CRISPR gRNA phenotypic screening in zebrafish reveals pro-regenerative genes in spinal cord injury

A switch in pdgfrb cell-derived ECM composition prevents inhibitory scarring and promotes axon regeneration in the zebrafish spinal cord

(2021) Vasiliki Tsata et al.   DEVELOPMENTAL CELL

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes

(2021) François Kroll et al.   eLife

Immune cell regulation of glia during CNS injury and disease

(2020) Andrew D. Greenhalgh et al.   NATURE REVIEWS NEUROSCIENCE

Mutagenesis of putative ciliary genes with the CRISPR/Cas9 system in zebrafish identifies genes required for retinal development

(2019) Ruikun Hu et al.   FASEB JOURNAL

Genetic control of cellular morphogenesis in Müller glia

(2019) Mark Charlton‐Perkins et al.   GLIA

Genetic compensation triggered by mutant mRNA degradation

(2019) Mohamed A. El-Brolosy et al.   NATURE

CHOPCHOP v3: expanding the CRISPR web toolbox beyond genome editing

(2019) Kornel Labun et al.   NUCLEIC ACIDS RESEARCH

Developing zebrafish disease models for in vivo small molecule screens

(2019) Pui-Ying Lam et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A zebrafish drug screening platform boosts the discovery of novel therapeutics for spinal cord injury in mammals

(2019) Diana Chapela et al.   Scientific Reports

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

Highly Efficient CRISPR-Cas9-Based Methods for Generating Deletion Mutations and F0 Embryos that Lack Gene Function in Zebrafish

(2019) Kazuyuki Hoshijima et al.   DEVELOPMENTAL CELL

A Rapid Method for Directed Gene Knockout for Screening in G0 Zebrafish

(2018) Roland S. Wu et al.   DEVELOPMENTAL CELL

Acute multi-sgRNA knockdown of KEOPS complex genes reproduces the microcephaly phenotype of the stable knockout zebrafish model

(2018) Tilman Jobst-Schwan et al.   PLoS One

A Rapid CRISPR/Cas-based Mutagenesis Assay in Zebrafish for Identification of Genes Involved in Thyroid Morphogenesis and Function

(2018) A. Trubiroha et al.   Scientific Reports

A Survey of Validation Strategies for CRISPR-Cas9 Editing

(2018) Monica F. Sentmanat et al.   Scientific Reports

An automated high-resolution in vivo screen in zebrafish to identify chemical regulators of myelination

(2018) Jason J Early et al.   eLife

Dynamic control of proinflammatory cytokines Il-1β and Tnf-α by macrophages in zebrafish spinal cord regeneration

(2018) Themistoklis M. Tsarouchas et al.   Nature Communications

Wnt signaling controls pro-regenerative Collagen XII in functional spinal cord regeneration in zebrafish

(2017) Daniel Wehner et al.   Nature Communications

mRNA processing in mutant zebrafish lines generated by chemical and CRISPR-mediated mutagenesis produces unexpected transcripts that escape nonsense-mediated decay

(2017) Jennifer L. Anderson et al.   PLoS Genetics

Dolichol-phosphate mannose synthase depletion in zebrafish leads to dystrophic muscle with hypoglycosylated α-dystroglycan

(2016) Maria Marchese et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Spinal motor neurons are regenerated after mechanical lesion and genetic ablation in larval zebrafish

(2016) Jochen Ohnmacht et al.   DEVELOPMENT

Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein complexes

(2016) Alexa Burger et al.   DEVELOPMENT

The role of secreted protein acidic and rich in cysteine (SPARC) in cardiac repair and fibrosis: Does expression of SPARC by macrophages influence outcomes?

(2016) Amy D. Bradshaw   JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY

Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9

(2016) John G Doench et al.   NATURE BIOTECHNOLOGY

Injury-induced ctgfa directs glial bridging and spinal cord regeneration in zebrafish

(2016) M. H. Mokalled et al.   SCIENCE

Rapid Reverse Genetic Screening Using CRISPR in Zebrafish

(2016) Arish N. Shah et al.   Zebrafish

A method for high-throughput PCR-based genotyping of larval zebrafish tail biopsies

(2016) Robert N. Wilkinson et al.   BIOTECHNIQUES

Sequence determinants of improved CRISPR sgRNA design

(2015) Han Xu et al.   GENOME RESEARCH

Glucocerebrosidase 1 deficientDanio reriomirror key pathological aspects of human Gaucher disease and provide evidence of early microglial activation preceding alpha-synuclein-independent neuronal cell death

(2015) Marcus Keatinge et al.   HUMAN MOLECULAR GENETICS

CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo

(2015) Miguel A Moreno-Mateos et al.   NATURE METHODS

Rapid reverse genetic screening using CRISPR in zebrafish

(2015) Arish N Shah et al.   NATURE METHODS

Comparison of T7E1 and Surveyor Mismatch Cleavage Assays to Detect Mutations Triggered by Engineered Nucleases

(2015) Léna Vouillot et al.   G3-Genes Genomes Genetics

Differential Requirement for irf8 in Formation of Embryonic and Adult Macrophages in Zebrafish

(2015) Celia E. Shiau et al.   PLoS One

Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish

(2015) Hirohito Kotani et al.   PLoS One

Radial glial progenitors repair the zebrafish spinal cord following transection

(2014) Lisa K. Briona et al.   EXPERIMENTAL NEUROLOGY

Redox and Src family kinase signaling control leukocyte wound attraction and neutrophil reverse migration

(2014) Sebastien Tauzin et al.   JOURNAL OF CELL BIOLOGY

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases

(2014) Shengdar Q Tsai et al.   NATURE BIOTECHNOLOGY

Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

(2014) James A. Gagnon et al.   PLoS One

The microglial sensome revealed by direct RNA sequencing

(2013) Suzanne E Hickman et al.   NATURE NEUROSCIENCE

Efficient multiplex biallelic zebrafish genome editing using a CRISPR nuclease system

(2013) Li-En Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Blockage of Lysophosphatidic Acid Signaling Improves Spinal Cord Injury Outcomes

(2012) Yona Goldshmit et al.   AMERICAN JOURNAL OF PATHOLOGY

The role of “anti-inflammatory” cytokines in axon regeneration

(2012) Pía M. Vidal et al.   CYTOKINE & GROWTH FACTOR REVIEWS

Iron homeostasis in astrocytes and microglia is differentially regulated by TNF-α and TGF-β1

(2012) Khizr I. Rathore et al.   GLIA

Astrocytes Control Glutamate Receptor Levels at Developing Synapses through SPARC- -Integrin Interactions

(2011) E. V. Jones et al.   JOURNAL OF NEUROSCIENCE

Control of excitatory CNS synaptogenesis by astrocyte-secreted proteins Hevin and SPARC

(2011) Hakan Kucukdereli et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

mpeg1 promoter transgenes direct macrophage-lineage expression in zebrafish

(2010) F. Ellett et al.   BLOOD

Live Imaging of Neuronal Degradation by Microglia Reveals a Role for v0-ATPase a1 in Phagosomal Fusion In Vivo

(2008) Francesca Peri et al.   CELL