Role of nanoscale antigen organization on B-cell activation probed using DNA origami

Binding to nanopatterned antigens is dominated by the spatial tolerance of antibodies

(2019) Alan Shaw et al.   Nature Nanotechnology

Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus

(2019) Jessica Marcandalli et al.   CELL

Programming Structured DNA Assemblies to Probe Biophysical Processes

(2019) Eike-Christian Wamhoff et al.   Annual Review of Biophysics

Antigen Receptor Function in the Context of the Nanoscale Organization of the B Cell Membrane

(2019) Michael R. Gold et al.   Annual Review of Immunology

Bioproduction of pure, kilobase-scale single-stranded DNA

(2019) Tyson R. Shepherd et al.   Scientific Reports

A generalized HIV vaccine design strategy for priming of broadly neutralizing antibody responses

(2019) Jon M. Steichen et al.   SCIENCE

Enhancing and shaping the immunogenicity of native-like HIV-1 envelope trimers with a two-component protein nanoparticle

(2019) Philip J. M. Brouwer et al.   Nature Communications

Rapid Germinal Center and Antibody Responses in Non-human Primates after a Single Nanoparticle Vaccine Immunization

(2019) Colin Havenar-Daughton et al.   Cell Reports

Targeted selection of HIV-specific antibody mutations by engineering B cell maturation

(2019) Kevin O. Saunders et al.   SCIENCE

Precursor Frequency and Affinity Determine B Cell Competitive Fitness in Germinal Centers, Tested with Germline-Targeting HIV Vaccine Immunogens

(2018) Robert K. Abbott et al.   IMMUNITY

The human naive B cell repertoire contains distinct subclasses for a germline-targeting HIV-1 vaccine immunogen

(2018) Colin Havenar-Daughton et al.   Science Translational Medicine

In vitro synthesis of gene-length single-stranded DNA

(2018) Rémi Veneziano et al.   Scientific Reports

Germline-targeting immunogens

(2017) Leonidas Stamatatos et al.   IMMUNOLOGICAL REVIEWS

The nanoscale spatial organization of B-cell receptors on immunoglobulin M– and G–expressing human B-cells

(2017) Jinmin Lee et al.   MOLECULAR BIOLOGY OF THE CELL

Protein sorting by lipid phase-like domains supports emergent signaling function in B lymphocyte plasma membranes

(2017) Matthew B Stone et al.   eLife

HIV Vaccine Design to Target Germline Precursors of Glycan-Dependent Broadly Neutralizing Antibodies

(2016) Jon M. Steichen et al.   IMMUNITY

In vitro reconstitution of B cell receptor–antigen interactions to evaluate potential vaccine candidates

(2016) Grant C Weaver et al.   Nature Protocols

Priming HIV-1 broadly neutralizing antibody precursors in human Ig loci transgenic mice

(2016) D. Sok et al.   SCIENCE

HIV-1 broadly neutralizing antibody precursor B cells revealed by germline-targeting immunogen

(2016) J. G. Jardine et al.   SCIENCE

Designer nanoscale DNA assemblies programmed from the top down

(2016) R. Veneziano et al.   SCIENCE

Antigen Receptor Nanoclusters: Small Units with Big Functions

(2016) Wanli Liu et al.   TRENDS IN IMMUNOLOGY

Reconstituted B cell receptor signaling reveals carbohydrate-dependent mode of activation

(2016) Rina F. Villar et al.   Scientific Reports

Multivalent Antigens for Promoting B and T Cell Activation

(2015) Nitasha R. Bennett et al.   ACS Chemical Biology

Programmable motion of DNA origami mechanisms

(2015) Alexander E. Marras et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Priming a broadly neutralizing antibody response to HIV-1 using a germline-targeting immunogen

(2015) J. G. Jardine et al.   SCIENCE

B cell activation involves nanoscale receptor reorganizations and inside-out signaling by Syk

(2014) Kathrin Kläsener et al.   eLife

Rational HIV Immunogen Design to Target Specific Germline B Cell Receptors

(2013) J. Jardine et al.   SCIENCE

Monovalent and Multivalent Ligation of the B Cell Receptor Exhibit Differential Dependence upon Syk and Src Family Kinases

(2013) S. Mukherjee et al.   Science Signaling

NIH Image to ImageJ: 25 years of image analysis

(2012) Caroline A Schneider et al.   NATURE METHODS

Early Events in B Cell Activation

(2010) Naomi E. Harwood et al.   Annual Review of Immunology

Low-valency, but not monovalent, antigens trigger the B-cell antigen receptor (BCR)

(2010) S. Minguet et al.   INTERNATIONAL IMMUNOLOGY

Antigen affinity discrimination is an intrinsic function of the B cell receptor

(2010) Wanli Liu et al.   JOURNAL OF EXPERIMENTAL MEDICINE

Oligomeric organization of the B-cell antigen receptor on resting cells

(2010) Jianying Yang et al.   NATURE

The Constant Region of the Membrane Immunoglobulin Mediates B Cell-Receptor Clustering and Signaling in Response to Membrane Antigens

(2009) Pavel Tolar et al.   IMMUNITY