Emerging methods in biomarker identification for extracellular vesicle‐based liquid biopsy

Multi-functional detection of extracellular vesicles with surface plasmon resonance microscopy

(2020) YUTING YANG et al.   ANALYTICAL CHEMISTRY

Thermophoretic Detection of Exosomal microRNAs by Nanoflares

(2020) Junxiang Zhao et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

From laboratory to clinic: Translation of extracellular vesicle based cancer biomarkers

(2020) Anudeep Yekula et al.   METHODS

MIFlowCyt-EV: a framework for standardized reporting of extracellular vesicle flow cytometry experiments

(2020) Joshua A. Welsh et al.   Journal of Extracellular Vesicles

Label-free identification and chemical characterisation of single extracellular vesicles and lipoproteins by synchronous Rayleigh and Raman scattering

(2020) Agustin Enciso-Martinez et al.   Journal of Extracellular Vesicles

Extracellular vesicle-based EGFR genotyping in bronchoalveolar lavage fluid from treatment-naive non-small cell lung cancer patients

(2020) Jae Young Hur et al.   Translational Lung Cancer Research

Early-Stage Lung Cancer Diagnosis by Deep Learning-Based Spectroscopic Analysis of Circulating Exosomes

(2020) Hyunku Shin et al.   ACS Nano

RNA delivery by extracellular vesicles in mammalian cells and its applications

(2020) Killian O’Brien et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Clinical utility of the exosome based ExoDx Prostate(IntelliScore) EPI test in men presenting for initial Biopsy with a PSA 2–10 ng/mL

(2020) Ronald Tutrone et al.   PROSTATE CANCER AND PROSTATIC DISEASES

Enrichment of plasma extracellular vesicles for reliable quantification of their size and concentration for biomarker discovery

(2020) Marija Holcar et al.   Scientific Reports

Molecular Recognition-Based DNA Nanodevices to Enhance the Direct Visualization and Quantification of Single-Vesicles of Tumor Exosomes in Plasma Microsamples

(2019) Dinggeng He et al.   ANALYTICAL CHEMISTRY

High-fidelity probing of the structure and heterogeneity of extracellular vesicles by resonance-enhanced atomic force microscopy infrared spectroscopy

(2019) Sally Yunsun Kim et al.   Nature Protocols

Subtyping of circulating exosome-bound amyloid β reflects brain plaque deposition

(2019) Carine Z. J. Lim et al.   Nature Communications

Quantitative and Specific Detection of Exosomal miRNAs for Accurate Diagnosis of Breast Cancer Using a Surface-Enhanced Raman Scattering Sensor Based on Plasmonic Head-Flocked Gold Nanopillars

(2019) Jong Uk Lee et al.   Small

Mobile platform for rapid sub–picogram-per-milliliter, multiplexed, digital droplet detection of proteins

(2019) Venkata Yelleswarapu et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

λ-DNA- and Aptamer-Mediated Sorting and Analysis of Extracellular Vesicles

(2019) Chao Liu et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Ultrasensitive immunoprofiling of plasma extracellular vesicles identifies syndecan-1 as a potential tool for minimally invasive diagnosis of glioma

(2019) Vineesh Indira Chandran et al.   CLINICAL CANCER RESEARCH

Droplet digital PCR improves urinary exosomal miRNA detection compared to real-time PCR

(2019) Cong Wang et al.   CLINICAL BIOCHEMISTRY

Near-Infrared Afterglow Semiconducting Nano-Polycomplexes for the Multiplex Differentiation of Cancer Exosomes

(2019) Yan Lyu et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Label-free detection of exosomes using a surface plasmon resonance biosensor

(2019) Abu Ali Ibn Sina et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

Ultrasensitive detection of circulating exosomes with a 3D-nanopatterned microfluidic chip

(2019) Peng Zhang et al.   Nature Biomedical Engineering

Super-resolution microscopy as a powerful tool to study complex synthetic materials

(2019) Silvia Pujals et al.   Nature Reviews Chemistry

Rapid Detection of Exosomal MicroRNAs Using Virus-Mimicking Fusogenic Vesicles

(2019) Xihui Gao et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Dual-SERS biosensor for one-step detection of microRNAs in exosome and residual plasma of blood samples for diagnosing pancreatic cancer

(2019) Yuanfeng Pang et al.   BIOSENSORS & BIOELECTRONICS

A microfluidic chip enables isolation of exosomes and establishment of their protein profiles and associated signaling pathways in ovarian cancer

(2019) Kalpana Deepa Priya Dorayappan et al.   CANCER RESEARCH

Reassessment of Exosome Composition

(2019) Dennis K. Jeppesen et al.   CELL

Cell death: a review of the major forms of apoptosis, necrosis and autophagy

(2019) Mark S D’Arcy   CELL BIOLOGY INTERNATIONAL

A Sensitive Aptasensor Based on a Hemin/G‐Quadruplex‐Assisted Signal Amplification Strategy for Electrochemical Detection of Gastric Cancer Exosomes

(2019) Rongrong Huang et al.   Small

Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles

(2019) Mikołaj Piotr Zaborowski et al.   Cell Reports

Transfer of Functional Cargo in Exomeres

(2019) Qin Zhang et al.   Cell Reports

MALDI Detection of Exosomes: A Potential Tool for Cancer Studies

(2019) Yingdi Zhu et al.   Chem

Preparation of a Novel Raman Probe and Its Application in the Detection of Circulating Tumor Cells and Exosomes

(2019) Xiaoru Zhang et al.   ACS Applied Materials & Interfaces

Direct quantification of cancerous exosomes via surface plasmon resonance with dual gold nanoparticle-assisted signal amplification

(2019) Qing Wang et al.   BIOSENSORS & BIOELECTRONICS

The generation and use of recombinant extracellular vesicles as biological reference material

(2019) Edward Geeurickx et al.   Nature Communications

Proteomic profiling of extracellular vesicles reveals additional diagnostic biomarkers for myocardial infarction compared to plasma alone

(2019) Olof Gidlöf et al.   Scientific Reports

Extracellular vesicle measurements with nanoparticle tracking analysis – An accuracy and repeatability comparison between NanoSight NS300 and ZetaView

(2019) Daniel Bachurski et al.   Journal of Extracellular Vesicles

Mapping Subpopulations of Cancer Cell-Derived Extracellular Vesicles and Particles by Nano-Flow Cytometry

(2019) Dongsic Choi et al.   ACS Nano

Profile of neuronal exosomes in HIV cognitive impairment exposes sex differences

(2019) Bing Sun et al.   AIDS

Electrical and Label-Free Quantification of Exosomes with a Reduced Graphene Oxide Field Effect Transistor Biosensor

(2019) Yi Yu et al.   ANALYTICAL CHEMISTRY

A catalytic molecule machine-driven biosensing method for amplified electrochemical detection of exosomes

(2019) Ya Cao et al.   BIOSENSORS & BIOELECTRONICS

Electrochemical Detection of Tumor-Derived Extracellular Vesicles on Nanointerdigitated Electrodes

(2019) Dilu G. Mathew et al.   NANO LETTERS

Absolute quantification and analysis of extracellular vesicle lncRNAs from the peripheral blood of patients with lung cancer based on multi-colour fluorescence chip-based digital PCR

(2019) Yanan Bai et al.   BIOSENSORS & BIOELECTRONICS

MicroRNA detection on microsensor arrays by SPR imaging measurements with enzymatic signal enhancement

(2019) Hiroshi Aoki et al.   BIOSENSORS & BIOELECTRONICS

Discrimination of urinary exosomes from microvesicles by lipidomics using thin layer liquid chromatography (TLC) coupled with MALDI-TOF mass spectrometry

(2019) Nilubon Singhto et al.   Scientific Reports

Extracellular vesicles and coagulation in blood from healthy humans revisited

(2019) René J. Berckmans et al.   Journal of Extracellular Vesicles

Synchronized Rayleigh and Raman scattering for the characterization of single optically trapped extracellular vesicles

(2019) Agustin Enciso-Martinez et al.   Nanomedicine-Nanotechnology Biology and Medicine

Protein Profiling and Sizing of Extracellular Vesicles from Colorectal Cancer Patients via Flow Cytometry

(2018) Ye Tian et al.   ACS Nano

Design and Clinical Verification of Surface Enhanced Raman Spectroscopy Diagnostic Technology for Individual Cancer Risk Prediction

(2018) Kevin Maisheng Koo et al.   ACS Nano

Detection and Characterization of Different Brain-Derived Subpopulations of Plasma Exosomes by Surface Plasmon Resonance Imaging

(2018) Silvia Picciolini et al.   ANALYTICAL CHEMISTRY

Frequency Shift Raman-Based Sensing of Serum MicroRNAs for Early Diagnosis and Discrimination of Primary Liver Cancers

(2018) Wen-Feng Zhu et al.   ANALYTICAL CHEMISTRY

Quantitative detection of exosomal microRNA extracted from human blood based on surface-enhanced Raman scattering

(2018) Dandan Ma et al.   BIOSENSORS & BIOELECTRONICS

A DNA–linker–DNA bifunctional probe for simultaneous SERS detection of miRNAs via symmetric signal amplification

(2018) Sujuan Ye et al.   CHEMICAL COMMUNICATIONS

Detection of Aggregation-Competent Tau in Neuron-Derived Extracellular Vesicles

(2018) Francesc Guix et al.   INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Hollow organosilica beads as reference particles for optical detection of extracellular vesicles

(2018) Z. Varga et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Subpopulations of extracellular vesicles and their therapeutic potential

(2018) Cecilia Lässer et al.   MOLECULAR ASPECTS OF MEDICINE

Identification of distinct nanoparticles and subsets of extracellular vesicles by asymmetric flow field-flow fractionation

(2018) Haiying Zhang et al.   NATURE CELL BIOLOGY

Understanding the diversity of membrane lipid composition

(2018) Takeshi Harayama et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Neuronal lysosomal dysfunction releases exosomes harboring APP C-terminal fragments and unique lipid signatures

(2018) André M. Miranda et al.   Nature Communications

Molecular Detection and Analysis of Exosomes Using Surface-Enhanced Raman Scattering Gold Nanorods and a Miniaturized Device

(2018) Elyahb Allie Kwizera et al.   Theranostics

Multiplex detection of urinary miRNA biomarkers by transmission surface plasmon resonance

(2018) Wing Kiu Yeung et al.   ANALYST

Unique Lipid Signatures of Extracellular Vesicles from the Airways of Asthmatics

(2018) Kenneth P. Hough et al.   Scientific Reports

MicroRNA sensors based on gold nanoparticles

(2018) Catarina Coutinho et al.   ANALYTICAL AND BIOANALYTICAL CHEMISTRY

ExoAPP: Exosome-Oriented, Aptamer Nanoprobe-Enabled Surface Proteins Profiling and Detection

(2018) Dan Jin et al.   ANALYTICAL CHEMISTRY

Nanoscale extracellular vesicle-derived DNA is superior to circulating cell-free DNA for mutation detection in early-stage non-small-cell lung cancer

(2018) Y Wan et al.   ANNALS OF ONCOLOGY

Ultrasensitive detection of miRNA with an antimonene-based surface plasmon resonance sensor

(2018) Tianyu Xue et al.   Nature Communications

Impact of preanalytical conditions on plasma concentration and size distribution of extracellular vesicles using Nanoparticle Tracking Analysis

(2018) Simin Jamaly et al.   Scientific Reports

Isolation of High-Purity Extracellular Vesicles by the Combination of Iodixanol Density Gradient Ultracentrifugation and Bind-Elute Chromatography From Blood Plasma

(2018) Zsófia Onódi et al.   Frontiers in Physiology

Correlation between Cancerous Exosomes and Protein Markers Based on Surface-Enhanced Raman Spectroscopy (SERS) and Principal Component Analysis (PCA)

(2018) Hyunku Shin et al.   ACS Sensors

Label-free characterization of exosome via surface enhanced Raman spectroscopy for the early detection of pancreatic cancer

(2018) Joseph Carmicheal et al.   Nanomedicine-Nanotechnology Biology and Medicine

Molecular lipid species in urinary exosomes as potential prostate cancer biomarkers

(2017) Tore Skotland et al.   EUROPEAN JOURNAL OF CANCER

Biological reference materials for extracellular vesicle studies

(2017) S. Valkonen et al.   EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES

Imaging flow cytometry for the characterization of extracellular vesicles

(2017) Joanne Lannigan et al.   METHODS

Tracing Cellular Origin of Human Exosomes Using Multiplex Proximity Extension Assays

(2017) Pia Larssen et al.   MOLECULAR & CELLULAR PROTEOMICS

EV-TRACK: transparent reporting and centralizing knowledge in extracellular vesicle research

(2017) Jan Van Deun et al.   NATURE METHODS

Lipids in exosomes: Current knowledge and the way forward

(2017) Tore Skotland et al.   PROGRESS IN LIPID RESEARCH

Multiparametric plasma EV profiling facilitates diagnosis of pancreatic malignancy

(2017) Katherine S. Yang et al.   Science Translational Medicine

A signal-amplifiable biochip quantifies extracellular vesicle-associated RNAs for early cancer detection

(2017) Jiaming Hu et al.   Nature Communications

A Cell-Surface Membrane Protein Signature for Glioblastoma

(2017) Dhimankrishna Ghosh et al.   Cell Systems

Nanoplasmonic quantification of tumour-derived extracellular vesicles in plasma microsamples for diagnosis and treatment monitoring

(2017) Kai Liang et al.   Nature Biomedical Engineering

Labeling Extracellular Vesicles for Nanoscale Flow Cytometry

(2017) Aizea Morales-Kastresana et al.   Scientific Reports

Raman spectroscopy uncovers biochemical tissue-related features of extracellular vesicles from mesenchymal stromal cells

(2017) Alice Gualerzi et al.   Scientific Reports

Integrated Magneto–Electrochemical Sensor for Exosome Analysis

(2016) Sangmoo Jeong et al.   ACS Nano

CNS tau efflux via exosomes is likely increased in Parkinson's disease but not in Alzheimer's disease

(2016) Min Shi et al.   Alzheimers & Dementia

Electrochemical Sandwich Immunosensor for Determination of Exosomes Based on Surface Marker-Mediated Signal Amplification

(2016) Ximena Doldán et al.   ANALYTICAL CHEMISTRY

Simultaneous and multiplexed detection of exosome microRNAs using molecular beacons

(2016) Ji Hye Lee et al.   BIOSENSORS & BIOELECTRONICS

Extracellular Vesicles in Cancer: Cell-to-Cell Mediators of Metastasis

(2016) Annette Becker et al.   CANCER CELL

Lipid synthesis and membrane contact sites: a crossroads for cellular physiology

(2016) J. Pedro Fernández-Murray et al.   JOURNAL OF LIPID RESEARCH

ExoCarta: A Web-Based Compendium of Exosomal Cargo

(2016) Shivakumar Keerthikumar et al.   JOURNAL OF MOLECULAR BIOLOGY

Identification of Individual Exosome-Like Vesicles by Surface Enhanced Raman Spectroscopy

(2016) Stephan Stremersch et al.   Small

Cells release subpopulations of exosomes with distinct molecular and biological properties

(2016) Eduard Willms et al.   Scientific Reports

Low-density lipoprotein mimics blood plasma-derived exosomes and microvesicles during isolation and detection

(2016) Barbara W Sódar et al.   Scientific Reports

Digital Detection of Exosomes by Interferometric Imaging

(2016) George G. Daaboul et al.   Scientific Reports

Label-Free Nanoplasmonic-Based Short Noncoding RNA Sensing at Attomolar Concentrations Allows for Quantitative and Highly Specific Assay of MicroRNA-10b in Biological Fluids and Circulating Exosomes

(2015) Gayatri K. Joshi et al.   ACS Nano

Identification of preclinical Alzheimer's disease by a profile of pathogenic proteins in neurally derived blood exosomes: A case-control study

(2015) Massimo S. Fiandaca et al.   Alzheimers & Dementia

In situ single step detection of exosome microRNA using molecular beacon

(2015) Ji Hye Lee et al.   BIOMATERIALS

High sensitivity flow cytometry of membrane vesicles

(2015) Samuel A. Stoner et al.   CYTOMETRY PART A

Prerequisites for the analysis and sorting of extracellular vesicle subpopulations by high-resolution flow cytometry

(2015) Tom Groot Kormelink et al.   CYTOMETRY PART A

Fluorescence triggering: A general strategy for enumerating and phenotyping extracellular vesicles by flow cytometry

(2015) Nicolas Arraud et al.   CYTOMETRY PART A

On-chip surface acoustic wave lysis and ion-exchange nanomembrane detection of exosomal RNA for pancreatic cancer study and diagnosis

(2015) Daniel Taller et al.   LAB ON A CHIP

Rapid and comprehensive ‘shotgun’ lipidome profiling of colorectal cancer cell derived exosomes

(2015) Todd A. Lydic et al.   METHODS

Improved Characterization of EV Preparations Based on Protein to Lipid Ratio and Lipid Properties

(2015) Xabier Osteikoetxea et al.   PLoS One

Chip-based analysis of exosomal mRNA mediating drug resistance in glioblastoma

(2015) Huilin Shao et al.   Nature Communications

EVpedia: a community web portal for extracellular vesicles research

(2014) D.-K. Kim et al.   BIOINFORMATICS

ComplexN-Linked Glycans Serve as a Determinant for Exosome/Microvesicle Cargo Recruitment

(2014) Yaxuan Liang et al.   JOURNAL OF BIOLOGICAL CHEMISTRY

Extracellular vesicles from blood plasma: determination of their morphology, size, phenotype and concentration

(2014) N. Arraud et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Particle size distribution of exosomes and microvesicles determined by transmission electron microscopy, flow cytometry, nanoparticle tracking analysis, and resistive pulse sensing

(2014) E. van der Pol et al.   JOURNAL OF THROMBOSIS AND HAEMOSTASIS

Refractive Index Determination of Nanoparticles in Suspension Using Nanoparticle Tracking Analysis

(2014) Edwin van der Pol et al.   NANO LETTERS

Label-free detection and molecular profiling of exosomes with a nano-plasmonic sensor

(2014) Hyungsoon Im et al.   NATURE BIOTECHNOLOGY

Homogenous 96-Plex PEA Immunoassay Exhibiting High Sensitivity, Specificity, and Excellent Scalability

(2014) Erika Assarsson et al.   PLoS One

Analysis of Extracellular RNA by Digital PCR

(2014) Kenji Takahashi et al.   Frontiers in Oncology

Molecular lipidomics of exosomes released by PC-3 prostate cancer cells

(2013) Alicia Llorente et al.   BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS

Extracellular vesicles: Exosomes, microvesicles, and friends

(2013) Graça Raposo et al.   JOURNAL OF CELL BIOLOGY

Localized surface plasmon resonance: a unique property of plasmonic nanoparticles for nucleic acid detection

(2013) Kah Ee Fong et al.   Nanoscale

Lipids of mitochondria

(2013) Susanne E. Horvath et al.   PROGRESS IN LIPID RESEARCH

Distinct lipid compositions of two types of human prostasomes

(2013) Jos F. Brouwers et al.   PROTEOMICS

BEAMing and Droplet Digital PCR Analysis of Mutant IDH1 mRNA in Glioma Patient Serum and Cerebrospinal Fluid Extracellular Vesicles

(2013) Walter W Chen et al.   Molecular Therapy-Nucleic Acids

Membrane Vesicle Release in Bacteria, Eukaryotes, and Archaea: a Conserved yet Underappreciated Aspect of Microbial Life

(2012) Brooke L. Deatherage et al.   INFECTION AND IMMUNITY

Label-Free Detection of Micro-RNA Hybridization Using Surface-Enhanced Raman Spectroscopy and Least-Squares Analysis

(2012) Justin L. Abell et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Protein typing of circulating microvesicles allows real-time monitoring of glioblastoma therapy

(2012) Huilin Shao et al.   NATURE MEDICINE

Fluorescent labeling of nano-sized vesicles released by cells and subsequent quantitative and qualitative analysis by high-resolution flow cytometry

(2012) Els J van der Vlist et al.   Nature Protocols

Simultaneous Detection of Single Molecules and Singulated Ensembles of Molecules Enables Immunoassays with Broad Dynamic Range

(2011) David M. Rissin et al.   ANALYTICAL CHEMISTRY

Homogeneous antibody-based proximity extension assays provide sensitive and specific detection of low-abundant proteins in human blood

(2011) Martin Lundberg et al.   NUCLEIC ACIDS RESEARCH

Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations

(2010) David M Rissin et al.   NATURE BIOTECHNOLOGY

Ceramide Triggers Budding of Exosome Vesicles into Multivesicular Endosomes

(2008) K. Trajkovic et al.   SCIENCE