Article
Chemistry, Multidisciplinary
Mathias Wiemann, Emil Axell, Henrik Stalbrand
Summary: The transglycosylation behavior of two GH-D clan alpha-galactosidases, BoGal36A and Aga27A, was studied using different glycosyl donors and acceptors. Aga27A showed better stability in the presence of acceptors, while BoGal36A had a higher transglycosylation rate at early time points but was more prone to secondary hydrolysis.
APPLIED SCIENCES-BASEL
(2022)
Article
Biotechnology & Applied Microbiology
Jingjing Sun, Congyu Yao, Yujie Li, Wei Wang, Jianhua Hao, Yi Yu
Summary: In this study, a novel β-galactosidase was isolated from a deep-sea metagenome, overexpressed in Escherichia coli, and purified. The enzyme showed high activity at specific temperatures and pH conditions, and exhibited resistance to salt. Moreover, it displayed hydrolysis and transglycosylation activity towards substrates such as oNPG and lactose.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Xin Shi, Dan Wu, Yan Xu, Xiaowei Yu
Summary: The study successfully shifted the pH optimum of lacA from acidic to neutral range using the mutant Y138F and Y364F, alleviating galactose inhibition and improving hydrolytic ability in milk.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Agronomy
Roger H. Tang, Peter D. Erskine, Philip Nti Nkrumah, Guillaume Echevarria, Antony van der Ent
Summary: Metallophytes are plants that can tolerate extreme metal concentrations in soil. A study on the Dugald River gossan in Australia found that there was no direct relationship between the occurrence of metallophytes and prevailing soil metal concentrations. Crotalaria novae-hollandiae was identified as a strong polymetallic indicator-type metallophyte that can accumulate high concentrations of metals in its leaves.
Review
Biotechnology & Applied Microbiology
Zuzana Meszaros, Pavlina Nekvasilova, Pavla Bojarova, Vladimir Kren, Kristyna Slamova
Summary: With the establishment of glycosynthase and transglycosidase technology by genetic engineering, glycosidases have become competitive synthetic tools in industrial biotechnology, offering advantages such as easy production, affordability, robustness, and substrate versatility.
BIOTECHNOLOGY ADVANCES
(2021)
Review
Biotechnology & Applied Microbiology
Zuzana Meszaros, Pavlina Nekvasilova, Pavla Bojarova, Vladimir Kren, Kristyna Slamova
Summary: Traditionally, glycosidases have been underutilized in industry, primarily used for cleaving unwanted carbohydrates, but with advancements in genetic engineering, they are now competitive synthetic tools capable of producing various chemicals, such as chitooligomers and glycosylated flavonoids. Exciting developments are on the horizon, including the production of human milk oligosaccharides and remodeled antibodies.
BIOTECHNOLOGY ADVANCES
(2021)
Article
Chemistry, Physical
Yafeng Bai, Kaidi Li, Liying Wang, Yang Gao, Xuesong Li, Xijia Yang, Wei Lu
Summary: In this study, a flexible zinc ion supercapacitor with gel electrolytes, porous alpha-MnO2@reduced graphene oxide cathode, and activated carbon/carbon cloth anode was developed. The device exhibits excellent electrochemical performance and stability, even at low temperatures, with a high cycle retention rate after 5000 cycles.
JOURNAL OF POWER SOURCES
(2024)
Article
Materials Science, Multidisciplinary
Soumya Kundu, Dongyang Zhang, Abdelrahman M. Askar, Erin G. Moloney, Michael M. Adachi, Ayesha Nadeem, Shahram Moradi, Vishal Yeddu, Ahmed L. Abdelhady, Oleksandr Voznyy, Makhsud Saidaminov
Summary: Stabilization of alpha-FAPbI3 single crystals is achieved through Pb-site doping with the heterovalent metal-bismuth (Bi), leading to an extended half-life of the alpha-to-delta phase transition. Density functional theory calculations suggest that Bi doping can improve the photovoltaic performance of Bi-doped alpha-FAPbI3-based solar cells.
ACS MATERIALS LETTERS
(2022)
Article
Oncology
Yi Zhang, Jinchuan Li, Xiaofeng Yin
Summary: This study found that GLA expression levels in LGG are associated with immune infiltration, which may have potential value for assessing tumor development.
Article
Construction & Building Technology
Hongtao Peng, Dong Wang, Massoud Sofi, Priyan Mendis, Zhiyuan Zhou, Junli Liu
Summary: Large volumes of lead-zinc tailings are being produced globally, and their disposal to landfills poses environmental problems. This study demonstrates the effective use of these tailings to produce ceramisite, which can be enhanced by adding clay. Factors such as sintering temperature, sintering time, and LZT content are found to significantly impact the properties of the ceramisite. Testing showed that the leaching content of hazardous components in the ceramisite samples was below the specified limit values, indicating their suitability for civil engineering applications.
JOURNAL OF MATERIALS IN CIVIL ENGINEERING
(2021)
Article
Biotechnology & Applied Microbiology
Yulin Zhang, Long Pan, Yue Zhang, Kaifang Wang, Liang Wang, Hongjian Zhang, Jianhua Zhang, Xusheng Chen
Summary: This study revealed the response mechanism of Streptomyces albulus to low-pH stress. It showed that S. albulus maintained intracellular pH homeostasis, increased unsaturated fatty acid ratio and extended fatty acid chain length. It also enhanced ATP accumulation, H+-ATPase activity, and accumulated basic amino acids L-lysine and L-arginine. At the transcription level, carbohydrate metabolism, oxidative phosphorylation, macromolecule protection and repair, and acid tolerance system were involved in combating low-pH stress. Gene manipulation was used to evaluate the effect of acid tolerance system and cell membrane fatty acid synthesis on low-pH tolerance.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Physics, Particles & Fields
Matteo Borghesi, Marco Faverzani, Cecilia Ferrari, Elena Ferri, Andrea Giachero, Angelo Nucciotti, Luca Origo
Summary: This study demonstrates that the dead time in experiments utilizing LTDs can be greatly reduced using the matrix optimum filtering approach.
EUROPEAN PHYSICAL JOURNAL C
(2022)
Article
Chemistry, Multidisciplinary
Yinglun Sun, Hongyun Ma, Xiaqing Zhang, Bao Liu, Lingyang Liu, Xu Zhang, Jianze Feng, Qingnuan Zhang, Yunxia Ding, Bingjun Yang, Liangti Qu, Xingbin Yan
Summary: This study introduces a new approach using Zn(ClO4)(2) salty ice as the electrolyte, achieving superior performance of a zinc ion hybrid capacitor even at low temperatures. This discovery provides a new insight for constructing low-temperature EES devices using salty ices as electrolytes.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Multidisciplinary Sciences
Abdolrahim Foroutan, Majid Abbas Zadeh Haji Abadi, Yaser Kianinia, Mahdi Ghadiri
Summary: The study investigated the impact of collector type and pulp pH on the lead-zinc ore flotation process, revealing that using the appropriate collector and optimal pH values can maximize lead and zinc purification.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Abiramy Krishnan, Zazali Alias, Peter Convey, Marcelo Gonzalez-Aravena, Jerzy Smykla, Mohammed Rizman-Idid, Siti Aisyah Alias
Summary: Understanding the role of fungi in soil ecosystems requires not only studying their diversity and enzyme activities, but also extracting and purifying target enzymes. This study obtained partially purified alpha-amylase from fungi strains collected in Antarctica and the Arctic, showing potential for biotechnological applications.
FERMENTATION-BASEL
(2022)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.