
Summary: Efficient molecular selection is crucial for various biomedical applications. By utilizing deterministic lateral-displacement microfluidics, the equilibrium of dissociation can be greatly shifted, leading to improved selection efficiency and better discrimination of ligand affinity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Rebecca K. Lau, Eray Enustun, Yajie Gu, Justin Nguyen, Kevin D. Corbett
Summary: Bacteria have evolved diverse immune systems to protect themselves from phage infection. Researchers have discovered a transcriptional regulator module associated with hundreds of CBASS immune systems and demonstrated its role in driving the expression of the CBASS system in response to DNA damage. This finding highlights a mechanism by which bacterial immune systems can sense and respond to cellular stress.
Article
Chemistry, Physical
Joel D. Mallory, Xian F. Mallory, Anatoly B. Kolomeisky, Oleg A. Igoshin
Summary: The study reveals that proofreading optimizes the rate of virus production, but further optimization would lead to a significant increase in the cost. Minimizing cost is crucial for functional virus production.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Review
Virology
Tea Glonti, Jean-Paul Pirnay
Summary: This review summarizes the validated methods for phage selection, host range expansion, and lytic activity determination in phage therapy, highlighting their advantages and disadvantages. It suggests that combining in vitro quantitative and qualitative measurements with in vivo experiments could predict the potential outcomes of clinical applications.
Article
Microbiology
Larissa Kever, Ael Hardy, Tom Luthe, Max Huennefeld, Cornelia Gaetgens, Lars Milke, Johanna Wiechert, Johannes Wittmann, Cristina Moraru, Jan Marienhagen, Julia Frunzke
Summary: Predation by phages is a major driver of bacterial evolution. This study reveals that aminoglycosides, a type of antibiotics produced by Streptomyces, have potent antiviral properties and can block an early step of phage infection. The supernatants from natural aminoglycoside producers also show broad inhibitory effects on phage propagation. Acetylation of the aminoglycoside antibiotic apramycin abolishes its antibacterial effect but retains its antiviral properties. These findings expand our understanding of aminoglycoside functions and suggest that they may provide protection against phage predation at the community level.
Article
Biochemistry & Molecular Biology
Jilin Liu, Qian Li, Xiaojie Wang, Zhenzhen Liu, Qing Ye, Tao Liu, Saifu Pan, Nan Peng
Summary: In this study, a viral-encoded anti-CRISPR protein, AcrIIIB2, was identified to inhibit Type III-B CRISPR-Cas immunity. The interaction between AcrIIIB2 and Cmr4 alpha subunit and the mechanism by which AcrIIIB2 inhibits the activity of Cmr-alpha ribonucleoprotein complex were elucidated.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Kevin J. Forsberg
Summary: CRISPR-Cas immune systems protect bacteria and archaea from viral infection, prompting viruses to develop anti-CRISPRs (Acrs), inhibitors of these systems. The genomes of bacteria, archaea, and their viruses encode diverse examples of immune and counter-immune proteins. This review discusses methods used to identify Acrs and speculates on the future of Acr discovery, emphasizing their importance in host-virus conflicts and biotechnology applications.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Plant Sciences
Feifei Liu, Timothy J. O'Donnell, Eun-Jung Park, Sasha Kovacs, Kenzo Nakamura, Asim Dave, Yuheng Luo, Rui Sun, Marisa Wall, Supakit Wongwiwatthananukit, Dane Kaohelani Silva, Philip G. Williams, John M. Pezzuto, Leng Chee Chang
Summary: Sixteen new quinoline alkaloids and ten known analogs were isolated from the roots of Waltheria indica and their structures were determined through various methods. The anti-inflammatory potential of these compounds was evaluated using cell culture models and it was found that some compounds showed significant inhibition of inflammation, supporting the traditional use of Waltheria indica for treating inflammatory-related disorders.
JOURNAL OF NATURAL PRODUCTS
(2023)
Article
Environmental Sciences
Shanchun Yan, Mingtao Tan, Lin Zheng, Hongfei Wu, Kai Wang, Rusong Chai, Dun Jiang
Summary: Heavy metal pollution is a serious global environmental problem that affects plant insect resistance and pest occurrence. This study investigated the defense response of Fraxinus mandshurica seedlings to Hyphantria cunea larvae under Cd stress, focusing on chemical, physical, and elemental defense. The results showed that Cd-treated seedlings attracted H. cunea larvae but reduced their body weight and survival rate. The increase in attractant metabolites and the decrease in repellent metabolites in leaf volatiles were responsible for the high attractant activity to H. cunea larvae under Cd treatment. The study also identified a reduction in physical defense, chemical defense, and their combination in Cd-treated seedlings through TOPSIS analysis.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Review
Microbiology
Julia E. Egido, Ana Rita Costa, Cristian Aparicio-Maldonado, Pieter-Jan Haas, Stan J. J. Brouns
Summary: This article explores the defense mechanisms of bacteriophages and their relevance for the potential clinical use of bacteriophages as therapeutic agents, suggesting new directions for research.
FEMS MICROBIOLOGY REVIEWS
(2022)
Article
Virology
Cedric Woudstra, Sophie A. Granier
Summary: This study investigates the anti-phage defenses in Salmonella Typhimurium using bioinformatics and identifies nucleic acid degradation and abortive infection mechanisms. Mobile genetic elements containing anti-phage systems were also found in Salmonella and the Enterobacteriaceae family. This research provides insights for designing preventive measures against the spread of phage resistance.
Editorial Material
Microbiology
Mark R. Sullivan, Eric J. Rubin, Charles L. Dulberger
Summary: Mycobacterium abscessus (Mab) is highly tolerant to current antibiotic therapies, leading to a high failure rate of standard care. Phages may offer a promising alternative treatment with few side effects. Two studies shed light on the natural phage complement of Mab and the factors that may drive susceptibility to these phages, paving the way for more effective phage therapy development.
Article
Physics, Multidisciplinary
Chenghong Wang, Kun Wang, Zhu-Jun Zheng
Summary: This paper presents a new method for constructing maximally entangled states and provides examples in specific scenarios. It is of great importance in the field of related research.
Review
Microbiology
Konstantina Akritidou, Bryan H. Thurtle-Schmidt
Summary: Bacteriophages are a threat to bacteria, and bacteria have evolved various defense systems. While some of these systems are poorly understood, recent studies have revealed the function of OLD nucleases in multiple defense systems.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Aaron T. Whiteley, Alex J. Pollock, Daniel A. Portnoy
CELL HOST & MICROBE
(2015)
Article
Chemistry, Multidisciplinary
Colleen A. Kellenberger, Chen Chen, Aaron T. Whiteley, Daniel A. Portnoy, Ming C. Hammond
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2015)
Article
Microbiology
Michelle L. Reniere, Aaron T. Whiteley, Daniel A. Portnoy
Article
Biochemistry & Molecular Biology
Aaron T. Whiteley, Nicholas E. Garelis, Bret N. Peterson, Philip H. Choi, Liang Tong, Joshua J. Woodward, Daniel A. Portnoy
MOLECULAR MICROBIOLOGY
(2017)
Article
Multidisciplinary Sciences
Gabriel Mitchell, Mandy I. Cheng, Chen Chen, Brittney N. Nguyen, Aaron T. Whiteley, Sara Kianian, Jeffery S. Cox, Douglas R. Green, Kent L. McDonald, Daniel A. Portnoy
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Article
Microbiology
Jonathan L. Portman, Samuel B. Dubensky, Bret N. Peterson, Aaron T. Whiteley, Daniel A. Portnoy
Article
Biochemistry & Molecular Biology
Wen Zhou, Aaron T. Whiteley, Carina C. de Oliveira Mann, Benjamin R. Morehouse, Radosaw P. Nowak, Eric S. Fischer, Nathanael S. Gray, John J. Mekalanos, Philip J. Kranzusch
Article
Multidisciplinary Sciences
Aaron T. Whiteley, James B. Eaglesham, Carina C. de Oliveira Mann, Benjamin R. Morehouse, Brianna Lowey, Eric A. Nieminen, Olga Danilchanka, David S. King, Amy S. Y. Lee, John J. Mekalanos, Philip J. Kranzusch
Article
Biochemistry & Molecular Biology
Rebecca K. Lau, Qiaozhen Ye, Erica A. Birkholz, Kyle R. Berg, Lucas Patel, Ian T. Mathews, Jeramie D. Watrous, Kaori Ego, Aaron T. Whiteley, Brianna Lowey, John J. Mekalanos, Philip J. Kranzusch, Mohit Jain, Joe Pogliano, Kevin D. Corbett
Article
Biochemistry & Molecular Biology
Brianna Lowey, Aaron T. Whiteley, Alexander F. A. Keszei, Benjamin R. Morehouse, Ian T. Mathews, Sadie P. Antine, Victor J. Cabrera, Dmitry Kashin, Percy Niemann, Mohit Jain, Frank Schwede, John J. Mekalanos, Sichen Shao, Amy S. Y. Lee, Philip J. Kranzusch
Article
Microbiology
Bret N. Peterson, Megan K. M. Young, Shukun Luo, Jeffrey Wang, Aaron T. Whiteley, Joshua J. Woodward, Liang Tong, Jue D. Wang, Daniel A. Portnoy
Editorial Material
Microbiology
Emily M. Kibby, Aaron T. Whiteley
JOURNAL OF BACTERIOLOGY
(2020)
Article
Multidisciplinary Sciences
Denis Chang, Aaron T. Whiteley, Katlynn Bugda Gwilt, Wayne Lencer, John J. Mekalanos, Jay R. Thiagarajah
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Immunology
Aaron T. Whiteley, Brittany R. Ruhland, Mauna B. Edrozo, Michelle L. Reniere
INFECTION AND IMMUNITY
(2017)