Strategies for the Site-Specific Decoration of DNA Origami Nanostructures with Functionally Intact Proteins

DNA origami demonstrate the unique stimulatory power of single pMHCs as T cell antigens

(2021) Joschka Hellmeier et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Spatial Regulation of T-Cell Signaling by Programmed Death-Ligand 1 on Wireframe DNA Origami Flat Sheets

(2021) Trixy Fang et al.   ACS Nano

Advancing Biophysics Using DNA Origami

(2021) Wouter Engelen et al.   Annual Review of Biophysics

Temporal analysis of T-cell receptor-imposed forces via quantitative single molecule FRET measurements

(2021) Janett Göhring et al.   Nature Communications

Nanoscale FasL Organization on DNA Origami to Decipher Apoptosis Signal Activation in Cells

(2021) Ricarda M. L. Berger et al.   Small

Membrane Association Transforms an Inert Anti-TCRβ Fab’ Ligand into a Potent T Cell Receptor Agonist

(2020) Jenny J. Lin et al.   BIOPHYSICAL JOURNAL

Role of nanoscale antigen organization on B-cell activation probed using DNA origami

(2020) Rémi Veneziano et al.   Nature Nanotechnology

Unscrambling fluorophore blinking for comprehensive cluster detection via photoactivated localization microscopy

(2020) René Platzer et al.   Nature Communications

A DNA nanorobot functions as a cancer therapeutic in response to a molecular trigger in vivo

(2018) Suping Li et al.   NATURE BIOTECHNOLOGY

Monomeric TCRs drive T cell antigen recognition

(2018) Mario Brameshuber et al.   NATURE IMMUNOLOGY

Full control of ligand positioning reveals spatial thresholds for T cell receptor triggering

(2018) Haogang Cai et al.   Nature Nanotechnology

Quantifying absolute addressability in DNA origami with molecular resolution

(2018) Maximilian T. Strauss et al.   Nature Communications

DNA Origami Nanoarrays for Multivalent Investigations of Cancer Cell Spreading with Nanoscale Spatial Resolution and Single-Molecule Control

(2018) Da Huang et al.   ACS Nano

Enhanced Efficiency of an Enzyme Cascade on DNA-Activated Silica Surfaces

(2018) Kilian Vogele et al.   LANGMUIR

Orthogonal Protein Assembly on DNA Nanostructures Using Relaxases

(2016) Sandra Sagredo et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Designed DNA Surfaces for in Vitro Modulation of Natural Killer Cells

(2016) Ruben Garrecht et al.   CHEMBIOCHEM

A Rationally Designed Connector for Assembly of Protein-Functionalized DNA Nanostructures

(2016) Katja J. Koßmann et al.   CHEMBIOCHEM

Proximity does not contribute to activity enhancement in the glucose oxidase–horseradish peroxidase cascade

(2016) Yifei Zhang et al.   Nature Communications

Assembly and Purification of Enzyme-Functionalized DNA Origami Structures

(2015) Christopher Timm et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Multiscale Origami Structures as Interface for Cells

(2015) Alessandro Angelin et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay

(2015) Markus Axmann et al.   Jove-Journal of Visualized Experiments

Orthogonal enzyme arrays on a DNA origami scaffold bearing size-tunable wells

(2014) Takahiro Yamazaki et al.   Nanoscale

Spatial control of membrane receptor function using ligand nanocalipers

(2014) Alan Shaw et al.   NATURE METHODS

Multi-enzyme complexes on DNA scaffolds capable of substrate channelling with an artificial swinging arm

(2014) Jinglin Fu et al.   Nature Nanotechnology

Plug-and-Play Pairing via Defined Divalent Streptavidins

(2013) Michael Fairhead et al.   JOURNAL OF MOLECULAR BIOLOGY

Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures

(2013) Na Wu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Interenzyme Substrate Diffusion for an Enzyme Cascade Organized on Spatially Addressable DNA Nanostructures

(2012) Jinglin Fu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Synthetic Lipid Membrane Channels Formed by Designed DNA Nanostructures

(2012) M. Langecker et al.   SCIENCE

Orthogonal Protein Decoration of DNA Origami

(2010) Barbara Saccà et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Imaging of Mobile Long-lived Nanoplatforms in the Live Cell Plasma Membrane

(2010) Mario Brameshuber et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

NUPACK: Analysis and design of nucleic acid systems

(2010) Joseph N. Zadeh et al.   JOURNAL OF COMPUTATIONAL CHEMISTRY

TCR–peptide–MHC interactions in situ show accelerated kinetics and increased affinity

(2010) Johannes B. Huppa et al.   NATURE

Single-molecule chemical reactions on DNA origami

(2010) Niels V. Voigt et al.   Nature Nanotechnology

Tuning of Peroxidase Activity by Covalently Tethered DNA Oligonucleotides

(2009) Maximilian Glettenberg et al.   BIOCONJUGATE CHEMISTRY

Accurate detection and complete tracking of large populations of features in three dimensions

(2009) Yongxiang Gao et al.   OPTICS EXPRESS

HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis

(2008) Georgyi V. Los et al.   ACS Chemical Biology