Article
Chemistry, Applied
Chen Zhou, Chengjun Sun, Haimin Zou, Yongxin Li
Summary: A plasma colorimetric aptasensor was developed for rapid determination of chloramphenicol in honey. Cage gold shell@core nanoparticles (Au@AuNPs) were synthesized to enhance signal response and broaden the linear range. In addition, an aptamer-based cascade hybridization chain reaction (cHCR) was designed for signal amplification and specific analysis. The aptasensor showed high sensitivity, good selectivity, low cost and excellent stability, making it a potential alternative tool in food analysis and environmental monitoring.
Article
Agriculture, Multidisciplinary
Liling Hao, Mengqiu Li, Kaimin Peng, Tai Ye, Xiuxiu Wu, Min Yuan, Hui Cao, Fengqin Yin, Huajie Gu, Fei Xu
Summary: This study proposes a simple and novel strategy to improve the sensitivity of FRET aptasensors by using DNA tetrahedron and gold nanorods. The method exhibits linear detection sensitivity in the concentration range of 0.01-10 ng/mL, with a limit of detection of 0.005 ng/mL. The developed biosensor demonstrates efficient specificity, good accuracy and stability, and performs well in real samples.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Jinshan Yuan, Guoming Xie, Junjie Li, Yaxing Xie, Yuan Yao, Jianhong Zhang, Yulei Hou, Hui Chen
Summary: A fluorescent sensing platform was developed for the simple and sensitive monitoring of vancomycin, using an allosteric probe that undergoes a conformational change in the presence of vancomycin. This platform has the potential to support therapeutic drug monitoring of vancomycin, promoting the rational use of antibiotics in clinics.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Yao Yao, Jingyu Kuang, Jiahe Ju, Tao Hu, Wei Shen, Sheng Tang, Chang Liu, Dezhao Kong, Haiwei Shi, Hian Kee Lee
Summary: A simple fluorescence sensing device based on solvent-free magnetic-tip microextraction was developed and successfully applied for the fluorescence detection of chloramphenicol (CAP) in genuine milk and urine samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Zhijun Huang, Naizhi Yao, Xiaoting Li, Yonghui Tian, Yixiang Duan
Summary: The SEIA system, based on self-extending DNA-mediated isothermal amplification, provides a simple and efficient way to achieve rapid, robust, and significant signal amplification, demonstrating potential for broad-spectrum target detection in various scenarios.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Wei Li, Ding Zhao, Dongdong Tian, Manman Zhai, Hongdan Xu, Liuchuang Zheng, Shengqiang Li, Ye Sang
Summary: The article introduces an enzyme-free electrochemical strategy for analyzing thrombin concentration in human serum using a DNA network assembled by nonlinear hybridization chain reaction. The strategy has high sensitivity and specificity, and performs well in detecting thrombin in serum samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Xiaoyan He, Jiandi Dong, Huimin Han, Nan Sun, Wenyu Shi, Xiong Lu, Hui Jia, Xiaoquan Lu
Summary: The newly designed electrochemical aptasensor utilizing DNA-templated copolymers is capable of sensitive detection of ATP, with a wide linear response range and high selectivity, achieving a detection limit as low as 11.5 fM.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Food Science & Technology
Li Zou, Manjun Zhang, Mengyan Li, Zhiyou Xiao, Liansheng Ling
Summary: This study developed an enzyme-free fluorescent aptasensor using a dual signal amplification strategy for sensitive detection of aflatoxin B1. The aptasensor showed high sensitivity and selectivity, making it suitable for food safety control.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Chemistry, Applied
Ge Wu, Zhengwei Xiong, Suk-Heung Oh, Yanrong Ren, Qiang Wang, Lizhu Yang
Summary: A two-color fluorescent DNA tweezers developed for ultrasensitive detection of OTA shows a good linear range and detection limit, effectively reducing false positive rate in practical food samples.
Article
Chemistry, Multidisciplinary
Xiyuan Yu, Wei Lin, Qian Lai, Juan Song, Yilong Liu, Rui Su, Qi Niu, Liu Yang, Chaoyong Yang, Huimin Zhang, Zhi Zhu
Summary: In this study, an Armor strategy was proposed to protect mammalian cell integrity from environmental changes. The Armor was created through dual-aptamer-based hybridization chain reaction (HCR) and Ca2+ assisted calcification for selective cell protection.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Analytical
Xuelan Wu, Ting Ju, Zeyang Li, Jingwen Li, Xingwei Zhai, Kun Han
Summary: This study designed and optimized a target-independent fluorescent signal system, Hybridization chain reaction Fluorescence resonance energy transfer (HCR-FRET) system. Combined with CRISPR/Cas12a system, a fluorescent biosensing protocol was developed for the detection of T790 M. The HCR-FRET system showed a detection range from 1 pM to 400 pM with a detection limit of 316 fM. The target-independent property of the HCR-FRET system indicates its potential for parallel detection of other DNA targets.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Zheng Wei Wong, Siu Yee New
Summary: A fluorescence biosensor based on hybridisation chain reaction amplification coupled with silver nanoclusters has been developed for nucleic acid detection, providing a reliable and low risk method for detecting target DNA.
Article
Chemistry, Analytical
Junyang Wang, Hongxia Li, Caiyi Du, Ying Li, Xinyue Ma, Chuanyu Yang, Wentao Xu, Chunyan Sun
Summary: A novel aptasensor for tobramycin was successfully constructed using hybridization chain reaction (HCR) and fluorescence synergism. The aptasensor showed low detection limit and could monitor tobramycin in a wide concentration range. The amplification of fluorescence signal was achieved through HCR and the released cDNA-FAM acted as an initiator to trigger the amplification. Graphene oxide effectively quenched the background signal, enabling sensitive and specific detection of tobramycin.
Article
Chemistry, Multidisciplinary
Xueqin Yang, Lijiao Yang, Donglei Yang, Min Li, Pengfei Wang
Summary: Cell membrane proteins play a crucial role in regulating intracellular signal transductions and cell behaviors. By utilizing membrane-anchored DNA hybridization chain reaction, DNA self-assembly can be induced on the live cell surface, leading to the clustering of membrane proteins and modulation of cell behaviors.
Article
Chemistry, Multidisciplinary
Bowen Zhang, Taoran Tian, Dexuan Xiao, Shaojingya Gao, Xiaoxiao Cai, Yunfeng Lin
Summary: A DNA-enhanced HCR detection system (T-probe system) is introduced for the detection of cancer-related targets, demonstrating its versatility and efficiency by detecting miRNA-21 and nucleolin. The T-probe system shows high detection rate and rapid recognition of both membrane and intracellular cancer-related targets, and exhibits superior fluorescence for imaging tumor-related biomarkers in vitro and in vivo, indicating its great potential for clinical diagnosis and therapeutic applications.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.