Dismantling the bacterial glycocalyx: Chemical tools to probe, perturb, and image bacterial glycans

Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome

(2021) Zhen Han et al.   Scientific Reports

A glycosylated cationic block poly(beta-peptide) reverses intrinsic antibiotic resistance in all ESKAPE Gram-negative bacteria

(2020) Zhangyong Si et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Mammalian lectin arrays for screening host-microbe interactions

(2020) Sabine A. F. Jégouzo et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Maltotriose-based probes for fluorescence and photoacoustic imaging of bacterial infections

(2020) Aimen Zlitni et al.   Nature Communications

Distinct cytoskeletal proteins define zones of enhanced cell wall synthesis in Helicobacter pylori

(2020) Jennifer A Taylor et al.   eLife

Photoactivatable Glycolipid Probes for Identifying Mycolate–Protein Interactions in Live Mycobacteria

(2020) Herbert W. Kavunja et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A comparative review of intelectins

(2020) Lei Chen et al.   SCANDINAVIAN JOURNAL OF IMMUNOLOGY

Small molecule sensors targeting the bacterial cell wall

(2020) Matthew F. L. Parker et al.   ACS Infectious Diseases

Fluorogenic d-amino acids enable real-time monitoring of peptidoglycan biosynthesis and high-throughput transpeptidation assays

(2019) Yen-Pang Hsu et al.   Nature Chemistry

Identification of the Functional Roles of Six Key Proteins in the Biosynthesis of Enterobacteriaceae Colanic Acid

(2019) Phillip M. Scott et al.   BIOCHEMISTRY

Antibacterial Small Molecules That Potently Inhibit Staphylococcus aureus Lipoteichoic Acid Biosynthesis

(2019) George A. Naclerio et al.   ChemMedChem

Bacterial cell wall modification with a glycolipid substrate

(2019) Phillip Calabretta et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Phasevarions of bacterial pathogens – phase-variable epigenetic regulators evolving from restriction–modification systems

(2019) Zachary N. Phillips et al.   MICROBIOLOGY-SGM

Rhamnose Binding Protein as an Anti-Bacterial Agent—Targeting Biofilm of Pseudomonas aeruginosa

(2019) Tse-Kai Fu et al.   Marine Drugs

Investigation of the Binding Affinity of a Broad Array of l-Fucosides with Six Fucose-Specific Lectins of Bacterial and Fungal Origin

(2019) Son Thai Le et al.   MOLECULES

d-Amino Acid Derivatives as in Situ Probes for Visualizing Bacterial Peptidoglycan Biosynthesis

(2019) Yen-Pang Hsu et al.   ACCOUNTS OF CHEMICAL RESEARCH

Mechanisms of Incorporation for D-Amino Acid Probes That Target Peptidoglycan Biosynthesis

(2019) Erkin Kuru et al.   ACS Chemical Biology

BabA and LPS inhibitors against Helicobacter pylori: pectins and pectin-like rhamnogalacturonans as adhesion blockers

(2019) Maren Gottesmann et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Bacterial glycans and their interactions with lectins in the innate immune system

(2019) Mariano Prado Acosta et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Structure and genetics of Escherichia coli O antigens

(2019) Bin Liu et al.   FEMS MICROBIOLOGY REVIEWS

Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed β-propeller fold

(2019) Petra Sýkorová et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

A mutation in the glycosyltransferase gene lafB causes daptomycin hypersusceptibility in Enterococcus faecium

(2019) Suelen S Mello et al.   JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY

Imaging Bacterial Cell Wall Biosynthesis

(2018) Atanas D. Radkov et al.   Annual Review of Biochemistry

Proximity labeling: spatially resolved proteomic mapping for neurobiology

(2018) Shuo Han et al.   CURRENT OPINION IN NEUROBIOLOGY

Host-bacteria interaction and adhesin study for development of therapeutics

(2018) Vandana Solanki et al.   INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Concise Chemoenzymatic Synthesis of Trehalosamine, an Aminoglycoside Antibiotic and Precursor to Mycobacterial Imaging Probes

(2018) Jessica M Groenevelt et al.   JOURNAL OF ORGANIC CHEMISTRY

Synthesis of functionalized N-acetyl muramic acids to probe bacterial cell wall recycling and biosynthesis

(2018) Kristen E. DeMeester et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Streptomyces coelicolor strains lacking polyprenol phosphate mannose synthase and protein O-mannosyl transferase are hyper-susceptible to multiple antibiotics

(2018) Robert Howlett et al.   MICROBIOLOGY-SGM

Imaging mycobacterial growth and division with a fluorogenic probe

(2018) Heather L. Hodges et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Fluorescent Antibiotics: New Research Tools to Fight Antibiotic Resistance

(2018) M. Rhia L. Stone et al.   TRENDS IN BIOTECHNOLOGY

Cell-based glycan arrays for probing glycan–glycan binding protein interactions

(2018) Jennie Grace Briard et al.   Nature Communications

Metabolic Labeling of Pseudaminic Acid-containing Glycans on Bacterial Surfaces

(2018) gloria andolina et al.   ACS Chemical Biology

Facile Solid-Phase Synthesis and Assessment of Nucleoside Analogs as Inhibitors of Bacterial UDP-Sugar Processing Enzymes

(2018) Amaël G. E. Madec et al.   ACS Chemical Biology

Zebrafish intelectin 1 (zITLN1) plays a role in the innate immune response

(2018) Lei Chen et al.   FISH & SHELLFISH IMMUNOLOGY

Lectins as antimicrobial agents

(2018) L.C. Breitenbach Barroso Coelho et al.   JOURNAL OF APPLIED MICROBIOLOGY

Chemical Synthesis of Rare, Deoxy-Amino Sugars Containing Bacterial Glycoconjugates as Potential Vaccine Candidates

(2018) Archanamayee Behera et al.   MOLECULES

Peptidoglycan precursor synthesis along the sidewall of pole-growing mycobacteria

(2018) Alam García-Heredia et al.   eLife

The Fluorescent D-Amino Acid NADA as a Tool to Study the Conditional Activity of Transpeptidases in Escherichia coli

(2018) Alejandro Montón Silva et al.   Frontiers in Microbiology

Chemoenzymatic radiosynthesis of 2-deoxy-2-[18F]fluoro-d-trehalose ([18F]-2-FDTre): A PET radioprobe for in vivo tracing of trehalose metabolism

(2018) Santiago Peña-Zalbidea et al.   CARBOHYDRATE RESEARCH

Synthesis and in Vitro Characterization of Trehalose-Based Inhibitors of Mycobacterial Trehalose 6-Phosphate Phosphatases

(2018) Sunayana Kapil et al.   CHEMBIOCHEM

Screening of a Library of Oligosaccharides Targeting Lectin LecB of Pseudomonas Aeruginosa and Synthesis of High Affinity Oligoglycoclusters

(2018) Lucie Dupin et al.   MOLECULES

In situ identification of Gram-negative bacteria in human lungs using a topical fluorescent peptide targeting lipid A

(2018) Ahsan R. Akram et al.   Science Translational Medicine

Differential recognition of Haemophilus influenzae whole bacterial cells and isolated lipooligosaccharides by galactose-specific lectins

(2018) Ioanna Kalograiaki et al.   Scientific Reports

Maturing Mycobacterium smegmatis peptidoglycan requires non-canonical crosslinks to maintain shape

(2018) Catherine Baranowski et al.   eLife

Targeting Bacillosamine Biosynthesis in Bacterial Pathogens: Development of Inhibitors to a Bacterial Amino-Sugar Acetyltransferase from Campylobacter jejuni

(2017) Joris W. De Schutter et al.   JOURNAL OF MEDICINAL CHEMISTRY

Amphiphilic Tobramycin–Lysine Conjugates Sensitize Multidrug Resistant Gram-Negative Bacteria to Rifampicin and Minocycline

(2017) Yinfeng Lyu et al.   JOURNAL OF MEDICINAL CHEMISTRY

Development of IsoTaG, a Chemical Glycoproteomics Technique for Profiling Intact N- and O-Glycopeptides from Whole Cell Proteomes

(2017) Christina M. Woo et al.   JOURNAL OF PROTEOME RESEARCH

Visualization of mycobacterial membrane dynamics in live cells

(2017) Frances P. Rodriguez-Rivera et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A semisynthetic Streptococcus pneumoniae serotype 8 glycoconjugate vaccine

(2017) Benjamin Schumann et al.   Science Translational Medicine

Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls

(2017) Yen-Pang Hsu et al.   Chemical Science

Strategies for combating bacterial biofilms: A focus on anti-biofilm agents and their mechanisms of action

(2017) Ranita Roy et al.   Virulence

Lipoprotein Glycosylation by Protein-O-Mannosyltransferase (MAB_1122c) Contributes to Low Cell Envelope Permeability and Antibiotic Resistance of Mycobacterium abscessus

(2017) Katja Becker et al.   Frontiers in Microbiology

Illuminating vital surface molecules of symbionts in health and disease

(2017) Jason E. Hudak et al.   Nature Microbiology

Norgestimate inhibits staphylococcal biofilm formation and resensitizes methicillin-resistant Staphylococcus aureus to β-lactam antibiotics

(2017) Yutaka Yoshii et al.   npj Biofilms and Microbiomes

Complete Tetrasaccharide Repeat Unit Biosynthesis of the Immunomodulatory Bacteroides fragilis Capsular Polysaccharide A

(2016) Sunita Sharma et al.   ACS Chemical Biology

Development of Rare Bacterial Monosaccharide Analogs for Metabolic Glycan Labeling in Pathogenic Bacteria

(2016) Emily L. Clark et al.   ACS Chemical Biology

Isotope-targeted glycoproteomics (IsoTaG) analysis of sialylated N- and O-glycopeptides on an Orbitrap Fusion Tribrid using azido and alkynyl sugars

(2016) Christina M. Woo et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Combined Bacteria Microarray and Quartz Crystal Microbalance Approach for Exploring Glycosignatures of Nontypeable Haemophilus influenzae and Recognition by Host Lectins

(2016) Ioanna Kalograiaki et al.   ANALYTICAL CHEMISTRY

Bioorthogonal Chemical Reporters for Selective In Situ Probing of Mycomembrane Components in Mycobacteria

(2016) Hannah N. Foley et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Peptidoglycan microarray as a novel tool to explore protein-ligand recognition

(2016) Ning Wang et al.   BIOPOLYMERS

Getting a grip on glycans: A current overview of the metabolic oligosaccharide engineering toolbox

(2016) Tjerk J. Sminia et al.   CARBOHYDRATE RESEARCH

Quantitative profiling of glycans and glycopeptides: an informatics’ perspective

(2016) Ian Walsh et al.   CURRENT OPINION IN STRUCTURAL BIOLOGY

A zebrafish intelectin ortholog agglutinates both Gram-negative and Gram-positive bacteria with binding capacity to bacterial polysaccharide

(2016) Lei Chen et al.   FISH & SHELLFISH IMMUNOLOGY

Structures ofXenopusEmbryonic Epidermal Lectin Reveal a Conserved Mechanism of Microbial Glycan Recognition

(2016) Kittikhun Wangkanont et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Quantitation of Polymyxin–Lipopolysaccharide Interactions Using an Image-Based Fluorescent Probe

(2016) Mitchell P. McInerney et al.   JOURNAL OF PHARMACEUTICAL SCIENCES

Expedient Route To Access Rare Deoxy Amino l-Sugar Building Blocks for the Assembly of Bacterial Glycoconjugates

(2016) Someswara Rao Sanapala et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Progress and prospects for small-molecule probes of bacterial imaging

(2016) Ozden Kocaoglu et al.   Nature Chemical Biology

Mycobacterial cell wall biosynthesis: a multifaceted antibiotic target

(2016) KATHERINE A. ABRAHAMS et al.   PARASITOLOGY

Multivalent display of minimal Clostridium difficile glycan epitopes mimics antigenic properties of larger glycans

(2016) Felix Broecker et al.   Nature Communications

Pathogenic Chlamydia Lack a Classical Sacculus but Synthesize a Narrow, Mid-cell Peptidoglycan Ring, Regulated by MreB, for Cell Division

(2016) George Liechti et al.   PLoS Pathogens

The Logic of Automated Glycan Assembly

(2015) Peter H. Seeberger   ACCOUNTS OF CHEMICAL RESEARCH

Role of Capsular Polysaccharides in Biofilm Formation: An AFM Nanomechanics Study

(2015) Huabin Wang et al.   ACS Applied Materials & Interfaces

Assembly of the Mycobacterial Cell Wall

(2015) Monika Jankute et al.   Annual Review of Microbiology

Antigenic Potential of a Highly Conserved Neisseria meningitidis Lipopolysaccharide Inner Core Structure Defined by Chemical Synthesis

(2015) Anika Reinhardt et al.   CHEMISTRY & BIOLOGY

A Modular Approach to Phosphoglycosyltransferase Inhibitors Inspired by Nucleoside Antibiotics

(2015) Marthe T. C. Walvoort et al.   CHEMISTRY-A EUROPEAN JOURNAL

Investigation of the protective properties of glycosylphosphatidylinositol-based vaccine candidates in aToxoplasma gondiimouse challenge model

(2015) Sebastian Götze et al.   GLYCOBIOLOGY

Minimal Peptidoglycan (PG) Turnover in Wild-Type and PG Hydrolase and Cell Division Mutants of Streptococcus pneumoniae D39 Growing Planktonically and in Host-Relevant Biofilms

(2015) Michael J. Boersma et al.   JOURNAL OF BACTERIOLOGY

d-Amino Acid Probes for Penicillin Binding Protein-based Bacterial Surface Labeling

(2015) Jonathan M. Fura et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

In vivo imaging and tracking of host–microbiota interactions via metabolic labeling of gut anaerobic bacteria

(2015) Naama Geva-Zatorsky et al.   NATURE MEDICINE

Isotope-targeted glycoproteomics (IsoTaG): a mass-independent platform for intact N- and O-glycopeptide discovery and analysis

(2015) Christina M Woo et al.   NATURE METHODS

Bacteria microarrays as sensitive tools for exploring pathogen surface epitopes and recognition by host receptors

(2015) María Asunción Campanero-Rhodes et al.   RSC Advances

d-Amino Acid Mediated Recruitment of Endogenous Antibodies to Bacterial Surfaces

(2014) Jonathan M. Fura et al.   ACS Chemical Biology

A fluorescent carbapenem for structure function studies of penicillin-binding proteins, β-lactamases, and β-lactam sensors

(2014) Cynthia M. June et al.   ANALYTICAL BIOCHEMISTRY

Glycans in pathogenic bacteria – potential for targeted covalent therapeutics and imaging agents

(2014) Van N. Tra et al.   CHEMICAL COMMUNICATIONS

Tackling vancomycin-resistant bacteria with ‘lipophilic–vancomycin–carbohydrate conjugates’

(2014) Venkateswarlu Yarlagadda et al.   JOURNAL OF ANTIBIOTICS

Detection of Lipid-Linked Peptidoglycan Precursors by Exploiting an Unexpected Transpeptidase Reaction

(2014) Yuan Qiao et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Bacteria Fighting Back: How Pathogens Target and Subvert the Host Innate Immune System

(2014) L. Evan Reddick et al.   MOLECULAR CELL

NMR of glycans: Shedding new light on old problems

(2014) Marcos D. Battistel et al.   PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY

Peptidoglycan Synthesis Machinery in Agrobacterium tumefaciens During Unipolar Growth and Cell Division

(2014) T. A. Cameron et al.   mBio

Automated Solid-Phase Synthesis of Chondroitin Sulfate Glycosaminoglycans

(2013) Steffen Eller et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

N-Glycosylation of Campylobacter jejuni Surface Proteins Promotes Bacterial Fitness

(2013) Abofu Alemka et al.   INFECTION AND IMMUNITY

A new metabolic cell-wall labelling method reveals peptidoglycan in Chlamydia trachomatis

(2013) G. W. Liechti et al.   NATURE

Proteomic Mapping of Mitochondria in Living Cells via Spatially Restricted Enzymatic Tagging

(2013) H.-W. Rhee et al.   SCIENCE

d-Amino Acid Chemical Reporters Reveal Peptidoglycan Dynamics of an Intracellular Pathogen

(2012) M. Sloan Siegrist et al.   ACS Chemical Biology

In Situ Probing of Newly Synthesized Peptidoglycan in Live Bacteria with FluorescentD-Amino Acids

(2012) Erkin Kuru et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

A promiscuous biotin ligase fusion protein identifies proximal and interacting proteins in mammalian cells

(2012) Kyle J. Roux et al.   JOURNAL OF CELL BIOLOGY

Probing the Mycobacterial Trehalome with Bioorthogonal Chemistry

(2012) Benjamin M. Swarts et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

A Decade of Bioorthogonal Chemistry

(2011) Carolyn R. Bertozzi   ACCOUNTS OF CHEMICAL RESEARCH

Biochemical Characterization of the O-Linked Glycosylation Pathway inNeisseria gonorrhoeaeResponsible for Biosynthesis of Protein Glycans ContainingN,N′-Diacetylbacillosamine

(2011) Meredith D. Hartley et al.   BIOCHEMISTRY

Cell surface glycoproteomic analysis of prostate cancer-derived PC-3 cells

(2011) Sarah C. Hubbard et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Metabolic labeling of fucosylated glycoproteins in Bacteroidales species

(2011) Christen Besanceney-Webler et al.   BIOORGANIC & MEDICINAL CHEMISTRY LETTERS

Evaluation of 4,5-Disubstituted-2-Aminoimidazole-Triazole Conjugates for Antibiofilm/Antibiotic Resensitization Activity Against MRSA and Acinetobacter baumannii

(2011) Zhaoming Su et al.   ChemMedChem

Chemical reporters for fluorescent detection and identification of O-GlcNAc-modified proteins reveal glycosylation of the ubiquitin ligase NEDD4-1

(2011) B. W. Zaro et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Systems analysis of bacterial glycomes: Figure 1

(2010) Emily Kay et al.   BIOCHEMICAL SOCIETY TRANSACTIONS

Chemical tools to discover and target bacterial glycoproteins

(2010) Danielle H. Dube et al.   CHEMICAL COMMUNICATIONS

Antibiotic resistance of bacterial biofilms

(2010) Niels Høiby et al.   INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS

Structural investigation of bacterial lipopolysaccharides by mass spectrometry and tandem mass spectrometry

(2010) Joseph H. Banoub et al.   MASS SPECTROMETRY REVIEWS

Copper-free click chemistry in living animals

(2010) P. V. Chang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

The Bacterial Cell Envelope

(2010) T. J. Silhavy et al.   Cold Spring Harbor Perspectives in Biology

Bioorthogonal Chemistry: Fishing for Selectivity in a Sea of Functionality

(2009) Ellen M. Sletten et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Bioanalytical tools for the discovery of eukaryotic glycoproteins applied to the analysis of bacterial glycoproteins

(2009) Lucie Balonova et al.   Expert Review of Proteomics

Lectins as pattern recognition molecules: The effects of epitope density in innate immunity

(2009) T. K Dam et al.   GLYCOBIOLOGY

Campylobacter jejuni Glycosylation Island Important in Cell Charge, Legionaminic Acid Biosynthesis, and Colonization of Chickens

(2009) S. L. Howard et al.   INFECTION AND IMMUNITY

Enrichment of glycopeptides for glycan structure and attachment site identification

(2009) Jonas Nilsson et al.   NATURE METHODS

Pseudomonas aeruginosa Exploits Lipid A and Muropeptides Modification as a Strategy to Lower Innate Immunity during Cystic Fibrosis Lung Infection

(2009) Cristina Cigana et al.   PLoS One

Siglecs as targets for therapy in immune-cell-mediated disease

(2009) Mary K. O’Reilly et al.   TRENDS IN PHARMACOLOGICAL SCIENCES

Galectin-1 signaling in leukocytes requires expression of complex-type N-glycans

(2008) S. Karmakar et al.   GLYCOBIOLOGY

Tetrazine Ligation: Fast Bioconjugation Based on Inverse-Electron-Demand Diels−Alder Reactivity

(2008) Melissa L. Blackman et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

On-line separations combined with MS for analysis of glycosaminoglycans

(2008) Joseph Zaia   MASS SPECTROMETRY REVIEWS

A simple strategy for the creation of a recombinant lectin microarray

(2008) Ku-Lung Hsu et al.   Molecular BioSystems