Review
Nutrition & Dietetics
David E. Williams
Summary: Studies have shown the effectiveness of I3C and DIM in cancer prevention, especially in pre-clinical models, and they have shown promise in clinical trials. The focus of research has been on understanding the mechanisms of action of DIM in preventing breast and prostate cancer, as well as comparing the impact of dietary intake of cruciferous vegetables containing I3C/DIM versus supplementation.
FRONTIERS IN NUTRITION
(2021)
Review
Oncology
Octavio Daniel Reyes-Hernandez, Gabriela Figueroa-Gonzalez, Laura Itzel Quintas-Granados, Stephany Celeste Gutierrez-Ruiz, Hector Hernandez-Parra, Alejandra Romero-Montero, Maria Luisa Del Prado-Audelo, Sergio Alberto Bernal-Chavez, Hernan Cortes, Sheila I. Pena-Corona, Lashyn Kiyekbayeva, Dilek Arslan Atessahin, Tamar Goloshvili, Gerardo Leyva-Gomez, Javad Sharifi-Rad
Summary: The dietary compounds I3C and DIM found in cruciferous vegetables have shown potential in cancer prevention. Clinical and preclinical trials have demonstrated their antioxidant, cancer preventive, and anti-tumor activities. However, the precise mechanism by which they exert these effects is still not fully understood.
CANCER CELL INTERNATIONAL
(2023)
Article
Pharmacology & Pharmacy
Jessica Brandao Reolon, Camila Parcianello Saccol, Barbara Felin Osmari, Daiane Britto de Oliveira, Vinicius Costa Prado, Fernanda Licker Cabral, Lucas Saldanha da Rosa, Giancarlo Cervo Rechia, Daniela Bitencourt Rosa Leal, Leticia Cruz
Summary: This study aimed to develop a bilayer film for topical melanoma therapy by incorporating nanocapsules containing 3,3 '-diindolylmethane (DIM) with antitumor activity. The nanofilms showed appropriate behavior for cutaneous application and controlled DIM release profile, and increased the permeation of DIM into the epidermis. The combination of karaya and gellan gum in the bilayer films demonstrated potential in the topical treatment of melanoma.
Article
Microbiology
Yong-Guy Kim, Jin-Hyung Lee, Sunyoung Park, Jintae Lee
Summary: The study investigated the antibacterial and antibiofilm activities of 20 indoles against Cutibacterium acnes, a bacterium that inhabits human skin and is implicated in acne vulgaris formation. The results showed that indole-3-carbinol and 3,3'-diindolylmethane significantly inhibited biofilm formation by C. acnes. Furthermore, 3,3'-diindolylmethane successfully inhibited multispecies biofilm formation by C. acnes, Staphylococcus aureus, and Candida albicans. Transcriptional analyses showed that 3,3'-diindolylmethane inhibited the expressions of several biofilm-related genes in C. acnes and inhibited hyphal formation and cell aggregation by C. albicans. These findings suggest that 3,3'-diindolylmethane could be a potential treatment for acne vulgaris and biofilm-associated infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Applied
Jia Chen Qian, Dan Liu, Li Ping Lin, Wen Jing Zhu, Ren Xiang Tan
Summary: This study demonstrates the presence of a family of low-abundance high-order indole oligomers in kimchi, derived from cruciferous vegetables. One of the indole tetramers exhibits significant toxicity towards tumor cells. The findings provide valuable information about kimchi and may inspire the development of indole-based molecules.
Article
Pharmacology & Pharmacy
Kakarla Ramakrishna, Neha Singh, Sairam Krishnamurthy
Summary: Diindolylmethane (DIM) is a metabolite of indole-3-carbinol (I3C) that plays a crucial role in inhibiting platelet aggregation and thrombus generation. In silico studies suggest that DIM modulates platelet interaction with glycoproteinVI (GPVI) and purinergic receptor Y-12 (P2Y12) receptors more effectively than I3C. In vitro and in vivo experiments demonstrate that DIM significantly inhibits platelet aggregation, reduces reactive oxygen species (ROS) and thromboxane 2 (TXB2), while increasing cyclic adenosine monophosphate (cAMP) levels and extending time to occlusion (TTO). DIM acts as an antiplatelet aggregation and antithrombotic agent, making it a potential treatment for thrombotic diseases.
EUROPEAN JOURNAL OF PHARMACOLOGY
(2022)
Article
Plant Sciences
Alin Finkelshtein, Hala Khamesa, Luu Anh Tuan, Manely Rabanim, Daniel A. Chamovitz
Summary: The hydrolysis product of indole-3-methylglucosinolate, indole-3-carbinol (I3C), is toxic to herbivorous insects and pathogens, while extensively studied in mammals for its properties in cancer prevention and treatment. A novel Arabidopsis mutant, ICT1, has been identified to be tolerant to I3C, with the tolerance mechanism involving the S30 ribosomal protein. The tolerance is specific to I3C and maintained the antagonistic action of I3C on auxin signaling through an auxin-independent mechanism.
Article
Biochemical Research Methods
Alexander P. Lakeev, Elena A. Yanovskaya, Daria V. Tsuran, Olga S. Bryushinina, Natalia Yu. Abdrashitova, Yulia G. Zyuz'kova, Vladimir V. Udut, Vsevolod I. Kiselev, Igor N. Kuznetsov
Summary: This study established an HPLC-MS/MS method for quantifying DIM in human plasma. The method showed good sensitivity, accuracy, and precision, allowing for DIM quantification in the concentration range of 5-500 ng/ml. 4-Methoxy-1-methylindole was used as an internal standard during sample extraction and analysis.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Pharmacology & Pharmacy
Kakarla Ramakrishna, Sangeetha Sinku, Shreyasi Majumdar, Neha Singh, T. A. Gajendra, Asha Rani, Sairam Krishnamurthy
Summary: This study investigated the hepatic and neuroprotective roles of Indole-3-carbinol (I3C) in thioacetamide (TAA)-induced hepatic encephalopathy. The results showed that I3C could significantly decrease the levels of ammonia in the blood, liver, and brain, improve liver function, inhibit CYP2E1 enzyme activity in the liver and brain, alleviate mitochondrial dysfunction, oxidative stress, inflammation, and apoptosis, and improve the histological abnormalities in the brain.
Review
Food Science & Technology
Darshika Amarakoon, Wu-Joo Lee, Gillian Tamia, Seong-Ho Lee
Summary: Indole-3-carbinol (I3C) and 3,3'-diindolylmethane (DIM), two compounds found in cruciferous vegetables, have shown potential in preventing and treating various chronic diseases.
ANNUAL REVIEW OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Qi Li, Boyu Xia, Jingjing Wu, Xiaomei Yuan, Xu Lu, Chao Huang, Hongcheng Gu, Koulong Zheng, Qingsheng You, Kun Liu
Summary: The study demonstrates that administration of indole-3-carbinol (I3C) protects the heart from myocardial ischemia/reperfusion injury (MIRI) through its anti-apoptotic, antioxidant, and anti-inflammatory effects.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Shengying Pan, Yaoying Ma, Rongrong Yang, Xu Lu, Qingsheng You, Ting Ye, Chao Huang
Summary: The study demonstrates that the I3C treatment can prevent chronic stress-induced behavioral abnormalities, particularly depression-like behaviors, in mice. This treatment selectively inhibits neuroinflammation and oxido-nitrosative stress caused by chronic stress.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Plant Sciences
Tao Wang, Derui Zhang, Boming Yang, Nana Su, Jin Cui
Summary: This study found that blue light treatment increased the accumulation of indole-3-carbinol (I3C) in plants, while exogenous salicylic acid (SA) treatment significantly inhibited the accumulation of I3C. Gene expression analysis showed that blue light promoted differential expression of genes related to plant hormone signal transduction pathways. The results suggest that SA negatively regulates blue light-induced I3C accumulation.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Georgia Pennarossa, Sharon Arcuri, Rolando Pasquariello, Fulvio Gandolfi, Margherita Maranesi, Tiziana A. L. Brevini
Summary: The potential antiviral effects of indole-3-carbinol (I3C), a phytochemical found in Cruciferous vegetables, were investigated in this study. 3D in vitro platforms mimicking the trachea and intestinal mucosae were used to evaluate the protective effect of I3C. The results showed that I3C can reduce the transcription of specific genes involved in the virus life cycle and may have the potential to prevent coronavirus infection.
Article
Biochemistry & Molecular Biology
Claudia Vanessa Arellano-Gutierrez, Laura Itzel Quintas-Granados, Hernan Cortes, Manuel Gonzalez del Carmen, Gerardo Leyva-Gomez, Lilia Patricia Bustamante-Montes, Miguel Rodriguez-Morales, Israel Lopez-Reyes, Juan Ramon Padilla-Mendoza, Lorena Rodriguez-Paez, Gabriela Figueroa-Gonzalez, Octavio Daniel Reyes-Hernandez
Summary: This study found that indole-3-carbinol can reduce the proliferation of cervical cancer cells by promoting the activation of AhR and inducing the expression of UBE2L3 mRNA, which may lead to the ubiquitination of HPV E7.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Rasha Z. Batran, Sherien M. El-Daly, Walaa A. El-Kashak, Eman Y. Ahmed
Summary: Two series of quinoline-thiazole and quinoline-thiazolidinone hybrids were synthesized and evaluated for their in vitro antitumor activity. Compound 4b and 6b showed better antiproliferative activity than lapatinib, with compound 4b also exhibiting better inhibitory activity towards the EGFR pathway. Compound 4b was capable of down-regulating anti-apoptotic genes and up-regulating a pro-apoptotic gene.
CHEMICAL BIOLOGY & DRUG DESIGN
(2022)
Article
Biochemistry & Molecular Biology
Bassem M. Raafat, Amira M. Gamal-Eldeen, Mazen M. Almehmadi, Sherien M. El-Daly, Nahla L. Faizo, Fayez Althobaiti
Summary: This study investigated the influence of living in high-altitude areas on rabbits' hemoglobin levels and antioxidant enzymes. It found that living in high-altitude areas led to abnormal hemoglobin levels and increased oxidative stress. However, supplementation with antioxidant-rich extracts such as Angelica archangelica and Ginkgo biloba helped restore normal hemoglobin levels and reduce oxidative stress.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2022)
Article
Environmental Sciences
Shaimaa A. Gouhar, Mahmoud T. Abo-Elfadl, Amira M. Gamal-Eldeen, Sherien M. El-Daly
Summary: The study revealed that alpha-solanine induces oxidative stress in human hepatocellular carcinoma HepG2 cells, mediated by alterations in the NF-kappa B pathway influencing the expression of oxidative stress-associated miRNAs.
ENVIRONMENTAL TOXICOLOGY
(2022)
Review
Pharmacology & Pharmacy
Sherien M. El-Daly, Shaimaa A. Gouhar, Zakaria Y. Abd Elmageed
Summary: This article reviews the potential applications of cell-free circulating miRNAs as noninvasive biomarkers in clinical settings, as well as the importance of integrating them with standard laboratory biomarkers to improve sensitivity and specificity.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2023)
Article
Oncology
Safinaz E. El-Toukhy, Sherien M. El-Daly, Mahmoud M. Kamel, Heba K. Nabih
Summary: This study investigated the expression patterns of circulating biomarkers in breast cancer patients and found that miRNA-145, miRNA-382, and miRNA-21 are associated with breast cancer development. These biomarkers could serve as non-invasive diagnostic tools for early prediction of breast cancer.
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
(2023)
Article
Medicine, Research & Experimental
Sherien M. El-Daly, Mahmoud T. Abo-elfadl, Jihan Hussein, Mona A. M. Abo-Zeid
Summary: This study investigates the impact of suppressing the activity of the PI3K/Akt/mTOR signaling pathway on the chemosensitivity of colorectal cancer cells to 5-FU. The results demonstrate that combining a low dose of 5-FU with a PI3K inhibitor can significantly enhance cytotoxicity and reduce the expression of specific drug transporters. Additionally, the recommended combination treatment induces cell apoptosis and reduces cell migration ability.
Article
Biochemistry & Molecular Biology
Sherien M. El-Daly, Mona A. El-Bana, Sahar S. Abd El-Rahman, Yasmin Abdel Latif, Dalia Medhat
Summary: This study aimed to investigate the expression profile of H19 and MALAT1 during different stages of hepatocarcinogenesis and their correlation with genes involved in carcinogenesis cascade. A murine model of chemically induced hepatocarcinogenesis was used. Real-time PCR was employed to analyze the expression patterns of H19, MALAT1, and epithelial-mesenchymal transition (EMT) biomarkers. The results showed a significant increase in the expression of H19 and MALAT1 throughout the stages compared to the normal control. Tumor progression biomarkers exhibited a similar increasing trend, while Zinc finger E-box-binding homeobox 1 and 2 (ZEB1 and ZEB2) showed a significant elevation only at the last stage of induction. The correlation analysis revealed a strong positive correlation between the expression pattern of H19 and MALAT1 with Matrix Metalloproteinases 2 and 9 and vimentin. This study suggests that genetic and epigenetic alterations contribute to hepatocellular carcinoma development in a stepwise progressive pattern.
CELL BIOCHEMISTRY AND FUNCTION
(2023)
Article
Multidisciplinary Sciences
Rares Drula, Barbara Pardini, Xiao Fu, Mireia Cruz De los Santos, Ancuta Jurj, Lan Pang, Sherien M. El-Daly, Linda Fabris, Erik Knutsen, Mihnea P. Dragomir, Recep Bayraktar, Yongfeng Li, Meng Chen, Filippo Del Vecchio, Lea Berland, Jessica Dae, Daniel Fan, Masayoshi Shimizu, Anh M. Tran, Mercedes Barzi, Carlotta Pioppini, Angelica M. Gutierrez, Cristina Ivan, Salyna Meas, Carolyn S. Hall, Suresh K. Alahari, Ioana Berindan-Neagoe, Muller Fabbri, Anthony Lucci, Banu Arun, Simone Anfossi, George A. Calin
Summary: The causes and consequences of abnormal biogenesis of extracellular vesicles (EVs) in breast cancers (BCs), especially estrogen receptor-positive (ER+) BCs, have been investigated. It was found that physiological doses of estrogen can promote EV secretion from ER+ BC cells by inhibiting miR-149-5p, which affects the regulatory activity of SP1 on the EV biogenesis factor nSMase2. In addition, the downregulation of miR-149-5p promotes the expression of hnRNPA1, responsible for loading let-7's miRNAs into EVs. This estrogen-driven mechanism leads to the elimination of tumor suppressor miRNAs in EVs, affecting tumor-associated macrophages in the microenvironment.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Mona A. El-Bana, Sherien M. El-Daly, Enayat A. Omara, Safaa M. Morsy, Mehrez E. El-Naggar, Dalia Medhat
Summary: As estrogen production decreases during menopause, the brain's metabolism becomes less effective, leading to the need for further research on hormone replacement therapy for neuroprotection. This study aimed to investigate the potential of pumpkin seed oil nanoparticles (PSO-NE) in attenuating neural-immune interactions in a postmenopausal model.
PROSTAGLANDINS & OTHER LIPID MEDIATORS
(2023)
Editorial Material
Biochemistry & Molecular Biology
Sherien M. El-Daly, Roba M. Talaat, Maria Braoudaki, Rana A. Youness, William C. Cho
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Hend M. Ahmed, Hanan H. Shehata, Gamila S. M. El-Saeed, Hoda H. Abou Gabal, Sherien M. El-Daly
Summary: The concept of paracetamol as a safe drug has been misleading, leading to a high rate of paracetamol toxicity. Oxidative stress plays an important role in paracetamol hepatotoxicity. The study investigates the mechanism of action of paracetamol-induced hepatotoxicity and warns against unsupervised use of paracetamol.
EGYPTIAN JOURNAL OF CHEMISTRY
(2023)
Article
Dermatology
Noha Abdel-Rahim Nagui, Marwa Saleh, Sherien El-Daly, Nohha Khater, Dina El Sharkawy
Summary: This study measured the relative expression levels of plasma vitamin D, vitamin D-binding protein (VDBP), and vitamin D receptor (VDR) in basal cell carcinoma (BCC) patients and healthy controls. The results showed significantly lower levels of vitamin D, VDBP, and VDR expression in BCC patients compared to controls, indicating a potential role in the pathogenesis of the disease. Furthermore, VDR relative expression was significantly lower in BCC tissue compared to nonlesional skin.
JOURNAL OF EGYPTIAN WOMENS DERMATOLOGICAL SOCIETY
(2022)
Article
Biotechnology & Applied Microbiology
Hend M. Ahmed, Hanan H. Shehata, Gamila S. M. El-Saeed, Hoda H. Abou Gabal, Sherien M. El-Daly
Summary: Our study demonstrates that probiotic LGG supplementation has a prophylactic and therapeutic effect against APAP-induced hepatotoxicity by modulating the expression of PKC and the Nrf2/PGC-1 alpha signaling pathway, ultimately suppressing oxidative damage caused by APAP overdose.
JOURNAL OF GENETIC ENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Sherien M. El-Daly, Shaimaa A. Gouhar, Mahmoud T. Abo-elfadl
Summary: This study aimed to investigate the dose-dependent effect of 5-fluorouracil (5-FU) on the expression of drug transporters and miRNAs in colorectal cancer. The results showed that a low dose of 5-FU upregulated the expression of drug transporters and miRNAs, while increasing the dose led to a decrease in expression.
EGYPTIAN JOURNAL OF CHEMISTRY
(2022)
Article
Nutrition & Dietetics
Amira M. Gamal-Eldeen, Bassem M. Raafat, Amani A. Alrehaili, Sherien M. El-Daly, Nahed Hawsawi, Hamsa J. Banjer, Eman M. Raafat, Mazen M. Almehmadi
Summary: This study found that the water-soluble polysaccharide fraction SD2 of Sargassum dentifolium has a potential anti-hypoxia effect, which may help in decreasing hypoxia-induced therapeutic resistance.
FRONTIERS IN NUTRITION
(2022)