Article
Chemistry, Analytical
Manassawee Janrod, Monpichar Srisa-Art
Summary: A microfluidic paper-based analytical device (mu PAD) with colorimetric detection was developed for simultaneous determination of urinary oxalate, citrate, and uric acid. The detection methods utilized enzymatic reactions and an indicator displacement assay. The mu PAD showed promising results with linear ranges and low detection limits for the three biomarkers, as well as acceptable accuracy and precision in urine sample testing. This development holds great potential for on-site analysis, providing a simple, fast, affordable, and portable tool for simultaneous determination of the biomarkers.
ANALYTICAL METHODS
(2023)
Article
Multidisciplinary Sciences
Subhashis Patari, Priyankan Datta, Pallab Sinha Mahapatra
Summary: This study developed a three-dimensional microfluidic device capable of simultaneously detecting multiple chemical adulterants in milk, requiring only 1-2 mL of sample volume to detect 7 adulterants at once. The simple, portable, and user-friendly device is expected to be used for testing liquid foods in resource-limited settings.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Xinya Zhang, Juan Qiao, Wei Liu, Li Qi
Summary: Gold nanoclusters (AuNCs)-based nanozymes with L-tryptophanonitrile protection show enhanced peroxidase-like activity through aggregation, leading to a colorimetric method for highly specific and sensitive detection of oxytetracycline. The study demonstrates the potential of AuNCs-based nanozymes in real bio-applications.
Article
Biochemistry & Molecular Biology
Javier Hoyo, Arnau Bassegoda, Guillem Ferreres, Dolores Hinojosa-Caballero, Manuel Gutierrez-Capitan, Antoni Baldi, Cesar Fernandez-Sanchez, Tzanko Tzanov
Summary: Current procedures for assessing chronic wound infection are time-consuming and require complex instruments and trained personnel. This study developed a foldable paper-based device for detecting the enzyme myeloperoxidase (MPO) in wound fluids, which is a suitable biomarker for wound infection diagnosis. The device showed efficient analytic performance in ex vivo experiments and correlated strongly with a spectrophotometric assay.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Applied
Ana Carolina Rafanhin Sousa, Cleyton Nascimento Makara, Lais Canniatti Brazaca, Emanuel Carrilho
Summary: In order to detect sulfonamides in milk, a simple and portable microfluidic paper-based analytical device (mu PAD) was developed for precise analysis without the need for pretreatment. This device has good linearity and selectivity, potentially allowing for on-site monitoring of milk quality to reduce adverse conditions associated with high concentrations of sulfonamides.
Article
Chemistry, Analytical
Tania Pomili, Paolo Donati, Pier Paolo Pompa
Summary: The study presents a monolithic and fully integrated paper-based device for the simultaneous detection of three prognostic biomarkers in saliva. The device features a unique design and simple fabrication process, with particles exhibiting visible color changes at non-physiological concentrations.
Article
Chemistry, Analytical
Bangxiang Liu, Hengjia Zhu, Rulin Feng, Mengzhu Wang, Panwang Hu, Jianming Pan, Xiangheng Niu
Summary: In this study, molecularly imprinted sites were introduced onto the Fe3O4 nanozyme surface to develop a highly specific colorimetric assay for tetracycline detection. The self-polymerization of dopamine was employed to construct Fe3O4 @ MIP, which showed abundant channels for substrate access and achieved a detection limit of 0.4 μ M. The assay demonstrated high selectivity for tetracycline against structural analogues, making it suitable for practical applications in environmental water matrices.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Arezoo Saadati, Fatemeh Farshchi, Mohammad Hasanzadeh, Farzad Seidi
Summary: The study introduced a sensitive, quantitative, and rapid diagnostic method for uric acid using silver nanoparticles (AgNPs), suggesting the potential use of various types of AgNPs for on-site detection of clinical samples and biomarkers with portable devices.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Applied
Komal Birader, Pankaj Kumar, Yathirajarao Tammineni, Jeannie Alice Barla, Shashidhar Reddy, Pankaj Suman
Summary: An aptamer has been developed to detect the banned antibiotic Oxytetracycline (OTC) in veterinary practice, and its interaction with OTC has been confirmed through molecular modelling and circular dichroism spectroscopy. A lateral flow-based aptasensor has been successfully developed to visually detect OTC in spiked milk within 10 minutes, showing no cross reactivity with milk components and correlating well with HPLC analysis.
Article
Chemistry, Analytical
Inmaculada Ortiz-Gomez, Almudena Rivadeneyra, Jose F. Salmeron, Ignacio de Orbe-Paya, Diego P. Morales, Luis Fermin Capitan-Vallvey, Alfonso Salinas-Castillo
Summary: In this study, a microfluidic paper-based analytical device (mu PAD) with an NFC tag was proposed as a portable, simple and fast colorimetric method for glutathione (GSH) determination. The method took advantage of the oxidizing property of Ag+ on 3,3';,5,5'-tetramethylbenzidine (TMB), which resulted in a blue color fading when GSH was present. By integrating electronic interfaces into the hardware of digital image capture, a smartphone was utilized for the colorimetric determination of GSH. Importantly, this new method exhibited a low detection limit of 1.0 mu M, offering high sensitivity and a simple, fast, portable and low-cost GSH determination within 20 minutes using a colorimetric signal.
Article
Chemistry, Analytical
Parthena Chorti, Abbas Parvez Kazi, Michael Wiederoder, Dionysios C. Christodouleas
Summary: This study introduces a novel flow-through direct immunoassay (FTDI) methodology for targeted detection of bacteria in liquid samples, providing rapid and sensitive detection, as well as ensuring accuracy and precision in various types of samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Md Sadique Hasan, Monireh Marsafari, Michael Tolosa, Abhay Andar, Sai Sathish Ramamurthy, Xudong Ge, Yordan Kostov, Govind Rao
Summary: A system featuring microfluidics and a multichannel fluorometer has been developed to detect low-level bioburden. The system allows parallel testing of multiple samples with a low limit of detection. By monitoring the fluorescence intensity, the presence of viable cells can be detected. The system is portable, low-cost, and has high assay sensitivity.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Raouia Attaallah, Aziz Amine
Summary: The research developed a colorimetric method based on the inhibition of HRP enzyme and a paper-based analytical device integrated with a smartphone platform for rapid and quantitative detection of Cd2+ in drinking water. The method has a low limit of detection of 0.1 ppb and showed good selectivity, making it a valuable tool for monitoring cadmium ions in water.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Zhichao Yu, Hexiang Gong, Fangqin Xue, Yongyi Zeng, Xiaolong Liu, Dianping Tang
Summary: In this study, a flexible and low-cost thermosensitive fiber paper was developed for visual display in photothermal biosensing systems for early acute myocardial infarction. The color change mechanism of the thermal paper was based on the temperature-driven reversible transformation of the dye molecule. The results provide new insights for the development of low-cost, instrument-free portable photothermal biosensors.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Analytical
Giacomo Musile, Yvane Agard, Sara Pesavento, Elio F. De Palo, Romolo M. Dorizzi, Federica Bortolotti
Summary: This study developed a simple and low-cost urine testing device for on-site screening of urine dilution. The device utilized a smartphone camera and associated software for color detection and data processing, enabling quick and accurate determination of creatinine levels in urine, thereby indicating the presence of urine tampering.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Applied
M. Graca Dias, Elsa Vasco, Francisco Ravasco, Lufsa Oliveira
Summary: This study estimated the vitamin D intake of "adults" and "elderly" populations in Portugal using the TDS methodology. The results showed that the majority of people had inadequate vitamin D intake, well below the Dietary Reference Values.
Article
Chemistry, Applied
Yanan Wang, Jiachen Shi, Yong-Jiang Xu, Chin-Ping Tan, Yuanfa Liu
Summary: This study investigates the variations in lipid digestion profiles among individuals of different ages using in vitro digestion models. The findings suggest that adults have a more comprehensive lipid digestion compared to infants, and infants tend to release shorter chain length and more saturated free fatty acids during digestion. Additionally, the particle sizes in the stomach of the elderly were consistently larger. This study enhances our understanding of how lipids with different degrees of unsaturation undergo digestion in diverse age groups.
Article
Chemistry, Applied
Hyunjong Yu, Huisu Kim, Pahn-Shick Chang
Summary: Chitosan-pectin emulsion-filled hydrogel (EFH) was developed to enhance the bioaccessibility of lipophilic bioactive compounds through intestinal delivery. The EFH, prepared without crosslinking agents, demonstrated improved mechanical strength and compactness with higher pectin concentration. It retained the emulsion at pH 2.0 and released it at pH 7.4, resulting in enhanced release of free fatty acids and improved bioaccessibility of curcumin.
Article
Chemistry, Applied
Tongze Zhang, Siqi Hong, Jia-Rong Zhang, Pin-He Liu, Siyi Li, Zixian Wen, Jianwei Xiao, Guirong Zhang, Olivier Habimana, Nagendra P. Shah, Zhongquan Sui, Harold Corke
Summary: Lactic acid fermentation significantly affects the morphology and physicochemical properties of proso millet starch, including the formation of surface indentations and small pores, decrease in gelatinization temperatures, and changes in hardness and adhesiveness.
Article
Chemistry, Applied
Liqin Kong, Feng Hong, Peng Luan, Yiping Chen, Yaoze Feng, Ming Zhu
Summary: This study presents a novel impedance biosensor using composite nanomaterials and T1R1 as a signal probe, which can competitively and ultra-sensitively detect umami intensity. The biosensor exhibits exceptional analytical performance and is suitable for food flavor evaluation.
Article
Chemistry, Applied
Kunying Lv, Yixin Yang, Qilong Li, Ran Chen, Liang Deng, Yiwei Zhang, Ning Jiang
Summary: Horse's milk, with its high nutritional value and low allergenic proteins, could be a substitute for cow's milk in infant consumption. A proteomic method was used to identify and compare milk fat globule membrane (MFGM) and whey proteins from different horse breeds. The study found differences in protein composition and functionality, which could support the development of formulas more suitable for human infants.
Article
Chemistry, Applied
Enrique Jacobo Diaz-Montana, Helene Brignot, Ramon Aparicio-Ruiz, Thierry Thomas- Danguin, Maria Teresa Morales
Summary: Sensory perception of virgin olive oil is influenced by phenols and volatiles, which are affected by the composition of the oil and biological factors. This study investigated the effect of saliva and phenols on the release of volatiles, and found that the presence of phenols decreased the release of saturated volatiles.
Article
Chemistry, Applied
Wei Zhou, Rui Zhang, Zhen Cai, Fangfang Wu, Yong Hu, Chao Huang, Kun Hu, Yun Chen
Summary: Environmentally friendly and outstanding pH-responsive cationic starch nanoparticles (CSNP) were prepared from pH-sensitive starch. CSNP exhibited nanosize and regular sphere, highly free-flowing molecular chains, and demonstrated excellent pH responsiveness through multiple emulsion/demulsification transitions.
Article
Chemistry, Applied
Andrea Koo, Vinayak Ghate, Weibiao Zhou
Summary: This study suggests that direct seeding may negatively affect the nutritional quality of crops, causing a decrease in ascorbic acid, vitamin K, and total glucosinolate content.
Article
Chemistry, Applied
Tonghao Du, Yazhou Xu, Xiaoyan Xu, Shijin Xiong, Linli Zhang, Biao Dong, Jinqing Huang, Tao Huang, Muyan Xiao, Tao Xiong, Mingyong Xie
Summary: This study successfully improved the ACE inhibitory activity of black sesame seeds by fermenting them with Lactobacillus Plantarum NCU116 and hydrolyzing them using acid protease. The RF-PSO model was used to predict the ACE inhibitory activity during the hydrolysis process. Eight peptides with ACE inhibitory activity were identified from fermented black sesame seed hydrolysates after separation and screening.
Article
Chemistry, Applied
Yao Liu, Songyi Lin, Kexin Liu, Shan Wang, Qiaozhen Liu, Na Sun
Summary: This study analyzed the structural changes of shrimp proteins during digestion, predicted the immunodominant epitopes, and validated their allergenicity. The results showed that shrimp proteins were degraded into peptides during digestion, but still carried IgE epitopes that trigger allergic reactions.
Article
Chemistry, Applied
Tiantian Fu, Hongwei Cao, Yu Zhang, Xiao Guan
Summary: This study investigates the impact of milling on the active components in rice, with a focus on the stability and bioaccessibility of phenols, VB1, and alpha-GABA during cooking and digestion. The findings show that milling exacerbates the instability of gamma-GABA during cooking and VB1 during digestion, and it affects the bioaccessibility of these active compounds.
Article
Chemistry, Applied
Zhihao Yang, Yanru Hou, Min Zhang, Puxin Hou, Chang Liu, Lu Dou, Xiaoyu Chen, Lihua Zhao, Lin Su, Ye Jin
Summary: This study investigated the molecular mechanism of feeding regimes on lamb flavor by using TMT labeling combined with MS. The results showed that pasture-fed groups had higher levels of amino acids and volatile flavor substances compared to concentrate-fed groups. Additionally, several differentially abundant proteins associated with lamb flavor were identified.
Article
Chemistry, Applied
Zixuan Xie, De Zhang, Junyu Zhu, Qianqian Luo, Jun Liu, Jingtao Zhou, Xiaoyong Wang, Yuqiong Chen, Zhi Yu, Dejiang Ni
Summary: This study investigated the acidification of aroma-enhanced black tea during storage. Analysis of non-volatile substances and organic acids using UPLC-Q-TOF/MS and HPLC revealed a decrease in soluble sugars and amino acids, while an increase in organic acids such as oxalic acid, malic acid, and quinic acid. In vitro experiments further demonstrated that the acidification is a result of the decomposition of sugars and amino acids by heating, as well as the oxidation of aromatic aldehydes. Additionally, the study showed that the taste composition of tea infusion is altered, with reduced amino acids, catechins, soluble sugars, and flavonoids. This research provides a theoretical basis for improving the quality of black tea.
Article
Chemistry, Applied
Lin Wang, Falai Ma, Zihan Li, Yan Zhang
Summary: This study developed time-temperature integrators based on amyloglucosidase@Cu3(PO4)2 nanoflowers for monitoring the freshness of chilled pork. The results showed that the integrators were highly reliable and accurate in predicting the quality of chilled pork.